NELF (NSMF) (NM_001130970) Human Tagged ORF Clone

CAT#: RC225872

NSMF (Myc-DDK-tagged)-Human nasal embryonic LHRH factor (NELF), transcript variant 3

Product Images

Circular map for RC225872

• 

  Western blot validation of overexpression lysate (Cat# LY427332) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225872 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NELF
• Synonyms: HH9; NELF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225872 representing NM_001130970
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGCCGCCGCCTCCAGGAGGAGGGCGCTGAGGAGCGAGGCCATGTCCTCGGTGGCGGCCAAAGTGC
  GAGCAGCCCGAGCGTTTGGAGAGTACCTGTCCCAGAGTCACCCTGAGAACCGCAACGGCGCAGATCACCT
  GCTGGCTGATGCCTACTCTGGCCACGACGGGTCCCCCGAGATGCAGCCGGCCCCCCAGAACAAGCGCCGC
  CTGTCCCTCGTCTCCAACGGCTGCTACGAGGGCAGCCTCTCAGAGGAGCCCAGCATTAGGAAGCCCGCAG
  GCGAGGGCCCTCAGCCTCGAGTGTACACCATCTCTGGGGAGCCTGCCCTGCTGCCCAGCCCTGAGGCGGA
  GGCCATTGAGCTGGCGGTGGTGAAGGGGCGGCGGCAGCGGCACCCTCACCATCACAGCCAGCCCCTGCGC
  GCCAGCCCTGGTGGCAGCCGGGAGGACGTCAGCAGGCCCTGCCAGAGCTGGGCGGGCAGCCGCCAGGGCT
  CCAAGGAGTGCCCCGGATGTGCCCAGCTGGCTCCTGGCCCCACCCCTCGGGCCTTTGGGCTGGACCAGCC
  ACCTCTGCCTGAGACCTCCGGTCGCCGCAAGAAGCTGGAGAGGATGTACAGCGTTGACCGTGTGTCTGAC
  GACATCCCTATTCGTACCTGGTTCCCCAAGGAAAATCTTTTCAGCTTCCAGACAGCAACCACAACTATGC
  AAGCCATCTCGAACTTCCGCAAACACCTGCGCATGGTCGGCAGCCGGAGGGTGAAGGCCCAGACGTTCGC
  TGAGCGGCGCGAGCGGAGCTTCAGCCGGTCCTGGAGCGACCCCACCCCCATGAAAGCCGACACTTCCCAC
  GACTCCCGAGACAGCAGTGACCTGCAGAGCTCCCACTGCACGCTGGACGAGGCCTTCGAGGACCTGGACT
  GGGACACTGAGAAGGGCCTGGAGGCTGTGGCCTGCGACACCGAAGGCTTCGTGCCACCAAAGGTCATGCT
  CATTTCCTCCAAGGTGCCCAAGGCTGAGTACATCCCCACTATCATCCGCCGGGATGACCCCTCCATCATC
  CCCATCCTCTACGACCATGAGCACGCAACCTTCGAGGACATCCTTGAGGAGATAGAGAGGAAGCTGAACG
  TCTACCACAAGGGAGCCAAGATCTGGAAAATGCTGATTTTCTGCCAGGGAGGTCCTGGACACCTCTATCT
  CCTCAAGAACAAGGTGGCCACCTTTGCCAAAGTGGAGAAGGAAGAGGACATGATTCACTTCTGGAAGCGG
  CTGAGCCGCCTGATGAGCAAAGTGAACCCAGAGCCGAACGTCATCCACATCATGGGCTGCTACATTCTGG
  GGAACCCCAATGGAGAGAAGCTGTTCCAGAACCTCAGGACCCTCATGACTCCTTATAGGGTCACCTTCGA
  GTCACCCCTGGAGCTCTCAGCCCAAGGGAAGCAGATGATCGAGACGTACTTTGACTTCCGGTTGTATCGC
  CTGTGGAAGAGCCGCCAGCACTCGAAGCTGCTGGACTTTGACGACGTCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225872 representing NM_001130970
  Red=Cloning site Green=Tags(s)
  
  MGAAASRRRALRSEAMSSVAAKVRAARAFGEYLSQSHPENRNGADHLLADAYSGHDGSPEMQPAPQNKRR
  LSLVSNGCYEGSLSEEPSIRKPAGEGPQPRVYTISGEPALLPSPEAEAIELAVVKGRRQRHPHHHSQPLR
  ASPGGSREDVSRPCQSWAGSRQGSKECPGCAQLAPGPTPRAFGLDQPPLPETSGRRKKLERMYSVDRVSD
  DIPIRTWFPKENLFSFQTATTTMQAISNFRKHLRMVGSRRVKAQTFAERRERSFSRSWSDPTPMKADTSH
  DSRDSSDLQSSHCTLDEAFEDLDWDTEKGLEAVACDTEGFVPPKVMLISSKVPKAEYIPTIIRRDDPSII
  PILYDHEHATFEDILEEIERKLNVYHKGAKIWKMLIFCQGGPGHLYLLKNKVATFAKVEKEEDMIHFWKR
  LSRLMSKVNPEPNVIHIMGCYILGNPNGEKLFQNLRTLMTPYRVTFESPLELSAQGKQMIETYFDFRLYR
  LWKSRQHSKLLDFDDVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130970
• ORF Size: 1521 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130970.1, NP_001124442.1
• RefSeq ORF: 1524 bp
• Locus ID: 26012
• UniProt ID: Q6X4W1
• Cytogenetics: 9q34.3
• Protein Families: Druggable Genome
• MW: 57.2 kDa
• Gene Summary: The protein encoded by this gene is involved in guidance of olfactory axon projections and migration of luteinizing hormone-releasing hormone neurons. Defects in this gene are a cause of idiopathic hypogonadotropic hypogonadism (IHH). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]