ZSWIM6 (NM_020928) Human Tagged ORF Clone

CAT#: RC227098

ZSWIM6 (Myc-DDK-tagged)-Human zinc finger, SWIM-type containing 6 (ZSWIM6). Note: ORF is codon optimized

Product Images

Circular map for RC227098

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZSWIM6
• Synonyms: AFND; NEDMAGA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227098 ORF sequence, codon optimized.
  Due to the complexity of NM_020928, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAACGGGGCCAGCAGCCCCCTCCTGCAAAGCGACTGTGCTGCAGGCCTGGTGGTGGCGGCGGGG
  GGGGCGGAAGCTCAGGCGGGGGTGGCGGAGCCGGTGGCGGGTATTCTTCTGCATGTAGACCCGGTCCACG
  CGCAGGAGGAGCGGCTGCAGCCGCTGCTTGCGGTGGTGGTGCCGCTCTGGGCCTGTTGCCTCCTGGGAAA
  ACGCAGTCTCCAGAATCCCTTCTTGATATAGCAGCACGCAGAGTGGCTGAAAAATGGCCCTTTCAGAGGG
  TAGAAGAGAGGTTCGAACGAATCCCGGAACCAGTCCAAAGACGCATCGTTTATTGGTCTTTCCCTAGAAG
  CGAGCGGGAAATATGCATGTATTCAAGCTTCAACACCGGCGGGGGGGCTGCCGGCGGACCCGGCGACGAC
  TCTGGAGGCGGAGGCGGAGCAGGGGGGGGGGGGGGAGGTGGCTCCAGTAGCTCTCCAGCCGCCACAAGCG
  CCGCTGCAACCTCCGCGGCCGCCGCGGCCGCGGCTGCCGCAGCTGCCGCCGCGGCGGCCGCTGGCGCCGG
  CGCTCCGAGTGTTGGTGCAGCCGGCGCCGCCGACGGCGGCGATGAAACTCGGCTTCCCTTCCGGAGGGGA
  ATAGCCCTTTTGGAAAGTGGCTGCGTGGATAACGTGCTGCAGGTTGGCTTCCACCTGAGCGGGACAGTCA
  CCGAGCCCGCCATTCAATCAGAGCCCGAGACAGTGTGCAACGTCGCCATTAGCTTCGACAGGTGTAAAAT
  TACTAGCGTTACTTGCAGCTGTGGCAACAAAGATATCTTTTATTGCGCTCACGTAGTCGCACTCAGCCTG
  TACCGGATTAGGAAACCTGACCAAGTCAAACTCCACTTGCCGATATCCGAGACACTCTTTCAGATGAACC
  GCGACCAGCTCCAGAAGTTCGTCCAGTATTTGATCACCGTGCACCACACCGAAGTACTCCCGACCGCTCA
  GAAGCTCGCAGACGAGATTCTCAGCCAGAATTCTGAGATCAATCAGGTTCACGGAGCGCCCGACCCAACT
  GCAGGAGCGAGTATCGACGACGAGAACTGTTGGCATCTGGACGAAGAACAAGTGCAGGAGCAAGTGAAAC
  TGTTCCTCAGCCAAGGAGGGTATCATGGAAGTGGGAAACAATTGAACTTGCTGTTTGCCAAGGTCCGGGA
  GATGCTCAAGATGAGAGATTCTAACGGCGCGCGCATGTTGACACTGATCACAGAGCAGTTTATGGCTGAC
  CCACGCTTGAGTCTCTGGAGACAACAGGGAACTGCTATGACCGACAAATATCGCCAGCTCTGGGACGAAC
  TGGGAGCCCTCTGGATGTGCATTGTGTTGAACCCTCACTGTAAGCTGGAACAGAAGGCAAGTTGGCTCAA
  GCAACTTAAAAAGTGGAATTCTGTGGATGTCTGCCCCTGGGAGGATGGCAATCACGGATCAGAATTGCCC
  AATTTGACTAACGCTCTGCCCCAAGGGGCAAACGCCAACCAAGACAGTTCTAACCGACCACACCGAACAG
  TCTTCACCAGAGCAATTGAGGCCTGCGATTTGCACTGGCAGGACTCTCACCTGCAGCATATTATTTCATC
  AGATCTTTACACGAATTACTGTTATCATGACGACACTGAAAACAGCTTGTTTGATTCACGCGGGTGGCCC
