UBN1 (NM_016936) Human Tagged ORF Clone

CAT#: RC218917

UBN1 (Myc-DDK-tagged)-Human ubinuclein 1 (UBN1), transcript variant 1

Product Images

Circular map for RC218917

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UBN1
• Synonyms: VT; VT4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218917 representing NM_016936
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGAGCCCCACAGGGTCCAGTTCACCTCTCTCCCAGGTTCCCTGAATCCTGCGTTTTTGAAGAAGT
  CCCGGAAGGAGGAGGCTGGGGCAGGAGAACAGCATCAGGACTGTGAGCCGGCTGCAGCAGCTGTTCGGAT
  TACACTCACCCTCTTTGAACCAGATCACAAACGCTGCCCAGAGTTCTTCTACCCAGAGCTGGTGAAGAAT
  ATCCGAGGGAAGGTAAAAGGCCTTCAGCCTGGAGATAAGAAGAAAGATCTGTCAGATCCTTTCAATGACG
  AAGAAAAGGAAAGGCATAAAGTAGAGGCCCTTGCCCGAAAATTTGAAGAAAAATACGGTGGAAAGAAACG
  TAGAAAAGACCGAATACAGGACTTGATCGATATGGGGTATGGTTATGATGAATCCGACTCCTTCATCGAT
  AACTCTGAGGCGTATGATGAGCTTGTTCCTGCTTCTTTGACTACGAAGTATGGAGGATTTTACATTAACT
  CGGGAACCCTGCAGTTTAGACAAGCATCAGAGTCTGAAGATGACTTCATTAAAGAAAAGAAGAAAAAATC
  TCCAAAGAAGCGGAAGTTGAAGGAAGGTGGTGAGAAGATAAAGAAGAAGAAAAAAGATGACACTTATGAC
  AAGGAGAAGAAATCGAAAAAGTCCAAGTTTTCCAAAGCCGGCTTCACAGCCCTCAATGCCAGTAAGGAGA
  AGAAGAAGAAGAAATATTCTGGGGCTTTAAGCGTTAAAGAGATGCTAAAGAAATTTCAGAAAGAGAAAGA
  GGCTCAGAAAAAAAGGGAGGAGGAGCATAAGCCTGTTGCGGTCCCATCAGCGGAAGCTCAGGGCCTGCGG
  GAACTGGAGGGTGCCTCTGACCCCTTGCTCTCACTCTTTGGCTCTACTTCTGACAACGACTTGCTCCAGG
  CGGCCACTGCCATGGACTCGCTGACGGATTTGGACTTGGAGCATCTGCTCAGTGAGTCTCCAGAAGGAAG
  TCCCTTCCGAGATATGGATGATGGAAGTGATTCCCTTGGGGTGGGATTGGACCAGGAATTCAGGCAGCCC
  TCTTCTCTCCCCGAAGGCCTGCCAGCACCCCTGGAGAAGCGCGTTAAGGAGCTGGCTCAGGCTGCCAGAG
  CTGCTGAGGGGGAGAGCAGACAGAAGTTCTTCACCCAGGATATTAATGGAATCCTATTAGACATAGAGGC
  GCAGACTCGGGAGCTGAGCAGTCAGGTCCGCTCTGGGGTGTATGCCTATCTTGCGTCATTCCTGCCCTGC
  AGCAAGGATGCCCTGCTCAAGCGTGCTCGGAAACTTCACCTCTATGAACAGGGGGGCCGTCTGAAGGAGC
  CTCTCCAGAAGCTCAAGGAAGCCATTGGCAGGGCGATGCCAGAGCAGATGGCCAAGTACCAGGACGAATG
  CCAGGCACACACGCAGGCAAAGGTTGCCAAGATGCTGGAAGAGGAGAAAGACAAGGAGCAGAGGGACCGG
  ATTTGTTCGGATGAGGAAGAAGATGAAGAAAAAGGGGGCAGGAGGATAATGGGACCTCGGAAGAAGTTCC
  AATGGAATGATGAGATCAGGGAGCTACTGTGCCAGGTGGTGAAGATCAAACTGGAGAGCCAGGACCTGGA
