EIF3A (NM_003750) Human Tagged ORF Clone

CAT#: RC221766

EIF3A (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit A (EIF3A)

Product Images

Circular map for RC221766

• 

  Western blot validation of overexpression lysate (Cat# LY401232) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221766 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EIF3A
• Synonyms: EIF3; eIF3-p170; eIF3-theta; EIF3S10; P167; p180; p185; TIF32
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221766 representing NM_003750
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGCCTATTTTCAGAGGCCGGAAAATGCCCTCAAACGCGCCAACGAATTTCTTGAGGTTGGCAAAA
  AGCAGCCTGCTCTGGATGTTCTTTATGATGTTATGAAAAGTAAAAAACATAGAACATGGCAAAAGATACA
  CGAACCAATTATGTTGAAATACTTGGAACTTTGCGTGGATCTTCGCAAGAGCCACTTGGCAAAGGAGGGG
  TTATACCAGTATAAGAACATTTGTCAACAGGTGAACATAAAATCTCTGGAGGATGTTGTTAGGGCATATT
  TGAAAATGGCAGAGGAAAAAACTGAAGCTGCTAAAGAAGAATCTCAGCAGATGGTCTTAGATATAGAGGA
  TCTAGATAATATTCAAACTCCTGAGAGTGTTCTCCTAAGTGCTGTAAGTGGTGAAGACACTCAGGATCGT
  ACTGACAGATTACTTTTAACTCCATGGGTTAAATTCCTGTGGGAGTCTTACAGGCAGTGTTTGGACCTTC
  TTAGAAACAATTCTAGAGTAGAGCGCCTGTACCATGATATTGCCCAGCAAGCTTTCAAATTCTGCCTCCA
  ATACACGCGTAAGGCTGAATTCCGTAAACTGTGTGACAATTTGAGAATGCACTTATCGCAGATTCAGCGC
  CACCATAACCAAAGTACGGCAATCAATCTTAATAATCCAGAGAGCCAGTCCATGCATTTGGAAACCAGAC
  TTGTTCAGCTGGACAGTGCTATCAGCATGGAATTGTGGCAGGAAGCATTCAAAGCTGTGGAAGATATTCA
  CGGGCTATTCTCCTTGTCTAAAAAACCACCTAAACCTCAGTTGATGGCAAATTACTATAACAAAGTCTCA
  ACTGTGTTTTGGAAATCTGGAAATGCTCTTTTTCATGCATCTACACTCCATCGTCTTTACCATCTCTCTA
  GAGAAATGAGAAAGAATCTCACACAAGACGAGATGCAAAGAATGTCTACTAGAGTCCTTTTAGCCACTCT
  TTCCATCCCTATTACTCCTGAGCGTACGGATATTGCTCGACTTCTGGATATGGATGGCATTATAGTTGAA
  AAACAGCGTCGCCTTGCAACACTACTAGGTCTTCAAGCCCCACCGACACGAATTGGCCTTATTAATGATA
  TGGTCAGATTTAATGTACTACAATATGTTGTCCCAGAAGTGAAAGACCTTTACAATTGGCTTGAAGTAGA
  ATTTAACCCATTAAAACTCTGTGAGCGAGTCACAAAGGTTCTAAATTGGGTTAGGGAACAACCTGAAAAG
  GAACCGGAATTGCAGCAGTATGTGCCACAACTGCAAAACAACACCATCCTCCGCCTTCTGCAGCAGGTGT
  CACAGATTTATCAGAGCATTGAGTTTTCTCGTTTGACTTCTTTGGTTCCTTTTGTTGATGCTTTCCAACT
  GGAACGGGCCATAGTAGATGCAGCCAGGCATTGCGACTTGCAGGTTCGTATTGATCACACTTCTCGGACC
  CTGAGTTTTGGATCTGATTTGAATTATGCTACTCGAGAAGATGCTCCGATTGGTCCTCATTTGCAAAGCA
  TGCCTTCAGAGCAGATAAGAAACCAGCTGACAGCCATGTCCTCAGTACTTGCAAAAGCACTTGAAGTCAT
  TAAACCAGCTCATATACTGCAAGAGAAAGAAGAACAGCATCAGTTGGCTGTCACTGCATACCTTAAAAAT
  TCACGAAAAGAGCACCAGCGGATCCTGGCTCGCCGCCAGACAATTGAGGAGAGAAAAGAGCGCCTTGAGA
  GTCTGAATATTCAGCGTGAGAAAGAAGAATTGGAACAGAGGGAAGCTGAACTCCAGAAAGTGCGGAAGGC
  TGAGGAAGAGAGGCTGCGCCAGGAAGCAAAGGAGAGAGAGAAGGAGCGTATCTTACAGGAACATGAACAA
