Nebulette (NEBL) (NM_006393) Human Tagged ORF Clone

CAT#: RC222431

NEBL (Myc-DDK-tagged)-Human nebulette (NEBL), transcript variant 1

Product Images

Circular map for RC222431

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nebulette
• Synonyms: bA165O3.1; C10orf113; LASP2; LNEBL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222431 representing NM_006393
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGTCCCTGTATTTGAGGATATAAAAGATGAAACTGAAGAAGAAAAGATAGGGGAAGAAGAAAATG
  AAGAAGACCAGGTCTTCTATAAGCCTGTTATTGAAGACTTAAGCATGGAATTGGCCAGAAAATGCACGGA
  ACTCATTAGCGATATCCGTTATAAAGAAGAGTTTAAAAAGTCCAAGGATAAGTGTACATTTGTGACTGAC
  AGTCCTATGCTAAACCATGTAAAAAATATCGGTGCTTTTATTTCTGAGGCAAAATACAAAGGCACCATTA
  AAGCTGACCTTTCTAATTCTCTTTATAAGCGGATGCCAGCCACAATTGACAGTGTTTTTGCAGGAGAAGT
  TACACAGCTCCAGAGTGAGGTGGCCTACAAGCAGAAACATGATGCTGCCAAAGGATTCTCAGATTATGCC
  CACATGAAGGAGCCCCCTGAGGTTAAACATGCCATGGAGGTCAATAAACACCAGAGTAATATTTCTTATA
  GGAAAGACGTGCAGGACACCCACACGTACAGTGCAGAACTTGACCGACCAGACATCAAGATGGCAACCCA
  GATCTCTAAGATCATAAGCAATGCAGAATACAAGAAAGGACAAGGAATAATGAATAAAGAGCCCGCTGTA
  ATTGGAAGACCAGATTTTGAACATGCCGTGGAAGCTTCTAAACTTTCTAGTCAAATTAAATACAAAGAAA
  AATTCGATAATGAAATGAAGGATAAGAAACATCATTACAATCCTCTTGAAAGTGCTTCTTTTAGGCAGAA
  TCAGCTTGCTGCTACACTGGCGAGCAATGTGAAGTACAAGAAAGACATTCAAAATATGCATGATCCAGTT
  TCAGATCTCCCAAATTTGTTGTTTTTAGACCATGTTTTGAAAGCCAGCAAAATGCTCAGCGGCCGAGAAT
  ATAAAAAGCTCTTTGAGGAAAACAAAGGAATGTATCATTTTGATGCAGATGCTGTGGAACATCTGCACCA
  TAAAGGCAATGCCGTCCTCCAAAGTCAGGTGAAATATAAAGAAGAATATGAGAAAAATAAGGGAAAGCCA
  ATGCTTGAATTTGTTGAGACACCATCATATCAAGCTTCAAAGGAGGCTCAAAAGATGCAAAGTGAAAAAG
  TTTACAAAGAGGATTTTGAGAAGGAGATTAAAGGAAGGTCATCACTGGATTTAGACAAGACTCCAGAATT
  TTTACATGTAAAGTACATCACCAACCTTCTGAGGGAGAAAGAATATAAAAAAGATTTGGAAAATGAGATA
  AAAGGGAAAGGAATGGAACTTAATTCAGAAGTTCTTGATATCCAAAGAGCAAAGCGGGCCTCTGAAATGG
  CAAGTGAGAAAGAATACAAGAAAGACCTGGAGTCAATAATTAAAGGGAAAGGAATGCAAGCTGGCACTGA
  CACCCTTGAAATGCAGCATGCCAAGAAGGCTGCAGAGATAGCGAGTGAGAAAGACTATAAAAGAGATCTG
  GAGACTGAAATTAAAGGGAAAGGGATGCAGGTGAGCACAGACACTCTTGATGTCCAGAGAGCTAAGAAAG
  CATCCGAGATGGCCAGCCAGAAACAATACAAGAAGGACTTAGAAAATGAAATTAAAGGGAAAGGAATGCA
  AGTGAGCATGGATATCCCAGATATCCTTCGAGCCAAGAGGACATCTGAAATCTATAGCCAGAGAAAGTAT
  AAAGATGAAGCAGAGAAGATGCTTTCTAACTATTCTACCATAGCAGATACTCCTGAAATTCAGAGAATTA
  AGACAACTCAACAAAACATTAGTGCGGTATTTTATAAGAAAGAAGTGGGAGCTGGCACTGCAGTGAAAGA
  TAGCCCAGAGATCGAACGAGTGAAGAAAAATCAGCAGAATATTAGTTCAGTGAAATACAAAGAAGAGATT
  AAACATGCAACAGCCATTTCTGATCCTCCAGAACTAAAGAGAGTTAAAGAAAACCAGAAGAACATCAGCA
  ATCTCCAGTATAAAGAGCAAAACTACAAGGCCACTCCGGTAAGCATGACCCCGGAGATAGAGAGAGTGAG
  GCGAAACCAGGAGCAGCTGAGTGCGGTAAAATATAAGGGAGAACTTCAACGGGGAACTGCAATTTCTGAT
  CCACCAGAGCTGAAGAGGGCAAAAGAAAACCAGAAAAACATCAGCAATGTTTATTACAGAGGTCAGCTGG
  GAAGAGCTACCACTTTAAGTGTAACTCCTGAAATGGAAAGAGTGAAGAAGAATCAAGAAAATATTAGCTC
  GGTAAAATATACCCAGGACCATAAACAGATGAAAGGTAGACCAAGTCTGATTTTAGATACACCTGCTATG
  AGACATGTTAAAGAAGCACAAAATCATATTTCAATGGTAAAATACCATGAAGATTTTGAAAAAACAAAGG
  GGAGAGGCTTTACTCCCGTCGTGGACGATCCTGTGACAGAGAGAGTGAGGAAGAACACCCAGGTGGTCAG
  CGATGCTGCCTATAAAGGGGTCCACCCTCACATCGTGGAGATGGACAGGAGACCTGGAATCATTGTTGAC
  CTCAAAGTTTGGCGCACAGATCCTGGCTCCATCTTCGACCTTGATCCCCTGGAAGACAATATTCAGTCTA
  GAAGTCTCCATATGCTCTCTGAAAAGGCGAGTCACTATAGGCGACACTGGTCTCGATCCCATTCCAGCAG
  TACTTTCGGTACAGGTCTCGGAGACGACAGGTCAGAAATCTCCGAGATTTACCCTAGCTTTTCATGCTGC
  AGTGAGGTAACAAGACCGTCTGATGAAGGAGCACCTGTTCTTCCCGGAGCCTATCAGCAAAGCCATTCCC
  AAGGCTATGGCTACATGCACCAGACCAGTGTGTCATCCATGAGATCAATGCAGCATTCACCAAATCTAAG
  GACCTACCGAGCCATGTACGATTACAGTGCCCAGGATGAAGACGAGGTCTCCTTTAGAGACGGCGACTAC
  ATCGTCAACGTGCAGCCTATTGACGATGGCTGGATGTACGGCACAGTGCAGAGAACAGGGAGAACAGGAA
  TGCTCCCAGCGAATTACATTGAGTTTGTTAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222431 representing NM_006393
  Red=Cloning site Green=Tags(s)
  
