PCDHB14 (NM_018934) Human Tagged ORF Clone

CAT#: RC223094

PCDHB14 (Myc-DDK-tagged)-Human protocadherin beta 14 (PCDHB14)

Product Images

Circular map for RC223094

• 

  Western blot validation of overexpression lysate (Cat# LY412946) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223094 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PCDHB14
• Synonyms: PCDH-BETA14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223094 representing NM_018934
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATCAGAGGGGCACTCGATCTGCGAAAAAGGCAAGTTCTAATATTCCTTGTTTTGCTGGGATTGT
  CTCGGGCAGGTACTGAATCTGCACACTATTCTGTGGCAGAGGAAACAGAAATTGGCTCTTTTGTGGCTAA
  TCTAGCGAGGGACCTAGGGCTGGGGGTGGAGGAGCTGTCTTCACGTGAAGCCCGGGTAGTGTCTGATGAT
  AATAAAAAGTATTTGCACCTTGATTTGCTGACTGGGAATTTGCTCCTAAATGAGAAACTAGACCGAGACG
  AGCTGTGTGGCTCCACCGAGCCCTGTGTGCTGCATTTTCAGGTGGTTTTGGAAAACCCTTTACAGTTTTT
  TCGGTTTGAGCTGTGTGTCAAAGACATAAATGATCACTCCCCTACATTTCTAGACAAGGAAATACTTATT
  AAAATATCAGAAGGTACCACTGTTGGAGCTACCTTTCTAATGGAGAGTGCTCAAGATTTGGATGTCGGAA
  GCAACAGTCTCCAAAACTACACAATTAGCCCCAATTCTCACTTCTACATTAAAATTCCCGACAGTAGTGA
  CAGAAAGATATACCCAGAGCTGGTCCTAGATAGAGCTTTAGATTATGAACAGGAAGCTGAACTCAGATTA
  ACACTCACAGCAGTGGATGGTGGATCCCCGCCCAAGTCTGGGACAACTTTGGTTCTCATCAAGGTGTTGG
  ACATCAATGATAATGCCCCTGAGTTTCCTCAGAGTCTCTATGAGGTGCAAGTCCCCGAGGACAGACCCCT
  TGGCTCCTGGATTGCCACCATCTCAGCTAAGGATCTGGATGCAGGAAACTATGGAAAAATATCTTACACA
  TTTTTCCATGCATCAGAAGATATTCGTAAAACATTTGAAATTAATCCAATATCTGGGGAAGTTAATTTGA
  GATCACCCCTGGATTTTGAAGTAATACAGTCCTACACTATAAATATTCAGGCAACAGATGGTGGGGGTCT
  TTCAGGAAAATGCACCCTTCTAGTTAAAGTTATGGATATAAACGACAACCCACCAGAAGTGACCATATCG
  TCGATTACAAAGAGAATTCCAGAGAATGCCTCAGAGACCCTAGTAGCTCTTTTTAGTATCCTAGACCAAG
  ACTCTGGAGACAATGGGAGGATGATTTGCTCTATTCAAGATAACCTCCCTTTTTTCCTGAAACCGACCTT
  CAAGAACTTTTTCACTCTAGTTTCTGAAAAAGCACTGGACAGAGAGAGCCAAGCCGAGTACAACATCACG
  ATCACCGTCACAGACTTGGGGACACCCAGGCTGAAAACCGAGTACAACATAACCGTGCTGCTCTCTGACG
  TCAATGACAACGCCCCCACCTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCCCGC
  CCTGCACATCGGCAGCGTCAGCGCCACAGACAGAGACTCAGGCACCAACGCCCAGGTCAACTACTCGCTG
  CTGCCGCCCCAGGACCGGCACCTGCCCCTCGCCTCCTTGGTCTCCATCAACGCGGACAATGGCCACCTGT
  TTGCCCTCAGGTCGCTGGACTACGAGGCCCTACAGGAGTTCGAGTTTCGCGTGGGCGCCACAGACCGCGG
  GTCCCCGGCGTTGAGCAGCGAGGCGCTGGTGCGCGTGCTGGTGCTGGACGCCAACGACAACTCGCCCTTC
  GTGCTGTACCCGCTGCAGAACGGCTCCGCGCCCTGCACCGAGCTGGTGCCCCGGGCGGCCGAGCCGGGCT
  ACCTGGTGACCAAGGTGGTGGCGGTGGACGGCGACTCGGGCCAGAACGCCTGGCTGTCGTACCAGCTGCT
  CAAGGCCACGGAGCCCGGGCTGTTCGGCGTGTGGGCGCACAATGGCGAGGTGCGCACCGCCAGGCTGCTG
  AGCGAGCGCGACGCGGCCAAGCACAGGCTGGTGGTGCTGGTCAAGGACAATGGCGAGCCTCCGCGCTCGG
  CCACCGCCACGCTGCACGTGCTCCTGGTGGACGGCTTCTCCCAGCCCTACCTGCCGCTCCCTGAGGCGGC
  CCCGGCCCAGGCCCAGGCCGACTCCCTCACCGTCTACCTGGTGGTGGCATTGGCCTCGGTGTCGTCGCTC
  TTCCTCTTCTCGGTGCTCCTGTTCGTGGCGGTGCGGCTGTGCAGGAGGAGCAGGGCGGCCTCGGTGGGTC
  GCTGCTCGGTGCCCGAGGGTCCCTTTCCAGGGCATCTGGTGGACGTGAGCGGCACCGGGACCCTGTCCCA
  GAGCTACCAATACGAGGTGTGTCTGACAGGAGGTTCCGGGACAAATGAGTTCAAATTTCTGAAGCCGATT
  ATCCCCAATTTTCAAGTTCATGACACTGGTAGGAATATGGGGGAAATCGAGAACTTTCGAAATAGCTTTG
  GACTTAACATTCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223094 representing NM_018934
  Red=Cloning site Green=Tags(s)
  
