TBC1D2B (NM_144572) Human Tagged ORF Clone

CAT#: RC226280

TBC1D2B (Myc-DDK-tagged)-Human TBC1 domain family, member 2B (TBC1D2B), transcript variant 1

Product Images

Circular map for RC226280

• 

  Western blot validation of overexpression lysate (Cat# LY430114) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226280 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TBC1D2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226280 representing NM_144572
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGGGGCCGGAGCCCGGGCGGAGGAGGGCGGCGGCGGCGGCGAGGGCGCGGCGCAGGGGGCGGCCG
  CGGAGCCCGGGGCGGGTCCGGCGCGGGAGCCAGCGCGGCTGTGTGGCTATCTGCAGAAGCTGTCGGGCAA
  GGGCCCCCTGCGTGGCTACCGCAGCCGCTGGTTCGTGTTCGACGCGCGCCGCTGCTACCTTTACTATTTC
  AAGAGTCCGCAGGACGCGCTGCCCCTCGGCCACTTGGACATCGCGGACGCCTGCTTCAGCTACCAGGGCC
  CCGACGAGGCGGCGGAGCCGGGCACGGAGCCGCCCGCGCACTTCCAGGTGCACAGCGCGGGAGCCGTCAC
  GGTGCTCAAGGCTCCCAATCGTCAACTCATGACTTACTGGTTACAGGAGCTTCAGCAGAAGAGATGGGAA
  TATTGTAACAGTCTTGACATGGTCAAGTGGGACAGCAGGACCTCTCCAACTCCCGGGGATTTTCCTAAGG
  GTCTTGTAGCCAGAGATAACACTGATTTAATTTACCCACACCCAAATGCTTCTGCAGAAAAAGCCAGAAA
  TGTCCTAGCTGTGGAGACTGTGCCTGGAGAGCTGGTGGGAGAACAAGCTGCAAATCAGCCCGCCCCAGGG
  CATCCAAATTCCATTAATTTTTACTCTTTGAAACAGTGGGGCAATGAGCTCAAGAATTCGATGTCTTCTT
  TCCGTCCTGGGAGAGGACATAATGATAGTCGGAGGACTGTGTTTTATACCAATGAAGAGTGGGAACTTTT
  AGACCCAACCCCTAAGGACCTAGAGGAGTCCATAGTACAGGAAGAAAAGAAGAAGCTGACCCCTGAAGGA
  AACAAAGGAGTAACTGGCTCAGGATTCCCCTTTGATTTTGGACGTAACCCCTACAAAGGAAAGCGCCCTT
  TGAAAGACATAATTGGGTCGTACAAAAATCGTCACAGCAGTGGTGACCCTTCAAGTGAAGGCACATCAGG
  CAGTGGCAGCGTCAGCATCAGGAAGCCGGCCTCCGAAATGCAACTGCAGGTCCAGAGCCAGCAGGAAGAG
  CTGGAACAGTTAAAGAAAGACCTGTCCAGTCAGAAGGAGCTTGTTCGACTGCTCCAGCAGACAGTCCGGT
  CATCCCAGTATGACAAGTATTTCACAAGCAGCCGGCTCTGTGAGGGGGTCCCAAAGGACACGCTCGAGCT
  TCTGCACCAAAAGGATGATCAGATTCTGGGCCTTACCAGCCAGCTGGAGAGGTTCAGCTTGGAGAAGGAG
  AGTCTTCAGCAGGAAGTAAGGACGCTGAAGAGCAAAGTGGGCGAGCTCAACGAGCAGCTGGGAATGCTCA
  TGGAGACCATCCAAGCCAAGGACGAGGTCATCATCAAGCTCAGCGAGGGCGAGGGCAACGGGCCTCCTCC
  CACCGTGGCGCCCAGCTCCCCTTCGGTTGTGCCTGTTGCCAGGGACCAGCTGGAACTGGACAGGCTGAAA
  GATAATCTACAGGGGTACAAAACCCAAAACAAATTTCTAAATAAGGAGATTTTGGAACTCTCAGCTCTAC
  GAAGAAATGCAGAAAGGAGAGAGAGGGATCTGATGGCAAAGTATTCTAGCCTGGAAGCCAAGCTCTGCCA
  GATAGAAAGTAAATACCTGATATTGCTCCAAGAAATGAAGACACCAGTGTGCTCAGAAGACCAGGGGCCC
  ACCCGGGAGGTCATAGCCCAGTTGCTGGAGGATGCTCTGCAGGTTGAGAGCCAAGAGCAGCCGGAGCAAG
  CATTTGTTAAACCTCATCTTGTCAGTGAATATGATATTTATGGGTTCAGGACTGTACCTGAGGATGATGA
  GGAAGAGAAATTGGTTGCCAAGGTCCGCGCGTTGGATCTGAAGACTCTCTACCTCACAGAAAACCAGGAA
  GTCTCCACTGGGGTCAAGTGGGAAAACTATTTTGCAAGTACAGTGAACAGGGAGATGATGTGCTCTCCAG
  AGTTAAAAAACCTCATCCGTGCGGGCATTCCCCACGAGCACCGTTCCAAGGTGTGGAAGTGGTGTGTGGA
  CCGTCACACCAGGAAGTTCAAGGACAACACTGAGCCTGGCCACTTCCAGACCTTGCTGCAGAAGGCGCTG
  GAGAAACAGAACCCAGCCTCCAAGCAGATTGAGCTGGACTTGCTGCGAACTCTGCCCAACAACAAACATT
  ACTCCTGCCCCACCTCAGAAGGCATACAGAAGTTACGCAATGTCCTCCTCGCCTTCTCCTGGCGGAATCC
  AGATATCGGCTACTGTCAAGGCCTAAACAGGTTGGTGGCAGTGGCCCTCCTGTACCTGGAACAAGAAGAT
  GCTTTCTGGTGTCTCGTTACCATAGTGGAAGTTTTCATGCCTCGAGACTATTATACAAAGACTCTTTTAG
  GATCCCAGGTGGACCAGCGGGTGTTCAGAGACCTTATGAGTGAGAAGCTGCCTCGGTTGCATGGCCACTT
  TGAACAGTACAAAGTCGACTACACTCTCATCACTTTCAACTGGTTTCTGGTGGTATTTGTGGATAGTGTC
  GTTAGTGACATCCTCTTTAAAATATGGGACTCTTTCCTTTATGAAGGACCAAAGGTTATTTTCCGTTTTG
  CTCTGGCACTTTTTAAGTACAAGGAAGAGGAGATTTTGAAATTGCAAGATTCGATGTCTATATTTAAGTA
  TCTCCGCTACTTCACTCGCACTATCCTTGATGCTAGGAAGCTGATCAGTATCTCCTTTGGGGACCTGAAC
  CCTTTCCCCCTACGCCAGATCCGGAACCGACGCGCCTACCACTTGGAGAAAGTCCGGCTGGAGCTGACCG
  AGCTGGAGGCCATCCGTGAGGACTTCCTGCGTGAGCGGGACACCAGCCCTGACAAGGGTGAGCTGGTCAG
  TGACGAGGAGGAGGATACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226280 representing NM_144572
  Red=Cloning site Green=Tags(s)
  