  MRVPVFEDIKDETEEEKIGEEENEEDQVFYKPVIEDLSMELARKCTELISDIRYKEEFKKSKDKCTFVTD
  SPMLNHVKNIGAFISEAKYKGTIKADLSNSLYKRMPATIDSVFAGEVTQLQSEVAYKQKHDAAKGFSDYA
  HMKEPPEVKHAMEVNKHQSNISYRKDVQDTHTYSAELDRPDIKMATQISKIISNAEYKKGQGIMNKEPAV
  IGRPDFEHAVEASKLSSQIKYKEKFDNEMKDKKHHYNPLESASFRQNQLAATLASNVKYKKDIQNMHDPV
  SDLPNLLFLDHVLKASKMLSGREYKKLFEENKGMYHFDADAVEHLHHKGNAVLQSQVKYKEEYEKNKGKP
  MLEFVETPSYQASKEAQKMQSEKVYKEDFEKEIKGRSSLDLDKTPEFLHVKYITNLLREKEYKKDLENEI
  KGKGMELNSEVLDIQRAKRASEMASEKEYKKDLESIIKGKGMQAGTDTLEMQHAKKAAEIASEKDYKRDL
  ETEIKGKGMQVSTDTLDVQRAKKASEMASQKQYKKDLENEIKGKGMQVSMDIPDILRAKRTSEIYSQRKY
  KDEAEKMLSNYSTIADTPEIQRIKTTQQNISAVFYKKEVGAGTAVKDSPEIERVKKNQQNISSVKYKEEI
  KHATAISDPPELKRVKENQKNISNLQYKEQNYKATPVSMTPEIERVRRNQEQLSAVKYKGELQRGTAISD
  PPELKRAKENQKNISNVYYRGQLGRATTLSVTPEMERVKKNQENISSVKYTQDHKQMKGRPSLILDTPAM
  RHVKEAQNHISMVKYHEDFEKTKGRGFTPVVDDPVTERVRKNTQVVSDAAYKGVHPHIVEMDRRPGIIVD
  LKVWRTDPGSIFDLDPLEDNIQSRSLHMLSEKASHYRRHWSRSHSSSTFGTGLGDDRSEISEIYPSFSCC
  SEVTRPSDEGAPVLPGAYQQSHSQGYGYMHQTSVSSMRSMQHSPNLRTYRAMYDYSAQDEDEVSFRDGDY
  IVNVQPIDDGWMYGTVQRTGRTGMLPANYIEFVN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006393
• ORF Size: 3042 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006393.1, NP_006384.1
• RefSeq Size: 8034 bp
• RefSeq ORF: 3045 bp
• Locus ID: 10529
• UniProt ID: O76041
• Cytogenetics: 10p12.31
• MW: 116.3 kDa
• Gene Summary: This gene encodes a nebulin like protein that is abundantly expressed in cardiac muscle. The encoded protein binds actin and interacts with thin filaments and Z-line associated proteins in striated muscle. This protein may be involved in cardiac myofibril assembly. A shorter isoform of this protein termed LIM nebulette is expressed in non-muscle cells and may function as a component of focal adhesion complexes. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]