SCRIBBLE (SCRIB) (NM_015356) Human Tagged ORF Clone
CAT#: RC224890
- TrueORF®
SCRIB (Myc-DDK-tagged)-Human scribbled homolog (Drosophila) (SCRIB), transcript variant 2
ORF Plasmid: tGFP
"NM_015356" in other vectors (2)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | SCRIBBLE |
Synonyms | CRIB1; SCRB1; SCRIB1; Vartul |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC224890 representing NM_015356
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCAAGTGCATCCCGCTGTGGCGCTGCAACCGGCACGTGGAGTCGGTGGACAAGCGGCACTGTTCGC TGCAGGCCGTGCCGGAGGAGATCTACCGCTACAGCCGCAGCCTGGAGGAGCTGCTGCTCGACGCCAACCA GCTGCGCGAGCTGCCCAAGCCTTTTTTCCGGCTGCTGAACTTGCGCAAGCTGGGCCTGAGCGACAACGAG ATCCAGCGGTTGCCTCCCGAGGTGGCCAACTTCATGCAGCTGGTGGAGCTGGACGTGTCCCGGAACGATA TCCCTGAGATCCCGGAGAGCATCAAGTTCTGCAAGGCTCTGGAGATCGCGGACTTCAGCGGGAACCCCCT CTCCAGGCTCCCTGATGGCTTCACTCAGCTGCGCAGCCTGGCTCACCTGGCCCTGAATGATGTGTCTCTG CAGGCACTGCCCGGGGACGTGGGCAACCTCGCCAACCTGGTGACCCTGGAGCTCCGGGAGAACCTGCTCA AGTCCCTGCCAGCGTCCCTGTCATTTCTGGTCAAGCTGGAACAGCTGGATCTGGGAGGCAACGATCTGGA AGTGCTGCCAGACACTCTGGGGGCTCTGCCCAATCTTCGGGAGCTGTGGCTTGACCGGAACCAGCTGTCA GCACTGCCCCCGGAGCTCGGGAACCTGCGGCGCCTGGTGTGCCTGGACGTGTCGGAAAACCGGCTGGAGG AGCTGCCTGCTGAGCTCGGCGGGCTGGTGCTGCTCACTGACCTGCTGCTGTCCCAGAACCTGCTGCGGAG GCTGCCCGACGGCATCGGTCAGCTGAAGCAGCTATCCATCCTAAAGGTAGACCAGAATCGGCTGTGCGAG GTGACTGAGGCCATCGGGGACTGTGAGAACCTCTCTGAGCTGATCCTCACGGAGAACCTGCTGATGGCCC TGCCCCGCTCCCTGGGAAAGCTGACTAAGCTGACCAACCTCAACGTGGACCGGAACCACCTCGAGGCGCT GCCGCCCGAGATCGGGGGCTGTGTGGCACTCAGCGTCCTCTCCTTGAGGGACAACCGCCTGGCCGTCCTG CCACCAGAGCTGGCCCACACGACAGAGCTGCACGTGCTGGACGTGGCGGGGAACCGCCTGCAGAGTCTGC CGTTCGCGCTCACCCACCTCAATCTCAAGGCCCTGTGGCTGGCAGAGAACCAGGCGCAGCCCATGCTCCG GTTCCAGACGGAGGATGATGCCCGGACCGGCGAGAAGGTGCTCACCTGCTACTTGCTGCCCCAGCAGCCC CCACCCAGCCTCGAGGATGCTGGGCAGCAGGGGAGCCTCTCGGAGACCTGGAGCGATGCCCCGCCGAGCC GCGTCAGCGTCATCCAGTTCCTGGAGGCCCCCATAGGTGATGAGGACGCTGAGGAAGCTGCAGCTGAGAA GCGGGGCCTACAGCGCCGGGCCACACCTCACCCCAGCGAGCTCAAGGTGATGAAGAGGAGCATCGAGGGG CGGCGGAGCGAGGCCTGCCCTTGCCAGCCAGACTCTGGGTCGCCCTTGCCTGCAGAGGAGGAGAAGCGGC