  ATCAAAAAGAAAACTGTCCGAGAGCGTTTGGAGCAGATCAAGAAAACAGAACTGGGTGCCAAAGCATTCA
  AAGATATTGATATTGAAGACCTTGAGGAATTGGATCCAGATTTTATCATGGCTAAACAGGTTGAACAACT
  GGAGAAAGAAAAGAAAGAACTTCAAGAACGCCTAAAGAATCAAGAAAAGAAGATTGACTATTTTGAAAGA
  GCCAAACGTTTGGAAGAAATTCCTTTGATAAAGAGCGCTTACGAGGAACAGAGAATTAAAGACATGGATC
  TGTGGGAGCAACAAGAGGAAGAAAGAATTACTACAATGCAGCTAGAACGTGAAAAGGCTCTTGAACATAA
  GAATCGAATGTCACGAATGCTTGAAGACAGAGATTTATTCGTAATGCGACTCAAAGCTGCACGGCAGTCT
  GTTTATGAGGAAAAACTTAAACAGTTTGAAGAGCGATTAGCAGAAGAAAGGCATAATCGATTGGAAGAAC
  GGAAAAGGCAGCGTAAAGAAGAACGCAGGATAACATACTATAGAGAAAAAGAAGAGGAGGAGCAGAGAAG
  GGCAGAAGAACAAATGCTAAAAGAGCGGGAAGAGAGAGAGCGCGCCGAACGAGCAAAACGCGAGGAAGAG
  CTACGAGAGTATCAGGAGCGGGTGAAGAAATTAGAAGAAGTGGAAAGGAAAAAACGCCAAAGGGAGTTGG
  AAATTGAAGAACGAGAACGGCGTAGAGAGGAAGAGAGAAGACTTGGCGATAGTTCCCTTTCTAGAAAGGA
  CTCTCGTTGGGGAGATAGAGATTCAGAAGGCACCTGGAGAAAAGGACCTGAAGCAGATTCTGAGTGGAGA
  AGAGGCCCGCCAGAGAAGGAGTGGAGACGTGGAGAAGGGCGAGATGAGGACAGGTCTCATAGAAGAGATG
  AAGAGCGGCCCCGGCGTCTGGGGGATGATGAAGATAGAGAGCCCTCTCTTAGACCAGACGATGATCGGGT
  TCCCCGGCGTGGCATGGATGATGACAGAGGCCCTAGACGTGGTCCTGAGGAAGATAGGTTCTCTCGTCGT
  GGGGCAGACGATGACCGGCCTTCCTGGCGTAACACAGATGATGACAGGCCTCCCAGACGAATTGCCGATG
  AAGACAGGGGAAACTGGCGTCATGCGGATGATGACAGACCACCTAGACGAGGACTGGATGAGGACAGAGG
  AAGCTGGCGAACAGCTGATGAGGACAGAGGACCAAGACGTGGGATGGATGATGACCGGGGGCCGAGGCGA
  GGAGGCGCTGATGATGAGCGATCATCCTGGCGTAATGCTGATGATGACCGGGGTCCCAGGCGAGGGTTGG
  ATGATGATCGGGGTCCCAGGCGAGGCATGGATGATGACCGGGGTCCCAGGCGAGGCATGGATGATGACCG
  GGGTCCCAGGCGAGGCATGGATGATGACCGGGGTCCCAGGCGAGGGTTGGATGATGATCGAGGACCTTGG
  AGGAACGCCGATGATGACAGAATTCCCAGGCGTGGTGCAGAGGATGACAGGGGCCCTTGGAGAAACATGG
  ATGATGATCGCCTTTCAAGACGTGCTGATGATGATCGGTTTCCCAGACGGGGTGATGACTCAAGACCTGG
  TCCTTGGAGACCATTAGTCAAGCCAGGTGGATGGAGAGAGAAAGAAAAAGCCAGAGAGGAGAGCTGGGGT
  CCACCTCGAGAATCAAGGCCATCAGAAGAACGTGAATGGGACAGAGAAAAAGAAAGGGACAGAGATAATC
  AAGATCGGGAGGAGAATGACAAGGACCCTGAGAGAGAAAGGGACAGAGAGAGAGATGTGGATCGAGAGGA
  TCGCTTCAGAAGACCTAGGGATGAAGGTGGCTGGAGAAGAGGACCAGCTGAGGAATCTTCAAGCTGGAGA
  GACTCAAGTCGCCGGGACGATAGGGATAGGGATGACCGTCGCCGTGAGAGGGATGACCGGCGTGATCTAA
  GAGAAAGACGAGATCTAAGAGACGACAGGGACCGAAGAGGACCTCCACTCAGATCAGAACGTGAAGAAGT
  AAGTTCTTGGAGACGTGCTGATGACAGGAAAGATGACCGGGTGGAAGAGCGGGACCCTCCTCGTCGAGTT
  CCTCCCCCAGCTCTTTCAAGAGACCGAGAAAGAGACCGAGACCGAGAAAGAGAAGGTGAAAAAGAGAAGG
  CCTCATGGAGAGCTGAGAAAGATAGGGAATCTCTCCGTCGTACTAAAAATGAGACTGATGAAGATGGATG
  GACCACAGTACGACGT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221766 representing NM_003750
  Red=Cloning site Green=Tags(s)
  