  TTGTGGCATGAGCACGTGCCCACAGCCTGCGCGCGCGTAGACGCACTGCGGAGCCACGGCTATCCACGGG
  AGGCCTTGCGATTGGCCATAGCCATAGTTAACACCCTGCGCCGACAACAGCAGAAACAATTGGAGATGTT
  TAGAACCCAGAAAAAAGAGCTCCCCCACAAAAATATCACCAGCATTACGAACCTGGAGGGGTGGGTTGGC
  CATCCTCTGGACCCCGTGGGCACTTTGTTTAGCAGCTTGATGGAGGCATGTAGAATCGATGACGAGAATC
  TGAGCGGGTTCTCCGACTTCACAGAGAACATGGGCCAGTGCAAGAGTTTGGAATACCAGCATCTCCCAGC
  CCACAAGTTTCTGGAAGAGGGTGAATCATATCTCACTTTGGCCGTGGAGGTAGCACTTATCGGGTTGGGA
  CAGCAGCGCATCATGCCAGATGGACTGTACACTCAAGAGAAGGTGTGTAGGAACGAAGAGCAACTCATAT
  CAAAGCTGCAAGAAATTGAGCTGGACGATACGCTGGTCAAGATTTTTAGAAAACAAGCCGTGTTTCTGCT
  CGAAGCCGGGCCATATTCAGGTCTGGGCGAGATAATTCACAGAGAGAGTGTTCCGATGCATACTTTCGCG
  AAATACCTGTTTACATCTCTGCTGCCTCATGACGCCGAACTGGCCTACAAAATTGCTCTGCGAGCCATGC
  GACTTCTGGTGCTCGAGTCAACAGCGCCCTCCGGCGATCTGACCAGACCCCACCACATCGCTAGCGTGGT
  TCCTAATCGGTATCCCCGCTGGTTTACCTTGTCACACATAGAATCCCAGCAGTGTGAGCTCGCTAGCACG
  ATGCTCACGGCTGCAAAAGGGGACGTGAGGCGCCTTGAAACCGTACTGGAAAGCATTCAGAAAAATATCC
  ATTCTAGCAGCCACATCTTTAAGCTGGCACAAGATGCTTTCAAAATAGCCACCTTGATGGACAGCCTTCC
  GGACATCACCCTGCTGAAAGTGAGCCTTGAGCTGGGACTCCAGGTCATGCGCATGACATTGTCAACACTG
  AACTGGAGGAGGAGAGAGATGGTTCGCTGGCTCGTAACTTGCGCGACAGAGGTGGGTGTCTATGCCCTTG
  ACAGCATTATGCAGACCTGGTTTACTCTCTTCACCCCTACAGAGGCTACCTCCATCGTAGCCACTACGGT
  CATGTCTAACTCCACTATAGTCAGGCTGCACTTGGATTGTCATCAGCAAGAGAAGCTTGCATCCTCAGCC
  CGAACCCTCGCTTTGCAGTGTGCTATGAAAGATCCACAGAACTGCGCCCTGAGTGCTCTCACCCTGTGCG
  AAAAAGACCACATCGCTTTCGAGACGGCGTACCAGATCGTCCTCGACGCGGCCACCACAGGGATGTCTTA
  TACTCAGCTTTTCACCATCGCGAGATACATGGAGCACAGAGGCTACCCCATGCGGGCGTACAAGTTGGCG
  ACCTTGGCTATGACACACTTGAATCTGTCTTATAACCAGGACACCCACCCAGCAATCAACGACGTACTGT
  GGGCATGCGCTTTGTCTCATTCCCTTGGCAAGAATGAACTGGCCGCGATCATTCCACTTGTCGTTAAAAG
  CGTAAAGTGTGCGACAGTTCTGTCAGATATTCTCCGGCGCTGCACACTTACTACTCCTGGTATGGTCGGA
  CTGCATGGCAGACGGAATTCAGGAAAGCTGATGAGCCTGGATAAGGCACCCCTGAGGCAGCTGCTTGATG
  CCACTATTGGCGCATATATCAATACTACACACTCACGGCTGACTCATATATCTCCACGGCACTACTCCGA
  ATTCATCGAATTTCTCAGTAAGGCTAGAGAAACTTTCTTGATGGCTCATGATGGTCATATCCAGTTCACA
  CAGTTCATTGACAACCTGAAACAGATCTACAAGGGCAAGAAAAAGTTGATGATGCTGGTGCGCGAACGGT
  TTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227098 representing NM_020928
  Red=Cloning site Green=Tags(s)
  
