Dynamin 3 (DNM3) (NM_015569) Human Tagged ORF Clone
CAT#: RC224326
DNM3 (Myc-DDK-tagged)-Human dynamin 3 (DNM3), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_015569" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Dynamin 3 |
Synonyms | Dyna III |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC224326 representing NM_015569.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGGAACCGGGAGATGGAGGAGCTGATCCCGCTGGTGAACCGTCTGCAGGACGCGTTTTCGGCGCTG GGACAGAGCTGCCTGCTGGAGCTGCCGCAGATCGCCGTGGTGGGCGGCCAGAGCGCCGGCAAGAGCTCG GTGCTCGAGAACTTCGTGGGCAGGGACTTTCTCCCTCGAGGGTCGGGCATTGTAACAAGACGACCTCTT GTGCTGCAGCTTGTTACTTCTAAAGCAGAATATGCCGAGTTTCTACATTGCAAAGGAAAGAAATTTACA GATTTTGATGAAGTTCGCCTTGAGATTGAAGCAGAAACAGATCGCGTGACTGGAATGAATAAAGGCATT TCCTCCATACCCATTAATTTACGAGTCTATTCCCCACACGTGTTAAATCTAACCCTTATTGATCTACCT GGAATAACTAAAGTGCCTGTGGGAGATCAGCCACCAGATATCGAGTATCAGATCAGAGAAATGATTATG CAGTTCATCACGAGGGAGAACTGTCTGATTTTAGCTGTTACTCCAGCCAACACTGATCTTGCAAACTCA GATGCGCTGAAGCTAGCTAAAGAAGTTGATCCTCAAGGTCTGAGAACCATTGGAGTTATCACCAAACTG GACCTTATGGATGAAGGAACGGATGCCAGGGATGTTCTAGAGAACAAACTGTTGCCTCTTCGCAGGGGT TACGTGGGGGTGGTAAACAGAAGCCAGAAGGACATAGATGGGAAGAAGGACATAAAGGCAGCCATGCTG GCAGAGAGGAAGTTTTTCCTTTCCCACCCGGCTTACAGACATATCGCTGACCGAATGGGAACCCCACAC CTGCAGAAGGTCCTTAATCAGCAACTTACCAACCACATTCGGGATACCCTACCAAACTTCAGGAACAAA CTACAGGGACAGTTGCTCTCCATAGAACATGAAGTAGAAGCCTACAAAAATTTCAAACCAGAAGACCCA ACAAGGAAGACCAAAGCATTGCTGCAGATGGTTCAGCAATTTGCTGTGGACTTTGAGAAGAGAATTGAA GGGTCAGGGGATCAAGTAGATACCCTGGAACTCTCAGGTGGTGCTAAAATCAATCGTATTTTTCATGAA CGCTTTCCTTTTGAGATAGTAAAGATGGAGTTCAATGAGAAAGAATTGCGAAGAGAAATAAGCTATGCA ATCAAAAACATACATGGTATCAGGACAGGGTTGTTTACTCCAGACATGGCATTTGAAGCGATAGTCAAG AAACAGATTGTAAAGTTGAAAGGGCCTTCCTTGAAGAGTGTGGATCTGGTAATACAAGAATTAATCAAC ACTGTGAAGAAGTGTACCAAAAAACTGGCAAACTTCCCCAGACTCTGCGAGGAAACGGAAAGGATTGTT GCTAACCACATTCGTGAGCGAGAAGGGAAGACAAAGGACCAGGTATTGCTATTGATTGACATTCAAGTC TCTTACATCAACACCAACCATGAAGACTTCATTGGCTTCGCAAATGCTCAGCAGAGGAGCAGTCAGGTT CACAAGAAAACCACAGTTGGAAATCAGGTGATTCGCAAGGGGTGGCTCACCATCAGCAACATTGGCATC ATGAAAGGCGGCTCGAAGGGATACTGGTTCGTCCTTACTGCGGAAAGCTTGTCCTGGTATAAAGATGAT GAGGAAAAAGAAAAGAAGTACATGCTTCCCTTGGACAACCTGAAAGTTCGGGATGTGGAAAAGAGCTTT ATGTCTAGCAAGCACATCTTTGCACTCTTTAATACAGAGCAAAGGAATGTATACAAAGACTATCGCTTC CTTGAGCTGGCATGTGATTCCCAGGAGGATGTCGACAGCTGGAAGGCATCTCTACTAAGAGCTGGGGTC TATCCTGACAAATCTGTAGGGAACAACAAAGCTGAAAATGATGAGAATGGACAAGCAGAAAACTTTTCC ATGGACCCACAATTGGAGAGGCAAGTGGAGACCATTCGCAACCTCGTAGACTCCTACATGTCCATTATC AACAAATGTATCCGAGATCTAATTCCAAAAACAATAATGCACCTTATGATCAATAACGTTAAAGATTTC ATAAATTCCGAGCTCCTAGCACAGTTGTATTCTTCAGAGGACCAAAATACCCTGATGGAGGAATCTGCT GAGCAGGCTCAGCGCCGGGATGAGATGCTTCGAATGTATCAAGCACTGAAAGAAGCCCTTGGGATAATT