C21orf58 (NM_058180) Human Tagged ORF Clone

CAT#: RC224311

C21orf58 (Myc-DDK-tagged)-Human chromosome 21 open reading frame 58 (C21orf58)

Product Images

Circular map for RC224311

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C21orf58
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224311 representing NM_058180
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCGATCTCGGCTCCCTGCAACCTCCCTCAGGAAGCCGTGGAAGCTCGACCGCCAGAAACTTCCTT
  CTCCTGACTCAGGCCACAGTCTTCTGTGTGGCTGGTCTCCAGGAGGTAAGGCCCGCCCTGCAGGCAACAC
  CGGTGCTTGGGCTCCTGCTGAGCAGTTCTTTCCTGCGAGTAACAGAACCAGGGAGGGAGGTGGGCTGTGG
  CCTCCCCTGCCCCTACAGTCGTCTCCTGCAGCTCCCACCATGCTGGACTCATCAGCAGCAGAGCAAGTGA
  CCCGACTGACGCTGAAGCTCTTGGGACAGAAGCTGGAGCAAGAACGGCAGAACGTGGAAGGGGGACCTGA
  GGGCCTCCACCTCGAGCCAGGAAATGAGGACCGGCCGGACGATGCCCTGCAGACTGCTCTGAAGAGAAGG
  AGGGACCTTCTGCAGAGACTCCGGGAACAACACCTCCTGGACGAGCTCTCTCGGGCCCAGGCCTGGAGCG
  GGCCAAGCAGAGGAGCCCTCGGGTCAGCCCTGCCCCCAGAGCTGCCCCCCACGGGCATCCTACCCACTGC
  CTCCCCATCCCCGCTGGCCCCAGACCCGCCAAGGATCATCCTGCCTACGGTCCCCCAGCCTCCTGCCACC
  ATCATTCAGCAGCTCCCTCAGCAGCCACTCATAGCACAGATTCCTCCTCCCCAGGCCTTCCCTACTCAAC
  GGTCAGGAAGTATTAAGGAAGACATGGTGGAGCTGCTGCTGCTGCAGAACGCACAGGTGCACCAGTTGGT
  CCTGCAGAACTGGATGCTCAAGGCCCTGCCCCCAGCCCTGCAGGACCCGCCACATGTGCCCCCGAGGGTC
  CCACGAGCTGCCAGGCCAAGGCTGCCTGCCGTGCACCACCACCACCACCACCACCATGCTGTGTGGCCAC
  CTGGGGCTGCCACTGTCCTCCAGCCCGCCCCCAGCCTGTGGACGCCTGGCCCACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224311 representing NM_058180
  Red=Cloning site Green=Tags(s)
  
  MARSRLPATSLRKPWKLDRQKLPSPDSGHSLLCGWSPGGKARPAGNTGAWAPAEQFFPASNRTREGGGLW
  PPLPLQSSPAAPTMLDSSAAEQVTRLTLKLLGQKLEQERQNVEGGPEGLHLEPGNEDRPDDALQTALKRR
  RDLLQRLREQHLLDELSRAQAWSGPSRGALGSALPPELPPTGILPTASPSPLAPDPPRIILPTVPQPPAT
  IIQQLPQQPLIAQIPPPQAFPTQRSGSIKEDMVELLLLQNAQVHQLVLQNWMLKALPPALQDPPHVPPRV
  PRAARPRLPAVHHHHHHHHAVWPPGAATVLQPAPSLWTPGPP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_058180
• ORF Size: 966 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_058180.5
• RefSeq Size: 2991 bp
• RefSeq ORF: 969 bp
• Locus ID: 54058
• UniProt ID: P58505
• Cytogenetics: 21q22.3
• MW: 34.8 kDa