FER1L5 (NM_001113382) Human Tagged ORF Clone

CAT#: RC226448

FER1L5 (Myc-DDK-tagged)-Human fer-1-like 5 (C. elegans) (FER1L5)

Product Images

Circular map for RC226448

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FER1L5
• Synonyms: fer-1-like 5; fer-1-like 5 (C. elegans)
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226448 representing NM_001113382
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCGGCTTGTGGTGCAGTCGGCCAAGATTGACCCACCACTAGCCCCACTACCCAGGCCCTGCATGT
  CCATCGACTTCAGAGATATCAAGAAAAGAACTCGTGTGGTGGAAGGGAATGATCCCGTGTGGAATGAGAC
  CCTAATCTGGCACCTCTGGAACCGCCCCCTGGAAAATGACTCCTTCCTGCAAGTCACCCTTCAGGACATG
  GGCTCACAAAAGAAAGAAAGATTCATTGGCCTGGCCACAGTACTGCTCAAGCCATTGTTGAAACAACCAA
  GTGAGGTCCTTTTTGTGAAGGACTTGACCCTGCTCAACCATTCCATGAAGCCCACAGATTGTACTGTCAC
  CCTACAGGTGGCCCACATGAGCAACCAGGATATTGAGAAGACAGGAGCTGAAGACCACCTGGGCATAACG
  GCAAGAGAGGCAGCCAGTCAGAAACTGATGGTCCCTGGCTCCACTGCGCACAGGGCTCTGTCCTCAAAGC
  CTCAGCACTTTCAGGTTCGAGTGAAGGTGTTTGAAGCCCGACAGCTCATGGGCAACAACATCAAACCAGT
  GGTGAAGGTGTCCATCGCAGGCCAGCAGCACCAGACACGCATCAAGATGGGAAACAACCCTTTCTTTAAT
  GAGATCTTCTTCCAGAATTTTCATGAGGTTCCTGCAAAGTTCTTTGATGAGACCATCTTAATCCAGACAG
  ATATTGGGTTTATCTACCATTCTCCAGGTCACACACTCCTAAGGAAATGGCTAGGCCTCTGCCAGCCAAA
  TAACCCTGGCAGTGGTGTGACAGGCTACCTGAAAGTCACCATCTATGCCCTCGGTGTGGGAGACCAGGCC
  CTGATAGATCAAAAGCTGCTCTATGGCACCGATGACACCGATATTCAGATCTTCAAGTCAGCGGTAGTCC
  CGATCAACATGGCTTACTTACAGCTCTTCATCTACTGCGCAGAGGACCTTCACCTCAAGAAACACCAGTC
  AGTGAATCCTCAGTTGGAGGTGGAACTAATTGGGGAAAAGCTCAGGACACACATGCAGACCCAAACCGAC
  AACCCGATATGGAACCAGATCCTGACCTTCCGGATTCAGCTACCCTGCCTCTCCAGCTACATCAAGTTCA
  GAGTCTTGGACTGCCGCAAGAAGGACTGCCCGGATGAGATTGGGACTGCCAGCCTGTCCCTCAACCAGAT
  CTCGTCCACCGGAGAAGAGATAGAAGGCAAGCAAAGCCTCGAGCCCACTTCCTACACCCCTCGAGTGTAC
  TCCGGCTTCCTGCCCTGCTTTGGCCCCAGCTTCCTGACTCTGCATGGGGGTAAAAAGGCCCCTTTCAGGA
  TCCAGGAAGAAGGCGCTTGTATTCCCGACTCTGTTAGGGATGGTTTAGCTTATCGAGGCCGAGTCTTCCT
  GGAGTTAATCACCCAAATCAAGTCCTATCAAGACTCCACGATAAAGGATCTCTCCCATGAAGTGACCAGG
