C1orf172 (KDF1) (NM_152365) Human Tagged ORF Clone

CAT#: RC218144

C1orf172 (Myc-DDK-tagged)-Human chromosome 1 open reading frame 172 (C1orf172)

Product Images

Circular map for RC218144

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  Western blot validation of overexpression lysate (Cat# LY407614) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218144 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KDF1 protein (Cat# TP318144). The protein was produced from HEK293T cells transfected with KDF1 cDNA clone (Cat# RC218144) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C1orf172
• Synonyms: C1orf172; ECTD12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218144 representing NM_152365
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCGCCCTGGACACCCCCGCCCAGCATCTGGGCCTCCACGCTTGGGACCGTGGGAGCGGCCAACAG
  AGCTATGTCTGGAGACATATGATAAACCACCTCAGCCCCCACCAAGCCGCCGCACCCGTAGACCAGACCC
  CAAGGACCCTGGCCACCATGGGCCAGAGAGCATTACCTTCATCTCTGGCTCTGCTGAGCCGGCCCTTGAG
  TCCCCCACCTGCTGCCTGCTCTGGCGACCCTGGGTGTGGGAGTGGTGCCGGGCTGCCTTCTGCTTCCGCC
  GCTGCCGGGATTGCCTCCAGCGCTGTGGAGCCTGTGTGCGGGGATGCAGCCCCTGCCTGTCTACTGAGGA
  CTCCACTGAGGGGACTGCTGAAGCCAACTGGGCCAAGGAGCACAATGGAGTGCCCCCCAGCCCTGATCGT
  GCACCCCCCAGCCGGCGGGATGGCCAGCGGCTCAAGTCAACCATGGGCAGCAGCTTCAGCTACCCCGATG
  TTAAGCTCAAAGGCATCCCTGTGTATCCCTACCCGAGGGCCACCTCCCCAGCCCCTGATGCGGACTCCTG
  CTGCAAGGAGCCACTGGCCGATCCCCCACCCATGCGACACAGCCTGCCCAGCACCTTTGCCAGTAGTCCT
  CGTGGCTCCGAGGAGTACTATTCTTTCCATGAGTCGGACCTGGACCTGCCGGAGATGGGCAGTGGCTCCA
  TGTCGAGCCGAGAAATTGATGTGCTCATCTTCAAGAAGCTGACAGAGCTGTTCAGCGTACACCAGATCGA
  TGAGCTGGCCAAGTGCACATCAGACACTGTGTTCCTGGAGAAGACCAGTAAGATCTCGGACCTTATCAGC
  AGCATCACGCAGGACTACCACCTGGATGAGCAGGATGCTGAGGGCCGCCTGGTACGCGGCATCATTCGCA
  TTAGTACCCGAAAGAGCCGTGCTCGCCCACAGACCTCGGAGGGTCGTTCAACTCGGGCTGCTGCCCCAAC
  CGCTGCTGCCCCTGACAGTGGCCATGAGACCATGGTGGGCTCAGGTCTCAGCCAGGATGAGCTGACAGTG
  CAGATCTCCCAGGAGACGACTGCAGATGCCATCGCCCGGAAGCTGAGGCCTTATGGAGCTCCAGGGTACC
  CAGCAAGCCATGACTCATCCTTCCAGGGCACCGACACAGACTCGTCGGGGGCACCCTTGCTCCAGGTGTA
  CTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218144 representing NM_152365
  Red=Cloning site Green=Tags(s)
  
  MPRPGHPRPASGPPRLGPWERPTELCLETYDKPPQPPPSRRTRRPDPKDPGHHGPESITFISGSAEPALE
  SPTCCLLWRPWVWEWCRAAFCFRRCRDCLQRCGACVRGCSPCLSTEDSTEGTAEANWAKEHNGVPPSPDR
  APPSRRDGQRLKSTMGSSFSYPDVKLKGIPVYPYPRATSPAPDADSCCKEPLADPPPMRHSLPSTFASSP
  RGSEEYYSFHESDLDLPEMGSGSMSSREIDVLIFKKLTELFSVHQIDELAKCTSDTVFLEKTSKISDLIS
  SITQDYHLDEQDAEGRLVRGIIRISTRKSRARPQTSEGRSTRAAAPTAAAPDSGHETMVGSGLSQDELTV
  QISQETTADAIARKLRPYGAPGYPASHDSSFQGTDTDSSGAPLLQVYC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152365
• ORF Size: 1194 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152365.3
• RefSeq Size: 1785 bp
• RefSeq ORF: 1197 bp
• Locus ID: 126695
• UniProt ID: Q8NAX2
• Cytogenetics: 1p36.11
• MW: 43.5 kDa
• Gene Summary: Plays a role in the regulation of the epidermis formation during early development. Required both as an inhibitor of basal cell proliferation and a promoter of differentiation of basal progenitor cell progeny (By similarity).[UniProtKB/Swiss-Prot Function]