TGAGTGCCGAGTCTGGCCTGAGTGAAGACTCTCGCCCATCTGCCAGCACAGTCTCTGAGGCTGAGCCCGA GGGCCCGTCGGCTGAGGCACAGGGTGGGAGCCAGCAGGAAGCCACGACTGCTGGCGGGGAGGAAGACGCC GAAGAGGACTACCAGGAGCCCACGGTGCATTTCGCAGAGGACGCACTGCTGCCCGGGGATGACAGGGAGA TCGAGGAGGGGCAGCCTGAGGCCCCCTGGACCCTGCCAGGCGGGAGGCAGCGGCTCATCCGCAAGGACAC ACCTCACTACAAAAAGCACTTCAAGATCTCCAAGCTGCCCCAGCCCGAGGCCGTTGTGGCTCTGCTGCAG GGCATGCAGCCTGATGGGGAGGGCCCTGTGGCTCCCGGGGGCTGGCACAATGGCCCCCACGCACCCTGGG CTCCTCGGGCCCAGAAGGAGGAGGAGGAGGAGGAAGAGGGTAGTCCTCAGGAGGAGGAGGAAGAGGAGGA GGAGGAAAACAGGGCTGAAGAGGAAGAGGCCAGCACTGAGGAGGAGGACAAGGAGGGGGCCGTGGTTTCT GCGCCCTCTGTCAAGGGAGTGTCGTTTGACCAGGCCAATAACCTGCTGATAGAGCCTGCTCGCATTGAGG AGGAAGAGCTGACCCTCACCATCCTGCGGCAGACTGGGGGCCTGGGCATCAGCATTGCGGGCGGCAAGGG CTCCACACCCTATAAGGGGGACGACGAGGGCATATTCATCTCTCGGGTGTCCGAGGAAGGCCCTGCGGCC CGGGCTGGAGTCCGTGTGGGTGACAAGCTCCTGGAGGTGAATGGTGTGGCTCTGCAGGGCGCCGAGCACC ACGAGGCCGTGGAGGCGCTCCGGGGGGCCGGCACTGCCGTGCAGATGCGAGTGTGGCGGGAGCGCATGGT GGAGCCTGAGAACGCGGTCACCATCACGCCGCTGCGGCCCGAGGATGATTACAGCCCCCGAGAGCGGCGG GGAGGGGGGCTGCGCCTGCCCCTGCTCCCGCCTGAGAGCCCCGGGCCCCTCCGTCAGCGCCACGTGGCCT GCCTGGCACGCAGCGAGAGGGGGCTGGGCTTCAGCATTGCTGGTGGGAAAGGCTCCACACCCTACAGGGC TGGTGATGCGGGCATCTTCGTCTCCCGCATTGCCGAGGGCGGTGCTGCTCACCGCGCGGGCACACTGCAG GTTGGCGACCGCGTCCTCTCTATTAATGGAGTGGACGTGACTGAGGCCAGGCATGACCACGCCGTCTCCC TGCTGACCGCTGCCTCCCCCACCATCGCCCTGCTGTTGGAGCGGGAGGCTGGGGGCCCTCTTCCTCCCAG CCCTCTGCCACATTCCTCACCCCCCACCGCTGCTGTTGCCACCACCAGCATAACCACTGCCACCCCCGGG GTGCCTGGGTTGCCGAGCCTGGCCCCCAGCCTGCTGGCTGCCGCGTTGGAAGGGCCATACCCAGTGGAGG AGATCCGTCTGCCAAGAGCTGGGGGCCCTCTGGGGCTTAGTATTGTCGGAGGCTCCGACCATTCCAGCCA CCCGTTTGGTGTCCAGGAGCCTGGTGTGTTCATCTCCAAGGTGCTCCCGCGGGGCCTGGCCGCTCGCAGC GGCCTGCGGGTTGGGGACCGCATCCTGGCAGTGAACGGGCAAGACGTGCGGGATGCCACGCACCAAGAAG CAGTCAGTGCCCTGCTCCGGCCCTGCCTGGAGCTGTCGCTGCTGGTGCGGAGGGACCCGGCACCCCCGGG CCTACGGGAACTGTGCATCCAGAAGGCACCTGGGGAGAGGCTGGGCATCAGCATCCGCGGGGGTGCCAGG GGCCACGCTGGCAACCCCCGCGACCCCACAGACGAGGGCATCTTCATCTCCAAGGTGAGCCCCACGGGGG CAGCCGGGCGCGACGGTCGGCTGCGTGTGGGTTTGCGGCTGTTGGAGGTGAACCAGCAGAGCCTGCTGGG