REV1 (NM_001037872) Human Tagged ORF Clone

CAT#: RC217528

REV1 (Myc-DDK-tagged)-Human REV1 homolog (S. cerevisiae) (REV1), transcript variant 2

Product Images

Circular map for RC217528

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: REV1
• Synonyms: AIBP80; REV1L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217528 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCGAGGTGGATGGAGGAAGCGAGCTGAAAATGATGGCTGGGAAACATGGGGTGGGTATATGGCTG
  CCAAGGTCCAGAAATTGGAGGAACAGTTTCGATCAGATGCTGCTATGCAGAAGGATGGGACTTCATCTAC
  AATTTTTAGTGGAGTTGCCATCTATGTTAATGGATACACAGATCCTTCCGCTGAGGAATTGAGAAAACTA
  ATGATGTTGCATGGAGGTCAATACCATGTATATTATTCCAGATCTAAAACAACACATATTATTGCCACAA
  ATCTTCCCAATGCCAAAATTAAAGAATTAAAGGGGGAAAAAGTAATTCGACCAGAATGGATTGTGGAAAG
  CATCAAAGCTGGACGACTCCTCTCCTACATTCCATATCAGCTGTACACCAAGCAGTCCAGTATGCAGAAA
  GGTCTCAGCTTTAATCCTGTATGCAGACCTGAGGATCCTCTGCCAGGTCCAAGCAATATAGCCAAACAGC
  TCAACAACAGGGTAAATCACATCGTTAAGAAGATTGAAACGGAAAATGAAGTCAAAGTCAATGGCATGAA
  CAGTTGGAATGAAGAAGATGAAAATAATGATTTTAGTTTTGTGGATCTGGAGCAGACCTCTCCGGGAAGG
  AAACAGAATGGAATTCCGCATCCCAGAGGGAGCACTGCCATTTTTAATGGACACACTCCTAGCTCTAATG
  GTGCCTTAAAGACACAGGATTGCTTGGTGCCCATGGTCAACAGTGTTGCCAGCAGGCTTTCTCCAGCCTC
  TTCCCAGGAGGAGGATAAGGCTGAGAAGAGCAGCACTGATTTCAGAGACTGCACTCTGCAGCAGTTGCAG
  CAAAGCACCAGAAACACAGATGCTTTGCGGAATCCACACAGAACTAATTCTTTCTCATTATCACCTTTGC
  ACAGTAACACTAAAATCAATGGTGCTCACCACTCCACTGTTCAGGGGCCTTCAAGCACAAAAAGCACTTC
  TTCAGTATCTACGTTTAGCAAGGCAGCACCTTCAGTGCCATCCAAACCTTCAGACTGCAATTTTATTTCA
  AACTTCTATTCTCATTCAAGACTGCATCACATATCAATGTGGAAGTGTGAATTGACTGAGTTTGTCAATA
  CCCTACAAAGACAAAGTAATGGTATCTTTCCAGGAAGGGAAAAGTTAAAAAAAATGAAAACAGGCAGGTC
  TGCACTTGTTGTAACTGACACAGGAGATATGTCAGTATTGAATTCTCCCAGACATCAGAGCTGTATAATG
  CATGTTGATATGGATTGCTTCTTTGTATCAGTGGGTATACGAAATAGACCAGATCTCAAAGGAAAACCAG
  TGGCTGTTACAAGTAACAGAGGCACAGGAAGGGCACCTTTACGTCCTGGCGCTAACCCCCAGCTGGAGTG
  GCAGTATTACCAGAATAAAATCCTGAAAGGCAAAGCAGATATACCAGATTCATCATTGTGGGAGAATCCA
  GATTCTGCGCAAGCAAATGGAATTGATTCTGTTTTGTCAAGGGCTGAAATTGCATCTTGTAGTTATGAGG
  CCAGGCAACTTGGCATTAAGAACGGAATGTTTTTTGGGCATGCTAAACAACTATGTCCTAATCTTCAAGC
