KIAA1486 (NYAP2) (NM_020864) Human Tagged ORF Clone

CAT#: RC224448

NYAP2 (Myc-DDK-tagged)-Human KIAA1486 (KIAA1486)

Product Images

Circular map for RC224448

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KIAA1486
• Synonyms: KIAA1486
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224448 representing NM_020864
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATATCCTCCAAGATGATGAGTTCTAATCCTGAGGAAGACCCTTTGGACACATTTCTCCAGTACATTG
  AGGATATGGGGATGAAGGCCTATGATGGCTTGGTTATTCAGAATGCGTCAGATATTGCTCGAGAGAATGA
  TCGCTTGAGAAATGAAACTAACCTAGCCTATTTGAAAGAGAAGAATGAAAAACGCCGAAGACAAGAAGAA
  GCAATAAAGCGCATAGGTGGAGAAGTAGGGCGAGGCCACGAAGGAAGTTACGTGGGCAAACATTTCCGCA
  TGGGATTCATGACAATGCCTGCTCCTCAGGACAGACTTCCCCATCCTTGCTCCAGTGGCTTTTCTGTGAG
  ATCACAGTCCCTGCACTCGGTTGGGGGCACAGACGATGACAGCAGCTGTGGCTCACGGAGACAACCACCA
  CCCAAACCCAAGAGGGACCCCAGCACCAAGCTGAGCACCTCATCAGAGACAGTCAGCAGCACTGCAGCCA
  GTAAGAGCGGGAAAACCCCTGAGAGGACTGAAGCGTCAGCTAAACCAAGACCCCACAGCGATGAATATTC
  CAAGAAGATTCCTCCTCCCAAACCGAAGCGAAATCCGAACACTCAGCTGAGCACATCTTTCGATGAAACG
  TACATCAAAAAGCATGGGCCCCGGAGGACGTCGCTGCCGCGGGACTCCTCCTTGTCCCAGATGGGCAGCC
  CCGCGGGAGACCCCGAGGAAGAGGAGCCCGTGTACATCGAGATGGTGGGGAACATTCTCAGAGACTTCAG
  GAAGGAGGACGATGACCAGAGCGAGGCCGTCTACGAGGAAATGAAGTACCCTATCTTTGACGACTTGGGC
  CAAGACGCCAAATGTGACTTCGACCATCACAGCTGTTCTTCGCAGTGTGCTACTCCCACGGTGCCTGACT
  TGGACTTCGCCAAGGCCTCAGTGCCATGCCCCCCCAAGGGGCTGCTTTGCGACATCCCTCCGCCCTTCCC
  CAACCTGCTTTCTCACAGACCCCCGCTGCTGGTATTTCCCCCCGCCCCCGTGCATTGCTCCCCCAACTCC
  GACGAGTCCCCGCTTACCCCTCTGGAGGTCACGAAGCTTCCCGTGCTGGAAAACGTGTCTTACATGAAAC
  AGCCAGCCGGGGCGTCGCCCTCCACGCTGCCGTCCCACGTCCCCGGCCATGCGAAACTGGAGAAAGAGCA
  GGCCGCGGCCCTGGGACCTGCCTCTGCCACCCCTGCGCTCTCCTCGTCGCCCCCACCCCCGTCTACGCTG
  TACCGAACCCAGTCTCCCCATGGCTACCCTAAAAGTCACTCCACCTCTCCCTCCCCCGTCAGCATGGGGA
  GGTCCCTGACTCCCCTGAGCCTCAAAAGGCCTCCCCCTTACGACGCTGTGCATTCGGGCAGCCTCTCAAG
  GAGCTCTCCTTCAGTGCCTCACTCGACCCCCAGACCCGTGTCGCAAGATGGGGCCAAGATGGTCAACGCC
  GCGGTGAACACCTACGGGGCAGCCCCGGGTGGCTCCCGGTCCCGGACACCCACGAGCCCGCTGGAGGAGC
  TGACCAGCCTCTTCTCCTCCGGCCGCAGCCTGCTGCGCAAGTCGTCCAGTGGCCGGCGCTCCAAAGAGCC
  TGCAGAGAAATCAACAGAGGAACTGAAAGTCCGAAGCCACAGCACGGAGCCATTACCAAAGTTGGACAAC
  AAGGAAAGAGGCCACCATGGGGCGTCTTCCTCCAGAGAGCCTGTCAAAGCTCAGGAATGGGATGGAACAC
  CAGGGACACCTGTGGTCACCAGTCGACTAGGAAGATGCTCTGTGAGCCCCACCTTGTTAGCGGGAAACCA
  CAGTTCAGAGCCTAAAGTAAGCTGCAAATTAGGCCGGTCTGCGTCGACGTCAGGTGTGCCTCCTCCATCA
  GTCACTCCCCTCAGGCAAAGCAGTGACCTGCAACAGAGCCAGGTACCATCATCGTTAGCCAATCGTGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224448 representing NM_020864
  Red=Cloning site Green=Tags(s)
  
  MISSKMMSSNPEEDPLDTFLQYIEDMGMKAYDGLVIQNASDIARENDRLRNETNLAYLKEKNEKRRRQEE
  AIKRIGGEVGRGHEGSYVGKHFRMGFMTMPAPQDRLPHPCSSGFSVRSQSLHSVGGTDDDSSCGSRRQPP
  PKPKRDPSTKLSTSSETVSSTAASKSGKTPERTEASAKPRPHSDEYSKKIPPPKPKRNPNTQLSTSFDET
  YIKKHGPRRTSLPRDSSLSQMGSPAGDPEEEEPVYIEMVGNILRDFRKEDDDQSEAVYEEMKYPIFDDLG
  QDAKCDFDHHSCSSQCATPTVPDLDFAKASVPCPPKGLLCDIPPPFPNLLSHRPPLLVFPPAPVHCSPNS
  DESPLTPLEVTKLPVLENVSYMKQPAGASPSTLPSHVPGHAKLEKEQAAALGPASATPALSSSPPPPSTL
  YRTQSPHGYPKSHSTSPSPVSMGRSLTPLSLKRPPPYDAVHSGSLSRSSPSVPHSTPRPVSQDGAKMVNA
  AVNTYGAAPGGSRSRTPTSPLEELTSLFSSGRSLLRKSSSGRRSKEPAEKSTEELKVRSHSTEPLPKLDN
  KERGHHGASSSREPVKAQEWDGTPGTPVVTSRLGRCSVSPTLLAGNHSSEPKVSCKLGRSASTSGVPPPS
  VTPLRQSSDLQQSQVPSSLANRD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020864
• ORF Size: 1959 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020864.1, NP_065915.1
• RefSeq Size: 4590 bp
• RefSeq ORF: 1962 bp
• Locus ID: 57624
• UniProt ID: Q9P242
• Cytogenetics: 2q36.3
• MW: 70.4 kDa
• Gene Summary: Activates PI3K and concomitantly recruits the WAVE1 complex to the close vicinity of PI3K and regulates neuronal morphogenesis.[UniProtKB/Swiss-Prot Function]