ATP6V0E2 (NM_001100592) Human Tagged Lenti ORF Clone
CAT#: RC224170L1
- LentiORF®
Lenti ORF clone of Human ATPase, H+ transporting V0 subunit e2 (ATP6V0E2), transcript variant 2, Myc-DDK-tagged
"NM_001100592" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | ATP6V0E2 |
Synonyms | ATP6V0E2L; C7orf32 |
Vector | pLenti-C-Myc-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC224170).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_001100592 |
ORF Size | 639 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001100592.1, NP_001094062.1 |
RefSeq Size | 2565 bp |
RefSeq ORF | 642 bp |
Locus ID | 155066 |
UniProt ID | Q8NHE4 |
Cytogenetics | 7q36.1 |
Protein Pathways | Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection |
MW | 21.8 kDa |
Gene Summary | Multisubunit vacuolar-type proton pumps, or H(+)-ATPases, acidify various intracellular compartments, such as vacuoles, clathrin-coated and synaptic vesicles, endosomes, lysosomes, and chromaffin granules. H(+)-ATPases are also found in plasma membranes of specialized cells, where they play roles in urinary acidification, bone resorption, and sperm maturation. Multiple subunits form H(+)-ATPases, with proteins of the V1 class hydrolyzing ATP for energy to transport H+, and proteins of the V0 class forming an integral membrane domain through which H+ is transported. ATP6V0E2 encodes an isoform of the H(+)-ATPase V0 e subunit, an essential proton pump component (Blake-Palmer et al., 2007 [PubMed 17350184]).[supplied by OMIM, Mar 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC224170 | ATP6V0E2 (Myc-DDK-tagged)-Human ATPase, H+ transporting V0 subunit e2 (ATP6V0E2), transcript variant 2 |
USD 300.00 |
|
RC224170L2 | Lenti ORF clone of Human ATPase, H+ transporting V0 subunit e2 (ATP6V0E2), transcript variant 2, mGFP tagged |
USD 600.00 |
|
RC224170L3 | Lenti ORF clone of Human ATPase, H+ transporting V0 subunit e2 (ATP6V0E2), transcript variant 2, Myc-DDK-tagged |
USD 600.00 |
|
RC224170L4 | Lenti ORF clone of Human ATPase, H+ transporting V0 subunit e2 (ATP6V0E2), transcript variant 2, mGFP tagged |
USD 600.00 |
|
RG224170 | ATP6V0E2 (tGFP-tagged) - Human ATPase, H+ transporting V0 subunit e2 (ATP6V0E2), transcript variant 2 |
USD 500.00 |
|
SC316681 | ATP6V0E2 (untagged)-Human ATPase, H+ transporting V0 subunit e2 (ATP6V0E2), transcript variant 2 |
USD 330.00 |
{0} Product Review(s)
Be the first one to submit a review