ASC 2 (NCOA6) (NM_014071) Human Tagged ORF Clone

CAT#: RC217698

NCOA6 (Myc-DDK-tagged)-Human nuclear receptor coactivator 6 (NCOA6)

Product Images

Circular map for RC217698

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ASC 2
• Synonyms: AIB3; ASC2; NRC; PRIP; RAP250; TRBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217698 representing NM_014071
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTTGGATGACCTTCCAAACTTAGAAGACATCTATACTTCCTTGTGTTCATCAACAATGGAAGACT
  CAGAGATGGATTTTGACTCTGGACTAGAAGATGATGACACAAAAAGTGATAGTATTTTGGAGGATTCCAC
  AATTTTTGTGGCCTTCAAAGGAAATATAGATGATAAAGACTTCAAATGGAAATTAGATGCAATATTGAAA
  AACGTGCCCAATTTGTTACACATGGAGTCCAGCAAGCTAAAAGTACAGAAGGTGGAGCCCTGGAACAGCG
  TGCGTGTGACATTCAACATCCCCCGGGAAGCAGCGGAGCGGCTACGGATCCTTGCTCAGAGCAACAACCA
  GCAGCTTCGGGATTTAGGGATTCTCTCCGTTCAGATTGAAGGGGAAGGTGCTATTAACCTGGCTTTGGCT
  CAGAACCGAAGCCAAGATGTGAGAATGAATGGACCCATGGGAGCTGGAAATTCAGTTAGGATGGAGGCGG
  GATTTCCTATGGCAAGTGGTCCAGGAATAATAAGGATGAACAACCCTGCCACTGTTATGATACCCCCGGG
  TGGAAATGTGTCATCTTCCATGATGGCACCAGGCCCCAATCCAGAGCTGCAGCCCAGGACCCCTCGCCCT
  GCTTCTCAGTCAGATGCAATGGATCCACTCCTCTCTGGGCTCCATATACAGCAGCAAAGTCATCCCTCAG
  GATCTTTAGCTCCCCCACATCACCCAATGCAGCCTGTCTCTGTGAACAGACAAATGAACCCAGCTAATTT
  TCCCCAGCTGCAGCAGCAGCAGCAACAACAACAACAGCAGCAGCAGCAGCAGCAGCAGCAACAACAGCAA
  CAGCAGCAACAACAGTTGCAGGCAAGACCCCCACAGCAACATCAGCAGCAACAGCCACAGGGAATTCGAC
  CCCAGTTTACTGCCCCAACTCAGGTGCCTGTTCCTCCAGGCTGGAACCAGCTGCCTTCTGGAGCCCTTCA
  ACCTCCTCCAGCCCAGGGTTCTCTGGGCACAATGACTGCAAACCAAGGGTGGAAGAAGGCTCCCTTGCCC
  GGCCCAATGCAACAGCAACTCCAGGCAAGACCATCCTTAGCCACGGTACAGACGCCTTCCCACCCTCCCC
  CTCCATATCCCTTTGGCAGCCAGCAAGCCTCACAAGCCCACACAAACTTTCCTCAGATGAGCAACCCAGG
  CCAGTTCACAGCTCCTCAGATGAAGAGTTTGCAGGGAGGGCCCTCTAGGGTCCCAACTCCCTTGCAGCAG
  CCCCACCTCACCAACAAGTCTCCTGCCTCCTCACCCTCCTCCTTCCAGCAGGGATCCCCTGCATCCTCCC
  CAACGGTTAACCAAACTCAGCAGCAGATGGGACCAAGGCCACCTCAAAATAACCCACTTCCCCAGGGATT
  TCAGCAGCCTGTCAGCTCTCCGGGTCGGAATCCTATGGTTCAACAGGGAAATGTGCCACCTAACTTCATG
