RUFY1 (NM_025158) Human Tagged Lenti ORF Clone
CAT#: RC217181L1
- LentiORF®
Lenti ORF clone of Human RUN and FYVE domain containing 1 (RUFY1), transcript variant 1, Myc-DDK-tagged
"NM_025158" in other vectors (7)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | RUFY1 |
Synonyms | RABIP4; ZFYVE12 |
Vector | pLenti-C-Myc-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC217181).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_025158 |
ORF Size | 2124 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025158.3 |
RefSeq Size | 2644 bp |
RefSeq ORF | 2127 bp |
Locus ID | 80230 |
UniProt ID | Q96T51 |
Cytogenetics | 5q35.3 |
Domains | FYVE, RUN |
Protein Pathways | Endocytosis |
MW | 79.6 kDa |
Gene Summary | This gene encodes a protein that contains a RUN domain and a FYVE-type zinc finger domain. The encoded protein binds to phosphatidylinositol-3-phosphate (PI3P) and plays a role in early endosomal trafficking, tethering and fusion through interactions with small GTPases including Rab4, Rab5 and Rab14. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC217181 | RUFY1 (Myc-DDK-tagged)-Human RUN and FYVE domain containing 1 (RUFY1), transcript variant 1 |
USD 648.00 |
|
RC217181L2 | Lenti ORF clone of Human RUN and FYVE domain containing 1 (RUFY1), transcript variant 1, mGFP tagged |
USD 948.00 |
|
RC217181L3 | Lenti ORF clone of Human RUN and FYVE domain containing 1 (RUFY1), transcript variant 1, Myc-DDK-tagged |
USD 948.00 |
|
RC217181L4 | Lenti ORF clone of Human RUN and FYVE domain containing 1 (RUFY1), transcript variant 1, mGFP tagged |
USD 948.00 |
|
RG217181 | RUFY1 (tGFP-tagged) - Human RUN and FYVE domain containing 1 (RUFY1), transcript variant 1 |
USD 848.00 |
|
SC110125 | RUFY1 (untagged)-Human RUN and FYVE domain containing 1 (RUFY1), transcript variant 1 |
USD 300.00 |
|
SC318189 | RUFY1 (untagged)-Human RUN and FYVE domain containing 1 (RUFY1), transcript variant 1 |
USD 714.00 |
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