PNLDC1 (NM_173516) Human Tagged ORF Clone

CAT#: RC221944

PNLDC1 (Myc-DDK-tagged)-Human poly(A)-specific ribonuclease (PARN)-like domain containing 1 (PNLDC1)

Product Images

Circular map for RC221944

• 

  Western blot validation of overexpression lysate (Cat# LY406448) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221944 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PNLDC1
• Synonyms: HsPNLDC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221944 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTGCACCCGAGGACTGCTATTTTTTGCCTTCCTGGCAGGTCTGGACATAGAGTTCACGGGCCTTC
  GTTCTAACCTGTCTGGGCCCCAGCAGATCAGTCTTTTTGATTTGCCATCGGAGTGGTATCTAAAGACCCG
  TCAGAGTGTTCAGCAATTTACAGTCTGTCAGATTGGATTGTCTGTGTTTTCCGCTATTGAAGGAGAGGCA
  AACAAGTATATAGCCCATTCGTGTAACTTCTATCTCTTCCCTACAACGTTTGGGATTTTGGACTCAGAAT
  TCTCCTTCCAGGCTTCCAGTGTTCAGTTTTTGAATCAGTATGGCTTCAACTATAACAAGTTTCTCAAAAA
  CGGAATCCCATATATGAATGAAGAACAGGAGAAGAAAATTAGACACGATATCCTGACTGGGAACTGGAGA
  GTTCGCAGCTCTCCGGATAAAGACCAAATCAAGGTGGTGATTGACGAAGTGACGCGGTGGCTGGAGCTGG
  CCAAGGAAGGCGACTGGATGACTCTTCCTGGGATCACTGGCTTCCAGGCCTTTGAGGTCCAACTGGTGCT
  GAGGCAGGCCCTCCCCAACATCTGGACGGTGCTGAAAGATGAGGGGGTGGTAGTGAAGAAAGTGAGTAAA
  CAACATCGTTGGTATCTTCAGAACACCTCTTGTGACCGAGAGAGCTGTTGGAAGGAAAATATTCTTCTCT
  CAGCAAGGGGTTTTTCTGTCTTTTTCCAAATGCTGGTGAAAGCCCAGAAGCCCTTAGTGGGACATAATAT
  GATGATGGACCTGCTGCACCTCCATGAGAAGTTCTTCAGACCCCTCCCAGAAAGCTACGATCAATTTAAG
  CAGAATATCCACAGCCTATTTCCTGTTCTCATTGATACCAAGAGTGTAACAAAGGATATCTGGAAGGAGA
  TGAATTTCCCGAGGGTGTCGAATCTTTCGGAAGTCTATGAAGTCCTGAACAGTGACTTGAATCCCACCAA
  GAATTCTGGACCAGAGATTGTTCACGCGAGCAGGTGTGAGAAATATGTTGAGACAAAGTGCCCCCACGAA
  GCCGCGTATGATGCCTTCCTCTGTGGGTCAGTTCTTTTGAAAGTGGCACACTTGCTTCTACAGAAGATAT
  ACCACATCGACCCCGTGCCCGAGTCATCCTTTCCTCAGTACCTTGACGTGCTGGCTCCTTACGTGAACCA
  AGTGAACCTCATCCGAGCGGGGGTCCCAAAGATCAATTTTTCTGGTCCAGATTATCCCAGTATCCGACCT
  CCCATCCTCATCCTCAGCGTCAAAAGGTGGCCTGGGGTCAGCGAGCAGCAAGTCTACCATAAGTTTCAGA
  ATCTCTGCAAGTTTGATGTCAGGCGACTCACAAGAAGCCAGTTCTTACTCCTGACCAATAAGTTTAAGGA
  TGCGCGGAACATCCTGAAGGAGTACCGGGACCACCCGACCCTGTGCATCTCCCTGTACCGCTACTGGAGG
  CACTCCCCAAACGTCAACTGCCTGCTCCAAGTCTGTGGCATAGTGACTGCCTGGGCCCTTCTCGCGTTCA
  TCCTTGGAAGATCTGGTACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221944 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFCTRGLLFFAFLAGLDIEFTGLRSNLSGPQQISLFDLPSEWYLKTRQSVQQFTVCQIGLSVFSAIEGEA
  NKYIAHSCNFYLFPTTFGILDSEFSFQASSVQFLNQYGFNYNKFLKNGIPYMNEEQEKKIRHDILTGNWR
  VRSSPDKDQIKVVIDEVTRWLELAKEGDWMTLPGITGFQAFEVQLVLRQALPNIWTVLKDEGVVVKKVSK
  QHRWYLQNTSCDRESCWKENILLSARGFSVFFQMLVKAQKPLVGHNMMMDLLHLHEKFFRPLPESYDQFK
  QNIHSLFPVLIDTKSVTKDIWKEMNFPRVSNLSEVYEVLNSDLNPTKNSGPEIVHASRCEKYVETKCPHE
  AAYDAFLCGSVLLKVAHLLLQKIYHIDPVPESSFPQYLDVLAPYVNQVNLIRAGVPKINFSGPDYPSIRP
  PILILSVKRWPGVSEQQVYHKFQNLCKFDVRRLTRSQFLLLTNKFKDARNILKEYRDHPTLCISLYRYWR
  HSPNVNCLLQVCGIVTAWALLAFILGRSGT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173516
• ORF Size: 1560 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173516.2, NP_775787.1
• RefSeq Size: 1958 bp
• RefSeq ORF: 1563 bp
• Locus ID: 154197
• UniProt ID: Q8NA58
• Cytogenetics: 6q25.3
• MW: 60.1 kDa
• Gene Summary: 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails (PubMed:27515512). Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development (PubMed:27515512). May act as a regulator of multipotency in embryonic stem cells (By similarity).[UniProtKB/Swiss-Prot Function]