RBMY1D (NM_001006120) Human Tagged ORF Clone

CAT#: RC218382

RBMY1D (Myc-DDK-tagged)-Human RNA binding motif protein, Y-linked, family 1, member D (RBMY1D). Note: ORF is codon optimized

Product Images

Circular map for RC218382

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RBMY1D
• Synonyms: hRBMY; RBM1; RBM2; RBMY1A1; YRRM1; YRRM2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218382 ORF sequence, codon optimized.
  Due to the complexity of NM_001006120, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAGAGGCCGATCACCCAGGTAAACTTTTTATAGGTGGATTGAATCGCGAAACAAATGAGAAGATGC
  TCAAGGCCGTGTTCGGCAAACACGGTCCCATAAGTGAAGTGTTGCTGATTAAGGATAGAACCAGCAAGTC
  CCGCGGCTTCGCTTTTATTACCTTTGAGAATCCTGCAGATGCCAAGAACGCCGCTAAAGATATGAACGGT
  AAGTCCCTGCACGGGAAGGCCATTAAGGTGGAACAGGCAAAAAAACCTAGCTTCCAGAGCGGCGGACGCA
  GGCGACCCCCAGCCAGTTCTCGCAACAGATCACCCAGTGGTTCTCTTAGGTCAGCGCGGGGTAGTCGAGG
  AGGTACAAGGGGATGGTTGCCCTCTCAGGAGGGCCATCTGGACGACGGCGGTTACACCCCAGATCTGAAG
  ATGAGTTATTCCAGAGGCCTGATACCAGTGAAAAGAGGCCCTTCCTCTCGCTCAGGGGGACCACCCCCAA
  AGAAGTCCGCACCAAGCGCCGTCGCTCGCAGCAATTCCTGGATGGGGAGTCAAGGGCCAATGTCACAGCG
  CAGAGAGAATTACGGAGTGCCTCCTAGAAGAGCAACTATCTCCTCTTGGCGGAACGATAGAATGTCCACT
  CGGCATGATGGTTATGCCACCAATGACGGAAACCACCCCAGCTGCCAGGAAACAAGAGACTACGCACCAC
  CTTCAAGAGGATATGCCTACAGGGATAATGGTCACTCCAATAGAGATGAACACTCTTCCAGAGGCTACCG
  AAACCACCGGTCTTCCAGGGAGACTCGAGACTACGCCCCCCCAAGCAGGGGACACGCATACCGAGATTAT
  GGCCACAGTAGACGAGACGAAAGTTACTCTCGGGGCTACCGGAACAGACGCAGTTCTCGGGAAACTAGAG
  AATATGCTCCGCCTTCACGAGGTCACGGGTATCGCGACTACGGTCATTCTCGCCGACATGAGTCATATTC
  TAGGGGTTACAGAAATCATCCTTCATCCCGCGAGACAAGGGACTATGCCCCCCCCCATAGAGATTATGCC
  TATAGGGACTACGGCCATAGCAGCTGGGACGAGCACAGCTCACGAGGCTACTCTTACCACGACGGGTATG
  GGGAAGCACTGGGACGCGACCACTCAGAACATCTGAGCGGATCCAGTTATCGGGATGCACTTCAGAGATA
  TGGAACCTCCCACGGCGCGCCCCCCGCCCGCGGGCCGCGCATGAGCTATGGGGGCTCAACATGTCATGCC
  TATTCCAATACCAGGGATCGCTACGGCAGGAGTTGGGAGAGCTATAGCTCATGTGGAGACTTTCACTACT
  GCGACAGAGAACACGTGTGTCGGAAAGATCAGCGGAACCCCCCGTCCCTGGGGAGAGTGCTCCCCGATCC
  TAGGGAGGCATGCGGGAGTAGCAGCTACGTCGCCAGTATTGTTGACGGCGGGGAATCAAGGTCCGAAAAG
  GGGGACAGCTCAAGATAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218382 representing NM_001006120
  Red=Cloning site Green=Tags(s)
  
  MVEADHPGKLFIGGLNRETNEKMLKAVFGKHGPISEVLLIKDRTSKSRGFAFITFENPADAKNAAKDMNG
  KSLHGKAIKVEQAKKPSFQSGGRRRPPASSRNRSPSGSLRSARGSRGGTRGWLPSQEGHLDDGGYTPDLK
  MSYSRGLIPVKRGPSSRSGGPPPKKSAPSAVARSNSWMGSQGPMSQRRENYGVPPRRATISSWRNDRMST
  RHDGYATNDGNHPSCQETRDYAPPSRGYAYRDNGHSNRDEHSSRGYRNHRSSRETRDYAPPSRGHAYRDY
  GHSRRDESYSRGYRNRRSSRETREYAPPSRGHGYRDYGHSRRHESYSRGYRNHPSSRETRDYAPPHRDYA
  YRDYGHSSWDEHSSRGYSYHDGYGEALGRDHSEHLSGSSYRDALQRYGTSHGAPPARGPRMSYGGSTCHA
  YSNTRDRYGRSWESYSSCGDFHYCDREHVCRKDQRNPPSLGRVLPDPREACGSSSYVASIVDGGESRSEK
  GDSSRY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001006120
• ORF Size: 1488 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001006120.2, NM_001006120.3, NP_001006120.2
• RefSeq Size: 1921 bp
• RefSeq ORF: 1491 bp
• Locus ID: 378949
• UniProt ID: P0C7P1
• Cytogenetics: Yq11.223
• MW: 55.8 kDa
• Gene Summary: This gene encodes a protein containing an RNA-binding motif in the N-terminus and four SRGY (serine, arginine, glycine, tyrosine) boxes in the C-terminus. This protein likely functions as a splicing factor during spermatogenesis. Multiple closely related paralogs of this gene are found in a gene cluster in the AZFb azoospermia factor region of chromosome Y. Most of these related copies are thought to be pseudogenes, though several likely encode functional proteins. [provided by RefSeq, Mar 2016]