  ATAGAGAAGCACCAGAACCGCCAAAAGTATGGGCTGTGCGTCATCTTCCTTTCCTGTACCATGATGCCCA
  ACTTTAAAGAGCTGATCCATTTCGAGGTCAGCATCGGTCACTATGGGAACAAGATGGACCTGAATTACAA
  GCCTCTAGTCTCAAGCACACCGTACAGCCCAGTGATATATGATGGGAACATCTACCATTATGTGCCCTGG
  TACAACACCAAGCCTGTCGTGGCCGTGACCTCCAACTGGGAGGACGTCAGCTTCCGCATGAACTGCCTCA
  ACCTCCTCCACTTCACTCGGGACCGCCTGAAAGCCAACCTGGACACCCTGAAATCCACGCGGAATCCGAA
  GGATCCAGCTCTCCTCTACCAGTGGGAGAAACTGCTGAGGGAGCTGGCAGAGGACTGCAAGCGCCCTCTG
  CCCTGCATGACCTATCAGCCCAAAGCCACCAGCCTGGACAGGAAGAGGTGGCAGCTCCGCAGCCTCCTCC
  TGCAGGAACTGGCCCAAAAGGCCAAGCAAGCCAAGCCCAAGGACATGGTGGCCACAGCGGAGGACTGGCT
  GTACCGCCTCAACACCGTGCTCCCTGAGCCCCAGATGGGCCTCCCTGACGTGATGATTTGGCTGGTGGCC
  AAGGAGCAGCGAGTGGCCTATGCACAGGTGCCTGCCCACTCCGTCCTCTTCTCCCCGGCAGGGGCTCTGC
  ACTCCGGCAGGCTCTGTGGGAAGATACAGACACTCTTCCTACAGTACCCAGAGGGTGAAGGACAGAAGGA
  TGTGCTCCCAGCTCACCTCCGGGTCTGCATGTGGCTTGGCAATGTCACAGACAGCAAGGACCTGCAGCTG
  CTCCGCCAGGGTGACACAGCGGTGTACGCCGAGATGTATGAGAATCAGGCCAAGTATAAAGACCAGTGGG
  GGCAGCAGGGGCTGTATCACTGCCCCAACTTCTCGGATGTCATGGGGAACAAGACCCTCCCCATGACGGA
  TTTCCAACCACCCCTGGGATGGCACTGGCAGGACAGCTGGACAGTGGAACCTCAGAGAAGGCTCCTCCTG
  GACATAGACATCAACAAGAGCCAGGTGCTGGAGGAGGTATATGAGAACCAGGGCCGTGACACCAGAGGGG
  CCTGGGGGCCTGCCGCCATCCCAAACACAGACGTGAATGGACAGCCCATGGAGGCCCGGGAGAACGTGAA
  GTGCCCCCAAGGCTGGCACTTTAAGAAGGACTGGGTGGTGGAGCTGAACCACGCAGTGGACAGCTGGGAG
  TATGGAGTGGGGATCCCACCGTCGGGCCTGCCCCAGGTCTGGAGCCCGGTGGAGAAGACCTACCACTCGT
  GCCGCCGCCGGCGCTGGGCGCGTGTGCGCTTCAGGAACCATGGGGAGCTGAGCCACGAGCAGGAGACCCT
  CTCCTTCCTGCAGCTGGGCCTGGCCAAGGGCGAGGAGGAGGGCTGGGAGTATGACACCTTCGGCTCCAAG
  TTCCACCTCAACCCTCAGCCCCAGAGCCGGTTCCGCCGCCGCTGCTGGCGCCGCAGGCTGGCCCCCAACA
  AGGACAAGGGCATCGCGCCCATATTCCTCCTGGAGGGGTCCTTGGCTTGCGAGGGTGGCAGTGTGAGGGA
  GGAGGGTGACTGGGCCTGGGCTAGAGGAAACAGGAGCAGCACCCAAGAGCCTCAGGGGAGACTGTCCTGG
  GTACAGGCTATGGATCTGAAATACCACGCTGGGAAGGAAGAGGACAGCAAGACATGGCCATGGGGTCTGG
  ACAGACAGTTCAGGGACCCCCAGAGGCAGGACACCCGGCCCCCCAACTTGCCCTTCATCTACTGCACCTT
  CAATAAGCCCCACTACTACCAGCTCTTCTGCTACATCTACCAGGCCCGGAACCTGGTGTCCAATCAGATC
