APXL (SHROOM2) (NM_001649) Human Tagged ORF Clone

CAT#: RC222295

SHROOM2 (Myc-DDK-tagged)-Human shroom family member 2 (SHROOM2)

Product Images

Circular map for RC222295

• 

  Western blot validation of overexpression lysate (Cat# LY419824) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222295 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: APXL
• Synonyms: APXL; HSAPXL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222295 representing NM_001649
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGCGCCGAGCCCCGCGCGCGGCCCGAGCGCCTGGCCGAGGCCGAGACGCGGGCGGCGGACGGCG
  GGCGCCTGGTGGAGGTGCAGCTGAGCGGCGGCGCCCCGTGGGGCTTCACCCTGAAGGGCGGCCGCGAGCA
  CGGCGAGCCGCTGGTCATCACCAAGATTGAAGAGGGCAGTAAAGCCGCGGCGGTCGACAAGTTACTGGCT
  GGAGATGAGATCGTCGGCATCAATGACATTGGTCTCTCAGGGTTTAGACAGGAAGCGATTTGCCTGGTGA
  AGGGGTCCCATAAGACCCTGAAGCTGGTCGTCAAAAGGAGGAGCGAGCTGGGCTGGAGGCCTCACTCCTG
  GCATGCCACCAAGTTCTCTGACAGCCACCCCGAGCTAGCGGCCTCCCCATTCACCTCCACCAGCGGCTGT
  CCTTCCTGGTCCGGCCGACACCACGCGAGTTCTTCCTCCCACGACCTGTCCAGTTCCTGGGAGCAGACGA
  ACCTACAGCGCACCTTAGATCACTTCAGCTCCTTGGGGAGCGTTGACAGCCTGGACCACCCCTCCAGTCG
  CCTCTCGGTGGCCAAGTCCAACAGCAGCATCGACCACCTGGGCAGCCACAGCAAGCGCGACTCGGCCTAC
  GGCTCCTTCTCCACCAGCTCTAGCACTCCTGACCACACCTTGTCCAAAGCCGACACGTCCTCCGCAGAGA
  ACATCCTCTACACTGTGGGCCTCTGGGAGGCTCCCAGGCAGGGTGGCCGGCAGGCCCAGGCCGCAGGCGA
  CCCTCAGGGCTCGGAGGAGAAGCTCAGTTGTTTCCCGCCCAGGGTCCCCGGTGACAGCGGCAAAGGCCCC
  AGGCCAGAGTACAATGCCGAGCCCAAGCTGGCTGCCCCTGGGAGGTCCAATTTTGGGCCAGTCTGGTATG
  TTCCCGATAAGAAGAAAGCACCATCATCCCCACCTCCTCCCCCTCCCCCTCTCCGCAGTGACAGCTTTGC
  TGCCACCAAGAGCCACGAGAAGGCCCAGGGCCCTGTGTTCTCAGAGGCGGCTGCGGCACAGCACTTTACG
  GCCCTGGCCCAGGCTCAGCCTCGTGGTGACCGGAGACCAGAGCTCACCGATCGGCCTTGGAGGTCAGCAC
  ACCCGGGGAGCCTCGGGAAGGGATCGGGAGGCCCGGGCTGCCCACAGGAGGCCCACGCAGACGGCAGCTG
  GCCGCCCTCCAAGGATGGAGCTTCCAGTAGGCTGCAGGCCTCTCTGTCCAGCTCAGATGTGCGCTTCCCT
  CAGTCTCCTCATAGCGGCCGACACCCTCCCCTATACAGCGACCACAGCCCCCTCTGTGCTGACAGCCTTG
