MICALCL (NM_032867) Human Tagged ORF Clone

CAT#: RC224524

MICALCL (Myc-DDK-tagged)-Human MICAL C-terminal like (MICALCL)

Product Images

Circular map for RC224524

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  Western blot validation of overexpression lysate (Cat# LY409900) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224524 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MICALCL protein (Cat# TP324524). The protein was produced from HEK293T cells transfected with MICALCL cDNA clone (Cat# RC224524) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MICALCL
• Synonyms: Ebitein1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224524 representing NM_032867
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACCTCCTAAGGACCCTTCTCCTTCTCTTCCTCTTCCTTCATCGTCTTCCCATTCATCTTCCCCAC
  CATCTTCTTCTTCAACCAGTGTTTCTGGGAATGCTCCAGATGGTTCCTCCCCGCCTCAGATGACAGCTTC
  TGAGCCCCTCTCGCAAGTCTCGAGAGGTCATCCAAGTCCTCCCACCCCAAACTTTCGGAGGCGAGCCGTA
  GCCCAAGGAGCACCCAGGGAAATTCCCCTGTATCTGCCTCATCACCCAAAGCCAGAGTGGGCAGAGTACT
  GCCTGGTGAGCCCTGGTGAAGATGGCCTCTCAGACCCTGCAGAGATGACTTCTGATGAGTGCCAGCCAGC
  AGAGGCCCCTCTTGGGGACATCGGAAGCAACCACAGAGACCCACACCCCATCTGGGGGAAGGACAGGAGC
  TGGACAGGGCAAGAGCTATCTCCCTTGGCTGGAGAAGACCGGGAAAAAGGGAGTACTGGAGCCAGGAAGG
  AAGAAGAGGGAGGGCCAGTGCTGGTAAAGGAGAAGTTGGGCCTGAAGAAGTTAGTCCTCACTCAGGAGCA
  GAAGACCATGTTGTTGGATTGGAATGACTCCATCCCTGAGAGTGTGCACCTCAAAGCTGGGGAGCGAATT
  TCCCAGAAAAGTGCTGAGAATGGTAGAGGAGGCCGTGTGCTAAAACCAGTCCGCCCCCTGCTGCTCCCTA
  GGGCAGCAGGAGAGCCCCTGCCAACCCAGAGAGGGGCCCAGGAGAAGATGGGGACCCCTGCGGAACAAGC
  TCAAGGGGAGCGAAACGTGCCTCCACCCAAGTCCCCACTGCGGCTCATAGCCAATGCCATCCGAAGGTCT
  CTAGAGCCCCTCCTTTCCAACTCTGAAGGCGGGAAGAAGGCCTGGGCCAAGCAAGAATCCAAAACTTTGC
  CCGCACAGGCCTGCACTCGCTCATTCAGCCTTCGGAAAACCAATTCCAATAAAGACGGGGACCAGCATTC
  CCCTGGGAGAAACCAGTCCTCAGCCTTTAGCCCTCCTGACCCTGCCCTCCGCACCCACAGTTTGCCCAAT
  CGGCCATCCAAGGTCTTTCCTGCACTTAGGTCCCCACCCTGCAGCAAGATTGAAGATGTCCCCACACTCC
  TCGAGAAAGTGAGTTTGCAAGAGAACTTCCCAGATGCTTCTAAGCCTCCAAAGAAAAGAATCTCACTTTT
  TTCCTCCCTCAGACTCAAAGACAAATCTTTTGAGAGTTTCCTCCAAGAATCCAGACAAAGAAAGGACATC
  AGGGACCTCTTTGGCAGCCCCAAGAGGAAGGTGCTGCCTGAAGATAGTGCGCAGGCCCTGGAGAAGCTGC
  TGCAGCCTTTCAAAAGCACCTCCCTGCGCCAGGCAGCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCC
  TCCTCCTCCTACAGCGGGAGGTGCAGACTCCAAGAACTTTCCCCTCAGAGCACAGGTAACAGAGGCTTCC
  TCTTCTGCCTCTTCAACCTCCTCCTCCTCTGCAGATGAAGAATTTGATCCCCAGCTTTCCTTGCAGTTAA
  AGGAGAAGAAGACACTTAGAAGAAGAAAGAAGCTAGAAAAAGCAATGAAGCAGTTGGTTAAGCAAGAAGA
  ATTGAAAAGACTCTATAAGGCTCAGGCCATCCAGAGGCAGCTGGAGGAGGTGGAGGAGCGGCAGAGGGCT
  TCTGAGATCCAGGGTGTGAGGCTGGAGAAGGCGTTGCGAGGAGAAGCAGATTCAGGCACACAGGATGAAG
  CACAGCTTTTGCAGGAATGGTTTAAGCTGGTTCTGGAGAAGAATAAATTAATGCGATATGAGTCGGAGCT
  CCTAATCATGGCCCAGGAACTGGAATTAGAAGATCATCAAAGCAGACTGGAGCAGAAACTGAGAGAGAAA
  ATGCTCAAGGAGGAGAGCCAGAAAGATGAGAAGGATCTAAACGAAGAGCAAGAAGTATTCACCGAGCTGA
  TGCAAGTGATTGAGCAAAGGGACAAACTCGTCGATTCCTTAGAGGAACAACGCATCAGAGAAAAAGCCGA
  GGACCAGCACTTTGAAAGCTTCGTATTCTCCAGAGGCTGTCAGCTGAGCAGGACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224524 representing NM_032867
  Red=Cloning site Green=Tags(s)
  