  MAERGQQPPPAKRLCCRPGGGGGGGGSSGGGGGAGGGYSSACRPGPRAGGAAAAAACGGGAALGLLPPGK
  TQSPESLLDIAARRVAEKWPFQRVEERFERIPEPVQRRIVYWSFPRSEREICMYSSFNTGGGAAGGPGDD
  SGGGGGAGGGGGGGSSSSPAATSAAATSAAAAAAAAAAAAAAAAGAGAPSVGAAGAADGGDETRLPFRRG
  IALLESGCVDNVLQVGFHLSGTVTEPAIQSEPETVCNVAISFDRCKITSVTCSCGNKDIFYCAHVVALSL
  YRIRKPDQVKLHLPISETLFQMNRDQLQKFVQYLITVHHTEVLPTAQKLADEILSQNSEINQVHGAPDPT
  AGASIDDENCWHLDEEQVQEQVKLFLSQGGYHGSGKQLNLLFAKVREMLKMRDSNGARMLTLITEQFMAD
  PRLSLWRQQGTAMTDKYRQLWDELGALWMCIVLNPHCKLEQKASWLKQLKKWNSVDVCPWEDGNHGSELP
  NLTNALPQGANANQDSSNRPHRTVFTRAIEACDLHWQDSHLQHIISSDLYTNYCYHDDTENSLFDSRGWP
  LWHEHVPTACARVDALRSHGYPREALRLAIAIVNTLRRQQQKQLEMFRTQKKELPHKNITSITNLEGWVG
  HPLDPVGTLFSSLMEACRIDDENLSGFSDFTENMGQCKSLEYQHLPAHKFLEEGESYLTLAVEVALIGLG
  QQRIMPDGLYTQEKVCRNEEQLISKLQEIELDDTLVKIFRKQAVFLLEAGPYSGLGEIIHRESVPMHTFA
  KYLFTSLLPHDAELAYKIALRAMRLLVLESTAPSGDLTRPHHIASVVPNRYPRWFTLSHIESQQCELAST
  MLTAAKGDVRRLETVLESIQKNIHSSSHIFKLAQDAFKIATLMDSLPDITLLKVSLELGLQVMRMTLSTL
  NWRRREMVRWLVTCATEVGVYALDSIMQTWFTLFTPTEATSIVATTVMSNSTIVRLHLDCHQQEKLASSA
  RTLALQCAMKDPQNCALSALTLCEKDHIAFETAYQIVLDAATTGMSYTQLFTIARYMEHRGYPMRAYKLA
  TLAMTHLNLSYNQDTHPAINDVLWACALSHSLGKNELAAIIPLVVKSVKCATVLSDILRRCTLTTPGMVG
  LHGRRNSGKLMSLDKAPLRQLLDATIGAYINTTHSRLTHISPRHYSEFIEFLSKARETFLMAHDGHIQFT
  QFIDNLKQIYKGKKKLMMLVRERFG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020928
• ORF Size: 3645 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020928.1, NP_065979.1
• RefSeq Size: 5518 bp
• RefSeq ORF: 3648 bp
• Locus ID: 57688
• UniProt ID: Q9HCJ5
• Cytogenetics: 5q12.1
• MW: 133.5 kDa
• Gene Summary: The protein encoded by this gene contains a zinc finger SWI2/SNF2 and MuDR (SWIM) domain. Proteins with SWIM domains have been found in a diverse number of species and are predicted to interact with DNA or proteins. Mutations in this gene result in acromelic frontonasal dysostosis. [provided by RefSeq, Apr 2017]