  GTGATGCAGCAGCAACCACCAAACCAGGGGCCACAGAGTTTACATCCAGGCCTAGGAGGAATGCCTAAAC
  GCCTCCCACCTGGCTTCTCAGCAGGACAGGCCAATCCGAACTTTATGCAAGGTCAGGTGCCTTCGACCAC
  AGCAACCACCCCTGGGAATTCAGGAGCCCCTCAGCTGCAAGCAAATCAAAATGTCCAGCATGCAGGTGGT
  CAAGGAGCTGGTCCTCCTCAAAACCAGATGCAGGTGTCCCACGGGCCGCCAAATATGATGCAGCCCAGCC
  TCATGGGAATTCATGGCAACATGAACAATCAGCAGGCTGGTACTTCTGGGGTTCCTCAAGTGAACCTCAG
  CAACATGCAAGGCCAGCCCCAGCAGGGCCCACCATCTCAGCTGATGGGCATGCACCAGCAAATCGTGCCC
  TCCCAGGGCCAGATGGTCCAGCAACAAGGAACCTTGAACCCTCAGAACCCTATGATCCTTTCAAGGGCCC
  AGCTTATGCCACAGGGCCAGATGATGGTGAACCCCCCGAGCCAAAATCTTGGGCCCTCGCCCCAAAGGAT
  GACCCCACCCAAGCAGATGCTTTCCCAGCAGGGCCCACAAATGATGGCGCCACATAACCAGATGATGGGG
  CCTCAGGGGCAGGTTTTGCTCCAACAGAACCCAATGATAGAGCAGATTATGACCAATCAAATGCAGGGGA
  ATAAGCAGCAGTTTAACACTCAGAACCAGTCCAATGTCATGCCGGGACCAGCCCAGATAATGAGGGGACC
  AACTCCGAACATGCAAGGAAATATGGTGCAGTTTACGGGACAGATGTCAGGACAGATGCTGCCCCAGCAA
  GGGCCTGTGAACAACAGTCCATCTCAGGTTATGGGCATTCAGGGACAGGTCCTGCGGCCACCAGGGCCCA
  GCCCACACATGGCCCAGCAGCATGGTGATCCTGCTACTACAGCAAATAACGATGTCAGTTTATCTCAGAT
  GATGCCTGATGTTAGCATTCAACAAACCAACATGGTCCCCCCTCATGTGCAGGCCATGCAGGGAAACAGT
  GCCTCGGGAAACCACTTCTCAGGCCATGGGATGTCTTTCAATGCACCTTTCAGTGGAGCTCCCAATGGAA
  ATCAGATGTCCTGTGGTCAAAATCCAGGCTTCCCAGTCAATAAGGATGTCACGCTAACGAGCCCATTGTT
  GGTCAACTTATTGCAGAGTGACATATCTGCAGGCCATTTTGGGGTAAACAATAAGCAAAATAATACCAAC
  GCAAATAAACCGAAGAAGAAGAAACCCCCTCGGAAGAAGAAAAATAGTCAGCAAGATCTAAACACCCCAG
  ATACTCGCCCAGCTGGTCTGGAAGAGGCTGATCAGCCACCGTTGCCTGGAGAACAAGGAATTAACTTGGA
  TAACTCAGGCCCTAAACTGCCAGAATTTTCAAACCGGCCACCAGGTTATCCTTCTCAACCAGTTGAACAG
  AGGCCACTTCAGCAGATGCCTCCTCAACTCATGCAGCATGTGGCACCCCCACCACAGCCACCACAGCAGC
  AGCCACAGCCACAACTGCCTCAGCAGCAGCAGCCACCACCTCCCAGTCAGCCACAGTCTCAGCAGCAGCA
  GCAGCAGCAGCAACAAATGATGATGATGCTCATGATGCAGCAGGATCCCAAATCAGTTAGGCTTCCAGTC
  TCTCAAAATGTCCATCCTCCAAGGGGCCCCCTGAACCCCGACTCCCAGAGAATGCCCATGCAACAGAGTG
  GCAGTGTGCCTGTCATGGTCAGTCTGCAAGGACCTGCCTCCGTGCCACCATCACCTGATAAACAAAGAAT
  GCCAATGCCTGTGAATACTCCCTTGGGAAGCAATTCAAGGAAAATGGTCTATCAGGAGAGCCCGCAGAAT
  CCTTCCAGCTCGCCACTGGCGGAGATGGCCTCACTCCCTGAAGCAAGTGGCAGTGAAGCACCATCTGTCC
