Transportin 1 (TNPO1) (NM_002270) Human Tagged ORF Clone

CAT#: RC218884

TNPO1 (Myc-DDK-tagged)-Human transportin 1 (TNPO1), transcript variant 1

Product Images

Circular map for RC218884

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Transportin 1
• Synonyms: IPO2; KPNB2; MIP; MIP1; TRN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218884 representing NM_002270
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTGGGACCGGCAAACCAAGATGGAGTATGAGTGGAAACCTGACGAGCAAGGGCTTCAGCAAATCC
  TGCAGCTGTTGAAGGAGTCCCAGTCCCCAGACACCACCATCCAGAGAACCGTGCAACAAAAACTGGAACA
  ACTTAATCAGTATCCAGACTTTAACAACTACTTGATTTTTGTTCTTACAAAATTAAAATCTGAAGATGAA
  CCCACAAGATCATTGAGTGGTCTTATCTTGAAGAATAATGTGAAAGCACACTTTCAGAACTTCCCAAATG
  GTGTAACAGACTTTATTAAAAGTGAATGTTTAAATAATATTGGTGACTCCTCTCCTCTGATTAGAGCCAC
  TGTTGGTATTTTGATCACAACTATAGCCTCCAAGGGAGAATTGCAGAATTGGCCTGACCTCTTACCAAAA
  CTCTGTAGCCTGTTGGATTCTGAAGATTATAATACCTGTGAGGGAGCATTTGGTGCCCTTCAGAAGATTT
  GTGAAGATTCTGCTGAGATTTTAGACAGTGATGTTTTAGATCGTCCTCTCAACATCATGATTCCCAAATT
  TTTACAGTTCTTCAAGCATAGTAGTCCAAAAATAAGGTCTCACGCTGTTGCATGTGTCAATCAGTTTATC
  ATCAGTAGGACTCAAGCTCTAATGTTGCACATTGATTCTTTTATTGAGAATCTCTTTGCATTAGCTGGTG
  ATGAAGAACCAGAGGTACGGAAAAATGTGTGCCGAGCACTTGTGATGTTGCTCGAAGTTCGAATGGATCG
  CCTGCTTCCTCACATGCATAATATAGTTGAGTACATGCTACAGAGGACTCAAGATCAAGATGAAAATGTG
  GCTTTAGAAGCCTGTGAATTTTGGCTAACTTTAGCTGAACAGCCAATATGCAAAGATGTACTCGTAAGGC
  ATCTTCCTAAGTTGATTCCTGTGTTAGTGAATGGCATGAAGTACTCAGACATAGATATTATCCTACTTAA
  GGGTGATGTTGAAGAAGACGAAACGATTCCTGATAGTGAACAGGATATACGGCCACGTTTTCACCGATCG
  AGGACGGTGGCTCAGCAGCATGATGAAGATGGAATTGAAGAGGAAGATGATGATGATGATGAAATTGATG
  ATGATGATACAATTTCTGACTGGAATCTAAGAAAATGTTCTGCTGCTGCCCTGGATGTTCTTGCAAATGT
  GTATCGTGATGAACTGCTGCCACATATTTTGCCCCTTTTGAAAGAATTACTTTTTCATCATGAATGGGTT
  GTTAAAGAATCAGGCATTTTGGTTTTAGGAGCAATTGCTGAAGGTTGCATGCAGGGCATGATTCCATACT
  TGCCTGAGCTTATTCCTCACCTTATTCAGTGCCTCTCTGATAAAAAGGCTCTTGTGCGTTCCATAACATG
  CTGGACTCTTAGCCGCTATGCACACTGGGTGGTCAGCCAGCCGCCAGACACGTACCTGAAGCCATTAATG
  ACAGAATTGCTAAAGCGCATCCTGGACAGCAACAAGAGAGTACAAGAAGCTGCCTGCAGTGCCTTTGCTA
  CCCTAGAAGAGGAGGCTTGTACAGAACTTGTTCCTTACCTTGCTTATATACTTGATACCCTGGTCTTTGC
  ATTTAGTAAATACCAGCATAAGAACCTGCTCATTCTTTACGATGCCATAGGAACATTAGCAGATTCAGTA
  GGACATCATTTAAACAAACCAGAATATATTCAGATGCTAATGCCTCCACTGATCCAGAAATGGAACATGT
  TAAAGGATGAAGATAAAGATCTCTTCCCTTTACTTGAGTGCCTATCTTCAGTTGCCACAGCACTGCAGTC
  TGGATTCCTTCCGTACTGTGAACCTGTGTATCAGCGTTGTGTAAACCTAGTACAGAAGACTCTTGCACAA
  GCCATGCTAAACAATGCTCAACCAGATCAATATGAAGCTCCAGATAAAGATTTTATGATAGTGGCTCTTG
  ATTTACTGAGTGGCCTGGCTGAAGGACTTGGAGGCAACATTGAACAGCTGGTAGCCCGAAGTAACATCCT
  GACACTAATGTATCAGTGCATGCAGGATAAAATGCCAGAAGTTCGACAGAGTTCTTTTGCCCTGTTAGGT
  GACCTCACAAAAGCTTGCTTTCAGCATGTTAAGCCTTGTATAGCTGATTTCATGCCAATATTGGGAACCA
  ACCTAAATCCAGAATTCATTTCAGTCTGCAACAATGCCACATGGGCAATTGGAGAAATCTCCATTCAAAT
  GGGTATAGAGATGCAGCCTTATATTCCTATGGTGTTGCACCAGCTTGTAGAAATCATTAACAGACCCAAC
  ACACCAAAGACGTTGTTAGAGAATACAGCAATAACAATTGGTCGTCTTGGTTACGTTTGTCCTCAAGAGG
  TGGCCCCCATGCTACAGCAGTTTATAAGACCCTGGTGCACCTCTCTGAGAAACATAAGAGACAATGAGGA
  AAAGGATTCAGCATTCCGTGGAATTTGTACCATGATCAGTGTGAATCCCAGTGGCGTAATCCAAGATTTT
  ATATTTTTTTGTGATGCCGTTGCATCATGGATTAACCCAAAAGATGATCTCAGAGACATGTTCTGTAAGA
  TCCTTCATGGATTTAAAAATCAAGTTGGCGATGAAAATTGGAGGCGTTTCTCTGACCAGTTTCCTCTTCC
  CTTAAAAGAGCGTCTTGCAGCTTTTTATGGTGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218884 representing NM_002270
  Red=Cloning site Green=Tags(s)
  