  GGCAGGAGCCAGGGGCTGCCAGCTTCCAGAACGACAGCCCTCCTCAGGTGAGGGGGCTCAGCAGCTGTGA
  CCAGAAGCTGGGGAGCGGCTGGCAGGGTCCCCGGCCCTGTGTGCAGGGAGACCTGCAAGCAGCACAGCTC
  TGGGCGGGATGCTGGCCTTCTGACACAGCCCTTGGAGCCCTCGAGAGTCTTCCCCCACCCACGGTGGGCC
  AGAGCCCACGCCATCACCTACCTCAGCCTGAGGGTCCTCCGGATGCCCGCGAGACAGGACGGTGTTACCC
  GCTGGACAAAGGGGCCGAGGGCTGCTCCGCGGGAGCCCAGGAGCCTCCCAGGGCCAGCCGTGCAGAAAAA
  GCCAGCCAGAGGCTGGCAGCCAGCATCACGTGGGCAGATGGGGAGAGCAGCAGGATCTGCCCGCAGGAGA
  CGCCCCTGTTGCACTCCCTGACCCAGGAGGGGAAGCGCCGGCCTGAGAGCAGTCCAGAGGACAGCGCCAC
  CAGACCGCCACCGTTCGACGCCCACGTGGGCAAGCCCACCCGAAGAAGCGACCGCTTTGCCACCACCCTG
  CGGAATGAGATCCAGATGCATAGAGCCAAGCTGCAGAAGAGCCGGAGCACAGTGGCTCTGACTGCAGCAG
  GGGAGGCGGAGGATGGCACCGGCCGCTGGAGGGCCGGGTTGGGAGGTGGCACCCAGGAAGGACCCCTCGC
  TGGCACCTATAAAGACCACCTGAAAGAGGCCCAAGCCCGGGTCCTGAGGGCCACGTCCTTCAAGCGCCGC
  GACTTGGACCCCAACCCAGGAGACCTATACCCGGAGTCACTGGAACACCGGATGGGGGATCCAGACACTG
  TCCCCCACTTCTGGGAGGCAGGCCTGGCCCAGCCACCCTCATCTACAAGTGGCGGGCCCCACCCGCCCCG
  CATCGGAGGCCGGAGACGGTTCACAGCTGAGCAGAAATTGAAGTCCTACTCGGAACCTGAGAAGATGAAC
  GAGGTGGGCCTCACGAGGGGCTACAGTCCTCACCAGCACCCCAGGACATCTGAGGATACTGTGGGCACGT
  TTGCTGACAGGTGGAAGTTTTTTGAGGAAACGAGCAAACCTGTTCCCCAGAGGCCTGCCCAGAAGCAAGC
  TCTTCACGGAATCCCGAGAGACAAGCCAGAGAGGCCGCGGACAGCGGGCCGCACATGTGAGGGCACGGAG
  CCCTGGTCGCGCACCACCTCCCTTGGGGACAGCCTCAACGCTCACAGCGCAGCGGAGAAGGCAGGGACTT
  CAGACCTGCCGCGGAGGCTCGGCACCTTTGCAGAGTATCAGGCCTCTTGGAAGGAACAGAGGAAACCTCT
  GGAGGCCAGGAGCTCTGGGCGCTGCCACTCAGCGGATGACATCCTGGATGTGAGCCTGGACCCACAGGAG
  AGGCCGCAGCACGTTCATGGGAGGTCCCGGTCTTCACCGTCCACAGACCACTACAAGCAGGAAGCTTCTG
  TCGAACTGCGAAGGCAGGCAGGGGACCCCGGCGAGCCCAGAGAAGAGCTTCCCTCCGCAGTCCGGGCCGA
  GGAGGGACAGTCCACGCCGAGACAAGCAGATGCCCAGTGTCGGGAAGGCAGCCCAGGATCACAGCAGCAC
  CCACCGAGTCAGAAGGCACCGAACCCACCCACATTCTCTGAACTATCTCACTGCCGGGGAGCCCCAGAGC
  TGCCCCGGGAGGGCCGGGGCCGAGCGGGAACCCTACCTCGAGATTATAGATACTCGGAGGAGAGCACCCC
  AGCAGACTTGGGACCCCGAGCCCAGAGCCCTGGCTCACCCCTGCATGCTCGAGGACAAGACTCGTGGCCA
