SAP155 (SF3B1) (NM_012433) Human Tagged ORF Clone

CAT#: RC219605

SF3B1 (Myc-DDK-tagged)-Human splicing factor 3b, subunit 1, 155kDa (SF3B1), transcript variant 1. Note: ORF is codon optimized

Product Images

Circular map for RC219605

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SAP155
• Synonyms: Hsh155; MDS; PRP10; PRPF10; SAP155; SF3b155
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219605 ORF sequence, codon optimized.
  Due to the complexity of NM_012433, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAAGATCGCCAAGACTCACGAAGATATTGAAGCACAGATTCGAGAAATTCAAGGCAAGAAGGCAG
  CTCTTGATGAAGCTCAAGGAGTGGGCCTCGATTCTACAGGTTATTATGACCAGGAAATTTATGGTGGAAG
  TGACAGCAGATTTGCTGGATACGTGACATCAATTGCTGCAACTGAACTTGAAGATGATGACGATGACTAT
  TCATCATCTACGAGTTTGCTTGGTCAGAAGAAGCCAGGATATCATGCCCCTGTGGCATTGCTTAATGATA
  TACCACAGTCAACAGAACAGTATGATCCATTTGCTGAGCACAGACCTCCAAAGATTGCAGACCGGGAAGA
  TGAATACAAAAAGCATAGGCGGACCATGATAATTTCCCCAGAGCGTCTTGATCCTTTTGCAGATGGAGGG
  AAAACCCCTGATCCTAAAATGAATGCTAGGACTTACATGGATGTAATGCGAGAACAACACTTGACTAAAG
  AAGAACGAGAAATTAGGCAACAGCTAGCAGAAAAAGCTAAAGCTGGAGAACTAAAAGTCGTCAATGGAGC
  AGCAGCGTCCCAGCCTCCATCAAAACGAAAACGGCGTTGGGATCAAACAGCTGATCAGACTCCTGGTGCC
  ACTCCCAAAAAACTATCAAGTTGGGATCAGGCAGAGACCCCTGGGCATACTCCTTCCTTAAGATGGGATG
  AGACACCAGGTCGTGCAAAGGGAAGCGAGACTCCTGGAGCAACCCCAGGCTCAAAAATATGGGATCCTAC
  ACCTAGCCACACACCAGCGGGAGCTGCTACTCCTGGACGAGGTGATACACCAGGCCATGCGACACCAGGC
  CATGGAGGCGCAACTTCCAGTGCTCGTAAAAACAGATGGGATGAAACCCCCAAAACAGAGAGAGATACTC
  CTGGGCATGGAAGTGGATGGGCTGAGACTCCTCGAACAGATCGAGGTGGAGATTCTATTGGTGAAACACC
  GACTCCTGGAGCCAGTAAAAGAAAATCACGGTGGGATGAAACACCAGCTAGTCAGATGGGTGGAAGCACT
  CCAGTTCTGACCCCTGGAAAGACACCAATTGGCACACCAGCCATGAACATGGCTACCCCTACTCCAGGTC
  ACATAATGAGTATGACTCCTGAACAGCTTCAGGCTTGGCGGTGGGAAAGAGAAATTGATGAGAGAAATCG
  CCCACTTTCTGATGAGGAATTAGATGCTATGTTCCCAGAAGGATATAAGGTACTTCCTCCTCCAGCTGGT
  TATGTTCCTATTCGAACTCCAGCTCGAAAGCTGACAGCTACTCCAACACCTTTGGGTGGTATGACTGGTT
  TCCACATGCAAACTGAAGATCGAACTATGAAAAGTGTTAATGACCAGCCATCTGGAAATCTTCCATTTTT
  AAAACCTGATGATATTCAATACTTTGATAAACTATTGGTTGATGTTGATGAATCAACACTTAGTCCAGAA
  GAGCAAAAAGAGAGAAAAATAATGAAGTTGCTTTTAAAAATTAAGAATGGAACACCACCAATGAGAAAGG
  CTGCATTGCGTCAGATTACTGATAAAGCTCGTGAATTTGGAGCTGGTCCTTTGTTTAATCAGATTCTTCC
  TCTGCTGATGTCTCCTACACTTGAGGATCAAGAGCGTCATTTACTTGTGAAAGTTATTGATAGGATACTG
  TACAAACTTGATGACTTAGTTCGTCCATATGTGCATAAGATCCTCGTGGTCATTGAACCGCTATTGATTG
