T Plastin (PLS3) (NM_001136025) Human Tagged Lenti ORF Clone
CAT#: RC227557L4
- LentiORF®
Lenti-ORF clone of PLS3 (mGFP-tagged)-Human plastin 3 (PLS3), transcript variant 2
"NM_001136025" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | T Plastin |
Synonyms | BMND18; T-plastin |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC227557).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001136025 |
ORF Size | 1890 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001136025.3 |
RefSeq Size | 3341 bp |
RefSeq ORF | 1893 bp |
Locus ID | 5358 |
UniProt ID | P13797 |
Cytogenetics | Xq23 |
MW | 70.8 kDa |
Gene Summary | Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC227557 | PLS3 (Myc-DDK-tagged)-Human plastin 3 (PLS3), transcript variant 2 |
USD 586.00 |
|
RC227557L3 | Lenti-ORF clone of PLS3 (Myc-DDK-tagged)-Human plastin 3 (PLS3), transcript variant 2 |
USD 886.00 |
|
RG227557 | PLS3 (tGFP-tagged) - Human plastin 3 (PLS3), transcript variant 2 |
USD 786.00 |
|
SC325249 | PLS3 (untagged)-Human plastin 3 (PLS3), transcript variant 2 |
USD 646.00 |
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