CCTGACGCACGGCGAGGCGGTGCAGCTGCTCCGCAGTGTGGGCGACACCCTCACCGTGCTGGTCTGTGAC GGCTTCGAGGCCAGCACCGACGCAGCCCTGGAGGTGTCCCCAGGTGTCATTGCCAACCCCTTTGCGGCAG GCATCGGCCACCGGAACAGCCTGGAGAGCATCTCTTCCATCGACCGGGAGCTGAGCCCTGAGGGCCCAGG CAAGGAGAAGGAGCTGCCTGGACAGACCCTGCACTGGGGGCCCGAGGCCACAGAAGCCGCAGGTCGGGGT CTGCAGCCCCTGAAGCTGGACTACCGCGCCCTGGCCGCCGTGCCCAGCGCTGGCAGCGTGCAGAGGGTAC CGTCTGGAGCAGCTGGAGGGAAGATGGCTGAATCTCCCTGCTCCCCTAGTGGCCAGCAGCCGCCCTCCCC GCCTTCTCCGGATGAGCTGCCCGCCAATGTGAAGCAGGCCTACAGGGCCTTCGCGGCCGTGCCCACTTCT CACCCGCCTGAGGATGCCCCTGCCCAGCCCCCCACGCCTGGGCCTGCAGCCTCCCCGGAGCAGCTGTCCT TCCGGGAGCGGCAGAAGTACTTTGAGCTGGAGGTGCGCGTGCCCCAGGCCGAGGGCCCCCCTAAGCGCGT GTCCCTGGTGGGTGCTGACGACCTGCGGAAGATGCAGGAGGAGGAAGCCAGAAAACTACAGCAGAAGAGA GCGCAGATGCTGCGGGAGGCGGCAGAGGCTGGGGCCGAAGCGAGGCTCGCCCTGGACGGGGAGACGCTGG GCGAGGAGGAACAGGAGGATGAGCAGCCACCCTGGGCCAGCCCGAGCCCCACCTCAAGGCAGAGCCCGGC GTCCCCCCCGCCCCTGGGAGGTGGCGCCCCGGTGCGGACGGCCAAAGCTGAACGGCGCCACCAGGAGCGG CTGCGCGTGCAGAGTCCGGAGCCACCGGCACCCGAGCGTGCCCTGTCCCCTGCCGAGCTCCGGGCCCTGG AGGCCGAGAAGCGTGCGCTGTGGAGGGCAGCCAGGATGAAGTCATTGGAACAGGACGCTCTCCGAGCACA GATGGTCCTCAGCAGGTCCCAGGAAGGCCGGGGCACGCGGGGGCCCCTGGAGCGACTGGCCGAGGCCCCT TCCCCTGCGCCCACCCCGTCGCCCACCCCTGTGGAAGACCTCGGCCCCCAGACCAGCACCTCCCCGGGAC GCCTGTCACCGGACTTTGCTGAGGAGTTGAGGTCCCTGGAACCATCTCCCAGCCCTGGCCCGCAGGAGGA GGATGGAGAAGTGGCTCTGGTGCTTCTGGGCAGGCCCTCACCCGGCGCTGTGGGCCCTGAAGATGTGGCA CTGTGCAGCAGCCGCCGCCCCGTAAGGCCTGGGCGCCGTGGCCTGGGCCCTGTGCCCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224890 representing NM_015356
Red=Cloning site Green=Tags(s) MLKCIPLWRCNRHVESVDKRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNE IQRLPPEVANFMQLVELDVSRNDIPEIPESIKFCKALEIADFSGNPLSRLPDGFTQLRSLAHLALNDVSL QALPGDVGNLANLVTLELRENLLKSLPASLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLS ALPPELGNLRRLVCLDVSENRLEELPAELGGLVLLTDLLLSQNLLRRLPDGIGQLKQLSILKVDQNRLCE VTEAIGDCENLSELILTENLLMALPRSLGKLTKLTNLNVDRNHLEALPPEIGGCVALSVLSLRDNRLAVL PPELAHTTELHVLDVAGNRLQSLPFALTHLNLKALWLAENQAQPMLRFQTEDDARTGEKVLTCYLLPQQP PPSLEDAGQQGSLSETWSDAPPSRVSVIQFLEAPIGDEDAEEAAAEKRGLQRRATPHPSELKVMKRSIEG