  GAGGAACAACAAAGCCCAGGCTTGGGAGGACTGTGTGAAGGGCTTTCTGGATGCGGAAGTCAAGCCCCTC
  TGGCCCAAAGGCTGGATGCAGGCCAGAACTCTGTTTAAGGAGAGCAGACGAGGCCATGGGCACCTGACTT
  CAATCCTGGCCAAGAAGAAAGTTATGGCCCCTTCTAAAATCAAGGTGAAGGAATCGTCTACGAAGCCTGA
  TAAAAAGGTTTCTGTCCCATCAGGACAGATTGGTGGCCCCATTGCTTTGCCCTCAGATCACCAAACAGGA
  GGCCTGAGTATTGGGGCCTCGAGCAGGGAGCTCCCATCCCAGGCATCGGGCGGCCTTGCTAACCCTCCTC
  CTGTCAACCTGGAGGACTCATTGGATGAAGACTTGATCCGCAATCCAGCCTCCTCGGTGGAAGCCGTGTC
  CAAGGAATTGGCTGCATTGAATAGCAGAGCAGCTGGGAACTCTGAATTCACACTGCCTGCACCCTCAAAA
  GCACCTGCAGAAAAAGTTGGAGGCGTTTTATGTACAGAAGAAAAAAGGAACTTTGCGAAGCCTAGTCCTT
  CTGCTCCACCACCAGCTAGCTCTCTGCAGTCACCCCTCAATTTTCTGGCAGAACAGGCTCTGGCACTGGG
  GCAGTCCTCTCAGGAGAAAAAACCAGAGAGTTCTGGCTACAAAGAGCTGTCCTGCCAGGCTCCCCTCAAT
  AAGGGCCTGCCAGAAGTACATCAGTCCAAAGCTAAGCACCACAGCTTGCCACGGACGTCTCACGGGCCCC
  AAGTGGCAGTTCCTGTGCCTGGCCCCCAGGTCAAAGTCTTTCATGCCGGCACTCAGCAGCAGAAAAACTT
  CACGCCCCCATCTCCATTTGCCAATAAGCTCCAAGGCCCAAAGGCTTCTCCCACACAGTGTCATCGTTCC
  CTCCTGCAGTTAGTGAAGACAGCGGCCAAAGGCCAGGGCTTCCATCCCTCTGCACCAGCCACCTCAGGAG
  GCCTGTCAGCCTCCAGCAGCAGCTCTCACAAGACCCCAGCCTCGTCCTCTTCTGCCCTGAGCCATCCAGC
  AAAGCCACATTCAGTCAGCTCTGCAGGCTCATCTTACAAGAATAATCCCTTTGCCAGCTCAATCTCCAAA
  CATGGGGTTTCTTCTGGCAGCTCTTCCTCGGGAGGAACACCAGTCCAGAGTTCTGTTTCTGGGAGCCTGG
  TCCCTGGCATACAGCCTCCCTCCGTGGGACAGGCCACCAGCCGACCCGTCCCAAGTTCAGCAGGGAAAAA
  AATGCCTGTTTCCCAGAAGTTGACTCTGGTAGCCCCTCCAGGCGGTCCAAACGGAGATTCCAGTGGTGGG
  ACCCAGGGAGTGGCAAAGTTGCTGACCTCGCCGTCCCTAAAGCCCTCTGCAGTTAGTAGTGTGACATCGT
  CTACCTCCTTGTCAAAAGGAGCGAGTGGGACTGTGCTGCTGGCCGGCTCCTCTTTGATGGCTTCACCCTA
  CAAATCCAGCAGCCCAAAGCTGTCTGGGGCCATGAGCTCGAACTCCTTGGGAATTATAACCCCTGTCCCT
  ATTCCTGTCCATGTGCTCTCCTTCAGCGCTGACTCCTCTGCCAAAGCAGGGGTCTCCAAGGATGCCATCG
  TCACAGGCCCTGCCCCCGGGTCCTTCCACCATGGCCTTGGCCACAGTCTTCTGGCTGGCTTGCACTCCAG
  CCCGCCCCATGCAGCGCCTCTCCCACACGCTGCGGTGCCCACCCATATCCCGCAGAGTCTGCCAGGTGCT
  TCTCAGCTTCACGGGAAAGGGCCTGCTGTACCACGGAAATTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218917 representing NM_016936
  Red=Cloning site Green=Tags(s)
  
