Cytokeratin 4 (KRT4) (NM_002272) Human Tagged ORF Clone

CAT#: RC220350

KRT4 (Myc-DDK-tagged)-Human keratin 4 (KRT4)

Product Images

Circular map for RC220350

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  Western blot validation of overexpression lysate (Cat# LY419424) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220350 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  HEK293T cells were transfected with the overexpression plasmids of 14 KRT isoforms (from left to right:KRT1, Cat# RC223146; KRT2, Cat# RC213030; KRT3, Cat# RC223011; KRT4, Cat# RC220350; KRT5, Cat# RC206870; KRT6a, Cat# RC204328; KRT7, Cat# RC201124; KRT8, Cat# RC209570;KRT9, Cat# RC218091; KRT10, Cat# RC204500;KRT12, Cat# RC224946;KRT13, Cat# RC201179;KRT14, Cat# RC214907;KRT15, Cat# RC201150) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-flag antibody (Cat# TA180144, 1:1000) or anti-KRT7 mouse monoclonal antibody (Cat# TA803333, 1:500).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cytokeratin 4
• Synonyms: CK-4; CK4; CYK4; K4; WSN1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220350 representing NM_002272
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTCTGTAGGTGTGTTTAGTGACATGCTCAACGGGTGCGGGAAGGATGGGCTTGTGCCAAGGGCCA
  AGCCCAGAGATGTTTCAGATTTTTCCCTTTATGCCCCTGCAACCAAGCCCTGCTGCTCCAGGACATATAA
  GAGACGAAGGCTGAGGGCTCCAGCACTCACCGGCCTGGGCCCTGTCACTTCTCTGATAGCTCCCAGCTCG
  CTCTCTGCAGCCATGATTGCCAGACAGCAGTGTGTCCGAGGCGGGCCCCGGGGCTTCAGCTGTGGCTCGG
  CCATTGTAGGCGGTGGCAAGAGAGGTGCCTTCAGCTCAGTCTCCATGTCTGGAGGTGCTGGCCGATGCTC
  TTCTGGGGGATTTGGCAGCAGAAGCCTCTACAACCTCAGGGGGAACAAAAGCATCTCCATGAGTGTGGCT
  GGGTCACGACAAGGTGCCTGCTTTGGGGGTGCTGGAGGCTTTGGCACTGGTGGCTTTGGTGGTGGATTTG
  GGGGCTCCTTCAGTGGTAAGGGTGGCCCTGGCTTCCCCGTCTGCCCCGCTGGGGGAATTCAGGAGGTCAC
  CATCAACCAGAGCTTGCTCACCCCCCTCCACGTGGAGATTGACCCTGAGATCCAGAAAGTCCGGACGGAA
  GAGCGCGAACAGATCAAGCTCCTCAACAACAAGTTTGCCTCCTTCATCGACAAGGTGCAGTTCTTAGAGC
  AACAGAATAAGGTCCTGGAGACCAAATGGAACCTGCTCCAGCAGCAGACGACCACCACCTCCAGCAAAAA
  CCTTGAGCCCCTCTTTGAGACCTACCTCAGTGTCCTGAGGAAGCAGCTAGATACCTTGGGCAATGACAAA
  GGGCGCCTGCAGTCTGAGCTGAAGACCATGCAGGACAGCGTGGAGGACTTCAAGACTAAGTATGAAGAGG
  AGATCAACAAACGCACAGCAGCCGAGAATGACTTTGTGGTCCTAAAGAAGGACGTGGATGCTGCCTACCT
  GAACAAGGTGGAGTTGGAGGCCAAGGTGGACAGTCTTAATGACGAGATCAACTTCCTGAAGGTCCTCTAT
  GATGCGGAGCTGTCCCAGATGCAGACCCATGTCAGCGACACGTCCGTGGTCCTTTCCATGGACAACAACC
  GCAACCTGGACCTGGACAGCATTATTGCCGAGGTCCGTGCCCAGTACGAGGAGATTGCCCAGAGGAGCAA
  GGCTGAGGCTGAAGCCCTGTACCAGACCAAGGTCCAGCAGCTCCAGATCTCGGTTGACCAACATGGTGAC
  AACCTGAAGAACACCAAGAGTGAAATTGCAGAGCTCAACAGGATGATCCAGAGGCTGCGGGCAGAGATCG
  AGAACATCAAGAAGCAGTGCCAGACTCTTCAGGTATCCGTGGCTGATGCAGAGCAGCGAGGTGAGAATGC
  CCTTAAAGATGCCCACAGCAAGCGCGTAGAGCTGGAGGCTGCCCTGCAGCAGGCCAAGGAGGAGCTGGCA
  CGAATGCTGCGTGAGTACCAGGAGCTCATGAGTGTGAAGCTGGCCTTGGACATCGAGATCGCCACCTACC
  GCAAACTGCTGGAGGGCGAGGAGTACAGAATGTCTGGAGAATGCCAGAGTGCCGTGAGCATCTCTGTGGT
  CAGCGGTAGCACCAGCACTGGAGGCATCAGCGGAGGATTAGGAAGTGGCTCCGGGTTTGGCCTGAGTAGT
  GGCTTTGGCTCCGGCTCTGGAAGTGGCTTTGGGTTTGGTGGCAGTGTCTCTGGCAGTTCCAGCAGCAAGA
  TCATCTCTACCACCACCCTGAACAAGAGACGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220350 representing NM_002272
  Red=Cloning site Green=Tags(s)
  
  MTSVGVFSDMLNGCGKDGLVPRAKPRDVSDFSLYAPATKPCCSRTYKRRRLRAPALTGLGPVTSLIAPSS
  LSAAMIARQQCVRGGPRGFSCGSAIVGGGKRGAFSSVSMSGGAGRCSSGGFGSRSLYNLRGNKSISMSVA
  GSRQGACFGGAGGFGTGGFGGGFGGSFSGKGGPGFPVCPAGGIQEVTINQSLLTPLHVEIDPEIQKVRTE
  EREQIKLLNNKFASFIDKVQFLEQQNKVLETKWNLLQQQTTTTSSKNLEPLFETYLSVLRKQLDTLGNDK
  GRLQSELKTMQDSVEDFKTKYEEEINKRTAAENDFVVLKKDVDAAYLNKVELEAKVDSLNDEINFLKVLY
  DAELSQMQTHVSDTSVVLSMDNNRNLDLDSIIAEVRAQYEEIAQRSKAEAEALYQTKVQQLQISVDQHGD
  NLKNTKSEIAELNRMIQRLRAEIENIKKQCQTLQVSVADAEQRGENALKDAHSKRVELEAALQQAKEELA
  RMLREYQELMSVKLALDIEIATYRKLLEGEEYRMSGECQSAVSISVVSGSTSTGGISGGLGSGSGFGLSS
  GFGSGSGSGFGFGGSVSGSSSSKIISTTTLNKRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002272
• ORF Size: 1782 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002272.2, NP_002263.2
• RefSeq Size: 2576 bp
• RefSeq ORF: 1563 bp
• Locus ID: 3851
• UniProt ID: P19013
• Cytogenetics: 12q13.13
• Domains: filament
• MW: 63.7 kDa
• Gene Summary: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in differentiated layers of the mucosal and esophageal epithelia with family member KRT13. Mutations in these genes have been associated with White Sponge Nevus, characterized by oral, esophageal, and anal leukoplakia. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]