  CTGACATTCCAAGGGCCCTTCATTCGGGTGGTCTTCCTGAACCACAGCCAGTGCACCCAAACCCTGAGGA
  GCTCTGCAGGCCCCACATGGGCCCAGACACTCATCTTCCAGCACCTCCTTCTGTACGAGAACCCACAGGA
  CACCAAAGAGAGCCCACCGCTTGTGGTGCTGGAGCTGTGGCAGCGTGACTTCTGGGGCAAGGAGAGCTTG
  TGGGGACGGAGCGTGTGGCCCCCAATGGTCTGGCTGGATCTCCAGGACCGGATCCTGCCCCCCATGAGGT
  GGCATCCCCTTGTAAAGGAGTTGGGGAAGGAAGAGGGCGAGATCTTGGCATCCTGTGAGCTGATCCTCCA
  GACTGAGAAGCTTGGAGAGAAGCAGCTGCCTATCTTAAGCGTTCCCTGGAAGAATGGGGCATACACACTC
  CCCAAGAGCATCCAGCCCACGATAAAGAGGATGGCCATTGAGATCCTGGCCTGGGGCCTTCGGAACATGA
  AGAAGGCGAGCTCCCCCCAGCTCCTGGTGGAATTCGGGGAAGAGTCCCTGAGGACAGAACCCATCAGGGA
  CTTTCAGACCAACCCCAACTTCCCCGAGTCTGAGTCTGTCCTAGTCCTCACAGTGCTCATGCCGACGGAG
  GAGGCCTATGCACTGCCCCTCGTGGTGAAGGTGGTAGACAACTGGGCCTTCGGCCAGCAGACCGTGACGG
  GCCAGGCCAACATCGACTTCCTCCAGCCCTACTTCTGTGACCCCTGGGCTCAAGACTATATGCACCCAAA
  GCTTCCAACGCTGTCTGAGAAGAAGCACCAAGACTTCCTAGGCTACCTCTACAGAAAGTTCTGGTTCAAG
  TCCAGTAAAGCAGAGGATGAGTATGAGCATGAGGTGGACTGGTGGAGCAAGCTGTTCTGGGCCACAGATG
  AGCACAAGTCCCTGAAGTACAAGTACAAAGACTACCACACCCTCAAGGTGTATGAGTGTGAGCTGGAGGC
  CGTGCCAGCCTTCCAGGGCCTGCAGGACTTCTGCCAGACCTTCAAACTCTACCAGGAGCAGCCCAAGTTG
  GACAGCCCCGTGGTAGGGGAGTTCAAGGGCCTTTTCCGCATCTACCCCTTTCCTGAGAATCCAGAAGCCC
  CAAAGCCCCCGCTGCAGTTCTTGGTTTGGCCAGAGAGAGAGGACTTCCCCCAGCCGTGCTTGGTGCGGGT
  GTACATGGTACGAGCCATCAACCTGCAGCCCCAGGACTACAATGGCCTGTGTGACCCTTATGTGATCCTG
  AAACTGGGCAAGACAGAGCTTGGCAACCGGGACATGTACCAGCCCAACACTCTGGATCCCATCTTTGGCA
  TGATGTTTGAACTCACCTGCAACATACCCCTGGAGAAGGACCTAGAGATCCAGCTCTATGACTTCGACCT
  ATTTTCACCTGATGATAAGATAGGAACCACAGTCATCGACCTTGAAAACCGACTCCTATCTGGCTTTGGA
  GCTCATTGTGGGCTCTCCAAATCCTACTGCCAGTCAGGGCCCTTTAGATGGCGGGATCAGATGCCCCCAA
  GCTACCTCCTAGAACGCTATGCCAAGCGGAAAGGGCTACCTCCGCCTCTGTTCAGTCCTGAGGAAGATGC
  TGTTTTCTATAATGGGAAAAAGTTCAAGCTGCAAAGCTTTGAGCCCAAAACCCCTACTGTTCATGGTTTG
  GGACCCAAGAAGGAACGCCTTGCACTGTACCTCCTGCACACCCAGGGGCTGGTACCTGAGCACGTGGAGA
  CCCGCACACTGTACAGCCACAGCCAGCCAGGCATCGACCAGGGAAAGGTGCAAATGTGGGTGGACATCTT
