CMKLR1 (NM_001142345) Human Tagged ORF Clone

CAT#: RC227056

CMKLR1 (Myc-DDK-tagged)-Human chemokine-like receptor 1 (CMKLR1), transcript variant 4

Product Images

Circular map for RC227056

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CMKLR1
• Synonyms: CHEMERINR; ChemR23; DEZ; RVER1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227056 representing NM_001142345
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAATGGAGGATGAAGATTACAACACTTCCATCAGTTACGGTGATGAATACCCTGATTATTTAGACT
  CCATTGTGGTTTTGGAGGACTTATCCCCCTTGGAAGCCAGGGTGACCAGGATCTTCCTGGTGGTGGTCTA
  CAGCATCGTCTGCTTCCTCGGGATTCTGGGCAATGGTCTGGTGATCATCATTGCCACCTTCAAGATGAAG
  AAGACAGTGAACATGGTCTGGTTCCTCAACCTGGCAGTGGCAGATTTCCTGTTCAACGTCTTCCTCCCAA
  TCCATATCACCTATGCCGCCATGGACTACCACTGGGTTTTCGGGACAGCCATGTGCAAGATCAGCAACTT
  CCTTCTCATCCACAACATGTTCACCAGCGTCTTCCTGCTGACCATCATCAGCTCTGACCGCTGCATCTCT
  GTGCTCCTCCCTGTCTGGTCCCAGAACCACCGCAGCGTTCGCCTGGCTTACATGGCCTGCATGGTCATCT
  GGGTCCTGGCTTTCTTCTTGAGTTCCCCATCTCTCGTCTTCCGGGACACAGCCAACCTGCATGGGAAAAT
  ATCCTGCTTCAACAACTTCAGCCTGTCCACACCTGGGTCTTCCTCGTGGCCCACTCACTCCCAAATGGAC
  CCTGTGGGGTATAGCCGGCACATGGTGGTGACTGTCACCCGCTTCCTCTGTGGCTTCCTGGTCCCAGTCC
  TCATCATCACAGCTTGCTACCTCACCATCGTGTGCAAACTGCAGCGCAACCGCCTGGCCAAGACCAAGAA
  GCCCTTCAAGATTATTGTGACCATCATCATTACCTTCTTCCTCTGCTGGTGCCCCTACCACACACTCAAC
  CTCCTAGAGCTCCACCACACTGCCATGCCTGGCTCTGTCTTCAGCCTGGGTTTGCCCCTGGCCACTGCCC
  TTGCCATTGCCAACAGCTGCATGAACCCCATTCTGTATGTTTTCATGGGTCAGGACTTCAAGAAGTTCAA
  GGTGGCCCTCTTCTCTCGCCTGGTCAATGCTCTAAGTGAAGATACAGGCCACTCTTCCTACCCCAGCCAT
  AGAAGCTTTACCAAGATGTCATCAATGAATGAGAGGACTTCTATGAATGAGAGGGAGACCGGCATGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227056 representing NM_001142345
  Red=Cloning site Green=Tags(s)
  
  MRMEDEDYNTSISYGDEYPDYLDSIVVLEDLSPLEARVTRIFLVVVYSIVCFLGILGNGLVIIIATFKMK
  KTVNMVWFLNLAVADFLFNVFLPIHITYAAMDYHWVFGTAMCKISNFLLIHNMFTSVFLLTIISSDRCIS
  VLLPVWSQNHRSVRLAYMACMVIWVLAFFLSSPSLVFRDTANLHGKISCFNNFSLSTPGSSSWPTHSQMD
  PVGYSRHMVVTVTRFLCGFLVPVLIITACYLTIVCKLQRNRLAKTKKPFKIIVTIIITFFLCWCPYHTLN
  LLELHHTAMPGSVFSLGLPLATALAIANSCMNPILYVFMGQDFKKFKVALFSRLVNALSEDTGHSSYPSH
  RSFTKMSSMNERTSMNERETGML
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142345
• ORF Size: 1119 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142345.1, NP_001135817.1
• RefSeq ORF: 1122 bp
• Locus ID: 1240
• UniProt ID: Q99788
• Cytogenetics: 12q23.3
• Protein Families: Druggable Genome, GPCR, Transmembrane
• MW: 42.1 kDa
• Gene Summary: Receptor for the chemoattractant adipokine chemerin/RARRES2 and for the omega-3 fatty acid derived molecule resolvin E1. Interaction with RARRES2 induces activation of intracellular signaling molecules, such as SKY, MAPK1/3 (ERK1/2), MAPK14/P38MAPK and PI3K leading to multifunctional effects, like, reduction of immune responses, enhancing of adipogenesis and angionesis. Resolvin E1 down-regulates cytokine production in macrophages by reducing the activation of MAPK1/3 (ERK1/2) and NF-kappa-B. Positively regulates adipogenesis and adipocyte metabolism. Acts as a coreceptor for several SIV strains (SIVMAC316, SIVMAC239, SIVMACL7E-FR and SIVSM62A), as well as a primary HIV-1 strain (92UG024-2).[UniProtKB/Swiss-Prot Function]