GPR64 (ADGRG2) (NM_001079858) Human Tagged ORF Clone

CAT#: RC224530

GPR64 (Myc-DDK-tagged)-Human G protein-coupled receptor 64 (GPR64), transcript variant 1

Product Images

Circular map for RC224530

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPR64
• Synonyms: CBAVDX; EDDM6; GPR64; HE6; TM7LN2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224530 representing NM_001079858
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTTCTCTGTCAGGCAGTGTGGCCATGTTGGCAGAACTGAAGAAGTTTTACTGACGTTCAAGATAT
  TCCTTGTCATCATTTGTCTTCATGTCGTTCTGGTAACATCCCTGGAAGAAGATACTGATAATTCCAGTTT
  GTCACCACCACCTGCTAAATTATCTGTTGTCAGTTTTGCCCCCTCCTCCAATGGTACTCCAGAGGTTGAA
  ACAACAAGCCTCAATGATGTTACTTTAAGCTTACTCCCTTCAAACGAAACAGAAAAAACTAAAATCACTA
  TAGTAAAAACCTTCAATGCATCAGGCGTCAAACCCCAGAGAAATATCTGCAATTTGTCATCTATTTGCAA
  TGACTCAGCATTTTTTAGAGGTGAGATCATGTTTCAATATGATAAAGAAAGCACTGTTCCCCAGAATCAA
  CATATAACGAATGGCACCTTAACTGGAGTCCTGTCTCTAAGTGAATTAAAACGCTCAGAGCTCAACAAAA
  CCCTGCAAACCCTAAGTGAGACTTACTTTATAATGTGTGCTACAGCAGAGGCCCAAAGCACATTAAATTG
  TACATTCACAATAAAACTGAATAATACAATGAATGCATGTGCTGTAATAGCTGCTTTGGAAAGAGTAAAG
  ATTCGACCAATGGAACACTGCTGCTGTTCTGTCAGGATACCCTGCCCTTCCTCCCCAGAAGAGTTGGAAA
  AGCTTCAGTGTGACCTGCAGGATCCCATTGTCTGTCTTGCTGACCATCCACGTGGCCCACCATTTTCTTC
  CAGCCAATCCATCCCAGTGGTGCCTCGGGCCACTGTGCTTTCCCAGGTCCCCAAAGCTACCTCTTTTGCT
  GAGCCTCCAGATTATTCACCTGTGACCCACAATGTTCCCTCTCCAATAGGGGAGATTCAACCCCTTTCAC
  CCCAGCCTTCAGCTCCCATAGCTTCCAGCCCTGCCATTGACATGCCCCCACAGTCTGAAACGATCTCTTC
  CCCTATGCCCCAAACCCATGTCTCCGGCACCCCACCTCCTGTGAAAGCCTCATTTTCCTCTCCCACCGTG
  TCTGCCCCTGCGAATGTCAACACTACCAGCGCACCTCCTGTCCAGACAGACATCGTCAACACCAGCAGTA
  TTTCTGATCTTGAGAACCAAGTGTTGCAGATGGAGAAGGCTCTGTCCTTGGGCAGCCTGGAGCCTAACCT
  CGCAGGAGAAATGATCAACCAAGTCAGCAGACTCCTTCATTCCCCGCCTGACATGCTGGCCCCTCTGGCT
  CAAAGATTGCTGAAAGTAGTGGATGACATTGGCCTACAGCTGAACTTTTCAAACACGACTATAAGTCTAA
  CCTCCCCTTCTTTGGCTCTGGCTGTGATCAGAGTGAATGCCAGTAGTTTCAACACAACTACCTTTGTGGC
  CCAAGACCCTGCAAATCTTCAGGTTTCTCTGGAAACCCAAGCTCCTGAGAACAGTATTGGCACAATTACT
  CTTCCTTCATCGCTGATGAATAATTTACCAGCTCATGACATGGAGCTAGCTTCCAGGGTTCAGTTCAATT
  TTTTTGAAACACCTGCTTTGTTTCAGGATCCTTCCCTGGAGAACCTCTCTCTGATCAGCTACGTCATATC
  ATCGAGTGTTGCAAACCTGACCGTCAGGAACTTGACAAGAAACGTGACAGTCACATTAAAGCACATCAAC
  CCGAGCCAGGATGAGTTAACAGTGAGATGTGTATTTTGGGACTTGGGCAGAAATGGTGGCAGAGGAGGCT
  GGTCAGACAATGGCTGCTCTGTCAAAGACAGGAGATTGAATGAAACCATCTGTACCTGTAGCCATCTAAC
  AAGCTTCGGCGTTCTGCTGGACCTATCTAGGACATCTGTGCTGCCTGCTCAAATGATGGCTCTGACGTTC
  ATTACATATATTGGTTGTGGGCTTTCATCAATTTTTCTGTCAGTGACTCTTGTAACCTACATAGCTTTTG
  AAAAGATCCGGAGGGATTACCCTTCCAAAATCCTCATCCAGCTGTGTGCTGCTCTGCTTCTGCTGAACCT
  GGTCTTCCTCCTGGACTCGTGGATTGCTCTGTATAAGATGCAAGGCCTCTGCATCTCAGTGGCTGTATTT
  CTTCATTATTTTCTCTTGGTCTCATTCACATGGATGGGCCTAGAAGCATTCCATATGTACCTGGCCCTTG
  TCAAAGTATTTAATACTTACATCCGAAAATACATCCTTAAATTCTGCATTGTCGGTTGGGGGGTACCAGC
  TGTGGTTGTGACCATCATCCTGACTATATCCCCAGATAACTATGGGCTTGGATCCTATGGGAAATTCCCC
  AATGGTTCACCGGATGACTTCTGCTGGATCAACAACAATGCAGTATTCTACATTACGGTGGTGGGATATT
  TCTGTGTGATATTTTTGCTGAACGTCAGCATGTTCATTGTGGTCCTGGTTCAGCTCTGTCGAATTAAAAA
  GAAGAAGCAACTGGGAGCCCAGCGAAAAACCAGTATTCAAGACCTCAGGAGTATCGCTGGCCTTACATTT
  TTACTGGGAATAACTTGGGGCTTTGCCTTCTTTGCCTGGGGACCAGTTAACGTGACCTTCATGTATCTGT
  TTGCCATCTTTAATACCTTACAAGGATTTTTCATATTCATCTTTTACTGTGTGGCCAAAGAAAATGTCAG
  GAAGCAATGGAGGCGGTATCTTTGTTGTGGAAAGTTACGGCTGGCTGAAAATTCTGACTGGAGTAAAACT
  GCTACTAATGGTTTAAAGAAGCAGACTGTAAACCAAGGAGTGTCCAGCTCTTCAAATTCCTTACAGTCAA
  GCAGTAACTCCACTAACTCCACCACACTGCTAGTGAATAATGATTGCTCAGTACACGCAAGCGGGAATGG
  AAATGCTTCTACAGAGAGGAATGGGGTCTCTTTTAGTGTTCAGAATGGAGATGTGTGCCTTCACGATTTC
  ACTGGAAAACAGCACATGTTTAACGAGAAGGAAGATTCCTGCAATGGGAAAGGCCGTATGGCTCTCAGAA
  GGACTTCAAAGCGGGGAAGCTTACACTTTATTGAGCAAATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224530 representing NM_001079858
  Red=Cloning site Green=Tags(s)
  
