SHANK2 (NM_133266) Human Tagged ORF Clone

CAT#: RC216239

SHANK2 (Myc-DDK-tagged)-Human SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant 2

Product Images

Circular map for RC216239

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SHANK2
• Synonyms: AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK; SPANK-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216239 representing NM_133266
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGATGAACGTCCCCGGCGGAGGAGCGGCCGCGGTGATGATGACGGGCTACAATAATGGTCGCTGTC
  CCCGGAATTCTCTCTACAGTGACTGCATTATTGAGGAGAAGACGGTGGTCCTGCAGAAAAAAGACAATGA
  GGGCTTTGGATTCGTGCTTCGAGGGGCCAAAGCTGACACACCCATTGAAGAATTCACACCAACACCGGCT
  TTCCCAGCCCTACAGTACCTGGAGTCCGTGGATGAAGGTGGGGTGGCGTGGCAAGCCGGACTAAGGACCG
  GGGACTTCTTGATTGAGGTTAACAATGAGAATGTTGTCAAAGTCGGCCACAGGCAGGTGGTGAACATGAT
  CCGGCAGGGAGGGAATCACCTGGTCCTTAAGGTGGTCACGGTGACCAGGAATCTGGACCCCGACGACACC
  GCCAGGAAGAAAGCTCCCCCGCCTCCAAAGCGGGCACCGACCACAGCCCTCACCCTGCGCTCCAAGTCCA
  TGACCTCGGAGCTGGAGGAGCTCGTGGATAAAGCCTCGGTCCGGAAGAAGAAGGATAAACCCGAGGAGAT
  AGTCCCGGCCTCCAAGCCCTCCCGCGCTGCTGAGAACATGGCTGTGGAACCGAGGGTGGCGACCATCAAG
  CAGCGGCCCAGCAGCCGGTGCTTCCCGGCGGGCTCAGACATGAACTCTGTGTACGAACGCCAAGGAATCG
  CCGTGATGACGCCCACTGTTCCTGGGAGCCCAAAAGCCCCGTTTCTGGGCATCCCTCGAGGTACGATGCG
  AAGGCAGAAATCAATAGACAGCAGAATCTTTCTATCAGGAATAACAGAGGAAGAGCGGCAGTTTCTGGCT
  CCTCCAATGCTGAAGTTCACCAGAAGCCTGTCCATGCCGGACACCTCTGAGGACATCCCCCCTCCACCGC
  AGTCTGTGCCCCCGTCCCCACCACCACCTTCCCCAACCACTTACAACTGCCCCAAGTCCCCAACTCCAAG
  AGTCTACGGGACGATTAAGCCTGCGTTCAATCAGAATTCTGCCGCCAAGGTGTCCCCCGCCACCAGGTCC
  GACACCGTGGCCACCATGATGAGGGAGAAGGGGATGTACTTCAGGAGAGAGCTGGACCGCTACTCCTTGG
  ACTCTGAAGACCTCTACAGTCGGAATGCCGGCCCGCAAGCCAACTTCCGCAACAAGAGAGGCCAGATGCC
  AGAAAACCCATACTCAGAGGTGGGGAAGATCGCCAGCAAAGCCGTCTACGTCCCCGCCAAGCCCGCCAGG
  CGGAAGGGGATGCTGGTGAAGCAGTCCAACGTGGAGGACAGCCCCGAGAAGACGTGCTCCATCCCTATCC
  CGACCATCATCGTGAAGGAGCCGTCCACCAGCAGCAGCGGCAAGAGCAGCCAGGGCAGCAGCATGGAGAT
  CGACCCCCAGGCCCCGGAGCCACCGAGCCAGCTGCGGCCTGACGAAAGCCTGACCGTCAGCAGCCCCTTT