GGGGACATCAGCACGGCCACCGTGTCCACTCCGGCACCCCCTCCAGTGGATGACTCCTGGATACAGCAC TCTCGCAGGTCACCTCCTCCAAGCCCCACAACCCAAAGGAGGCCAACACTAAGTGCTCCCCTCGCAAGG CCCACATCCGGCCGAGGACCAGCTCCTGCCATTCCCTCTCCTGGCCCCCACTCTGGGGCTCCTCCAGTC CCATTCCGTCCAGGCCCATTACCTCCTTTCCCCAGCAGCAGTGACTCCTTCGGAGCCCCTCCACAAGTT CCATCTAGGCCTACGAGGGCCCCGCCCAGTGTCCCAAGCCGGAGACCACCCCCATCACCAACTCGTCCC ACTATAATCCGCCCACTAGAATCCTCCCTGTTAGAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA >Peptide sequence encoded by RC224326
Blue=ORF Red=Cloning site Green=Tag(s) MGNREMEELIPLVNRLQDAFSALGQSCLLELPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPL VLQLVTSKAEYAEFLHCKGKKFTDFDEVRLEIEAETDRVTGMNKGISSIPINLRVYSPHVLNLTLIDLP GITKVPVGDQPPDIEYQIREMIMQFITRENCLILAVTPANTDLANSDALKLAKEVDPQGLRTIGVITKL DLMDEGTDARDVLENKLLPLRRGYVGVVNRSQKDIDGKKDIKAAMLAERKFFLSHPAYRHIADRMGTPH LQKVLNQQLTNHIRDTLPNFRNKLQGQLLSIEHEVEAYKNFKPEDPTRKTKALLQMVQQFAVDFEKRIE GSGDQVDTLELSGGAKINRIFHERFPFEIVKMEFNEKELRREISYAIKNIHGIRTGLFTPDMAFEAIVK KQIVKLKGPSLKSVDLVIQELINTVKKCTKKLANFPRLCEETERIVANHIREREGKTKDQVLLLIDIQV SYINTNHEDFIGFANAQQRSSQVHKKTTVGNQVIRKGWLTISNIGIMKGGSKGYWFVLTAESLSWYKDD EEKEKKYMLPLDNLKVRDVEKSFMSSKHIFALFNTEQRNVYKDYRFLELACDSQEDVDSWKASLLRAGV YPDKSVGNNKAENDENGQAENFSMDPQLERQVETIRNLVDSYMSIINKCIRDLIPKTIMHLMINNVKDF INSELLAQLYSSEDQNTLMEESAEQAQRRDEMLRMYQALKEALGIIGDISTATVSTPAPPPVDDSWIQH SRRSPPPSPTTQRRPTLSAPLARPTSGRGPAPAIPSPGPHSGAPPVPFRPGPLPPFPSSSDSFGAPPQV PSRPTRAPPSVPSRRPPPSPTRPTIIRPLESSLLD SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV Recombinant protein using RC224326 also available, TP324326 |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_015569 |
ORF Size | 2589 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq Size | 7644 bp |
RefSeq ORF | 2592 bp |
Locus ID | 26052 |
UniProt ID | Q9UQ16 |
Cytogenetics | 1q24.3 |
Domains | dynamin_2, dynamin, PH, GED |
Protein Pathways | Endocytosis, Fc gamma R-mediated phagocytosis |
MW | 97.1 kDa |
Gene Summary | This gene encodes a member of a family of guanosine triphosphate (GTP)-binding proteins that associate with microtubules and are involved in vesicular transport. The encoded protein functions in the development of megakaryocytes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC224326L3 | Lenti ORF clone of Human dynamin 3 (DNM3), transcript variant 1, Myc-DDK-tagged |
USD 1,090.00 |
|
RC224326L4 | Lenti ORF clone of Human dynamin 3 (DNM3), transcript variant 1, mGFP tagged |
USD 1,090.00 |
|
RG224326 | DNM3 (tGFP-tagged) - Human dynamin 3 (DNM3), transcript variant 1 |
USD 990.00 |
|
SC100449 | DNM3 (untagged)-Human dynamin 3 (DNM3), transcript variant 1 |
USD 791.00 |
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