  CCCCAAGAAGCTGGGGCCTCCTGGCCCCCAAGTCAACATCAACCCCAGAAAGCCTAAACGCAAAGCCTCA
  GAGCACAGTGGCCACAGGTATGAGCTGCGATGCATCATCTGGAAGACTGCCAATGTGGACCTGGTGGATG
  ACAATTTAAGTAGAGAGAAGACGAGCGACATCTACATCAAAGGGTGGTTATACGGGCTGGAGAAGGACAT
  GCAGAAGACAGACATCCACTACCACTCGCTGACTGGGGAGGCCGACTTCAACTGGCGGTTCATCTTTACC
  ATGGACTACCTGGCGGCGGAGCGCACGTGTGTCCAGAGCCAGAAGGATTACATATGGAGCCTGGATGCCA
  CGTCCATGAAGTTCCCAGCCCGACTTATCATCCAGGTCTGGGACAATGACATCTTCTCCCCCGACGACTT
  CCTAGGGGTCCTGGAGCTGGATTTGTCTGACATGCCCCTCCCGGCTCGGCACGCCAAGCAGTGCTCCATC
  AGGATGATGGACGCCGACCCCAAGTGGCCCTATTTCATCCAATACAAGCACTTCTCCCTCTTTAAGAAGA
  AGACTGTGACTGGCTGGTGGCCTTGCCAGGTCCTCGATGGTGGCAAATGGCGCTTGTCGGGCAAGGTGAA
  GATGAGCCTGGAGATTCTGTCAGAGAAGGAAGCCTTAATCAAGCCAGCCGGGCGAGGCCAGTCGGAACCC
  AACCAGTACCCCACACTTCATCCTCCCCTACGCACCAACACCTCTTTCACGTGGCTGCGGTCACCAGTTC
  AAAACTTCTGCTATATTTTCTGGAAACGCTATCGCTTCAAACTCATAGCCTTTATGGTCATATCGATTAT
  AGCACTTATGCTGTTTAACTTCATCTATTCAGCTCCGCACTATTTGGCCATGAGCTGGATCAAACCTCAA
  CTTCAGCTGTATCCTCCCATTAAAATATTCAATATCATCAATTCACTAAACACCAGCAACGCCAGCTCTT
  CCATCCTTCCCACCCAGGATCCAAACCTAAAGCCTACAATAGACCATGAGTGGAAACTCCACCCAGGACC
  CACAAATCACCTGAGTGATATTTTCCCAGAACTTCCAGCCCCAGGAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226448 representing NM_001113382
  Red=Cloning site Green=Tags(s)
  
  MLRLVVQSAKIDPPLAPLPRPCMSIDFRDIKKRTRVVEGNDPVWNETLIWHLWNRPLENDSFLQVTLQDM
  GSQKKERFIGLATVLLKPLLKQPSEVLFVKDLTLLNHSMKPTDCTVTLQVAHMSNQDIEKTGAEDHLGIT
  AREAASQKLMVPGSTAHRALSSKPQHFQVRVKVFEARQLMGNNIKPVVKVSIAGQQHQTRIKMGNNPFFN
  EIFFQNFHEVPAKFFDETILIQTDIGFIYHSPGHTLLRKWLGLCQPNNPGSGVTGYLKVTIYALGVGDQA
  LIDQKLLYGTDDTDIQIFKSAVVPINMAYLQLFIYCAEDLHLKKHQSVNPQLEVELIGEKLRTHMQTQTD
  NPIWNQILTFRIQLPCLSSYIKFRVLDCRKKDCPDEIGTASLSLNQISSTGEEIEGKQSLEPTSYTPRVY
  SGFLPCFGPSFLTLHGGKKAPFRIQEEGACIPDSVRDGLAYRGRVFLELITQIKSYQDSTIKDLSHEVTR
  IEKHQNRQKYGLCVIFLSCTMMPNFKELIHFEVSIGHYGNKMDLNYKPLVSSTPYSPVIYDGNIYHYVPW
  YNTKPVVAVTSNWEDVSFRMNCLNLLHFTRDRLKANLDTLKSTRNPKDPALLYQWEKLLRELAEDCKRPL