RRSEACPCQPDSGSPLPAEEEKRLSAESGLSEDSRPSASTVSEAEPEGPSAEAQGGSQQEATTAGGEEDA EEDYQEPTVHFAEDALLPGDDREIEEGQPEAPWTLPGGRQRLIRKDTPHYKKHFKISKLPQPEAVVALLQ GMQPDGEGPVAPGGWHNGPHAPWAPRAQKEEEEEEEGSPQEEEEEEEEENRAEEEEASTEEEDKEGAVVS APSVKGVSFDQANNLLIEPARIEEEELTLTILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAA RAGVRVGDKLLEVNGVALQGAEHHEAVEALRGAGTAVQMRVWRERMVEPENAVTITPLRPEDDYSPRERR GGGLRLPLLPPESPGPLRQRHVACLARSERGLGFSIAGGKGSTPYRAGDAGIFVSRIAEGGAAHRAGTLQ VGDRVLSINGVDVTEARHDHAVSLLTAASPTIALLLEREAGGPLPPSPLPHSSPPTAAVATTSITTATPG VPGLPSLAPSLLAAALEGPYPVEEIRLPRAGGPLGLSIVGGSDHSSHPFGVQEPGVFISKVLPRGLAARS GLRVGDRILAVNGQDVRDATHQEAVSALLRPCLELSLLVRRDPAPPGLRELCIQKAPGERLGISIRGGAR GHAGNPRDPTDEGIFISKVSPTGAAGRDGRLRVGLRLLEVNQQSLLGLTHGEAVQLLRSVGDTLTVLVCD GFEASTDAALEVSPGVIANPFAAGIGHRNSLESISSIDRELSPEGPGKEKELPGQTLHWGPEATEAAGRG LQPLKLDYRALAAVPSAGSVQRVPSGAAGGKMAESPCSPSGQQPPSPPSPDELPANVKQAYRAFAAVPTS HPPEDAPAQPPTPGPAASPEQLSFRERQKYFELEVRVPQAEGPPKRVSLVGADDLRKMQEEEARKLQQKR AQMLREAAEAGAEARLALDGETLGEEEQEDEQPPWASPSPTSRQSPASPPPLGGGAPVRTAKAERRHQER LRVQSPEPPAPERALSPAELRALEAEKRALWRAARMKSLEQDALRAQMVLSRSQEGRGTRGPLERLAEAP SPAPTPSPTPVEDLGPQTSTSPGRLSPDFAEELRSLEPSPSPGPQEEDGEVALVLLGRPSPGAVGPEDVA LCSSRRPVRPGRRGLGPVPS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_015356 |
ORF Size | 4890 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015356.4, NP_056171.3 |
RefSeq Size | 5201 bp |
RefSeq ORF | 4893 bp |
Locus ID | 23513 |
UniProt ID | Q14160 |
Cytogenetics | 8q24.3 |
MW | 174.9 kDa |
Gene Summary | This gene encodes a protein that was identified as being similar to the Drosophila scribble protein. The mammalian protein is involved in tumor suppression pathways. As a scaffold protein involved in cell polarization processes, this protein binds to many other proteins. The encoded protein binds to papillomavirus E6 protein via its PDZ domain and the C-terminus of E6. Two alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Nov 2011] |
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