PCDHA13 (NM_018904) Human Tagged ORF Clone

CAT#: RC219904

PCDHA13 (Myc-DDK-tagged)-Human protocadherin alpha 13 (PCDHA13), transcript variant 1

Product Images

Circular map for RC219904

• 

  Western blot validation of overexpression lysate (Cat# LY412916) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219904 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PCDHA13
• Synonyms: CNR5; CNRN5; CNRS5; CRNR5; PCDH-ALPHA13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219904 representing NM_018904
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTCTTCCTGGCAAGGAGGCCCAAGACCGCGGCAACTACTGCTCTGGCTTCTGATCCTCGCAGCCT
  GGGAGACGGGTAGTGGCCAGCTCCACTACTCCGTCCCCGAGGAAGCAAAACACGGCACCTTCGTGGGCCG
  CATCGCTCAGGACCTGGGGCTGGAGCTGGCGGAGCTGGTGCCGCGCCTGTTCCGGGTGGCGTCCAAAAGA
  CACGGGGACCTTCTGGAGGTAAATCTGCAGAATGGCATTTTGTTTGTGAATTCTCGGATCGACCGCGAGG
  AGCTGTGTGGGCGGAGCGCGGAGTGCAGCATCCACCTGGAGGTGATCGTGGACAGGCCTCTGCAGGTTTT
  CCATGTGGAGGTGAAGGTGAGGGACATTAACGACAACCCGCCCATATTCCCTGAAAGCAAGAAACGAATA
  ATCATTGCAGAATCTAGACCTCCGGAAACTCGATTTCCACTAGATGGCGCATCCGATGCAGATATTGGAG
  TAAACTCGGCATTGACCTACCGACTGGATCCCAACGATTATTTCACTTTGGACGCACAAAACAGTCTTGA
  GCAAATGTCTTCATTATCACTTGTACTGAGGAAAACACTGGACAGAGAGGAAATTCAGGAACATAGTTTA
  TTACTGACAGCCAGTGATGGAGGTAAACCCGAGCTGACTGGCACAGTTCAGCTGCTCATCACGATTCTGG
  ACGTGAATGACAACGCCCCGGAATTTTACCAATCCGTTTATAAAGTGACGGTGTTAGAGAACGCCTTCAA
  TGGAACATTAGTGATCAAGCTAAATGCCACAGATCCTGATGATGGTACAAATGGAGATATAGTTTACTCA
  TTTAGAAGGCCTGTATGGCCTGCAGTGGTATATGCATTTACCATAAATCCGAACAATGGAGAAATTAGGA
  CAAAAGGCAAACTAGATTTCGAAGAAAAGAAATTATATGAAATATCCGTGGAGGCAGTTGACAAAGGAAA
  TATTCCAATGGCGGGTCATTGTACCCTTTTGGTGGAAGTACTAGATGTAAATGATAACGCCCCAGAGGTT
  ACCATCACTTCTTTGTCACTCCCCATCAGAGAAGACACTCAGCCTAGCGCCATTATTGCCCTAATCAGTG
  TGTCCGATCGTGACTCTGGCTCAAATGGACAGGTCACCTGCACCTTGACGCCGCATGTCCCCTTCAAGCT
  GGTGTCCACCTACAAGAACTACTACTCATTAGTGCTGGACAGCGCCCTGGACCGCGAGAGCGTATCAGCC
  TATGAACTGGTGGTGACCGCGCGGGACGGGGGCTCGCCTTCGCTGTGGGCCACGGCCAGCGTGTCGGTGG
  GGGTGGCCGACGTGAACGACAACGCGCCGGCGTTCGCGCAGCCCGAGTACACGGTGTTCGTGAAGGAAAA
  CAATCCGCCGGGCTGCCACATCTTCACGGTGTCTGCTCAGGACGCGGACGCACAGGAGAACGCGCTGGTC
  TCCTACTCGCTGGTGGAGCGGCGGGTGGGCGAGCGTGCGCTGTCGAGCTACGTGTCGGTGCACGCGGAGA
  GCGGCAAGGTGTACGCGCTGCAGCCGTTGGACCACGAGGAGCTGGAGCTGTTGCAGTTCCAGGTGAGCGC
  GCGCGACTCTGGCGTGCCGCCTCTGGGCAGCAACGTGACGCTGCAGGTGTTCGTGCTGGACGAGAACGAC
  AACGCTCCGGCGCTGCTGACGCCCGGGGCTGGCAGCGCGGGAGGCACAGTGAGCGAGCTGATGCCGCGGT
  CGGTGGGTGCAGGCCACGTGGTGGCGAAGGTGCGCGCGGTGGACGCCGATTCGGGCTACAATGCGTGGCT
  TTCGTATGAATTGCAGCTGGCGGCGGTCGGCGCGCGCATCCCGTTCCGCGTGGGGCTGTACACTGGCGAG
  ATCAGCACGACGCGCCCTCTGGACGAGGTGGACGCGCCGCACCACCGCCTTCTGGTGCTGGTGAAGGACC
  ACGGTGAGCCCGCGCTGACGGCCACGGCAACGGTGCTGTTGTCGCTGGTGGAGAGCGGCCAAGCGCCACA
  GGCTTCGTCGAGGGCGTCGGCAGGCGCTGTGGGTCCAGAAGCGGCGCTGGTGGATGTCAATGTTTACTTG
  ATCATTGCCATCTGCGCGGTGTCCAGCCTGTTGGTGCTCACGTTGCTGCTGTATACTGCGCTGCGGTGCT
  CGGCACCGCCCACCGAGGGCGCGTGCGCGCCGGGCAAGCCCACTCTAGTGTGCTCCAGCGCGGCAGGGAG
  TTGGTCGTACTCGCAGCAGAGGCGGCCGAGGGTGTGCTCTGGGGAGGGCCCGCATAAGACGGACCTCATG
  GCCTTCAGTCCCAGCCTTCCTCCTTGTCTGGGTTCTGCAGAGGGAACAGGCCAGAGGGAGGAGGACTCAG
  AATGCTTGAAAGAGCCACGACAGCCCAACCCTGACTGGCGTTACTCTGCCTCCCTGAGAGCAGGCATGCA
  CAGCTCTGTGCACCTAGAGGAGGCTGGCATTCTACGGGCTGGTCCAGGAGGGCCTGATCAGCAGTGGCCA
  ACAGTATCCAGTGCAACACCAGAACCAGAGGCAGGAGAAGTGTCCCCTCCAGTCGGTGCGGGTGTCAACA
  GCAACAGCTGGACCTTTAAATACGGACCAGGCAACCCCAAACAATCCGGTCCCGGTGAGTTGCCCGACAA
  ATTCATTATCCCAGGATCTCCTGCAATCATCTCCATCCGGCAGGAGCCTACTAACAGCCAAATTGACAAA
  AGTGACTTCATAACCTTCGGCAAAAAGGAGGAGACCAAGAAAAAGAAGAAAAAGAAGAAGGGTAACAAGA
  CCCAGGAGAAAAAAGAGAAAGGGAACAGCACGACTGACAACAGTGACCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219904 representing NM_018904
  Red=Cloning site Green=Tags(s)
  