  CAGGAGGCCCAAACAACATGCCTTCACATGTAGTACTTCCCCAGAATCAGTTAATGATGACAGGGCCAAA
  ACCTGGACCATCGCCCCTTTCAGCAACTCAAGGTGCAACTCCCCAGCAACCCCCTGTAAATTCCCTGCCC
  AGCTCTCACGGCCACCACTTCCCAAATGTGGCTGCGCCAACCCAGACATCTAGGCCCAAAACACCAAACA
  GAGCCAGCCCCAGACCCTATTATCCTCAGACACCCAACAACCGCCCTCCCAGCACAGAACCTTCAGAAAT
  CAGTCTGTCACCAGAAAGACTCAATGCCTCCATAGCAGGACTCTTCCCTCCACAGATTAATATTCCTTTA
  CCTCCTAGGCCAAATTTAAACAGGGGCTTTGATCAACAAGGCCTAAATCCAACAACTTTGAAGGCCATCG
  GGCAAGCACCTTCAAATCTTACCATGAATCCTTCCAATTTTGCTACCCCACAAACTCACAAATTAGATTC
  TGTGGTAGTGAATTCTGGAAAGCAGTCTAATTCTGGAGCAACAAAACGGGCAAGTCCAAGCAACAGTCGC
  AGGTCTAGTCCTGGGTCCAGTAGGAAAACCACTCCAAGCCCTGGGAGGCAAAATTCAAAAGCCCCTAAAC
  TTACTCTGGCCTCTCAGACAAATGCAGCCCTATTGCAGAATGTGGAGTTGCCGAGAAATGTATTGGTCAG
  TCCCACTCCTCTGGCCAATCCCCCTGTACCTGGGAGCTTTCCTAACAACAGTGGGCTGAATCCTCAGAAT
  TCTACTGTGTCTGTGGCTGCAGTTGGGGGTGTTGTTGAGGATAACAAGGAGAGCTTGAATGTGCCTCAGG
  ACAGTGATTGCCAGAATTCCCAGAGTAGGAAGGAACAGGTAAACATTGAACTAAAAGCAGTCCCTGCCCA
  AGAAGTTAAAATGGTTGTCCCTGAAGATCAGTCCAAAAAGGATGGGCAGCCTTCGGATCCTAACAAACTT
  CCCAGTGTCGAAGAGAACAAAAATTTGGTGTCTCCTGCTATGAGGGAAGCACCAACATCGTTAAGTCAAC
  TTCTTGACAACTCTGGAGCTCCCAATGTGACAATTAAACCCCCTGGGCTTACAGATCTGGAAGTAACACC
  TCCAGTAGTTTCTGGGGAGGACCTCAAAAAAGCATCTGTCATTCCCACACTGCAGGATCTGTCTTCTTCT
  AAAGAACCTTCTAATTCCCTAAACTTACCTCACAGTAATGAGCTGTGTTCATCCCTTGTGCATCCCGAAT
  TGAGTGAGGTCAGTTCTAACGTTGCACCAAGCATCCCTCCAGTAATGTCAAGACCTGTTAGCTCTTCCTC
  CATTTCCACTCCCTTGCCCCCAAATCAAATAACTGTATTTGTCACTTCCAATCCCATCACAACTTCAGCT
  AACACATCAGCAGCTTTGCCAACTCACTTGCAGTCTGCATTGATGTCAACAGTTGTCACAATGCCCAATG
  CGGGTAGCAAGGTTATGGTTTCTGAGGGACAGTCAGCTGCTCAGTCTAATGCCCGGCCTCAGTTCATTAC
  ACCTGTCTTTATCAATTCATCCTCAATAATTCAGGTTATGAAAGGATCACAGCCAAGCACAATTCCTGCA
  GCCCCACTGACAACCAACTCTGGCCTGATGCCTCCCTCTGTTGCAGTTGTTGGCCCTTTACACATACCTC
  AGAACATAAAATTTTCTTCTGCTCCTGTACCGCCTAATGCCCTCTCCAGTAGTCCTGCTCCAAACATCCA
  GACAGGTCGACCTTTGGTCCTTAGCTCACGAGCCACCCCTGTTCAGCTTCCTTCCCCTCCTTGTACGTCT
  TCTCCAGTTGTCCCTTCTCATCCCCCTGTGCAGCAAGTGAAAGAATTGAATCCAGATGAGGCTAGCCCTC