  MVFSVRQCGHVGRTEEVLLTFKIFLVIICLHVVLVTSLEEDTDNSSLSPPPAKLSVVSFAPSSNGTPEVE
  TTSLNDVTLSLLPSNETEKTKITIVKTFNASGVKPQRNICNLSSICNDSAFFRGEIMFQYDKESTVPQNQ
  HITNGTLTGVLSLSELKRSELNKTLQTLSETYFIMCATAEAQSTLNCTFTIKLNNTMNACAVIAALERVK
  IRPMEHCCCSVRIPCPSSPEELEKLQCDLQDPIVCLADHPRGPPFSSSQSIPVVPRATVLSQVPKATSFA
  EPPDYSPVTHNVPSPIGEIQPLSPQPSAPIASSPAIDMPPQSETISSPMPQTHVSGTPPPVKASFSSPTV
  SAPANVNTTSAPPVQTDIVNTSSISDLENQVLQMEKALSLGSLEPNLAGEMINQVSRLLHSPPDMLAPLA
  QRLLKVVDDIGLQLNFSNTTISLTSPSLALAVIRVNASSFNTTTFVAQDPANLQVSLETQAPENSIGTIT
  LPSSLMNNLPAHDMELASRVQFNFFETPALFQDPSLENLSLISYVISSSVANLTVRNLTRNVTVTLKHIN
  PSQDELTVRCVFWDLGRNGGRGGWSDNGCSVKDRRLNETICTCSHLTSFGVLLDLSRTSVLPAQMMALTF
  ITYIGCGLSSIFLSVTLVTYIAFEKIRRDYPSKILIQLCAALLLLNLVFLLDSWIALYKMQGLCISVAVF
  LHYFLLVSFTWMGLEAFHMYLALVKVFNTYIRKYILKFCIVGWGVPAVVVTIILTISPDNYGLGSYGKFP
  NGSPDDFCWINNNAVFYITVVGYFCVIFLLNVSMFIVVLVQLCRIKKKKQLGAQRKTSIQDLRSIAGLTF
  LLGITWGFAFFAWGPVNVTFMYLFAIFNTLQGFFIFIFYCVAKENVRKQWRRYLCCGKLRLAENSDWSKT
  ATNGLKKQTVNQGVSSSSNSLQSSSNSTNSTTLLVNNDCSVHASGNGNASTERNGVSFSVQNGDVCLHDF
  TGKQHMFNEKEDSCNGKGRMALRRTSKRGSLHFIEQM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001079858
• ORF Size: 3051 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001079858.3
• RefSeq Size: 4895 bp
• RefSeq ORF: 3054 bp
• Locus ID: 10149
• UniProt ID: Q8IZP9
• Cytogenetics: Xp22.13
• Protein Families: Druggable Genome, GPCR, Transmembrane
• MW: 111.6 kDa
• Gene Summary: This gene encodes a member of the G protein-coupled receptor family described as an epididymis-specific transmembrane protein. The encoded protein may be proteolytically processed as it contains a motif shown to be a protein scission motif in some members of this family (PMID: 11973329). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]