  MPAYFQRPENALKRANEFLEVGKKQPALDVLYDVMKSKKHRTWQKIHEPIMLKYLELCVDLRKSHLAKEG
  LYQYKNICQQVNIKSLEDVVRAYLKMAEEKTEAAKEESQQMVLDIEDLDNIQTPESVLLSAVSGEDTQDR
  TDRLLLTPWVKFLWESYRQCLDLLRNNSRVERLYHDIAQQAFKFCLQYTRKAEFRKLCDNLRMHLSQIQR
  HHNQSTAINLNNPESQSMHLETRLVQLDSAISMELWQEAFKAVEDIHGLFSLSKKPPKPQLMANYYNKVS
  TVFWKSGNALFHASTLHRLYHLSREMRKNLTQDEMQRMSTRVLLATLSIPITPERTDIARLLDMDGIIVE
  KQRRLATLLGLQAPPTRIGLINDMVRFNVLQYVVPEVKDLYNWLEVEFNPLKLCERVTKVLNWVREQPEK
  EPELQQYVPQLQNNTILRLLQQVSQIYQSIEFSRLTSLVPFVDAFQLERAIVDAARHCDLQVRIDHTSRT
  LSFGSDLNYATREDAPIGPHLQSMPSEQIRNQLTAMSSVLAKALEVIKPAHILQEKEEQHQLAVTAYLKN
  SRKEHQRILARRQTIEERKERLESLNIQREKEELEQREAELQKVRKAEEERLRQEAKEREKERILQEHEQ
  IKKKTVRERLEQIKKTELGAKAFKDIDIEDLEELDPDFIMAKQVEQLEKEKKELQERLKNQEKKIDYFER
  AKRLEEIPLIKSAYEEQRIKDMDLWEQQEEERITTMQLEREKALEHKNRMSRMLEDRDLFVMRLKAARQS
  VYEEKLKQFEERLAEERHNRLEERKRQRKEERRITYYREKEEEEQRRAEEQMLKEREERERAERAKREEE
  LREYQERVKKLEEVERKKRQRELEIEERERRREEERRLGDSSLSRKDSRWGDRDSEGTWRKGPEADSEWR
  RGPPEKEWRRGEGRDEDRSHRRDEERPRRLGDDEDREPSLRPDDDRVPRRGMDDDRGPRRGPEEDRFSRR
  GADDDRPSWRNTDDDRPPRRIADEDRGNWRHADDDRPPRRGLDEDRGSWRTADEDRGPRRGMDDDRGPRR
  GGADDERSSWRNADDDRGPRRGLDDDRGPRRGMDDDRGPRRGMDDDRGPRRGMDDDRGPRRGLDDDRGPW
  RNADDDRIPRRGAEDDRGPWRNMDDDRLSRRADDDRFPRRGDDSRPGPWRPLVKPGGWREKEKAREESWG
  PPRESRPSEEREWDREKERDRDNQDREENDKDPERERDRERDVDREDRFRRPRDEGGWRRGPAEESSSWR
  DSSRRDDRDRDDRRRERDDRRDLRERRDLRDDRDRRGPPLRSEREEVSSWRRADDRKDDRVEERDPPRRV
  PPPALSRDRERDRDREREGEKEKASWRAEKDRESLRRTKNETDEDGWTTVRR
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003750
• ORF Size: 4146 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003750.1, NP_003741.1
• RefSeq Size: 5256 bp
• RefSeq ORF: 4149 bp
• Locus ID: 8661
• UniProt ID: Q14152
• Cytogenetics: 10q26.11
• Domains: PCI
• MW: 166.4 kDa
• Gene Summary: RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632, PubMed:11169732). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773, PubMed:27462815).[UniProtKB/Swiss-Prot Function]