MYPOP (NM_001012643) Human Tagged ORF Clone

CAT#: RC218450

MYPOP (Myc-DDK-tagged)-Human Myb-related transcription factor, partner of profilin (MYPOP)

Product Images

Circular map for RC218450

• 

  Western blot validation of overexpression lysate (Cat# LY422891) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218450 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MYPOP
• Synonyms: P42pop
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218450 representing NM_001012643
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCGGCGGCGGCGGGCGAAGCGGAGGAAACCACCCGGTTGCGCAAGCCGCGCTTCTCATTCGAAG
  AGAACCAGATCCTGATCCGCGAGGTGCGCGCCCACTACCCGCAGCTCTACGGCGCGCAGAGCCGTCGGGT
  GAGCGTGGCAGAGCGGCGGCGCGTGTGGGACGGCATCGCCGCCAAGATCAACGGTATCACCAGCTGGAAG
  CGCACGGGCCAGGAGGTGCAGAAGCGCTGGAACGACTTCAAGCGCCGCACCAAGGAGAAGCTCGCTCGCG
  TGCCGCACTCCACGCAGGGCGCCGGGCCCGCCGCGGAGGACGCTTTCTCCGCGGAAGAGGAGACCATTTT
  TGCCATCCTGGGGCCAGGTGTGGCGGCGCCGGGGGCAGGTGCTGGGGCGGAGGAGCCCCCTGCGGCCCCC
  TCTTCACAGCCGCCGCCCCCAAGCGCCTGCCCCCAGCGCTACGTGTTGTCGGAAGACCGCCGGGAGGACC
  GACGTGCAGATACATCAGCCCACAGCAAGGCGGGCTCCAGCAGCCCGGAGCCATGGGCCCGGCCCTCCTG
  CACTCCCCAGGAAGGGGGCTGCCCACGGCCCAAGGAGCGTGAGTCACCACCCCCTTCGGCCCTGCAGCCG
  GTCCAGCTGCCTCGCCTGGCCTTGTCTCCACCACCCCCAGCCCCTCCACTGCCACCCCCACCGCCACTGG
  CCCAAGTGGCACCCTCACCCCCTAGCCCCCCACCCCCTCCTCGGCCTCCACCCACGCTCTCGGCCTCAGA
  CCCCTCCCTGGACTTCCTGCGGGCCCAGCAGGAGACTGCCAACGCCATCCGGGAGCTGGCCGGCACCCTT
  CGACAGGGACTGGCCAAACTGAGCGAGGCCCTCAGCGCTCTGCTGCCCCTTCTACCAGGAACCCCAGTTG
  ACTCCCTGCCTCCACCTCTGCCCCCACCCCCACCCCCACCGCCACCTCCCAGGCCTGTCCTGCCCCCACC
  GGCCCCCAAGGTGGAGATCACCCCAGAGCCCGTGTCCGTGGTGGCTGCTGTGGTGGACGGGGCAGTGGTG
  GCAGCCAGGGGAGTGATCATTGCCCCAAGGAGCGAGGAGGGGGCACCCCGGCCCCCCCCAGCCCCGCTCC
  CTCCGCACGACTCCCCCCCACACAAGCGGAGAAAAGGTTTCCCTACACGGAAAAGGCGCGGCCGATGGAA
  ATCTCCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218450 representing NM_001012643
  Red=Cloning site Green=Tags(s)
  
  MASAAAGEAEETTRLRKPRFSFEENQILIREVRAHYPQLYGAQSRRVSVAERRRVWDGIAAKINGITSWK
  RTGQEVQKRWNDFKRRTKEKLARVPHSTQGAGPAAEDAFSAEEETIFAILGPGVAAPGAGAGAEEPPAAP
  SSQPPPPSACPQRYVLSEDRREDRRADTSAHSKAGSSSPEPWARPSCTPQEGGCPRPKERESPPPSALQP
  VQLPRLALSPPPPAPPLPPPPPLAQVAPSPPSPPPPPRPPPTLSASDPSLDFLRAQQETANAIRELAGTL
  RQGLAKLSEALSALLPLLPGTPVDSLPPPLPPPPPPPPPPRPVLPPPAPKVEITPEPVSVVAAVVDGAVV
  AARGVIIAPRSEEGAPRPPPAPLPPHDSPPHKRRKGFPTRKRRGRWKSP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012643
• ORF Size: 1197 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012643.2, NP_001012661.1
• RefSeq Size: 1909 bp
• RefSeq ORF: 1200 bp
• Locus ID: 339344
• UniProt ID: Q86VE0
• Cytogenetics: 19q13.32
• MW: 42.3 kDa
• Gene Summary: Transcriptional repressor; DNA-binding protein that specifically recognizes the core sequence 5'-YAAC[GT]G-3'. Dimerization with PFN1 reduces its DNA-binding capacity (By similarity).[UniProtKB/Swiss-Prot Function]