  AGGTGAACACCTCAGCAGATCAGAACACTCTTCCCTCTTCACAGTCAACCACAATGGTTTCTCCCCTTTT
  GACCAATAGTCCAGGGTCCTCTGGCAACCGGCGAAGCCCAGTCTCGTCTAGTAAGGGCAAAGGAAAAGTG
  GACAAAATTGGCCAAATTTTGTTGACCAAGGCATGTAAGAAAGTTACAGGCTCTCTTGAGAAAGGGGAAG
  AACAATATGGTGCAGATGGAGAGACTGAAGGCCAAGGGCTAGACACCACAGCTCCGGGGCTCATGGGAAC
  AGAGCAGTTATCCACAGAGCTGGACAGTAAAACCCCAACGCCCCCAGCACCCACTCTGCTAAAAATGACC
  TCTAGCCCTGTGGGCCCGGGCACTGCCTCAGCAGGACCCAGCTTACCTGGCGGTGCTCTCCCCACCAGTG
  TACGCTCGATAGTAACCACTCTGGTACCCTCCGAGCTCATCTCCGCCGTACCGACCACAAAAAGCAATCA
  TGGTGGCATAGCATCTGAGTCACTTGCGGGTGGCCTAGTGGAGGAGAAGGTGGGATCCCATCCAGAACTT
  CTACCCAGCATAGCCCCGTCGCAGAATTTAGTCTCAAAGGAAACTTCAACCACAGCACTGCAGGCCTCTG
  TTGCCAGACCAGAGCTGGAGGTAAATGCTGCCATAGTCTCTGGACAAAGCAGTGAGCCCAAAGAGATAGT
  TGAAAAGTCCAAAATCCCAGGCCGAAGAAACTCCCGAACTGAAGAGCCAACTGTGGCCTCTGAAAGTGTG
  GAAAATGGACATCGTAAACGATCTTCTCGACCTGCTTCAGCCTCCAGCTCTACTAAAGACATAACCAGTG
  CGGTGCAATCCAAGCGAAGAAAATCCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217698 representing NM_014071
  Red=Cloning site Green=Tags(s)
  
  MVLDDLPNLEDIYTSLCSSTMEDSEMDFDSGLEDDDTKSDSILEDSTIFVAFKGNIDDKDFKWKLDAILK
  NVPNLLHMESSKLKVQKVEPWNSVRVTFNIPREAAERLRILAQSNNQQLRDLGILSVQIEGEGAINLALA
  QNRSQDVRMNGPMGAGNSVRMEAGFPMASGPGIIRMNNPATVMIPPGGNVSSSMMAPGPNPELQPRTPRP
  ASQSDAMDPLLSGLHIQQQSHPSGSLAPPHHPMQPVSVNRQMNPANFPQLQQQQQQQQQQQQQQQQQQQQ
  QQQQQLQARPPQQHQQQQPQGIRPQFTAPTQVPVPPGWNQLPSGALQPPPAQGSLGTMTANQGWKKAPLP
  GPMQQQLQARPSLATVQTPSHPPPPYPFGSQQASQAHTNFPQMSNPGQFTAPQMKSLQGGPSRVPTPLQQ
  PHLTNKSPASSPSSFQQGSPASSPTVNQTQQQMGPRPPQNNPLPQGFQQPVSSPGRNPMVQQGNVPPNFM
  VMQQQPPNQGPQSLHPGLGGMPKRLPPGFSAGQANPNFMQGQVPSTTATTPGNSGAPQLQANQNVQHAGG
  QGAGPPQNQMQVSHGPPNMMQPSLMGIHGNMNNQQAGTSGVPQVNLSNMQGQPQQGPPSQLMGMHQQIVP
  SQGQMVQQQGTLNPQNPMILSRAQLMPQGQMMVNPPSQNLGPSPQRMTPPKQMLSQQGPQMMAPHNQMMG
  PQGQVLLQQNPMIEQIMTNQMQGNKQQFNTQNQSNVMPGPAQIMRGPTPNMQGNMVQFTGQMSGQMLPQQ
  GPVNNSPSQVMGIQGQVLRPPGPSPHMAQQHGDPATTANNDVSLSQMMPDVSIQQTNMVPPHVQAMQGNS
  ASGNHFSGHGMSFNAPFSGAPNGNQMSCGQNPGFPVNKDVTLTSPLLVNLLQSDISAGHFGVNNKQNNTN
