KLHL3 (NM_017415) Human Tagged ORF Clone
CAT#: RC218393
KLHL3 (Myc-DDK-tagged)-Human kelch-like 3 (Drosophila) (KLHL3)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_017415" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | KLHL3 |
Synonyms | PHA2D |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC218393 representing NM_017415
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGTGAAAGTGTCAAGCTGAGCTCCCAGACTCTGATACAGGCTGGGGATGATGAGAAGAACCAGA GGACGATCACTGTCAACCCTGCCCACATGGGGAAAGCATTCAAGGTTATGAATGAACTGCGGAGTAAACA GCTGTTGTGTGACGTGATGATTGTGGCAGAAGATGTCGAGATAGAAGCCCACCGTGTGGTCCTGGCAGCC TGCAGCCCCTACTTCTGTGCGATGTTCACAGGTGACATGTCTGAGAGTAAAGCCAAAAAGATAGAAATCA AGGACGTGGATGGGCAGACGCTGAGTAAGCTGATTGACTACATCTATACTGCTGAAATCGAGGTGACTGA AGAGAATGTCCAGGTGCTGCTCCCGGCAGCCAGCTTGCTGCAGCTCATGGATGTTCGGCAGAACTGCTGT GACTTCCTGCAGTCTCAGTTGCATCCCACCAATTGCCTGGGCATCCGTGCATTTGCAGATGTACACACCT GCACTGACCTTCTGCAGCAGGCCAATGCCTACGCAGAGCAGCACTTTCCAGAGGTGATGCTAGGAGAAGA ATTTCTTAGCCTGAGTCTGGACCAGGTGTGCAGCTTGATATCCAGCGACAAGCTGACCGTTTCTTCAGAA GAGAAGGTGTTTGAAGCTGTGATCTCATGGATCAATTATGAGAAAGAAACCCGTTTAGAGCACATGGCAA AGCTGATGGAACATGTCCGACTTCCTCTCTTACCTAGGGACTACCTAGTCCAAACGGTTGAAGAAGAAGC TTTGATAAAGAATAACAACACCTGTAAAGACTTCCTCATTGAGGCCATGAAATACCATCTCCTCCCTCTG GATCAGAGACTATTGATTAAGAACCCAAGGACCAAGCCCAGGACTCCAGTCAGCCTTCCCAAGGTCATGA TTGTGGTTGGCGGCCAGGCACCCAAGGCAATCCGCAGTGTGGAGTGCTATGATTTCGAGGAGGACCGGTG GGATCAGATTGCTGAGCTTCCTTCCAGAAGATGCAGAGCAGGTGTGGTGTTCATGGCTGGCCACGTGTAT GCCGTGGGAGGGTTTAATGGCTCACTGCGGGTGCGGACAGTGGATGTGTATGACGGCGTGAAGGACCAGT GGACGTCCATTGCCAGCATGCAGGAGCGCCGGAGCACACTGGGCGCAGCGGTGCTCAATGACTTGCTCTA CGCAGTGGGAGGCTTTGATGGCAGTACTGGCCTAGCATCGGTGGAAGCCTACAGCTACAAGACCAACGAG TGGTTCTTTGTGGCCCCGATGAACACGCGGCGGAGCAGTGTGGGTGTGGGCGTTGTGGAGGGGAAGCTAT ATGCTGTTGGGGGTTATGATGGAGCTTCCCGCCAGTGTCTGAGCACTGTGGAGCAGTACAACCCAGCGAC CAATGAATGGATATACGTGGCGGACATGAGCACCCGCCGCAGTGGCGCAGGGGTTGGAGTGCTTAGCGGA CAGCTGTACGCCACAGGTGGGCATGATGGGCCTTTGGTGAGGAAGAGCGTTGAGGTTTACGATCCTGGAA CAAATACCTGGAAGCAAGTGGCAGACATGAACATGTGCCGGCGCAACGCAGGGGTCTGTGCAGTAAATGG GCTCCTGTATGTGGTTGGAGGGGATGATGGATCCTGCAACTTGGCTTCGGTGGAGTACTACAATCCTGTC ACTGACAAATGGACGCTGCTTCCAACGAACATGAGCACGGGGCGGAGCTATGCAGGTGTTGCCGTGATTC ACAAGTCCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218393 representing NM_017415
Red=Cloning site Green=Tags(s) MEGESVKLSSQTLIQAGDDEKNQRTITVNPAHMGKAFKVMNELRSKQLLCDVMIVAEDVEIEAHRVVLAA CSPYFCAMFTGDMSESKAKKIEIKDVDGQTLSKLIDYIYTAEIEVTEENVQVLLPAASLLQLMDVRQNCC DFLQSQLHPTNCLGIRAFADVHTCTDLLQQANAYAEQHFPEVMLGEEFLSLSLDQVCSLISSDKLTVSSE EKVFEAVISWINYEKETRLEHMAKLMEHVRLPLLPRDYLVQTVEEEALIKNNNTCKDFLIEAMKYHLLPL DQRLLIKNPRTKPRTPVSLPKVMIVVGGQAPKAIRSVECYDFEEDRWDQIAELPSRRCRAGVVFMAGHVY AVGGFNGSLRVRTVDVYDGVKDQWTSIASMQERRSTLGAAVLNDLLYAVGGFDGSTGLASVEAYSYKTNE WFFVAPMNTRRSSVGVGVVEGKLYAVGGYDGASRQCLSTVEQYNPATNEWIYVADMSTRRSGAGVGVLSG QLYATGGHDGPLVRKSVEVYDPGTNTWKQVADMNMCRRNAGVCAVNGLLYVVGGDDGSCNLASVEYYNPV TDKWTLLPTNMSTGRSYAGVAVIHKSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_017415 |
ORF Size | 1761 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_017415.3 |
RefSeq Size | 6485 bp |
RefSeq ORF | 1764 bp |
Locus ID | 26249 |
UniProt ID | Q9UH77 |
Cytogenetics | 5q31.2 |
Domains | BTB, Kelch |
MW | 64.8 kDa |
Gene Summary | This gene is ubiquitously expressed and encodes a full-length protein which has an N-terminal BTB domain followed by a BACK domain and six kelch-like repeats in the C-terminus. These kelch-like repeats promote substrate ubiquitination of bound proteins via interaction of the BTB domain with the CUL3 (cullin 3) component of a cullin-RING E3 ubiquitin ligase (CRL) complex. Muatations in this gene cause pseudohypoaldosteronism type IID (PHA2D); a rare Mendelian syndrome featuring hypertension, hyperkalaemia and metabolic acidosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC218393L3 | Lenti ORF clone of Human kelch-like 3 (Drosophila) (KLHL3), Myc-DDK-tagged |
USD 846.00 |
|
RC218393L4 | Lenti ORF clone of Human kelch-like 3 (Drosophila) (KLHL3), mGFP tagged |
USD 846.00 |
|
RG218393 | KLHL3 (tGFP-tagged) - Human kelch-like 3 (Drosophila) (KLHL3) |
USD 746.00 |
|
SC114103 | KLHL3 (untagged)-Human kelch-like 3 (Drosophila) (KLHL3) |
USD 547.00 |
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