HPS5 (NM_181507) Human Tagged ORF Clone

CAT#: RC218495

HPS5 (Myc-DDK-tagged)-Human Hermansky-Pudlak syndrome 5 (HPS5), transcript variant 1

Product Images

Circular map for RC218495

• 

  Western blot validation of overexpression lysate (Cat# LY405678) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218495 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HPS5
• Synonyms: AIBP63; BLOC2S2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218495 representing NM_181507
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTTGTGCCAGTGATACCAGAGTCCTACAGCCATGTTCTTGCAGAGTTTGAATCTCTGGATCCAT
  TACTCTCAGCCCTGCGGCTGGACTCCAGTCGTCTAAAGTGCACGAGCATAGCTGTGTCTCGGAAATGGTT
  GGCTTTGGGCAGTTCAGGAGGAGGACTCCATCTCATTCAGAAAGAAGGCTGGAAGCACAGGCTTTTTCTT
  TCACACAGGGAAGGTGCAATTTCTCAAGTCGCCTGTTGTTTACATGATGATGATTATGTTGCTGTAGCTA
  CCAGTCAAGGTCTTGTGGTTGTTTGGGAATTAAATCAAGAGCGTCGTGGGAAACCGGAACAAATGTATGT
  GTCTTCAGAACACAAAGGCCGAAGAGTCACAGCTCTCTGCTGGGATACAGCTATTCTTAGAGTTTTTGTA
  GGTGATCATGCTGGGAAGGTTTCTGCTATCAAACTCAATACTTCTAAACAAGCAAAGGCAGCTGCTGCTT
  TTGTGATGTTTCCTGTTCAGACAATCACAACTGTTGACTCCTGTGTTGTACAGTTAGATTATTTGGATGG
  AAGGCTACTTATATCTTCACTTACTCGATCCTTCTTGTGTGACACTGAGAGAGAAAAGTTTTGGAAAATT
  GGAAACAAGGAAAGAGATGGAGAATATGGAGCTTGTTTCTTTCCTGGAAGATGTTCTGGGGGCCAGCAAC
  CTCTGATATATTGTGCTCGCCCAGGCTCTAGGATGTGGGAAGTGAACTTTGATGGAGAAGTTATAAGTAC
  ACATCAGTTCAAGAAACTCCTCTCGTTGCCACCTCTCCCTGTGATTACTCTCAGATCAGAACCTCAGTAT
  GATCATACAGCTGGATCCTCCCAGTCTTTGTCTTTCCCCAAACTCTTACATCTTAGTGAGCATTGTGTGC
  TGACTTGGACAGAAAGAGGAATTTATATTTTCATTCCTCAGAATGTTCAAGTTCTTCTTTGGAGTGAAGT
  CAAAGATATTCAGGATGTGGCTGTCTGTAGGAATGAATTGTTCTGTTTGCACCTAAATGGGAAAGTCTCA
  CATCTCTCCCTGATATCTGTGGAGCGCTGTGTGGAACGCCTGCTAAGAAGAGGCCTATGGAACTTGGCTG
  CTCGTACATGCTGTCTTTTCCAAAATTCTGTCATTGCCAGCAGAGCAAGAAAAACTTTGACTGCAGATAA
  ATTGGAGCATTTGAAATCTCAGCTGGACCATGGCACCTACAATGATCTAATTTCTCAACTGGAAGAATTG
  ATCTTAAAATTTGAACCTTTGGATTCAGCTTGTAGCAGTAGAAGAAGCTCCATTTCATCACATGAAAGTT
  TCAGCATCTTGGACTCTGGTATTTATCGTATCATTAGTAGTAGAAGAGGCAGTCAGTCAGATGAAGACTC
  TTGCTCCCTTCACAGCCAAACCCTCTCAGAAGATGAGAGATTTAAAGAATTCACCTCACAGCAGGAAGAG
