Connexin 40.1 (GJD4) (NM_153368) Human Tagged ORF Clone

CAT#: RC222438

GJD4 (Myc-DDK-tagged)-Human gap junction protein, delta 4, 40.1kDa (GJD4)

Product Images

Circular map for RC222438

• 

  Western blot validation of overexpression lysate (Cat# LY407080) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222438 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Connexin 40.1
• Synonyms: CX40.1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222438 representing NM_153368
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGCGTGGACTTGCTAGGGTTTCTCATCATCACATTAAACTGCAACGTGACCATGGTGGGAAAGC
  TCTGGTTCGTCCTCACGATGCTGCTGCGGATGCTGGTGATTGTCTTGGCGGGGCGACCCGTCTACCAGGA
  CGAGCAGGAGAGGTTTGTCTGCAACACGCTGCAGCCGGGATGCGCCAATGTTTGCTACGACGTCTTCTCC
  CCCGTGTCTCACCTGCGGTTCTGGCTGATCCAGGGCGTGTGCGTCCTCCTCCCCTCCGCCGTCTTCAGCG
  TCTATGTCCTGCACCGAGGAGCCACGCTCGCCGCGCTGGGCCCCCGCCGCTGCCCCGACCCCCGGGAGCC
  GGCCTCCGGGCAGAGACGCTGCCCGCGGCCATTCGGGGAGCGCGGCGGCCTCCAGGTGCCCGACTTTTCG
  GCCGGCTACATCATCCACCTCCTCCTCCGGACCCTGCTGGAGGCAGCCTTCGGGGCCTTGCACTACTTTC
  TCTTTGGATTCCTGGCCCCGAAGAAGTTCCCTTGCACGCGCCCTCCGTGCACGGGCGTGGTGGACTGCTA
  CGTGTCGCGGCCCACAGAGAAGTCCCTGCTGATGCTGTTCCTCTGGGCGGTCAGCGCGCTGTCTTTTCTG
  CTGGGCCTCGCCGACCTGGTCTGCAGCCTGCGGCGGCGGATGCGCAGGAGGCCGGGACCCCCCACAAGCC
  CCTCCATCCGGAAGCAGAGCGGAGCCTCAGGCCACGCGGAGGGACGCCGGACTGACGAGGAGGGTGGGCG
  GGAGGAAGAGGGGGCACCGGCGCCCCCGGGTGCACGCGCCGGAGGGGAGGGGGCTGGCAGCCCCAGGCGT
  ACATCCAGGGTGTCAGGGCACACGAAGATTCCGGATGAGGATGAGAGTGAGGTGACATCCTCCGCCAGCG
  AAAAGCTGGGCAGACAGCCCCGGGGCAGGCCCCACCGAGAGGCCGCCCAGGACCCCAGGGGCTCAGGATC
  CGAGGAGCAGCCCTCAGCAGCCCCCAGCCGCCTGGCCGCGCCCCCTTCCTGCAGCAGCCTGCAGCCCCCT
  GACCCGCCTGCCAGCTCCAGTGGTGCTCCCCACCTGAGAGCCAGGAAGTCTGAGTGGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222438 representing NM_153368
  Red=Cloning site Green=Tags(s)
  
  MEGVDLLGFLIITLNCNVTMVGKLWFVLTMLLRMLVIVLAGRPVYQDEQERFVCNTLQPGCANVCYDVFS
  PVSHLRFWLIQGVCVLLPSAVFSVYVLHRGATLAALGPRRCPDPREPASGQRRCPRPFGERGGLQVPDFS
  AGYIIHLLLRTLLEAAFGALHYFLFGFLAPKKFPCTRPPCTGVVDCYVSRPTEKSLLMLFLWAVSALSFL
  LGLADLVCSLRRRMRRRPGPPTSPSIRKQSGASGHAEGRRTDEEGGREEEGAPAPPGARAGGEGAGSPRR
  TSRVSGHTKIPDEDESEVTSSASEKLGRQPRGRPHREAAQDPRGSGSEEQPSAAPSRLAAPPSCSSLQPP
  DPPASSSGAPHLRARKSEWV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153368
• ORF Size: 1110 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153368.3
• RefSeq Size: 1580 bp
• RefSeq ORF: 1113 bp
• Locus ID: 219770
• UniProt ID: Q96KN9
• Cytogenetics: 10p11.21
• Protein Families: Transmembrane
• MW: 40 kDa
• Gene Summary: Connexins, such as GJD4, are involved in the formation of gap junctions, intercellular conduits that directly connect the cytoplasms of contacting cells. Each gap junction channel is formed by docking of 2 hemichannels, each of which contains 6 connexin subunits (Sohl et al., 2003 [PubMed 12881038]).[supplied by OMIM, Mar 2008]