KCC2 (SLC12A5) (NM_020708) Human Tagged Lenti ORF Clone
CAT#: RC223680L2
- LentiORF®
Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporter), member 5 (SLC12A5), transcript variant 2, mGFP tagged
"NM_020708" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | KCC2 |
Synonyms | DEE34; EIEE34; EIG14; hKCC2; KCC2 |
Vector | pLenti-C-mGFP |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC223680).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_020708 |
ORF Size | 3348 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020708.3 |
RefSeq Size | 6059 bp |
RefSeq ORF | 3351 bp |
Locus ID | 57468 |
UniProt ID | Q9H2X9 |
Cytogenetics | 20q13.12 |
Protein Families | Transmembrane |
MW | 123.3 kDa |
Gene Summary | K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Sep 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC223680 | SLC12A5 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporter), member 5 (SLC12A5), transcript variant 2 |
USD 971.00 |
|
RC223680L1 | Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporter), member 5 (SLC12A5), transcript variant 2, Myc-DDK-tagged |
USD 1,271.00 |
|
RC223680L3 | Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporter), member 5 (SLC12A5), transcript variant 2, Myc-DDK-tagged |
USD 1,271.00 |
|
RC223680L4 | Lenti ORF clone of Human solute carrier family 12 (potassium/chloride transporter), member 5 (SLC12A5), transcript variant 2, mGFP tagged |
USD 1,271.00 |
|
RG223680 | SLC12A5 (tGFP-tagged) - Human solute carrier family 12 (potassium/chloride transporter), member 5 (SLC12A5), transcript variant 2 |
USD 1,171.00 |
|
SC304801 | SLC12A5 (untagged)-Human solute carrier family 12 (potassium/chloride transporter), member 5 (SLC12A5), transcript variant 2 |
USD 972.00 |
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