  ATGAAGATTACTATGCTAGAGTGGAAGGCCGAGAGATCATTTCTAATTTGGCAAAGGCTGCTGGTCTGGC
  TACTATGATCTCTACCATGAGACCTGATATAGATAACATGGATGAGTATGTCCGTAACACAACAGCTAGA
  GCTTTTGCTGTTGTAGCCTCTGCCCTGGGCATTCCTTCTTTATTGCCCTTCTTAAAAGCTGTGTGCAAAA
  GCAAGAAGTCCTGGCAAGCGAGACACACTGGTATTAAGATTGTACAACAGATAGCTATTCTTATGGGCTG
  TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCTTGTGGATGAGCAGCAGAAA
  GTTCGGACCATCAGTGCTTTGGCCATTGCTGCCTTGGCTGAAGCAGCAACTCCTTATGGTATCGAATCTT
  TTGATTCTGTGTTAAAGCCTTTATGGAAGGGTATCCGCCAACACAGAGGAAAGGGTTTGGCTGCTTTCTT
  GAAGGCTATTGGGTATCTTATTCCTCTTATGGATGCAGAATATGCCAACTACTATACTAGAGAAGTGATG
  TTAATCCTTATTCGAGAATTCCAGTCTCCTGATGAGGAAATGAAAAAAATTGTGCTGAAGGTGGTAAAAC
  AGTGTTGTGGGACAGATGGTGTAGAAGCAAACTACATTAAAACAGAGATTCTTCCTCCCTTTTTTAAACA
  CTTCTGGCAGCACAGGATGGCTTTGGATAGAAGAAATTACCGACAGTTAGTTGATACTACTGTGGAGTTG
  GCAAACAAAGTAGGTGCAGCAGAAATTATATCCAGGATTGTGGATGATCTGAAAGATGAAGCCGAACAGT
  ACAGAAAAATGGTGATGGAGACAATTGAGAAGATTATGGGGAACCTCGGGGCGGCAGACATTGATCATAA
  GCTCGAAGAACAGCTGATCGACGGGATCCTGTATGCATTTCAGGAGCAGACCACGGAAGACTCAGTGATG
  CTTAACGGCTTTGGCACTGTCGTAAACGCCCTTGGCAAACGGGTCAAACCCTATCTTCCGCAGATCTGTG
  GCACTGTGCTGTGGAGGTTGAATAACAAATCTGCCAAAGTTAGACAGCAGGCAGCCGATCTCATTTCTCG
  GACCGCAGTGGTTATGAAGACATGTCAGGAGGAAAAGTTGATGGGACATTTGGGTGTAGTGCTCTACGAA
  TACTTGGGGGAAGAATATCCCGAAGTGCTCGGCTCCATCCTCGGCGCGCTGAAGGCAATCGTAAATGTTA
  TTGGGATGCATAAGATGACGCCACCTATCAAGGACCTCTTGCCGCGCCTGACCCCGATCCTCAAGAATAG
  GCATGAGAAGGTACAGGAAAACTGTATCGACCTGGTGGGAAGAATCGCTGATCGGGGAGCCGAATACGTC
  TCAGCCCGGGAGTGGATGAGAATTTGCTTCGAACTGCTGGAGCTGCTGAAGGCCCATAAGAAGGCAATTC
  GGCGCGCCACGGTTAATACCTTTGGCTACATAGCTAAGGCCATCGGGCCTCACGACGTCCTCGCAACCCT
  GCTGAACAATCTGAAAGTTCAGGAACGGCAGAACAGGGTGTGTACGACCGTGGCCATTGCCATTGTGGCT
  GAAACGTGTAGCCCCTTCACCGTCCTGCCCGCCCTCATGAACGAGTACCGCGTCCCGGAGTTGAACGTGC
  AGAACGGGGTCCTCAAGAGTCTGAGCTTTTTGTTCGAGTATATCGGAGAGATGGGCAAAGACTACATTTA
  CGCAGTGACCCCACTTCTGGAAGACGCCCTGATGGACCGGGATCTGGTCCACAGGCAGACCGCTTCCGCT
  GTTGTCCAGCATATGAGTCTCGGAGTTTATGGATTCGGCTGTGAGGACAGTCTGAATCATTTGCTTAACT
  ATGTATGGCCCAACGTGTTTGAAACTTCCCCTCATGTTATTCAGGCCGTGATGGGAGCTCTTGAGGGGCT
  CCGGGTAGCAATCGGCCCCTGCCGGATGCTGCAGTACTGCCTCCAGGGGCTCTTCCATCCTGCAAGGAAA
  GTGAGAGATGTTTATTGGAAGATCTACAACAGTATTTACATAGGGTCCCAAGACGCTTTGATTGCTCACT
  ACCCTCGGATCTACAATGACGACAAGAATACTTACATTAGGTACGAACTGGACTACATTCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219605 representing NM_012433
  Red=Cloning site Green=Tags(s)
  