  MEIRGALDLRKRQVLIFLVLLGLSRAGTESAHYSVAEETEIGSFVANLARDLGLGVEELSSREARVVSDD
  NKKYLHLDLLTGNLLLNEKLDRDELCGSTEPCVLHFQVVLENPLQFFRFELCVKDINDHSPTFLDKEILI
  KISEGTTVGATFLMESAQDLDVGSNSLQNYTISPNSHFYIKIPDSSDRKIYPELVLDRALDYEQEAELRL
  TLTAVDGGSPPKSGTTLVLIKVLDINDNAPEFPQSLYEVQVPEDRPLGSWIATISAKDLDAGNYGKISYT
  FFHASEDIRKTFEINPISGEVNLRSPLDFEVIQSYTINIQATDGGGLSGKCTLLVKVMDINDNPPEVTIS
  SITKRIPENASETLVALFSILDQDSGDNGRMICSIQDNLPFFLKPTFKNFFTLVSEKALDRESQAEYNIT
  ITVTDLGTPRLKTEYNITVLLSDVNDNAPTFTQTSYTLFVRENNSPALHIGSVSATDRDSGTNAQVNYSL
  LPPQDRHLPLASLVSINADNGHLFALRSLDYEALQEFEFRVGATDRGSPALSSEALVRVLVLDANDNSPF
  VLYPLQNGSAPCTELVPRAAEPGYLVTKVVAVDGDSGQNAWLSYQLLKATEPGLFGVWAHNGEVRTARLL
  SERDAAKHRLVVLVKDNGEPPRSATATLHVLLVDGFSQPYLPLPEAAPAQAQADSLTVYLVVALASVSSL
  FLFSVLLFVAVRLCRRSRAASVGRCSVPEGPFPGHLVDVSGTGTLSQSYQYEVCLTGGSGTNEFKFLKPI
  IPNFQVHDTGRNMGEIENFRNSFGLNIQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018934
• ORF Size: 2394 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018934.3
• RefSeq Size: 2793 bp
• RefSeq ORF: 2397 bp
• Locus ID: 56122
• UniProt ID: Q9Y5E9
• Cytogenetics: 5q31.3
• Domains: CA
• Protein Families: Transmembrane
• MW: 84.5 kDa
• Gene Summary: This gene is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. The extracellular domains interact in a homophilic manner to specify differential cell-cell connections. Unlike the alpha and gamma clusters, the transcripts from these genes are made up of only one large exon, not sharing common 3' exons as expected. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins. Their specific functions are unknown but they most likely play a critical role in the establishment and function of specific cell-cell neural connections. [provided by RefSeq, Jul 2008]