  MSEPHRVQFTSLPGSLNPAFLKKSRKEEAGAGEQHQDCEPAAAAVRITLTLFEPDHKRCPEFFYPELVKN
  IRGKVKGLQPGDKKKDLSDPFNDEEKERHKVEALARKFEEKYGGKKRRKDRIQDLIDMGYGYDESDSFID
  NSEAYDELVPASLTTKYGGFYINSGTLQFRQASESEDDFIKEKKKKSPKKRKLKEGGEKIKKKKKDDTYD
  KEKKSKKSKFSKAGFTALNASKEKKKKKYSGALSVKEMLKKFQKEKEAQKKREEEHKPVAVPSAEAQGLR
  ELEGASDPLLSLFGSTSDNDLLQAATAMDSLTDLDLEHLLSESPEGSPFRDMDDGSDSLGVGLDQEFRQP
  SSLPEGLPAPLEKRVKELAQAARAAEGESRQKFFTQDINGILLDIEAQTRELSSQVRSGVYAYLASFLPC
  SKDALLKRARKLHLYEQGGRLKEPLQKLKEAIGRAMPEQMAKYQDECQAHTQAKVAKMLEEEKDKEQRDR
  ICSDEEEDEEKGGRRIMGPRKKFQWNDEIRELLCQVVKIKLESQDLERNNKAQAWEDCVKGFLDAEVKPL
  WPKGWMQARTLFKESRRGHGHLTSILAKKKVMAPSKIKVKESSTKPDKKVSVPSGQIGGPIALPSDHQTG
  GLSIGASSRELPSQASGGLANPPPVNLEDSLDEDLIRNPASSVEAVSKELAALNSRAAGNSEFTLPAPSK
  APAEKVGGVLCTEEKRNFAKPSPSAPPPASSLQSPLNFLAEQALALGQSSQEKKPESSGYKELSCQAPLN
  KGLPEVHQSKAKHHSLPRTSHGPQVAVPVPGPQVKVFHAGTQQQKNFTPPSPFANKLQGPKASPTQCHRS
  LLQLVKTAAKGQGFHPSAPATSGGLSASSSSSHKTPASSSSALSHPAKPHSVSSAGSSYKNNPFASSISK
  HGVSSGSSSSGGTPVQSSVSGSLVPGIQPPSVGQATSRPVPSSAGKKMPVSQKLTLVAPPGGPNGDSSGG
  TQGVAKLLTSPSLKPSAVSSVTSSTSLSKGASGTVLLAGSSLMASPYKSSSPKLSGAMSSNSLGIITPVP
  IPVHVLSFSADSSAKAGVSKDAIVTGPAPGSFHHGLGHSLLAGLHSSPPHAAPLPHAAVPTHIPQSLPGA
  SQLHGKGPAVPRKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016936
• ORF Size: 3402 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016936.3, NP_058632.2
• RefSeq Size: 6485 bp
• RefSeq ORF: 3404 bp
• Locus ID: 29855
• Cytogenetics: 16p13.3
• MW: 121.5 kDa
• Gene Summary: Cellular senescence is a hallmark of tumor suppression and tissue aging. Senescent cells contain domains of heterochromatin, called senescence-associated heterochromatin foci (SAHF), that repress proliferation-promoting genes. The protein encoded by this gene binds to proliferation-promoting genes and is required for SAHF formation, enhancing methylation of histone H3. [provided by RefSeq, Oct 2016]