  GACCTGCCAGATCAGTGTTGTGGCTCACACGGAAATGAAGACAATGTTTCTCATGCTCCAGTGATGTTTG
  AGACAGATAAGAATGAAACTTTTCTCCCGTTCGGCATTCCATTACCATTTCGTTCTCCATCTCCTCTTGT
  GTCTCTTCAGGCTGTCAAAGAAAGTGTTTCTAGCTTTGTGCGTAAAACTACTGAGAAGATTGGCACCCTT
  CACACGAGCCCTGATCTGAAAGTGAGACCAGAGCTCAGGGGTGATGAGCAATCATGTGAAGAGGATGTGA
  GTTCAGATACCTGCCCAAAGGAGGAAGACACTGAGGAGGAAAAAGAGGTAACTAGTCCACCTCCAGAAGA
  AGACAGGTTCCAGGAGCTTAAAGTAGCAACAGCAGAAGCAATGACCAAGCTACAGGACCCTCTGGTTTTA
  TTTGAATCCGAGTCTCTGAGAATGGTTTTACAGGAGTGGCTTTCACATTTAGAAAAAACATTTGCCATGA
  AGGACTTTTCAGGTGTTTCAGATACTGACAACTCATCCATGAAATTGAACCAGGATGTGCTATTAGTTAA
  TGAATCAAAAAAGGGAATATTAGATGAAGATAATGAAAAAGAAAAAAGGGACTCTTTAGGCAATGAAGAA
  TCTGTTGATAAAACAGCATGTGAATGTGTAAGGAGTCCAAGGGAGTCTTTGGATGACCTGTTTCAAATAT
  GTTCTCCATGCGCCATTGCAAGTGGTCTTCGGAACGACCTGGCTGAATTGACAACATTATGTTTGGAGTT
  GAATGTATTGAATTCTAAGATCAAAAGCACCAGTGGACATGTGGACCACACTTTGCAACAGTACTCTCCT
  GAAATTCTGGCTTGCCAGTTCCTGAAGAAGTACTTTTTTCTCCTGAACTTGAAAAGAGCGAAGGAGAGTA
  TCAAGCTTAGTTACAGTAATAGCCCTTCTGTTTGGGATACTTTTATTGAAGGATTGAAAGAAATGGCAAG
  TTCCAATCCTGTGTATATGGAGATGGAAAAAGGAGATCTACCAACAAGGTTAAAGTTACTAGATGACGAG
  GTTCCTTTTGATAGTCCGTTGTTGGTTGTTTATGCTACCCGGTTGTATGAAAAGTTTGGGGAGTCTGCTC
  TTCGATCCTTAATCAAGTTCTTTCCATCCATTTTGCCATCGGATATCATACAACTTTGTCATCATCATCC
  TGCTGAGTTTTTGGCCTATTTAGACAGTCTGGTGAAATCAAGGCCTGAAGATCAGCGGTCATCTTTTCTT
  GAGTCCCTTCTGCAACCAGAGTCTTTAAGGTTGGATTGGCTGCTTTTGGCAGTGTCCCTTGATGCTCCAC
  CAAGCACCAGCACAATGGATGATGAAGGTTATCCCAGGCCTCATTCACACTTGCTTTCCTGGGGTTACAG
  TCAGCTGATCCTTCATCTAATTAAACTTCCTGCAGATTTTATAACCAAAGAGAAAATGACAGACATCTGC
  AGGTCTTGTGGTTTCTGGCCTGGATATCTAATTCTCTGTTTGGAGCTGGAGAGAAGAAGAGAGGCCTTCA
  CCAATATTGTGTATCTGAATGATATGAGCCTGATGGAAGGGGACAATGGTTGGATCCCAGAGACCGTGGA
  GGAATGGAAGCTTCTCCTTCATCTCATACAGAGCAAGAGCACGAGGCCAGCCCCCCAGGAGTCACTAAAT
  GGGAGCCTCAGTGATGGGCCTTCCCCCATCAATGTGGAGAATGTGGCACTTCTGTTAGCTAAGGCCATGG
  GCCCAGATCGGGCTTGGTCACTGCTACAGGAATGTGGTCTGGCCCTTGAGTTGTCAGAGAAGTTTACCAG
  AACCTGCGATATCCTGAGGATTGCTGAGAAAAGGCAGAGGGCCTTGATACAAAGCATGCTTGAAAAATGC
  GATCGGTTTCTCTGGTCCCAGCAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218495 representing NM_181507
  Red=Cloning site Green=Tags(s)
  
