SEC14L5 (NM_014692) Human Tagged ORF Clone

CAT#: RC220818

SEC14L5 (Myc-DDK-tagged)-Human SEC14-like 5 (S. cerevisiae) (SEC14L5)

Product Images

Circular map for RC220818

• 

  Western blot validation of overexpression lysate (Cat# LY415109) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220818 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SEC14L5
• Synonyms: PRELID4B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220818 representing NM_014692
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCAAAGATACCAGTCTCCTGTCCGAGTCTACAAGTACCCGTTTGAGCTGGTCATGGCGGCCTACG
  AGAAGCGTTTCCCCACGTGCCCACAGATCCCAGTCTTCCTGGGCAGCGAGGTCTTGCGCGAGTCCCGCAG
  CCCGGACGGGGCTGTGCACGTGGTGGAGCGGAGCTGCCGGCTGCGCGTGGACGCCCCGCGGCTGCTGCGG
  AAGATCGCAGGTGTTGAGCACGTGGTCTTCGTGCAGACAAACATCTTGAACTGGAAGGAGAGGACGCTCC
  TCATCGAAGCGCACAATGAGACCTTCGCCAACCGCGTGGTGGTGAACGAGCACTGCAGCTACACGGTCCA
  CCCTGAGAATGAAGACTGGACTTGCTTCGAGCAGTCTGCCTCACTGGACATTCGGTCTTTCTTTGGCTTT
  GAAAATGCCTTGGAGAAGATCGCCATGAAGCAGTACACCGCCAACGTCAAGAGGGGGAAGGAGGTGATTG
  AGCATTACCTGAATGAGCTCATCTCCCAGGGTACCTCGCACATTCCGCGCTGGACGCCTGCCCCAGTCCG
  TGAGGAGGATGCCCGCAACCAGGCTGGACCGAGGGACCCCAGCTCCCTGGAGGCCCACGGGCCCCGTAGC
  ACCCTGGGGCCCGCTCTGGAGGCGGTCAGTATGGACGGGGACAAGCTGGATGCGGACTACATTGAGAGGT
  GCCTGGGCCACCTCACGCCCATGCAGGAGAGCTGCCTGATCCAGCTTCGGCACTGGTTACAGGAGACCCA
  CAAAGGCAAGATTCCCAAAGATGAGCACATCCTTCGGTTCCTGCGGGCTCATGACTTCCACCTGGACAAG
  GCCCGGGAAATGCTGCGCCAGTCCTTGAGCTGGCGCAAGCAGCACCAGGTGGATCTCCTCCTTCAGACCT
  GGCAACCCCCTGCCCTGCTGGAGGAGTTCTATGCAGGGGGCTGGCATTACCAGGACATAGATGGCCGCCC
  CCTCTACATCCTCCGCCTGGGCCAGATGGACACCAAAGGCTTGATGAAGGCCGTGGGGGAGGAGGCGCTG
  CTGCGGCATGTTCTCTCCGTCAACGAGGAAGGACAGAAGCGGTGTGAGGGGAGCACAAGGCAGCTGGGCC
  GTCCCATCAGCTCCTGGACCTGCCTGCTAGACCTGGAGGGACTCAACATGCGGCACCTGTGGCGGCCGGG
  GGTGAAGGCCCTGCTGCGGATGATTGAGGTGGTTGAGGACAATTACCCAGAGACCCTGGGTCGGCTGCTC
  ATCGTGCGAGCCCCCCGAGTCTTCCCCGTGCTCTGGACACTGATCAGCCCCTTCATCAATGAGAACACCA
  GGCGGAAGTTCCTCATCTACAGTGGCAGCAACTACCAGGGACCCGGAGGCCTTGTGGACTATCTGGATAG
  AGAAGTGATCCCTGACTTCCTTGGGGGAGAGAGTGTGTGTAATGTCCCCGAAGGAGGGCTGGTCCCCAAG
  TCCCTCTACATGACAGAAGAGGAGCAGGAGCACACGGACCAGCTGTGGCAGTGGAGTGAGACCTACCATT
  CAGCCAGCGTGCTCCGCGGAGCCCCCCACGAGGTGGCCGTGGAGATCCTGGAAGGAGAGTCGGTCATCAC
  CTGGGACTTTGACATCCTGCGAGGGGACGTGGTGTTCAGCCTGTACCACACCAAGCAGGCGCCCAGGCTG
  GGCGCCCGGGAACCGGGGACCAGGGCCAGCGGGCAGCTGATCGACAAAGGCTGGGTCCTGGGCAGGGATT
  ACAGCCGTGTGGAGGCTCCCCTTGTCTGCCGGGAGGGGGAGAGCATCCAGGGCTCCCATGTGACCCGGTG
  GCCCGGCGTCTACCTGCTCCAGTGGCAAATGCACAGCCCCCCCAGCAGCGTGGCCTGCAGCCTCCCGGGT
  GTGGACGATGTCCTGACGGCTCTGCACAGCCCCGGGCCCAAGTGCAAACTTCTCTACTACTGTGAGGTGC
  TCGCCTCTGAGGACTTCAGGGGCTCCATGTCCAGCCTGGAATCCTGCACCAGCGGCTTCTCCCAGCTCAG
  CGCCGCCACCTCGTCCTCCTCCTCCGGCCAGTCTCATAGCAGCTCCCTGGTCTCCAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220818 representing NM_014692
  Red=Cloning site Green=Tags(s)
  
  MVQRYQSPVRVYKYPFELVMAAYEKRFPTCPQIPVFLGSEVLRESRSPDGAVHVVERSCRLRVDAPRLLR
  KIAGVEHVVFVQTNILNWKERTLLIEAHNETFANRVVVNEHCSYTVHPENEDWTCFEQSASLDIRSFFGF
  ENALEKIAMKQYTANVKRGKEVIEHYLNELISQGTSHIPRWTPAPVREEDARNQAGPRDPSSLEAHGPRS
  TLGPALEAVSMDGDKLDADYIERCLGHLTPMQESCLIQLRHWLQETHKGKIPKDEHILRFLRAHDFHLDK
  AREMLRQSLSWRKQHQVDLLLQTWQPPALLEEFYAGGWHYQDIDGRPLYILRLGQMDTKGLMKAVGEEAL
  LRHVLSVNEEGQKRCEGSTRQLGRPISSWTCLLDLEGLNMRHLWRPGVKALLRMIEVVEDNYPETLGRLL
  IVRAPRVFPVLWTLISPFINENTRRKFLIYSGSNYQGPGGLVDYLDREVIPDFLGGESVCNVPEGGLVPK
  SLYMTEEEQEHTDQLWQWSETYHSASVLRGAPHEVAVEILEGESVITWDFDILRGDVVFSLYHTKQAPRL
  GAREPGTRASGQLIDKGWVLGRDYSRVEAPLVCREGESIQGSHVTRWPGVYLLQWQMHSPPSSVACSLPG
  VDDVLTALHSPGPKCKLLYYCEVLASEDFRGSMSSLESCTSGFSQLSAATSSSSSGQSHSSSLVSR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014692
• ORF Size: 2088 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014692.2
• RefSeq Size: 6456 bp
• RefSeq ORF: 2091 bp
• Locus ID: 9717
• UniProt ID: O43304
• Cytogenetics: 16p13.3
• MW: 78.8 kDa