  MVWDRQTKMEYEWKPDEQGLQQILQLLKESQSPDTTIQRTVQQKLEQLNQYPDFNNYLIFVLTKLKSEDE
  PTRSLSGLILKNNVKAHFQNFPNGVTDFIKSECLNNIGDSSPLIRATVGILITTIASKGELQNWPDLLPK
  LCSLLDSEDYNTCEGAFGALQKICEDSAEILDSDVLDRPLNIMIPKFLQFFKHSSPKIRSHAVACVNQFI
  ISRTQALMLHIDSFIENLFALAGDEEPEVRKNVCRALVMLLEVRMDRLLPHMHNIVEYMLQRTQDQDENV
  ALEACEFWLTLAEQPICKDVLVRHLPKLIPVLVNGMKYSDIDIILLKGDVEEDETIPDSEQDIRPRFHRS
  RTVAQQHDEDGIEEEDDDDDEIDDDDTISDWNLRKCSAAALDVLANVYRDELLPHILPLLKELLFHHEWV
  VKESGILVLGAIAEGCMQGMIPYLPELIPHLIQCLSDKKALVRSITCWTLSRYAHWVVSQPPDTYLKPLM
  TELLKRILDSNKRVQEAACSAFATLEEEACTELVPYLAYILDTLVFAFSKYQHKNLLILYDAIGTLADSV
  GHHLNKPEYIQMLMPPLIQKWNMLKDEDKDLFPLLECLSSVATALQSGFLPYCEPVYQRCVNLVQKTLAQ
  AMLNNAQPDQYEAPDKDFMIVALDLLSGLAEGLGGNIEQLVARSNILTLMYQCMQDKMPEVRQSSFALLG
  DLTKACFQHVKPCIADFMPILGTNLNPEFISVCNNATWAIGEISIQMGIEMQPYIPMVLHQLVEIINRPN
  TPKTLLENTAITIGRLGYVCPQEVAPMLQQFIRPWCTSLRNIRDNEEKDSAFRGICTMISVNPSGVIQDF
  IFFCDAVASWINPKDDLRDMFCKILHGFKNQVGDENWRRFSDQFPLPLKERLAAFYGV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002270
• ORF Size: 2694 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002270.4
• RefSeq Size: 8559 bp
• RefSeq ORF: 2697 bp
• Locus ID: 3842
• UniProt ID: Q92973
• Cytogenetics: 5q13.2
• Domains: Armadillo_seg, IBN_NT
• Protein Families: Druggable Genome
• MW: 102.2 kDa
• Gene Summary: This gene encodes the beta subunit of the karyopherin receptor complex which interacts with nuclear localization signals to target nuclear proteins to the nucleus. The karyopherin receptor complex is a heterodimer of an alpha subunit which recognizes the nuclear localization signal and a beta subunit which docks the complex at nucleoporins. Alternate splicing of this gene results in several transcript variants encoding different proteins. [provided by RefSeq, Jun 2018]