  MAFVPVIPESYSHVLAEFESLDPLLSALRLDSSRLKCTSIAVSRKWLALGSSGGGLHLIQKEGWKHRLFL
  SHREGAISQVACCLHDDDYVAVATSQGLVVVWELNQERRGKPEQMYVSSEHKGRRVTALCWDTAILRVFV
  GDHAGKVSAIKLNTSKQAKAAAAFVMFPVQTITTVDSCVVQLDYLDGRLLISSLTRSFLCDTEREKFWKI
  GNKERDGEYGACFFPGRCSGGQQPLIYCARPGSRMWEVNFDGEVISTHQFKKLLSLPPLPVITLRSEPQY
  DHTAGSSQSLSFPKLLHLSEHCVLTWTERGIYIFIPQNVQVLLWSEVKDIQDVAVCRNELFCLHLNGKVS
  HLSLISVERCVERLLRRGLWNLAARTCCLFQNSVIASRARKTLTADKLEHLKSQLDHGTYNDLISQLEEL
  ILKFEPLDSACSSRRSSISSHESFSILDSGIYRIISSRRGSQSDEDSCSLHSQTLSEDERFKEFTSQQEE
  DLPDQCCGSHGNEDNVSHAPVMFETDKNETFLPFGIPLPFRSPSPLVSLQAVKESVSSFVRKTTEKIGTL
  HTSPDLKVRPELRGDEQSCEEDVSSDTCPKEEDTEEEKEVTSPPPEEDRFQELKVATAEAMTKLQDPLVL
  FESESLRMVLQEWLSHLEKTFAMKDFSGVSDTDNSSMKLNQDVLLVNESKKGILDEDNEKEKRDSLGNEE
  SVDKTACECVRSPRESLDDLFQICSPCAIASGLRNDLAELTTLCLELNVLNSKIKSTSGHVDHTLQQYSP
  EILACQFLKKYFFLLNLKRAKESIKLSYSNSPSVWDTFIEGLKEMASSNPVYMEMEKGDLPTRLKLLDDE
  VPFDSPLLVVYATRLYEKFGESALRSLIKFFPSILPSDIIQLCHHHPAEFLAYLDSLVKSRPEDQRSSFL
  ESLLQPESLRLDWLLLAVSLDAPPSTSTMDDEGYPRPHSHLLSWGYSQLILHLIKLPADFITKEKMTDIC
  RSCGFWPGYLILCLELERRREAFTNIVYLNDMSLMEGDNGWIPETVEEWKLLLHLIQSKSTRPAPQESLN
  GSLSDGPSPINVENVALLLAKAMGPDRAWSLLQECGLALELSEKFTRTCDILRIAEKRQRALIQSMLEKC
  DRFLWSQQA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181507
• ORF Size: 3387 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181507.2
• RefSeq Size: 4880 bp
• RefSeq ORF: 3390 bp
• Locus ID: 11234
• UniProt ID: Q9UPZ3
• Cytogenetics: 11p15.1
• MW: 127.3 kDa
• Gene Summary: This gene encodes a protein that may play a role in organelle biogenesis associated with melanosomes, platelet dense granules, and lysosomes. This protein interacts with Hermansky-Pudlak syndrome 6 protein and may interact with the cytoplasmic domain of integrin, alpha-3. Mutations in this gene are associated with Hermansky-Pudlak syndrome type 5. Multiple transcript variants encoding two distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]