ZNF181 (NM_001145665) Human Tagged ORF Clone

CAT#: RC226990

ZNF181 (Myc-DDK-tagged)-Human zinc finger protein 181 (ZNF181), transcript variant 2. Note: ORF is codon optimized

Product Images

Circular map for RC226990

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF181
• Synonyms: HHZ181
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226990 ORF sequence, codon optimized.
  Due to the complexity of NM_001145665, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCAGGTAACTTTCAACGATGTTGCCATAGATTTTACGCATGAAGAATGGGGCTGGCTGTCTAGTG
  CACAGAGGGACCTTTACAAGGACGTGATGGTCCAGAATTACGAGAACCTTGTTTCAGTAGGCCTGTCAGT
  CACCAAGCCATACGTGATCACGCTTCTTGAAGATGGCAAAGAACCATGGATGATGGAGAAGAAGCTTTCT
  AAAGGCATGATCCCCGATTGGGAAAGCCGGTGGGAGAACAAAGAACTTTCCACCAAGAAAGATAACTACG
  ATGAAGATTCTCCCCAGACTGTTATTATTGAGAAGGTGGTGAAACAGAGTTACGAGTTTTCTAATAGCAA
  GAAAAACTTGGAATATATCGAGAAGTTGGAAGGGAAGCACGGATCTCAAGTGGACCATTTTAGACCTGCA
  ATTCTTACCTCCAGGGAGTCACCCACTGCCGATTCAGTGTACAAATATAACATCTTCCGCTCCACATTCC
  ATTCCAAGAGTACCCTCTCAGAACCTCAGAAAATTTCAGCTGAGGGTAACTCTCACAAGTACGACATCCT
  GAAAAAGAACCTCCCTAAGAAGAGTGTGATTAAAAACGAAAAGGTCAACGGCGGCAAGAAGTTGCTGAAT
  AGCAATAAGTCTGGTGCCGCCTTCAGCCAAGGTAAGTCCTTGACACTGCCACAGACCTGCAATCGGGAAA
  AAATCTACACCTGTAGCGAATGTGGAAAGGCGTTTGGCAAGCAGAGCATTCTGAATCGACATTGGCGAAT
  TCACACAGGAGAGAAGCCCTACGAATGCAGAGAGTGCGGCAAGACGTTCAGCCACGGGAGCTCACTGACC
  AGGCACCTCATCAGTCACAGCGGAGAGAAGCCGTATAAGTGCATCGAATGTGGCAAGGCCTTTTCCCACG
  TGAGCTCACTGACTAACCACCAAAGTACGCACACCGGGGAAAAACCTTATGAATGTATGAATTGCGGCAA
  ATCTTTCTCCCGCGTTTCACATCTGATAGAGCACCTGCGAATCCACACCCAGGAAAAGCTGTACGAGTGT
  CGAATCTGTGGTAAGGCCTTTATCCACCGCTCATCACTGATTCACCACCAGAAGATCCATACTGGAGAGA
  AACCGTACGAGTGCAGAGAATGCGGGAAGGCTTTTTGCTGCTCCAGTCACCTCACGCGGCACCAACGCAT
  TCACACAATGGAAAAACAGTATGAGTGTAATAAGTGTCTGAAAGTGTTCTCTTCCCTCTCTTTTTTGGTG
  CAACACCAGAGTATCCACACAGAAGAAAAGCCCTTTGAGTGCCAGAAGTGTCGAAAGAGTTTCAACCAAC
  TGGAGTCCCTCAACATGCATCTGAGAAATCATATTAGGTTGAAACCATATGAATGTTCAATTTGTGGGAA
  AGCCTTTAGCCATCGGAGTTCACTCCTCCAGCACCACCGAATCCATACAGGGGAAAAGCCATATGAATGC
  ATCAAATGCGGGAAAACATTCTCCTGCTCATCCAACTTGACTGTGCACCAGCGCATACACACCGGCGAAA
  AGCCATACAAGTGCAACGAATGTGGAAAAGCTTTTAGTAAAGGATCTAACCTGACAGCTCACCAGAGGGT
  TCACAATGGCGAGAAGCCTAATTCCGTGGTCAGTGTCGAAAAACCACTGGACTACATGAACCACTATACT
  TGTGAGAAAAGCTACCGGAGGGAGACAGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226990 representing NM_001145665
  Red=Cloning site Green=Tags(s)
  
  MPQVTFNDVAIDFTHEEWGWLSSAQRDLYKDVMVQNYENLVSVGLSVTKPYVITLLEDGKEPWMMEKKLS
  KGMIPDWESRWENKELSTKKDNYDEDSPQTVIIEKVVKQSYEFSNSKKNLEYIEKLEGKHGSQVDHFRPA
  ILTSRESPTADSVYKYNIFRSTFHSKSTLSEPQKISAEGNSHKYDILKKNLPKKSVIKNEKVNGGKKLLN
  SNKSGAAFSQGKSLTLPQTCNREKIYTCSECGKAFGKQSILNRHWRIHTGEKPYECRECGKTFSHGSSLT
  RHLISHSGEKPYKCIECGKAFSHVSSLTNHQSTHTGEKPYECMNCGKSFSRVSHLIEHLRIHTQEKLYEC
  RICGKAFIHRSSLIHHQKIHTGEKPYECRECGKAFCCSSHLTRHQRIHTMEKQYECNKCLKVFSSLSFLV
  QHQSIHTEEKPFECQKCRKSFNQLESLNMHLRNHIRLKPYECSICGKAFSHRSSLLQHHRIHTGEKPYEC
  IKCGKTFSCSSNLTVHQRIHTGEKPYKCNECGKAFSKGSNLTAHQRVHNGEKPNSVVSVEKPLDYMNHYT
  CEKSYRRETV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145665
• ORF Size: 1710 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145665.1, NP_001139137.1
• RefSeq Size: 2948 bp
• RefSeq ORF: 1713 bp
• Locus ID: 339318
• UniProt ID: Q2M3W8
• Cytogenetics: 19q13.11
• MW: 65.8 kDa
• Gene Summary: Zinc finger proteins have been shown to interact with nucleic acids and to have diverse functions. The zinc finger domain is a conserved amino acid sequence motif containing 2 specifically positioned cysteines and 2 histidines that are involved in coordinating zinc. Kruppel-related proteins form 1 family of zinc finger proteins. See MIM 604749 for additional information on zinc finger proteins.[supplied by OMIM, Jul 2003]