FBXO41 (NM_001080410) Human Tagged ORF Clone

CAT#: RC222120

FBXO41 (Myc-DDK-tagged)-Human F-box protein 41 (FBXO41)

Product Images

Circular map for RC222120

• 

  Western blot validation of overexpression lysate (Cat# LY421627) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222120 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FBXO41
• Synonyms: FBX41
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222120 representing NM_001080410
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCGCTGGACCTGCCGTACCGCTGCCCCCGCTGCGGGGAGCACAAGCGCTTCCGGAGCCTGTCGT
  CGCTGCGCGCGCACCTGGAGTACAGCCACACCTACGAGACGCTCTACATCCTCTCCAAGACCAACAGCAT
  CTGCGACGGCGCCGCCGCCGCCGCGGCCGCCGCCGCCGCTGCCTCGGGGTTCCCGCTGGCTCCCGAGCCC
  GCCGCCCTGCTGGCCGTGCCCGGCGCCCGGCGAGAGGTCTTCGAGAGCACTTCCTTCCAGGGCAAGGAGC
  AGGCGGCCGGGCCGTCGCCCGCGGCGCCGCACCTGCTGCACCACCACCATCACCACGCTCCCCTCGCCCA
  CTTCCCCGGCGACCTGGTGCCCGCTAGCCTGCCCTGTGAGGAGTTGGCCGAGCCGGGCCTTGTGCCCGCC
  GCAGCAGCGCGCTATGCGCTGCGCGAGATCGAGATCCCGCTGGGGGAGCTGTTCGCCCGCAAGTCCGTGG
  CGTCCTCGGCGTGCTCGACGCCGCCGCCTGGCCCCGGCCCCGGCCCTTGCCCCGGGCCTGCCTCCGCTTC
  GCCCGCGTCCCCCTCACCCGCTGATGTGGCCTACGAAGAGGGCCTGGCGCGCCTCAAGATCCGCGCGCTG
  GAGAAGCTGGAGGTGGACCGGCGGCTGGAGCGGCTGAGCGAGGAGGTGGAGCAGAAGATCGCGGGCCAGG
  TGGGCCGGCTGCAGGCCGAGCTGGAGCGCAAGGCGGCCGAACTGGAGACTGCGCGGCAGGAGAGTGCGAG
  GCTCGGGCGCGAGAAGGAGGAGCTGGAGGAGCGCGCGTCTGAGCTCTCCCGCCAGGTGGACGTGAGCGTA
  GAGCTGCTGGCCTCACTCAAGCAGGACCTGGTGCACAAGGAACAGGAGCTGAGCCGCAAGCAGCAGGAGG
  TGGTGCAGATCGACCAGTTCCTGAAGGAGACGGCGGCGCGGGAGGCCAGCGCCAAGCTGCGGCTGCAGCA
  GTTCATTGAGGAGCTCCTTGAGCGGGCTGACCGTGCCGAGCGGCAGCTGCAGGTCATCAGCAGCAGCTGT
  GGCAGCACGCCCAGCGCCAGCCTGGGCCGTGGAGGTGGGGGCGGTGGTGCTGGACCCAATGCCCGGGGCC
  CAGGCAGAATGCGAGAACACCACGTGGGCCCGGCCGTGCCTAACACATATGCAGTGTCACGGCATGGCTC
  CTCTCCGAGCACAGGGGCCTCCAGCCGTGTGCCAGCCGCATCCCAGAGCTCAGGCTGCTATGACAGTGAC
  AGTCTGGAGCTGCCCAGGCCAGAGGAGGGGGCCCCTGAGGACAGTGGCCCTGGGGGCTTGGGCACACGGG
  CCCAGGCTGCCAACGGGGGCTCAGAGCGGTCCCAGCCCCCTCGCAGCTCAGGCCTGCGGCGCCAGGCCAT
  CCAGAACTGGCAGCGCAGACCCCGCCGACACAGCACTGAGGGGGAAGAGGGTGATGTCTCCGACGTTGGC
  TCCCGAACCACTGAGTCAGAGGCTGAGGGCCCGTTGGATGCGCCCCGCCCCGGGCCTGCTATGGCTGGGC
  CATTGAGCAGCTGCCGGCTCTCAGCCCGCCCCGAGGGAGGCAGTGGGCGGGGTCGGCGAGCAGAGAGGGT
  CAGCCCCTCACGCTCCAATGAGGTCATCAGCCCAGAGATCCTGAAGATGCGAGCTGCCCTCTTCTGCATC
  TTCACCTACCTGGACACGCGCACACTGCTGCATGCTGCCGAGGTCTGCCGGGACTGGCGCTTCGTGGCCC
  GCCACCCCGCAGTCTGGACAAGGGTGCTGCTTGAGAATGCCCGTGTCTGCTCCAAGTTCCTGGCAATGCT
  GGCTCAGTGGTGCACCCAGGCCCACTCTCTGACGCTGCAGAACTTGAAGCCCCGGCAGCGGGGAAAGAAG
  GAGAGCAAGGAGGAGTATGCCCGGAGCACCCGGGGCTGCCTGGAAGCTGGGCTGGAGTCCCTGCTGAAGG
  CAGCTGGGGGGAACCTGCTGATCCTGCGCATCTCCCACTGTCCAAACATCCTCACCGACCGCTCGCTCTG
  GCTGGCCAGCTGCTACTGCCGTGCCCTGCAGGCTGTCACGTACAGGAGTGCCACAGACCCCGTGGGCCAT
  GAGGTCATTTGGGCCCTGGGCGCAGGCTGCAGAGAGATCGTCTCCCTCCAAGTGGCACCACTTCACCCCT
  GCCAGCAGCCCACACGCTTCAGTAACCGCTGCCTGCAGATGATTGGTCGCTGTTGGCCCCACCTGCGGGC
  CCTGGGGGTCGGGGGTGCCGGCTGTGGGGTGCAGGGCCTGGCATCACTCGCGAGAAACTGCATGCGGCTG
  CAGGTCCTGGAGCTTGACCACGTGTCAGAGATCACCCAGGAGGTGGCAGCAGAGGTCTGCCGGGAAGGCC
  TGAAGGGACTGGAGATGCTGGTGCTCACGGCGACTCCCGTCACCCCTAAGGCCCTACTGCACTTCAACAG
  CATCTGCCGGAACCTCAAGTCCATTGTGGTCCAGATTGGGATTGCGGATTATTTCAAAGAGCCCAGCAGC
  CCTGAGGCCCAGAAGCTGTTTGAGGACATGGTGACAAAACTCCAGGCTCTGCGACGGAGGCCCGGCTTCT
  CTAAGATTCTGCACATCAAGGTGGAAGGCGGCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222120 representing NM_001080410
  Red=Cloning site Green=Tags(s)
  