  MSPPKDPSPSLPLPSSSSHSSSPPSSSSTSVSGNAPDGSSPPQMTASEPLSQVSRGHPSPPTPNFRRRAV
  AQGAPREIPLYLPHHPKPEWAEYCLVSPGEDGLSDPAEMTSDECQPAEAPLGDIGSNHRDPHPIWGKDRS
  WTGQELSPLAGEDREKGSTGARKEEEGGPVLVKEKLGLKKLVLTQEQKTMLLDWNDSIPESVHLKAGERI
  SQKSAENGRGGRVLKPVRPLLLPRAAGEPLPTQRGAQEKMGTPAEQAQGERNVPPPKSPLRLIANAIRRS
  LEPLLSNSEGGKKAWAKQESKTLPAQACTRSFSLRKTNSNKDGDQHSPGRNQSSAFSPPDPALRTHSLPN
  RPSKVFPALRSPPCSKIEDVPTLLEKVSLQENFPDASKPPKKRISLFSSLRLKDKSFESFLQESRQRKDI
  RDLFGSPKRKVLPEDSAQALEKLLQPFKSTSLRQAAPPPPPPPPPPPPPPTAGGADSKNFPLRAQVTEAS
  SSASSTSSSSADEEFDPQLSLQLKEKKTLRRRKKLEKAMKQLVKQEELKRLYKAQAIQRQLEEVEERQRA
  SEIQGVRLEKALRGEADSGTQDEAQLLQEWFKLVLEKNKLMRYESELLIMAQELELEDHQSRLEQKLREK
  MLKEESQKDEKDLNEEQEVFTELMQVIEQRDKLVDSLEEQRIREKAEDQHFESFVFSRGCQLSRT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032867
• ORF Size: 2085 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032867.3
• RefSeq Size: 3044 bp
• RefSeq ORF: 2088 bp
• Locus ID: 84953
• UniProt ID: Q6ZW33
• Cytogenetics: 11p15.3
• Protein Families: Protease
• MW: 77.1 kDa
• Gene Summary: May cooperate with MAPK1/ERK2 via an intracellular signal transduction pathway in the morphogenetic development of round spermatids to spermatozoa. May act as Rab effector protein and play a role in vesicle trafficking.[UniProtKB/Swiss-Prot Function]