  MAKIAKTHEDIEAQIREIQGKKAALDEAQGVGLDSTGYYDQEIYGGSDSRFAGYVTSIAATELEDDDDDY
  SSSTSLLGQKKPGYHAPVALLNDIPQSTEQYDPFAEHRPPKIADREDEYKKHRRTMIISPERLDPFADGG
  KTPDPKMNARTYMDVMREQHLTKEEREIRQQLAEKAKAGELKVVNGAAASQPPSKRKRRWDQTADQTPGA
  TPKKLSSWDQAETPGHTPSLRWDETPGRAKGSETPGATPGSKIWDPTPSHTPAGAATPGRGDTPGHATPG
  HGGATSSARKNRWDETPKTERDTPGHGSGWAETPRTDRGGDSIGETPTPGASKRKSRWDETPASQMGGST
  PVLTPGKTPIGTPAMNMATPTPGHIMSMTPEQLQAWRWEREIDERNRPLSDEELDAMFPEGYKVLPPPAG
  YVPIRTPARKLTATPTPLGGMTGFHMQTEDRTMKSVNDQPSGNLPFLKPDDIQYFDKLLVDVDESTLSPE
  EQKERKIMKLLLKIKNGTPPMRKAALRQITDKAREFGAGPLFNQILPLLMSPTLEDQERHLLVKVIDRIL
  YKLDDLVRPYVHKILVVIEPLLIDEDYYARVEGREIISNLAKAAGLATMISTMRPDIDNMDEYVRNTTAR
  AFAVVASALGIPSLLPFLKAVCKSKKSWQARHTGIKIVQQIAILMGCAILPHLRSLVEIIEHGLVDEQQK
  VRTISALAIAALAEAATPYGIESFDSVLKPLWKGIRQHRGKGLAAFLKAIGYLIPLMDAEYANYYTREVM
  LILIREFQSPDEEMKKIVLKVVKQCCGTDGVEANYIKTEILPPFFKHFWQHRMALDRRNYRQLVDTTVEL
  ANKVGAAEIISRIVDDLKDEAEQYRKMVMETIEKIMGNLGAADIDHKLEEQLIDGILYAFQEQTTEDSVM
  LNGFGTVVNALGKRVKPYLPQICGTVLWRLNNKSAKVRQQAADLISRTAVVMKTCQEEKLMGHLGVVLYE
  YLGEEYPEVLGSILGALKAIVNVIGMHKMTPPIKDLLPRLTPILKNRHEKVQENCIDLVGRIADRGAEYV
  SAREWMRICFELLELLKAHKKAIRRATVNTFGYIAKAIGPHDVLATLLNNLKVQERQNRVCTTVAIAIVA
  ETCSPFTVLPALMNEYRVPELNVQNGVLKSLSFLFEYIGEMGKDYIYAVTPLLEDALMDRDLVHRQTASA
  VVQHMSLGVYGFGCEDSLNHLLNYVWPNVFETSPHVIQAVMGALEGLRVAIGPCRMLQYCLQGLFHPARK
  VRDVYWKIYNSIYIGSQDALIAHYPRIYNDDKNTYIRYELDYIL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012433
• ORF Size: 3912 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012433.2, NP_036565.2
• RefSeq Size: 4338 bp
• RefSeq ORF: 3915 bp
• Locus ID: 23451
• UniProt ID: O75533
• Cytogenetics: 2q33.1
• Protein Pathways: Spliceosome
• MW: 145.8 kDa
• Gene Summary: This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]