  MASLDLPYRCPRCGEHKRFRSLSSLRAHLEYSHTYETLYILSKTNSICDGAAAAAAAAAAASGFPLAPEP
  AALLAVPGARREVFESTSFQGKEQAAGPSPAAPHLLHHHHHHAPLAHFPGDLVPASLPCEELAEPGLVPA
  AAARYALREIEIPLGELFARKSVASSACSTPPPGPGPGPCPGPASASPASPSPADVAYEEGLARLKIRAL
  EKLEVDRRLERLSEEVEQKIAGQVGRLQAELERKAAELETARQESARLGREKEELEERASELSRQVDVSV
  ELLASLKQDLVHKEQELSRKQQEVVQIDQFLKETAAREASAKLRLQQFIEELLERADRAERQLQVISSSC
  GSTPSASLGRGGGGGGAGPNARGPGRMREHHVGPAVPNTYAVSRHGSSPSTGASSRVPAASQSSGCYDSD
  SLELPRPEEGAPEDSGPGGLGTRAQAANGGSERSQPPRSSGLRRQAIQNWQRRPRRHSTEGEEGDVSDVG
  SRTTESEAEGPLDAPRPGPAMAGPLSSCRLSARPEGGSGRGRRAERVSPSRSNEVISPEILKMRAALFCI
  FTYLDTRTLLHAAEVCRDWRFVARHPAVWTRVLLENARVCSKFLAMLAQWCTQAHSLTLQNLKPRQRGKK
  ESKEEYARSTRGCLEAGLESLLKAAGGNLLILRISHCPNILTDRSLWLASCYCRALQAVTYRSATDPVGH
  EVIWALGAGCREIVSLQVAPLHPCQQPTRFSNRCLQMIGRCWPHLRALGVGGAGCGVQGLASLARNCMRL
  QVLELDHVSEITQEVAAEVCREGLKGLEMLVLTATPVTPKALLHFNSICRNLKSIVVQIGIADYFKEPSS
  PEAQKLFEDMVTKLQALRRRPGFSKILHIKVEGGC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080410
• ORF Size: 2625 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080410.4
• RefSeq Size: 7121 bp
• RefSeq ORF: 2628 bp
• Locus ID: 150726
• UniProt ID: Q8TF61
• Cytogenetics: 2p13.2
• MW: 94.3 kDa
• Gene Summary: This gene encodes a member of the F-box protein family, which is characterized by an approximately 40 amino acid motif, the F-box. F-box proteins constitute one of the four subunits of the SCF ubiquitin protein ligase complex that plays a role in phosphorylation-dependent ubiquitination. F-box proteins are divided into three classes depending on the interaction substrate domain each contains in addition to the F-box motif: FBXW proteins contain WD-40 domains, FBXL proteins contain leucine-rich repeats, and FBXO proteins contain either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the FBXO class. [provided by RefSeq, Feb 2014]