  PCMTYQPKATSLDRKRWQLRSLLLQELAQKAKQAKPKDMVATAEDWLYRLNTVLPEPQMGLPDVMIWLVA
  KEQRVAYAQVPAHSVLFSPAGALHSGRLCGKIQTLFLQYPEGEGQKDVLPAHLRVCMWLGNVTDSKDLQL
  LRQGDTAVYAEMYENQAKYKDQWGQQGLYHCPNFSDVMGNKTLPMTDFQPPLGWHWQDSWTVEPQRRLLL
  DIDINKSQVLEEVYENQGRDTRGAWGPAAIPNTDVNGQPMEARENVKCPQGWHFKKDWVVELNHAVDSWE
  YGVGIPPSGLPQVWSPVEKTYHSCRRRRWARVRFRNHGELSHEQETLSFLQLGLAKGEEEGWEYDTFGSK
  FHLNPQPQSRFRRRCWRRRLAPNKDKGIAPIFLLEGSLACEGGSVREEGDWAWARGNRSSTQEPQGRLSW
  VQAMDLKYHAGKEEDSKTWPWGLDRQFRDPQRQDTRPPNLPFIYCTFNKPHYYQLFCYIYQARNLVSNQI
  LTFQGPFIRVVFLNHSQCTQTLRSSAGPTWAQTLIFQHLLLYENPQDTKESPPLVVLELWQRDFWGKESL
  WGRSVWPPMVWLDLQDRILPPMRWHPLVKELGKEEGEILASCELILQTEKLGEKQLPILSVPWKNGAYTL
  PKSIQPTIKRMAIEILAWGLRNMKKASSPQLLVEFGEESLRTEPIRDFQTNPNFPESESVLVLTVLMPTE
  EAYALPLVVKVVDNWAFGQQTVTGQANIDFLQPYFCDPWAQDYMHPKLPTLSEKKHQDFLGYLYRKFWFK
  SSKAEDEYEHEVDWWSKLFWATDEHKSLKYKYKDYHTLKVYECELEAVPAFQGLQDFCQTFKLYQEQPKL
  DSPVVGEFKGLFRIYPFPENPEAPKPPLQFLVWPEREDFPQPCLVRVYMVRAINLQPQDYNGLCDPYVIL
  KLGKTELGNRDMYQPNTLDPIFGMMFELTCNIPLEKDLEIQLYDFDLFSPDDKIGTTVIDLENRLLSGFG
  AHCGLSKSYCQSGPFRWRDQMPPSYLLERYAKRKGLPPPLFSPEEDAVFYNGKKFKLQSFEPKTPTVHGL
  GPKKERLALYLLHTQGLVPEHVETRTLYSHSQPGIDQGKVQMWVDIFPKKLGPPGPQVNINPRKPKRKAS
  EHSGHRYELRCIIWKTANVDLVDDNLSREKTSDIYIKGWLYGLEKDMQKTDIHYHSLTGEADFNWRFIFT
  MDYLAAERTCVQSQKDYIWSLDATSMKFPARLIIQVWDNDIFSPDDFLGVLELDLSDMPLPARHAKQCSI
  RMMDADPKWPYFIQYKHFSLFKKKTVTGWWPCQVLDGGKWRLSGKVKMSLEILSEKEALIKPAGRGQSEP
  NQYPTLHPPLRTNTSFTWLRSPVQNFCYIFWKRYRFKLIAFMVISIIALMLFNFIYSAPHYLAMSWIKPQ
  LQLYPPIKIFNIINSLNTSNASSSILPTQDPNLKPTIDHEWKLHPGPTNHLSDIFPELPAPGD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001113382
• ORF Size: 6279 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001113382.1, NP_001106853.1
• RefSeq ORF: 6282 bp
• Locus ID: 90342
• Cytogenetics: 2q11.2
• Protein Families: Transmembrane
• MW: 241.8 kDa
• Gene Summary: Plays a role in myoblast fusion; probable mediator of endocytic recycling for membrane trafficking events during myotube formation.[UniProtKB/Swiss-Prot Function]