  TGTTCCATACGATTTTCATGCATATAAGGAAGTCGCACAAACATTGTATGAAACATTGGCAAGCTACACT
  CATAACATTGAAGCTGTCAGTTGTGATGAAGCGCTGGTAGACATTACCGAAATCCTTGCAGAGACCAAAC
  TTACTCCTGATGAATTTGCAAATGCTGTTCGTATGGAAATCAAAGACCAGACGAAATGTGCTGCCTCTGT
  TGGAATTGGTTCTAATATTCTCCTGGCTAGAATGGCAACTAGAAAAGCAAAACCAGATGGGCAGTACCAC
  CTAAAACCAGAAGAAGTAGATGATTTTATCAGAGGCCAGCTAGTGACCAATCTACCAGGAGTTGGACATT
  CAATGGAATCTAAGTTGGCATCTTTGGGAATTAAAACTTGTGGAGACTTGCAGTATATGACCATGGCAAA
  ACTCCAAAAAGAATTTGGTCCCAAAACAGGTCAGATGCTTTATAGGTTCTGCCGTGGCTTGGATGATAGA
  CCAGTTCGAACTGAAAAGGAAAGAAAATCTGTTTCAGCTGAGATCAACTATGGAATAAGGTTTACTCAGC
  CAAAAGAGGCAGAAGCTTTTCTTCTGAGTCTTTCAGAAGAAATTCAAAGAAGACTAGAAGCCACTGGCAT
  GAAGGGTAAACGTCTAACTCTCAAAATCATGGTACGAAAGCCTGGGGCTCCTGTAGAAACTGCAAAATTT
  GGAGGCCATGGAATTTGTGATAACATTGCCAGGACTGTAACTCTTGACCAGGCAACAGATAATGCAAAAA
  TAATTGGAAAGGCGATGCTAAACATGTTTCATACAATGAAACTAAATATATCAGATATGAGAGGGGTTGG
  GATTCACGTGAATCAGTTGGTTCCAACTAATCTGAACCCTTCCACATGTCCCAGTCGCCCATCAGTTCAG
  TCAAGCCACTTTCCTAGTGGGTCATACTCTGTCCGTGATGTCTTCCAAGTTCAGAAAGCTAAGAAATCCA
  CCGAAGAGGAGCACAAAGAAGTATTTCGGGCTGCTGTGGATCTGGAAATATCATCTGCTTCTAGAACTTG
  CACTTTCTTGCCACCTTTTCCTGCACATCTGCCGACCAGTCCTGATACTAACAAGGCTGAGTCTTCAGGG
  AAATGGAATGGTCTACATACTCCTGTCAGTGTGCAGTCGAGACTTAACCTGAGTATAGAGGTCCCGTCAC
  CTTCCCAGCTGGATCAGTCTGTTTTAGAAGCACTTCCACCTGATCTCCGGGAACAAGTAGAGCAAGTCTG
  TGCTGTCCAGCAAGCAGAGTCACATGGCGACAAAAAGAAAGAACCAGTAAATGGCTGTAATACAGGAATT
  TTGCCACAACCAGTTGGGACAGTCTTGTTGCAAATACCAGAACCTCAAGAATCGAACAGTGACGCAGGAA
  TAAATTTAATAGCCCTTCCAGCATTTTCACAGGTGGACCCTGAGGTATTTGCTGCCCTTCCTGCTGAACT
  TCAGAGGGAGCTGAAAGCAGCGTATGATCAAAGACAAAGGCAGGGCGAGAACAGCACTCACCAGCAGTCA
  GCCAGCGCATCTGTGCCAAAGAATCCTTTACTTCATCTAAAGGCAGCAGTGAAAGAAAAGAAAAGAAACA
  AGAAGAAAAAAACCATCGGTTCACCAAAAAGGATTCAGAGTCCTTTGAATAACAAGCTGCTTAACAGTCC
  TGCAAAAACTCTGCCAGGGGCCTGTGGCAGTCCCCAGAAGTTAATTGATGGGTTTCTAAAACATGAAGGA
  CCTCCTGCAGAGAAACCCCTGGAAGAACTCTCTGCTTCTACTTCAGGTGTGCCAGGCCTTTCTAGTTTGC
  AGTCTGACCCAGCTGGCTGTGTGAGACCTCCAGCACCCAATCTAGCTGGAGCTGTTGAATTCAATGATGT
  GAAGACCTTGCTCAGAGAATGGATAACTACAATTTCAGATCCAATGGAAGAAGACATTCTCCAAGTTGTG