  GTGAGCTCAGCCCTGCTCTCCAAGAGGCCAGCCCCACAGAGGCCACCGCCACCCAAGCGCGAGCCCAGGA
  GATACAGGGCCACAGACGGCGCACCTGCTGACGCCCCCGTGGGCGTCCTCGGCAGGCCCTTCCCAACGCC
  ATCCCCTGCGTCCCTGGATGTGTATGTGGCCCGCCTGTCCCTCTCCCACAGCCCCTCTGTGTTCAGCAGT
  GCCCAGCCCCAGGACACCCCGAAGGCCACTGTCTGTGAGCGTGGAAGCCAGCATGTGAGCGGGGACGCAT
  CACGTCCTCTGCCAGAAGCACTGCTCCCTCCCAAGCAGCAGCACCTGCGCCTGCAGACGGCCACCATGGA
  GACCTCGCGCTCCCCCTCGCCCCAGTTCGCCCCCCAGAAACTGACGGACAAACCTCCCCTGCTCATCCAG
  GATGAGGATTCAACCAGAATTGAGCGGGTGATGGACAACAACACCACGGTGAAGATGGTGCCCATCAAGA
  TCGTGCACTCGGAGAGCCAGCCAGAGAAGGAGAGCCGCCAGAGCCTGGCATGCCCCGCCGAGCCACCTGC
  CCTGCCCCACGGGCTGGAGAAAGACCAGATCAAGACGCTGAGCACATCTGAGCAGTTCTACTCGCGCTTC
  TGTCTGTACACGCGGCAGGGTGCTGAGCCCGAGGCCCCACATAGGGCCCAGCCGGCTGAGCCCCAGCCCC
  TGGGCACCCAGGTGCCCCCCGAGAAAGACCGCTGCACCTCCCCTCCAGGGCTCAGCTACATGAAGGCCAA
  AGAGAAGACTGTGGAAGACCTGAAGTCGGAGGAGCTGGCCAGGGAGATCGTGGGGAAGGATAAGTCCCTG
  GCCGACATCCTGGATCCCAGTGTGAAGATCAAAACCACTATGGACTTGATGGAAGGCATCTTCCCCAAAG
  ACGAGCACCTCCTGGAAGAAGCCCAGCAACGGAGGAAGCTGCTCCCCAAAATCCCCTCTCCTAGAAGCAC
  AGAGGAGAGGAAAGAGGAGCCCAGCGTGCCTGCGGCCGTGTCCCTGGCCACCAATTCTACCTACTACAGC
  ACGTCGGCCCCCAAGGCGGAGCTGCTGATCAAGATGAAGGACCTGCAGGAGCAGCAGGAGCACGAAGAGG
  ATTCGGGAAGCGACTTGGACCACGACCTGTCGGTGAAGAAGCAGGAGCTCATCGAGAGCATCAGCCGCAA
  GCTGCAGGTGCTCCGGGAGGCCCGCGAGAGCCTGCTGGAGGACGTGCAGGCCAACACCGTGCTGGGGGCC
  GAGGTGGAGGCCATCGTGAAAGGCGTCTGCAAGCCCAGCGAGTTTGACAAGTTCCGGATGTTCATTGGAG
  ACCTGGACAAAGTGGTGAACCTCCTGCTGTCGCTGTCAGGCCGCCTGGCCCGGGTGGAGAATGCCCTCAA
  TAATTTGGACGACGGCGCTTCTCCCGGTGATCGGCAATCACTGCTTGAGAAGCAGAGAGTCCTGATCCAG
  CAGCACGAGGACGCCAAGGAGCTCAAGGAGAACCTGGACCGCCGCGAGCGCATCGTCTTTGACATTTTGG
  CCAACTATCTGAGCGAGGAGAGCCTCGCGGACTATGAGCACTTCGTGAAGATGAAGTCGGCCCTCATCAT
  CGAGCAGCGGGAGCTGGAAGATAAAATCCACCTTGGTGAAGAGCAGCTGAAGTGCTTATTGGACAGCCTT
  CAGCCCGAAAGGGGCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222295 representing NM_001649
  Red=Cloning site Green=Tags(s)
  