  MLSSWQGGPRPRQLLLWLLILAAWETGSGQLHYSVPEEAKHGTFVGRIAQDLGLELAELVPRLFRVASKR
  HGDLLEVNLQNGILFVNSRIDREELCGRSAECSIHLEVIVDRPLQVFHVEVKVRDINDNPPIFPESKKRI
  IIAESRPPETRFPLDGASDADIGVNSALTYRLDPNDYFTLDAQNSLEQMSSLSLVLRKTLDREEIQEHSL
  LLTASDGGKPELTGTVQLLITILDVNDNAPEFYQSVYKVTVLENAFNGTLVIKLNATDPDDGTNGDIVYS
  FRRPVWPAVVYAFTINPNNGEIRTKGKLDFEEKKLYEISVEAVDKGNIPMAGHCTLLVEVLDVNDNAPEV
  TITSLSLPIREDTQPSAIIALISVSDRDSGSNGQVTCTLTPHVPFKLVSTYKNYYSLVLDSALDRESVSA
  YELVVTARDGGSPSLWATASVSVGVADVNDNAPAFAQPEYTVFVKENNPPGCHIFTVSAQDADAQENALV
  SYSLVERRVGERALSSYVSVHAESGKVYALQPLDHEELELLQFQVSARDSGVPPLGSNVTLQVFVLDEND
  NAPALLTPGAGSAGGTVSELMPRSVGAGHVVAKVRAVDADSGYNAWLSYELQLAAVGARIPFRVGLYTGE
  ISTTRPLDEVDAPHHRLLVLVKDHGEPALTATATVLLSLVESGQAPQASSRASAGAVGPEAALVDVNVYL
  IIAICAVSSLLVLTLLLYTALRCSAPPTEGACAPGKPTLVCSSAAGSWSYSQQRRPRVCSGEGPHKTDLM
  AFSPSLPPCLGSAEGTGQREEDSECLKEPRQPNPDWRYSASLRAGMHSSVHLEEAGILRAGPGGPDQQWP
  TVSSATPEPEAGEVSPPVGAGVNSNSWTFKYGPGNPKQSGPGELPDKFIIPGSPAIISIRQEPTNSQIDK
  SDFITFGKKEETKKKKKKKKGNKTQEKKEKGNSTTDNSDQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018904
• ORF Size: 2850 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018904.3
• RefSeq Size: 5260 bp
• RefSeq ORF: 2853 bp
• Locus ID: 56136
• UniProt ID: Q9Y5I0
• Cytogenetics: 5q31.3
• MW: 99.2 kDa
• Gene Summary: This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined. [provided by RefSeq, Jul 2008]