  ANKPKKKKPPRKKKNSQQDLNTPDTRPAGLEEADQPPLPGEQGINLDNSGPKLPEFSNRPPGYPSQPVEQ
  RPLQQMPPQLMQHVAPPPQPPQQQPQPQLPQQQQPPPPSQPQSQQQQQQQQQMMMMLMMQQDPKSVRLPV
  SQNVHPPRGPLNPDSQRMPMQQSGSVPVMVSLQGPASVPPSPDKQRMPMPVNTPLGSNSRKMVYQESPQN
  PSSSPLAEMASLPEASGSEAPSVPGGPNNMPSHVVLPQNQLMMTGPKPGPSPLSATQGATPQQPPVNSLP
  SSHGHHFPNVAAPTQTSRPKTPNRASPRPYYPQTPNNRPPSTEPSEISLSPERLNASIAGLFPPQINIPL
  PPRPNLNRGFDQQGLNPTTLKAIGQAPSNLTMNPSNFATPQTHKLDSVVVNSGKQSNSGATKRASPSNSR
  RSSPGSSRKTTPSPGRQNSKAPKLTLASQTNAALLQNVELPRNVLVSPTPLANPPVPGSFPNNSGLNPQN
  STVSVAAVGGVVEDNKESLNVPQDSDCQNSQSRKEQVNIELKAVPAQEVKMVVPEDQSKKDGQPSDPNKL
  PSVEENKNLVSPAMREAPTSLSQLLDNSGAPNVTIKPPGLTDLEVTPPVVSGEDLKKASVIPTLQDLSSS
  KEPSNSLNLPHSNELCSSLVHPELSEVSSNVAPSIPPVMSRPVSSSSISTPLPPNQITVFVTSNPITTSA
  NTSAALPTHLQSALMSTVVTMPNAGSKVMVSEGQSAAQSNARPQFITPVFINSSSIIQVMKGSQPSTIPA
  APLTTNSGLMPPSVAVVGPLHIPQNIKFSSAPVPPNALSSSPAPNIQTGRPLVLSSRATPVQLPSPPCTS
  SPVVPSHPPVQQVKELNPDEASPQVNTSADQNTLPSSQSTTMVSPLLTNSPGSSGNRRSPVSSSKGKGKV
  DKIGQILLTKACKKVTGSLEKGEEQYGADGETEGQGLDTTAPGLMGTEQLSTELDSKTPTPPAPTLLKMT
  SSPVGPGTASAGPSLPGGALPTSVRSIVTTLVPSELISAVPTTKSNHGGIASESLAGGLVEEKVGSHPEL
  LPSIAPSQNLVSKETSTTALQASVARPELEVNAAIVSGQSSEPKEIVEKSKIPGRRNSRTEEPTVASESV
  ENGHRKRSSRPASASSSTKDITSAVQSKRRKSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014071
• ORF Size: 6189 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014071.5
• RefSeq Size: 7071 bp
• RefSeq ORF: 6192 bp
• Locus ID: 23054
• UniProt ID: Q14686
• Cytogenetics: 20q11.22
• Protein Families: Druggable Genome, Transcription Factors
• MW: 219.1 kDa
• Gene Summary: The protein encoded by this gene is a transcriptional coactivator that can interact with nuclear hormone receptors to enhance their transcriptional activator functions. This protein has been shown to be involved in the hormone-dependent coactivation of several receptors, including prostanoid, retinoid, vitamin D3, thyroid hormone, and steroid receptors. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jun 2011]