  MEGAEPRARPERLAEAETRAADGGRLVEVQLSGGAPWGFTLKGGREHGEPLVITKIEEGSKAAAVDKLLA
  GDEIVGINDIGLSGFRQEAICLVKGSHKTLKLVVKRRSELGWRPHSWHATKFSDSHPELAASPFTSTSGC
  PSWSGRHHASSSSHDLSSSWEQTNLQRTLDHFSSLGSVDSLDHPSSRLSVAKSNSSIDHLGSHSKRDSAY
  GSFSTSSSTPDHTLSKADTSSAENILYTVGLWEAPRQGGRQAQAAGDPQGSEEKLSCFPPRVPGDSGKGP
  RPEYNAEPKLAAPGRSNFGPVWYVPDKKKAPSSPPPPPPPLRSDSFAATKSHEKAQGPVFSEAAAAQHFT
  ALAQAQPRGDRRPELTDRPWRSAHPGSLGKGSGGPGCPQEAHADGSWPPSKDGASSRLQASLSSSDVRFP
  QSPHSGRHPPLYSDHSPLCADSLGQEPGAASFQNDSPPQVRGLSSCDQKLGSGWQGPRPCVQGDLQAAQL
  WAGCWPSDTALGALESLPPPTVGQSPRHHLPQPEGPPDARETGRCYPLDKGAEGCSAGAQEPPRASRAEK
  ASQRLAASITWADGESSRICPQETPLLHSLTQEGKRRPESSPEDSATRPPPFDAHVGKPTRRSDRFATTL
  RNEIQMHRAKLQKSRSTVALTAAGEAEDGTGRWRAGLGGGTQEGPLAGTYKDHLKEAQARVLRATSFKRR
  DLDPNPGDLYPESLEHRMGDPDTVPHFWEAGLAQPPSSTSGGPHPPRIGGRRRFTAEQKLKSYSEPEKMN
  EVGLTRGYSPHQHPRTSEDTVGTFADRWKFFEETSKPVPQRPAQKQALHGIPRDKPERPRTAGRTCEGTE
  PWSRTTSLGDSLNAHSAAEKAGTSDLPRRLGTFAEYQASWKEQRKPLEARSSGRCHSADDILDVSLDPQE
  RPQHVHGRSRSSPSTDHYKQEASVELRRQAGDPGEPREELPSAVRAEEGQSTPRQADAQCREGSPGSQQH
  PPSQKAPNPPTFSELSHCRGAPELPREGRGRAGTLPRDYRYSEESTPADLGPRAQSPGSPLHARGQDSWP
  VSSALLSKRPAPQRPPPPKREPRRYRATDGAPADAPVGVLGRPFPTPSPASLDVYVARLSLSHSPSVFSS
  AQPQDTPKATVCERGSQHVSGDASRPLPEALLPPKQQHLRLQTATMETSRSPSPQFAPQKLTDKPPLLIQ
  DEDSTRIERVMDNNTTVKMVPIKIVHSESQPEKESRQSLACPAEPPALPHGLEKDQIKTLSTSEQFYSRF
  CLYTRQGAEPEAPHRAQPAEPQPLGTQVPPEKDRCTSPPGLSYMKAKEKTVEDLKSEELAREIVGKDKSL
  ADILDPSVKIKTTMDLMEGIFPKDEHLLEEAQQRRKLLPKIPSPRSTEERKEEPSVPAAVSLATNSTYYS
  TSAPKAELLIKMKDLQEQQEHEEDSGSDLDHDLSVKKQELIESISRKLQVLREARESLLEDVQANTVLGA
  EVEAIVKGVCKPSEFDKFRMFIGDLDKVVNLLLSLSGRLARVENALNNLDDGASPGDRQSLLEKQRVLIQ
  QHEDAKELKENLDRRERIVFDILANYLSEESLADYEHFVKMKSALIIEQRELEDKIHLGEEQLKCLLDSL
  QPERGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001649
• ORF Size: 4848 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001649.4
• RefSeq Size: 7445 bp
• RefSeq ORF: 4851 bp
• Locus ID: 357
• UniProt ID: Q13796
• Cytogenetics: Xp22.2
• Protein Families: Druggable Genome
• MW: 176.2 kDa
• Gene Summary: This gene represents the human homolog of Xenopus laevis apical protein (APX) gene, which is implicated in amiloride-sensitive sodium channel activity. It is expressed in endothelial cells and facilitates the formation of a contractile network within endothelial cells. Depletion of this gene results in an increase in endothelial sprouting, migration, and angiogenesis. This gene is highly expressed in the retina, and is a strong candidate for ocular albinism type 1 syndrome. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2016]