PNLDC1 (NM_173516) Human Tagged ORF Clone
CAT#: RC221944
PNLDC1 (Myc-DDK-tagged)-Human poly(A)-specific ribonuclease (PARN)-like domain containing 1 (PNLDC1)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_173516" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PNLDC1 |
Synonyms | HsPNLDC1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC221944 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCTGCACCCGAGGACTGCTATTTTTTGCCTTCCTGGCAGGTCTGGACATAGAGTTCACGGGCCTTC GTTCTAACCTGTCTGGGCCCCAGCAGATCAGTCTTTTTGATTTGCCATCGGAGTGGTATCTAAAGACCCG TCAGAGTGTTCAGCAATTTACAGTCTGTCAGATTGGATTGTCTGTGTTTTCCGCTATTGAAGGAGAGGCA AACAAGTATATAGCCCATTCGTGTAACTTCTATCTCTTCCCTACAACGTTTGGGATTTTGGACTCAGAAT TCTCCTTCCAGGCTTCCAGTGTTCAGTTTTTGAATCAGTATGGCTTCAACTATAACAAGTTTCTCAAAAA CGGAATCCCATATATGAATGAAGAACAGGAGAAGAAAATTAGACACGATATCCTGACTGGGAACTGGAGA GTTCGCAGCTCTCCGGATAAAGACCAAATCAAGGTGGTGATTGACGAAGTGACGCGGTGGCTGGAGCTGG CCAAGGAAGGCGACTGGATGACTCTTCCTGGGATCACTGGCTTCCAGGCCTTTGAGGTCCAACTGGTGCT GAGGCAGGCCCTCCCCAACATCTGGACGGTGCTGAAAGATGAGGGGGTGGTAGTGAAGAAAGTGAGTAAA CAACATCGTTGGTATCTTCAGAACACCTCTTGTGACCGAGAGAGCTGTTGGAAGGAAAATATTCTTCTCT CAGCAAGGGGTTTTTCTGTCTTTTTCCAAATGCTGGTGAAAGCCCAGAAGCCCTTAGTGGGACATAATAT GATGATGGACCTGCTGCACCTCCATGAGAAGTTCTTCAGACCCCTCCCAGAAAGCTACGATCAATTTAAG CAGAATATCCACAGCCTATTTCCTGTTCTCATTGATACCAAGAGTGTAACAAAGGATATCTGGAAGGAGA TGAATTTCCCGAGGGTGTCGAATCTTTCGGAAGTCTATGAAGTCCTGAACAGTGACTTGAATCCCACCAA GAATTCTGGACCAGAGATTGTTCACGCGAGCAGGTGTGAGAAATATGTTGAGACAAAGTGCCCCCACGAA GCCGCGTATGATGCCTTCCTCTGTGGGTCAGTTCTTTTGAAAGTGGCACACTTGCTTCTACAGAAGATAT ACCACATCGACCCCGTGCCCGAGTCATCCTTTCCTCAGTACCTTGACGTGCTGGCTCCTTACGTGAACCA AGTGAACCTCATCCGAGCGGGGGTCCCAAAGATCAATTTTTCTGGTCCAGATTATCCCAGTATCCGACCT CCCATCCTCATCCTCAGCGTCAAAAGGTGGCCTGGGGTCAGCGAGCAGCAAGTCTACCATAAGTTTCAGA ATCTCTGCAAGTTTGATGTCAGGCGACTCACAAGAAGCCAGTTCTTACTCCTGACCAATAAGTTTAAGGA TGCGCGGAACATCCTGAAGGAGTACCGGGACCACCCGACCCTGTGCATCTCCCTGTACCGCTACTGGAGG CACTCCCCAAACGTCAACTGCCTGCTCCAAGTCTGTGGCATAGTGACTGCCTGGGCCCTTCTCGCGTTCA TCCTTGGAAGATCTGGTACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221944 protein sequence
Red=Cloning site Green=Tags(s) MFCTRGLLFFAFLAGLDIEFTGLRSNLSGPQQISLFDLPSEWYLKTRQSVQQFTVCQIGLSVFSAIEGEA NKYIAHSCNFYLFPTTFGILDSEFSFQASSVQFLNQYGFNYNKFLKNGIPYMNEEQEKKIRHDILTGNWR VRSSPDKDQIKVVIDEVTRWLELAKEGDWMTLPGITGFQAFEVQLVLRQALPNIWTVLKDEGVVVKKVSK QHRWYLQNTSCDRESCWKENILLSARGFSVFFQMLVKAQKPLVGHNMMMDLLHLHEKFFRPLPESYDQFK QNIHSLFPVLIDTKSVTKDIWKEMNFPRVSNLSEVYEVLNSDLNPTKNSGPEIVHASRCEKYVETKCPHE AAYDAFLCGSVLLKVAHLLLQKIYHIDPVPESSFPQYLDVLAPYVNQVNLIRAGVPKINFSGPDYPSIRP PILILSVKRWPGVSEQQVYHKFQNLCKFDVRRLTRSQFLLLTNKFKDARNILKEYRDHPTLCISLYRYWR HSPNVNCLLQVCGIVTAWALLAFILGRSGT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_173516 |
ORF Size | 1560 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_173516.2, NP_775787.1 |
RefSeq Size | 1958 bp |
RefSeq ORF | 1563 bp |
Locus ID | 154197 |
UniProt ID | Q8NA58 |
Cytogenetics | 6q25.3 |
MW | 60.1 kDa |
Gene Summary | 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails (PubMed:27515512). Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development (PubMed:27515512). May act as a regulator of multipotency in embryonic stem cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC221944L3 | Lenti ORF clone of Human poly(A)-specific ribonuclease (PARN)-like domain containing 1 (PNLDC1), Myc-DDK-tagged |
USD 784.00 |
|
RC221944L4 | Lenti ORF clone of Human poly(A)-specific ribonuclease (PARN)-like domain containing 1 (PNLDC1), mGFP tagged |
USD 784.00 |
|
RG221944 | PNLDC1 (tGFP-tagged) - Human poly(A)-specific ribonuclease (PARN)-like domain containing 1 (PNLDC1) |
USD 684.00 |
|
SC306842 | PNLDC1 (untagged)-Human poly(A)-specific ribonuclease (PARN)-like domain containing 1 (PNLDC1) |
USD 533.00 |
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