DMRT3 (NM_021240) Human Tagged ORF Clone

CAT#: RC216289

DMRT3 (Myc-DDK-tagged)-Human doublesex and mab-3 related transcription factor 3 (DMRT3)

Product Images

Circular map for RC216289

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DMRT3
• Synonyms: DMRTA3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216289 representing NM_021240
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGGCTACGGCTCCCCCTACCTGTACATGGGCGGCCCGGTGTCGCAGCCGCCACGGGCGCCCCTGC
  AGCGCACGCCCAAGTGCGCGCGCTGCCGCAACCATGGCGTCCTGTCCTGGCTCAAGGGCCACAAGCGTTA
  CTGCCGCTTCAAGGACTGCACCTGCGAGAAGTGCATCCTCATCATCGAGCGGCAGCGGGTCATGGCTGCG
  CAGGTGGCGCTGCGCCGGCAGCAGGCCAACGAGAGCTTGGAGAGCCTCATCCCCGACTCGCTGCGCGCTC
  TGCCAGGGCCCCCGCCGCCGGGGGACGCCGTCGCCGCCCCGCAGCCGCCGCCAGCCTCTCAGCCGTCGCA
  GCCGCAGCCGCCGCGCCCTGCTGCCGAGTTGGCCGCGGCCGCCGCGCTGCGTTGGACTGCCGAGCCGCAG
  CCCGGGGCTCTGCAGGCGCAGCTCGCCAAGCCAGATTTGACTGAAGAACGACTTGGAGACGGCAAGTCGG
  CAGACAATACAGAGGTCTTCAGTGACAAAGACACTGACCAGAGGAGTTCCCCAGATGTGGCAAAGAGTAA
  GGGCTGCTTCACCCCTGAGAGCCCTGAGATAGTGTCCGTGGAGGAAGGGGGATACGCTGTCCAGAAAAAC
  GGAGGCAACCCCGAGAGCCGCCCTGACAGCCCCAAGTGTCACGCGGAGCAGAATCACCTCCTGATTGAGG
  GCCCCTCGGGGACTGTTTCTCTGCCCTTCAGCTTGAAAGCCAACAGACCGCCGCTTGAAGTGTTAAAAAA
  GATATTCCCCAACCAGAAGCCAACGGTGCTTGAGCTCATCCTCAAGGGCTGTGGCGGGGACCTGGTGAGC
  GCCGTGGAAGTCCTTCTGTCCAGCCGATCCTCAGTCACGGGAGCAGAGCGAACTTCCGCAGAACCTGAGA
  GTCTAGCGTTGCCCTCCAATGGGCACATCTTTGAACACACCTTGAGCTCCTACCCCATCTCGTCTTCCAA
  ATGGTCTGTGGGATCAGCCTTTCGAGTCCCAGACACGTTGAGGTTTTCTGCCGACTCTAGCAACGTTGTC
  CCCAGTCCCTTGGCTGGGCCTCTGCAGCCCCCTTTCCCCCAGCCACCCCGGTACCCGCTGATGCTGAGGA
  ATACTTTGGCGAGAAGCCAGTCGAGCCCCTTTTTGCCCAATGATGTCACCCTGTGGAACACCATGACGCT
  GCAGCAGCAGTATCAGCTGAGGTCCCAGTATGTCAGTCCTTTCCCCAGTAACTCTACCAGCGTCTTCAGA
  AGCTCGCCCGTCCTTCCTGCCCGCGCCACGGAAGACCCTCGGATTTCCATCCCTGATGATGGGTGTCCAT
  TTGTGTCAAAGCAGTCCATTTACACCGAGGACGACTATGACGAGAGGTCTGACTCCTCAGACTCTAGAAC
  ACTCAACACATCATCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216289 representing NM_021240
  Red=Cloning site Green=Tags(s)
  
  MNGYGSPYLYMGGPVSQPPRAPLQRTPKCARCRNHGVLSWLKGHKRYCRFKDCTCEKCILIIERQRVMAA
  QVALRRQQANESLESLIPDSLRALPGPPPPGDAVAAPQPPPASQPSQPQPPRPAAELAAAAALRWTAEPQ
  PGALQAQLAKPDLTEERLGDGKSADNTEVFSDKDTDQRSSPDVAKSKGCFTPESPEIVSVEEGGYAVQKN
  GGNPESRPDSPKCHAEQNHLLIEGPSGTVSLPFSLKANRPPLEVLKKIFPNQKPTVLELILKGCGGDLVS
  AVEVLLSSRSSVTGAERTSAEPESLALPSNGHIFEHTLSSYPISSSKWSVGSAFRVPDTLRFSADSSNVV
  PSPLAGPLQPPFPQPPRYPLMLRNTLARSQSSPFLPNDVTLWNTMTLQQQYQLRSQYVSPFPSNSTSVFR
  SSPVLPARATEDPRISIPDDGCPFVSKQSIYTEDDYDERSDSSDSRTLNTSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021240
• ORF Size: 1416 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021240.4
• RefSeq Size: 2184 bp
• RefSeq ORF: 1419 bp
• Locus ID: 58524
• UniProt ID: Q9NQL9
• Cytogenetics: 9p24.3
• MW: 51.2 kDa
• Gene Summary: Probable transcription factor that plays a role in configuring the spinal circuits controlling stride in vertebrates. Involved in neuronal specification within specific subdivision of spinal cord neurons and in the development of a coordinated locomotor network controlling limb movements. May regulate transcription during sexual development (By similarity).[UniProtKB/Swiss-Prot Function]