GDF 9 (GDF9) (NM_005260) Human Tagged ORF Clone
CAT#: RC217711
GDF9 (Myc-DDK-tagged)-Human growth differentiation factor 9 (GDF9)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_005260" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | GDF 9 |
Synonyms | POF14 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC217711 representing NM_005260.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCACGTCCCAACAAATTCCTCCTTTGGTTTTGCTGCTTTGCCTGGCTGTGTTTTCCTATTAGCCTT GGTTCTCAGGCTTCTGGGGGAGAAGCTCAGATTGCTGCTAGTGCTGAGTTGGAATCTGGGGCTATGCCT TGGTCCTTGCTGCAGCATATAGATGAGAGAGACAGAGCTGGCCTCCTTCCCGCGCTTTTCAAAGTTCTA TCTGTTGGGCGAGGTGGGTCACCTAGGCTGCAGCCAGACTCCAGAGCTTTGCACTACATGAAGAAGCTC TATAAGACATATGCTACCAAGGAAGGGATTCCTAAATCCAATAGAAGTCACCTCTACAACACTGTTCGG CTCTTCACCCCCTGTACCCGGCACAAGCAGGCTCCTGGAGACCAGGTAACAGGAATCCTTCCATCAGTG GAACTGCTATTTAACCTGGATCGCATTACTACTGTTGAACACTTACTCAAGTCAGTCTTGCTGTACAAT ATCAACAACTCAGTTTCTTTTTCCTCTGCTGTCAAATGTGTGTGCAATCTAATGATAAAGGAGCCAAAG TCTTCTAGCAGGACTCTCGGCAGAGCTCCATACTCATTTACCTTTAACTCACAGTTTGAATTTGGAAAG AAACACAAATGGATTCAGATTGATGTGACCAGCCTCCTTCAACCTTTAGTGGCCTCCAACAAGAGAAGT ATTCACATGTCTATAAATTTTACTTGCATGAAAGACCAGCTGGAGCATCCTTCAGCACAGAATGGTTTG TTTAACATGACTCTGGTGTCCCCCTCACTGATCTTATATTTGAATGACACAAGTGCTCAGGCTTATCAC AGCTGGTATTCCCTTCACTATAAAAGGAGGCCTTCCCAGGGTCCTGACCAGGAGAGAAGTCTGTCTGCC TATCCTGTGGGAGAAGAGGCTGCTGAGGATGGGAGATCTTCCCATCACCGTCACCGCAGAGGTCAGGAA ACTGTCAGTTCTGAATTGAAGAAGCCCTTGGGCCCAGCTTCCTTCAATCTGAGTGAATACTTCAGACAA TTTCTTCTTCCCCAAAATGAGTGTGAGCTCCATGACTTTAGACTTAGCTTTAGTCAGCTGAAGTGGGAC AACTGGATTGTGGCTCCGCACAGGTACAACCCTCGATACTGTAAAGGGGACTGTCCAAGGGCAGTTGGA CATCGGTATGGCTCTCCAGTTCACACCATGGTACAGAACATCATCTATGAGAAGCTGGACTCCTCAGTG CCAAGACCGTCATGTGTACCTGCCAAATACAGCCCCTTGAGTGTTTTGACCATTGAGCCCGATGGCTCA ATTGCCTATAAAGAGTACGAAGATATGATAGCTACAAAGTGCACCTGTCGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC217711
Blue=ORF Red=Cloning site Green=Tag(s) MARPNKFLLWFCCFAWLCFPISLGSQASGGEAQIAASAELESGAMPWSLLQHIDERDRAGLLPALFKVL SVGRGGSPRLQPDSRALHYMKKLYKTYATKEGIPKSNRSHLYNTVRLFTPCTRHKQAPGDQVTGILPSV ELLFNLDRITTVEHLLKSVLLYNINNSVSFSSAVKCVCNLMIKEPKSSSRTLGRAPYSFTFNSQFEFGK KHKWIQIDVTSLLQPLVASNKRSIHMSINFTCMKDQLEHPSAQNGLFNMTLVSPSLILYLNDTSAQAYH SWYSLHYKRRPSQGPDQERSLSAYPVGEEAAEDGRSSHHRHRRGQETVSSELKKPLGPASFNLSEYFRQ FLLPQNECELHDFRLSFSQLKWDNWIVAPHRYNPRYCKGDCPRAVGHRYGSPVHTMVQNIIYEKLDSSV PRPSCVPAKYSPLSVLTIEPDGSIAYKEYEDMIATKCTCR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_005260 |
ORF Size | 1362 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq Size | 1779 bp |
RefSeq ORF | 1365 bp |
Locus ID | 2661 |
UniProt ID | O60383 |
Cytogenetics | 5q31.1 |
Protein Families | Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Secreted Protein, Stem cell - Pluripotency, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transmembrane |
MW | 51.4 kDa |
Gene Summary | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates ovarian function. Reduced expression of this gene may be associated with polycystic ovary syndrome and mutations in this gene may be more common in mothers of dizygotic twins. [provided by RefSeq, Jul 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC217711L3 | Lenti ORF clone of Human growth differentiation factor 9 (GDF9), Myc-DDK-tagged |
USD 757.00 |
|
RC217711L4 | Lenti ORF clone of Human growth differentiation factor 9 (GDF9), mGFP tagged |
USD 757.00 |
|
RG217711 | GDF9 (tGFP-tagged) - Human growth differentiation factor 9 (GDF9) |
USD 657.00 |
|
SC303612 | GDF9 (untagged)-Human growth differentiation factor 9 (GDF9) |
USD 503.00 |
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