  MPGAGARAEEGGGGGEGAAQGAAAEPGAGPAREPARLCGYLQKLSGKGPLRGYRSRWFVFDARRCYLYYF
  KSPQDALPLGHLDIADACFSYQGPDEAAEPGTEPPAHFQVHSAGAVTVLKAPNRQLMTYWLQELQQKRWE
  YCNSLDMVKWDSRTSPTPGDFPKGLVARDNTDLIYPHPNASAEKARNVLAVETVPGELVGEQAANQPAPG
  HPNSINFYSLKQWGNELKNSMSSFRPGRGHNDSRRTVFYTNEEWELLDPTPKDLEESIVQEEKKKLTPEG
  NKGVTGSGFPFDFGRNPYKGKRPLKDIIGSYKNRHSSGDPSSEGTSGSGSVSIRKPASEMQLQVQSQQEE
  LEQLKKDLSSQKELVRLLQQTVRSSQYDKYFTSSRLCEGVPKDTLELLHQKDDQILGLTSQLERFSLEKE
  SLQQEVRTLKSKVGELNEQLGMLMETIQAKDEVIIKLSEGEGNGPPPTVAPSSPSVVPVARDQLELDRLK
  DNLQGYKTQNKFLNKEILELSALRRNAERRERDLMAKYSSLEAKLCQIESKYLILLQEMKTPVCSEDQGP
  TREVIAQLLEDALQVESQEQPEQAFVKPHLVSEYDIYGFRTVPEDDEEEKLVAKVRALDLKTLYLTENQE
  VSTGVKWENYFASTVNREMMCSPELKNLIRAGIPHEHRSKVWKWCVDRHTRKFKDNTEPGHFQTLLQKAL
  EKQNPASKQIELDLLRTLPNNKHYSCPTSEGIQKLRNVLLAFSWRNPDIGYCQGLNRLVAVALLYLEQED
  AFWCLVTIVEVFMPRDYYTKTLLGSQVDQRVFRDLMSEKLPRLHGHFEQYKVDYTLITFNWFLVVFVDSV
  VSDILFKIWDSFLYEGPKVIFRFALALFKYKEEEILKLQDSMSIFKYLRYFTRTILDARKLISISFGDLN
  PFPLRQIRNRRAYHLEKVRLELTELEAIREDFLRERDTSPDKGELVSDEEEDT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144572
• ORF Size: 2889 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144572.1, NP_653173.1
• RefSeq ORF: 2892 bp
• Locus ID: 23102
• UniProt ID: Q9UPU7
• Cytogenetics: 15q24.3-q25.1
• Protein Families: Transcription Factors
• MW: 109.7 kDa
• Gene Summary: May act as a GTPase-activating protein.[UniProtKB/Swiss-Prot Function]