DMAC2 (NM_001167867) Human Tagged ORF Clone

CAT#: RC229788

ATP5SL (Myc-DDK-tagged)-Human ATP5S-like (ATP5SL), transcript variant 1

Product Images

Circular map for RC229788

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DMAC2
• Synonyms: ATP5SL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229788 representing NM_001167867
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGGATTTGGTCTTAGAAGACCGAAGGATGAATTCCCTGCGCCTGGTCGCCCCCATGTGGAATGGGC
  GTATCAGGGGCATCCATCGCCTGGGTGCGGCAGTGGCCCCAGAGGGCAATCAGAAGAAGAAAAGGACAAT
  ACTCCAGTTCCTGACCAACTATTTCTACGATGTGGAGGCTCTGAGGGATTACTTGCTCCAAAGGGAGATG
  TACAAGGTGCATGAGAAAAATCGATCTTACACCTGGCTGGAGAAGCAACATGGTCCATACGGCGCAGGTG
  CCTTTTTCATCCTGAAGCAGGGAGGCGCAGTCAAGTTTCGAGACAAGGAGTGGATCAGGCCAGATAAGTA
  TGGCCATTTCTCTCAGGAGTTCTGGAATTTCTGTGAAGTGCCTGTCGAAGCTGTGGATGCCGGTGACTGT
  GACATCAACTACGAGGGCCTGGATAACCTCCTCCGCCTGAAGGAGCTCCAGTCCTTGTCGCTGCAGCGCT
  GCTGCCACGTGGACGACTGGTGTCTCAGCCGCCTCTACCCACTGGCCGACTCGTTGCAGGAGCTCTCGCT
  GGCCGGTTGCCCCCGCATCTCCGAACGGGGCCTCGCCTGCCTCCACCACCTCCAGAACCTCCGCAGGCTG
  GACATCTCGGACCTCCCTGCCGTGTCCAACCCTGGCCTCACTCAGATATTGGTGGAGGAGATGCTGCCCA
  ATTGCGAGGTTGTGGGAGTCGACTGGGCTGAGGGCCTGAAGTCAGGGCCGGAGGAGCAGCCTCGGGACAC
  AGCCAGCCCTGTCCCTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229788 representing NM_001167867
  Red=Cloning site Green=Tags(s)
  
  MWDLVLEDRRMNSLRLVAPMWNGRIRGIHRLGAAVAPEGNQKKKRTILQFLTNYFYDVEALRDYLLQREM
  YKVHEKNRSYTWLEKQHGPYGAGAFFILKQGGAVKFRDKEWIRPDKYGHFSQEFWNFCEVPVEAVDAGDC
  DINYEGLDNLLRLKELQSLSLQRCCHVDDWCLSRLYPLADSLQELSLAGCPRISERGLACLHHLQNLRRL
  DISDLPAVSNPGLTQILVEEMLPNCEVVGVDWAEGLKSGPEEQPRDTASPVPA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001167867
• ORF Size: 789 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001167867.2
• RefSeq ORF: 792 bp
• Locus ID: 55101
• UniProt ID: Q9NW81
• Cytogenetics: 19q13.2
• MW: 30.6 kDa
• Gene Summary: Required for the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). Involved in the assembly of the distal region of complex I.[UniProtKB/Swiss-Prot Function]