TAS2R60 (NM_177437) Human Tagged ORF Clone
CAT#: RC222067
TAS2R60 (Myc-DDK-tagged)-Human taste receptor, type 2, member 60 (TAS2R60)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_177437" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | TAS2R60 |
Synonyms | T2R56; T2R60 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC222067 representing NM_177437
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGAGACCACATGGTTCTAGGATCTTCGGTGACTGACAAGAAGGCCATCATCTTGGTTACCATTT TACTCCTTTTACGCCTGGTAGCAATAGCAGGCAATGGCTTCATCACTGCTGCTCTGGGCGTGGAGTGGGT GCTACGGAGAATGTTGTTGCCTTGTGATAAGTTATTGGTTAGCCTAGGGGCCTCTCGCTTCTGTCTGCAG TCAGTGGTAATGGGTAAGACCATTTATGTTTTCTTGCATCCGATGGCCTTCCCATACAACCCTGTACTGC AGTTTCTAGCTTTCCAGTGGGACTTCCTGAATGCTGCCACCTTATGGTCCTCTACCTGGCTCAGTGTCTT CTATTGTGTGAAAATTGCTACCTTCACCCACCCTGTCTTCTTCTGGCTAAAGCACAAGTTGTCTGGGTGG CTACCATGGATGCTCTTCAGCTCTGTAGGGCTCTCCAGCTTCACCACCATTCTATTTTTCATAGGCAACC ACAGAATGTATCAGAACTATTTAAGGAACCATCTACAACCTTGGAATGTCACTGGCGATAGCATACGGAG CTACTGTGAGAAATTCTATCTCTTCCCTCTAAAAATGATTACTTGGACAATGCCCACTGCTGTCTTTTTC ATTTGCATGATTTTGCTCATCACATCTCTGGGAAGACACAGGAAGAAGGCTCTCCTTACAACCTCAGGAT TCCGAGAGCCCAGTGTGCAGGCACACATAAAGGCTCTGCTGGCTCTCCTCTCTTTTGCCATGCTCTTCAT CTCATATTTCCTGTCACTGGTGTTCAGTGCTGCAGGTATTTTTCCACCTCTGGACTTTAAATTCTGGGTG TGGGAGTCAGTGATTTATCTGTGTGCAGCAGTTCACCCCATCATTCTGCTCTTCAGCAACTGCAGGCTGA GAGCTGTGCTGAAGAGTCGTCGTTCCTCAAGGTGTGGGACACCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222067 representing NM_177437
Red=Cloning site Green=Tags(s) MNGDHMVLGSSVTDKKAIILVTILLLLRLVAIAGNGFITAALGVEWVLRRMLLPCDKLLVSLGASRFCLQ SVVMGKTIYVFLHPMAFPYNPVLQFLAFQWDFLNAATLWSSTWLSVFYCVKIATFTHPVFFWLKHKLSGW LPWMLFSSVGLSSFTTILFFIGNHRMYQNYLRNHLQPWNVTGDSIRSYCEKFYLFPLKMITWTMPTAVFF ICMILLITSLGRHRKKALLTTSGFREPSVQAHIKALLALLSFAMLFISYFLSLVFSAAGIFPPLDFKFWV WESVIYLCAAVHPIILLFSNCRLRAVLKSRRSSRCGTP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_177437 |
ORF Size | 954 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_177437.1, NP_803186.1 |
RefSeq Size | 957 bp |
RefSeq ORF | 957 bp |
Locus ID | 338398 |
UniProt ID | P59551 |
Cytogenetics | 7q35 |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Taste transduction |
MW | 36.2 kDa |
Gene Summary | This gene encodes a member of the bitter taste receptor family which belong to the G protein-coupled receptor superfamily and are predominantly expressed in taste receptor cells of the tongue and palate epithelia. This intronless taste receptor gene encodes a seven-transmembrane receptor protein, functioning as a bitter taste receptor. This gene is clustered together with eight other taste receptor genes on chromosome 7. [provided by RefSeq, Jul 2017] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC222067L3 | Lenti ORF clone of Human taste receptor, type 2, member 60 (TAS2R60), Myc-DDK-tagged |
USD 600.00 |
|
RC222067L4 | Lenti ORF clone of Human taste receptor, type 2, member 60 (TAS2R60), mGFP tagged |
USD 600.00 |
|
RG222067 | TAS2R60 (tGFP-tagged) - Human taste receptor, type 2, member 60 (TAS2R60) |
USD 500.00 |
|
SC307094 | TAS2R60 (untagged)-Human taste receptor, type 2, member 60 (TAS2R60) |
USD 330.00 |
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