TAS2R60 (NM_177437) Human Tagged ORF Clone

CAT#: RC222067

TAS2R60 (Myc-DDK-tagged)-Human taste receptor, type 2, member 60 (TAS2R60)

Product Images

Circular map for RC222067

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  Western blot validation of overexpression lysate (Cat# LY406164) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222067 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TAS2R60
• Synonyms: T2R56; T2R60
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222067 representing NM_177437
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGGAGACCACATGGTTCTAGGATCTTCGGTGACTGACAAGAAGGCCATCATCTTGGTTACCATTT
  TACTCCTTTTACGCCTGGTAGCAATAGCAGGCAATGGCTTCATCACTGCTGCTCTGGGCGTGGAGTGGGT
  GCTACGGAGAATGTTGTTGCCTTGTGATAAGTTATTGGTTAGCCTAGGGGCCTCTCGCTTCTGTCTGCAG
  TCAGTGGTAATGGGTAAGACCATTTATGTTTTCTTGCATCCGATGGCCTTCCCATACAACCCTGTACTGC
  AGTTTCTAGCTTTCCAGTGGGACTTCCTGAATGCTGCCACCTTATGGTCCTCTACCTGGCTCAGTGTCTT
  CTATTGTGTGAAAATTGCTACCTTCACCCACCCTGTCTTCTTCTGGCTAAAGCACAAGTTGTCTGGGTGG
  CTACCATGGATGCTCTTCAGCTCTGTAGGGCTCTCCAGCTTCACCACCATTCTATTTTTCATAGGCAACC
  ACAGAATGTATCAGAACTATTTAAGGAACCATCTACAACCTTGGAATGTCACTGGCGATAGCATACGGAG
  CTACTGTGAGAAATTCTATCTCTTCCCTCTAAAAATGATTACTTGGACAATGCCCACTGCTGTCTTTTTC
  ATTTGCATGATTTTGCTCATCACATCTCTGGGAAGACACAGGAAGAAGGCTCTCCTTACAACCTCAGGAT
  TCCGAGAGCCCAGTGTGCAGGCACACATAAAGGCTCTGCTGGCTCTCCTCTCTTTTGCCATGCTCTTCAT
  CTCATATTTCCTGTCACTGGTGTTCAGTGCTGCAGGTATTTTTCCACCTCTGGACTTTAAATTCTGGGTG
  TGGGAGTCAGTGATTTATCTGTGTGCAGCAGTTCACCCCATCATTCTGCTCTTCAGCAACTGCAGGCTGA
  GAGCTGTGCTGAAGAGTCGTCGTTCCTCAAGGTGTGGGACACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222067 representing NM_177437
  Red=Cloning site Green=Tags(s)
  
  MNGDHMVLGSSVTDKKAIILVTILLLLRLVAIAGNGFITAALGVEWVLRRMLLPCDKLLVSLGASRFCLQ
  SVVMGKTIYVFLHPMAFPYNPVLQFLAFQWDFLNAATLWSSTWLSVFYCVKIATFTHPVFFWLKHKLSGW
  LPWMLFSSVGLSSFTTILFFIGNHRMYQNYLRNHLQPWNVTGDSIRSYCEKFYLFPLKMITWTMPTAVFF
  ICMILLITSLGRHRKKALLTTSGFREPSVQAHIKALLALLSFAMLFISYFLSLVFSAAGIFPPLDFKFWV
  WESVIYLCAAVHPIILLFSNCRLRAVLKSRRSSRCGTP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177437
• ORF Size: 954 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177437.1, NP_803186.1
• RefSeq Size: 957 bp
• RefSeq ORF: 957 bp
• Locus ID: 338398
• UniProt ID: P59551
• Cytogenetics: 7q35
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Taste transduction
• MW: 36.2 kDa
• Gene Summary: This gene encodes a member of the bitter taste receptor family which belong to the G protein-coupled receptor superfamily and are predominantly expressed in taste receptor cells of the tongue and palate epithelia. This intronless taste receptor gene encodes a seven-transmembrane receptor protein, functioning as a bitter taste receptor. This gene is clustered together with eight other taste receptor genes on chromosome 7. [provided by RefSeq, Jul 2017]