ZNF614 (NM_025040) Human Tagged ORF Clone

CAT#: RC220077

ZNF614 (Myc-DDK-tagged)-Human zinc finger protein 614 (ZNF614)

Product Images

Circular map for RC220077

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF614
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220077 representing NM_025040
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCAAGACCCAGGAATCACTGACCCTGGAGGATGTGGCTGTGGAATTCAGCTGGGAGGAGTGGCAGC
  TCCTGGACACTGCTCAGAAGAACCTGTACCGGGATGTGATGGTGGAGAACTATAACCACCTAGTATCACT
  GGGGTATCAAACTAGCAAACCAGATGTACTCTCCAAGTTGGCACATGGACAAGAACCATGGACAACAGAT
  GCTAAAATTCAGAATAAAAATTGTCCAGGAATCGGGAAAGTTGACAGTCATCTGCAAGAGCACTCTCCAA
  ACCAAAGACTTCTGAAGAGCGTGCAGCAATGCAATGGACAGAATACACTTAGAAATATTGTACATCTCAG
  CAAGACACATTTTCCTATAGTGCAAAATCATGATACATTTGACTTGTACAGAAAAAATTTGAAATCAAGT
  TTAAGTTTAATCAACCAGAAGAGAAGACATGGAATAAATAACCCTGTTGAGTTTATTGGAGGTGAGAAAA
  CACTTCTACATGGTAAGCATGAACGTACGCATACTAAAACTAGATTTTCTGAAAATGCAAAATGTATCCA
  TACTAAATTCCAAGTCTTCAAGCATCAGAGGACTCAGAAAATTGAGAAACCCCATGCATGCATTGAATGT
  GAGCAAACCTTCCTTAGGAAGTCTCAGCTCATTTACCATGAGAACATTTGTATACAAGAGAATCCTGGAA
  GTGGTCAATGTGAGAAATTATCCAGAAGTGTCCTGTTCACTAAGCATCTGAAAACTAATACAACAGACAA
  AATCTGTATACCCAATGAATATAGAAAAGGCTCTACTGTGAAGAGTAGTCTCATTACACATCAACAAACC
  CATACAGAAGAGAAATCCTATATGTGCAGTGAGTGTGGAAAGGGCTTTACAATGAAGCGCTATCTAATTG
  CTCATCAGCGAACTCATAGTGGAGAGAAACCTTATGTGTGCAAAGAATGTGGAAAAGGTTTCACTGTGAA
  GAGCAATCTCATTGTACATCAGCGAACTCATACAGGGGAGAAACCCTATATATGCAGTGAATGTGGAAAA
  GGCTTCACCATGAAGCGCTATCTTGTTGTACATCAGCGAACTCATACTGGAGAGAAACCCTATATGTGCA
  GTGAATGTGGAAAAGGCTTTACCGTGAAGAGCAATCTCATTGTACATCAGCGCTCCCACACAGGAGAAAA
  ATCTTACATATGCAGCGAATGTGGAAAAGGCTTCACTGTCAAACGCACTCTCGTTATACATCAGCGAACT
  CATACAGGAGAGAAATCTTACATATGCAATGAATGTGGTAAAGGCTTCACCACAAAGCGCACTCTTATTA
  TACATCAGCGAACTCATACAGGAGAAAAACCCTATGAATGCAATGAATGTGGTAAAGCCTTCAGCCAGAA
  AATATGCCTCATACAACATGAGAGATGTCATACAGGAAAGACTCCCTTTGTATGTACCGAGTGCGGAAAA
  TCCTATTCACACAAATATGGCCTCATTACCCATCAGAGAATTCACACAGGAGAGAAACCTTATGAGTGCA
  ATGAATGTGGAAAAGCCTTCACCACAAAGTCAGTACTCAATGTACATCAAAGAACGCATACAGGAGAGAG
  GCCGTATGGATGCAGTGATTGTGAGAAAGCCTTCTCCCACTTATCAAACCTTGTCAAACATAAGAAAATG
  CACACAAGAGAAATGGGTAGAATCAGTCAAGTTGAAAACTCCTGTAATGGAGAGTCACAGCTCCTTCCTT
  ATAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220077 representing NM_025040
  Red=Cloning site Green=Tags(s)
  
  MIKTQESLTLEDVAVEFSWEEWQLLDTAQKNLYRDVMVENYNHLVSLGYQTSKPDVLSKLAHGQEPWTTD
  AKIQNKNCPGIGKVDSHLQEHSPNQRLLKSVQQCNGQNTLRNIVHLSKTHFPIVQNHDTFDLYRKNLKSS
  LSLINQKRRHGINNPVEFIGGEKTLLHGKHERTHTKTRFSENAKCIHTKFQVFKHQRTQKIEKPHACIEC
  EQTFLRKSQLIYHENICIQENPGSGQCEKLSRSVLFTKHLKTNTTDKICIPNEYRKGSTVKSSLITHQQT
  HTEEKSYMCSECGKGFTMKRYLIAHQRTHSGEKPYVCKECGKGFTVKSNLIVHQRTHTGEKPYICSECGK
  GFTMKRYLVVHQRTHTGEKPYMCSECGKGFTVKSNLIVHQRSHTGEKSYICSECGKGFTVKRTLVIHQRT
  HTGEKSYICNECGKGFTTKRTLIIHQRTHTGEKPYECNECGKAFSQKICLIQHERCHTGKTPFVCTECGK
  SYSHKYGLITHQRIHTGEKPYECNECGKAFTTKSVLNVHQRTHTGERPYGCSDCEKAFSHLSNLVKHKKM
  HTREMGRISQVENSCNGESQLLPYK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025040
• ORF Size: 1755 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025040.4
• RefSeq Size: 4627 bp
• RefSeq ORF: 1758 bp
• Locus ID: 80110
• UniProt ID: Q8N883
• Cytogenetics: 19q13.41
• Protein Families: Transcription Factors
• MW: 67 kDa
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]