  AAATACTGTACTGATCTAATAGAAGAAAAAGATTTGGAAAAACTGGATCTAGTTATAAAATACATGAAAA
  GGCTGATGCAGCAATCGGTGGAATCGGTTTGGAATATGGCATTTGACTTTATTCTTGACAATGTCCAGGT
  GGTTTTACAACAAACTTATGGAAGCACATTAAAAGTTACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217528 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRRGGWRKRAENDGWETWGGYMAAKVQKLEEQFRSDAAMQKDGTSSTIFSGVAIYVNGYTDPSAEELRKL
  MMLHGGQYHVYYSRSKTTHIIATNLPNAKIKELKGEKVIRPEWIVESIKAGRLLSYIPYQLYTKQSSMQK
  GLSFNPVCRPEDPLPGPSNIAKQLNNRVNHIVKKIETENEVKVNGMNSWNEEDENNDFSFVDLEQTSPGR
  KQNGIPHPRGSTAIFNGHTPSSNGALKTQDCLVPMVNSVASRLSPASSQEEDKAEKSSTDFRDCTLQQLQ
  QSTRNTDALRNPHRTNSFSLSPLHSNTKINGAHHSTVQGPSSTKSTSSVSTFSKAAPSVPSKPSDCNFIS
  NFYSHSRLHHISMWKCELTEFVNTLQRQSNGIFPGREKLKKMKTGRSALVVTDTGDMSVLNSPRHQSCIM
  HVDMDCFFVSVGIRNRPDLKGKPVAVTSNRGTGRAPLRPGANPQLEWQYYQNKILKGKADIPDSSLWENP
  DSAQANGIDSVLSRAEIASCSYEARQLGIKNGMFFGHAKQLCPNLQAVPYDFHAYKEVAQTLYETLASYT
  HNIEAVSCDEALVDITEILAETKLTPDEFANAVRMEIKDQTKCAASVGIGSNILLARMATRKAKPDGQYH
  LKPEEVDDFIRGQLVTNLPGVGHSMESKLASLGIKTCGDLQYMTMAKLQKEFGPKTGQMLYRFCRGLDDR
  PVRTEKERKSVSAEINYGIRFTQPKEAEAFLLSLSEEIQRRLEATGMKGKRLTLKIMVRKPGAPVETAKF
  GGHGICDNIARTVTLDQATDNAKIIGKAMLNMFHTMKLNISDMRGVGIHVNQLVPTNLNPSTCPSRPSVQ
  SSHFPSGSYSVRDVFQVQKAKKSTEEEHKEVFRAAVDLEISSASRTCTFLPPFPAHLPTSPDTNKAESSG
  KWNGLHTPVSVQSRLNLSIEVPSPSQLDQSVLEALPPDLREQVEQVCAVQQAESHGDKKKEPVNGCNTGI
  LPQPVGTVLLQIPEPQESNSDAGINLIALPAFSQVDPEVFAALPAELQRELKAAYDQRQRQGENSTHQQS
  ASASVPKNPLLHLKAAVKEKKRNKKKKTIGSPKRIQSPLNNKLLNSPAKTLPGACGSPQKLIDGFLKHEG
  PPAEKPLEELSASTSGVPGLSSLQSDPAGCVRPPAPNLAGAVEFNDVKTLLREWITTISDPMEEDILQVV
  KYCTDLIEEKDLEKLDLVIKYMKRLMQQSVESVWNMAFDFILDNVQVVLQQTYGSTLKVT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037872
• ORF Size: 3750 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037872.3
• RefSeq Size: 4748 bp
• RefSeq ORF: 3753 bp
• Locus ID: 51455
• UniProt ID: Q9UBZ9
• Cytogenetics: 2q11.2
• Protein Families: Druggable Genome
• MW: 138.1 kDa
• Gene Summary: This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. [provided by RefSeq, Mar 2016]