  GCCGCCGCCATCGCCGGAGCCGTCCGCGACCGTGAGAAGCGGCTGGAAGCCAGGAGGAACTCCCCGGCCT
  TCCTCTCCACAGACCTGGGGGATGAGGATGTGGGCCTGGGGCCACCCGCCCCCAGGACGCGGCCCTCCAT
  GTTCCCCGAGGAGGGGGATTTTGCTGACGAGGACAGCGCTGAGCAGCTGTCATCCCCCATGCCGAGTGCC
  ACGCCCAGGGAGCCCGAAAACCATTTCGTGGGTGGCGCCGAGGCCAGTGCTCCGGGTGAGGCTGGGAGGC
  CGCTGAATTCCACGTCCAAAGCCCAGGGGCCCGAGAGCAGCCCAGCAGTGCCCTCCGCGAGCAGCGGCAC
  AGCCGGCCCCGGGAATTATGTCCACCCACTCACAGGGCGGCTGCTTGATCCCAGCTCCCCGCTGGCCCTG
  GCACTCTCCGCAAGGGACCGAGCCATGAAGGAGTCTCAACAGGGACCCAAAGGGGAGGCCCCCAAGGCCG
  ACCTCAACAAACCTCTTTACATTGATACCAAAATGCGGCCCAGCCTGGATGCCGGCTTCCCTACGGTCAC
  CAGGCAGAACACCCGGGGACCCCTGAGGCGGCAGGAGACGGAGAACAAGTACGAGACCGACCTGGGCCGA
  GACCGGAAAGGCGATGACAAGAAGAACATGCTGATCGACATCATGGACACGTCCCAGCAGAAGTCGGCTG
  GCCTGCTGATGGTGCACACCGTGGACGCCACTAAGCTGGACAACGCCCTGCAGGAAGAGGACGAGAAGGC
  AGAGGTGGAGATGAAGCCAGACAGCTCGCCGTCCGAGGTGCCAGAAGGTGTTTCCGAAACCGAAGGTGCT
  TTACAGATCTCCGCTGCCCCCGAGCCCACCACCGTGCCCGGCAGAACCATCGTCGCGGTGGGCTCCATGG
  AAGAGGCGGTGATTTTGCCATTCCGCATCCCTCCTCCCCCTCTGGCATCCGTGGACTTGGATGAGGATTT
  TATTTTTACAGAGCCATTGCCTCCTCCCCTGGAATTTGCAAATAGTTTTGATATCCCCGATGACCGGGCA
  GCTTCTGTCCCGGCTCTCTCAGACTTAGTGAAGCAGAAGAAAAGCGACACCCCTCAGTCCCCTTCGTTGA
  ACTCCAGCCAACCAACCAACTCTGCAGACAGCAAGAAGCCAGCCAGTCTTTCAAACTGTCTGCCTGCCTC
  ATTCCTGCCACCCCCTGAAAGCTTTGACGCCGTCGCCGACTCTGGGATCGAGGAGGTGGACAGCCGGAGT
  AGCAGCGACCACCACCTCGAGACGACCAGCACTATCTCCACCGTGTCTAGCATCTCCACCCTGTCTTCCG
  AAGGTGGAGAGAATGTGGACACCTGCACAGTCTATGCAGATGGGCAAGCATTTATGGTTGACAAACCCCC
  AGTACCTCCTAAGCCAAAAATGAAGCCCATCATTCACAAAAGCAATGCACTTTATCAAGACGCGCTCGTG
  GAAGAAGATGTAGATAGCTTTGTTATCCCCCCGCCCGCTCCCCCGCCCCCGCCGGGCAGTGCCCAGCCTG
  GGATGGCCAAGGTTCTCCAGCCAAGGACCTCCAAGTTGTGGGGCGACGTCACAGAGATCAAAAGCCCGAT
  TCTCTCAGGCCCAAAGGCAAACGTTATTAGTGAATTGAACTCTATCCTACAGCAAATGAACCGAGAGAAA
  TTGGCAAAGCCGGGGGAAGGACTGGATTCACCAATGGGAGCCAAGTCCGCCAGCCTCGCTCCAAGAAGCC
  CGGAGATCATGAGCACCATCTCAGGTACACGGAGCACGACGGTCACCTTCACTGTTCGCCCCGGCACCTC
  CCAGCCCATCACCCTGCAGAGCCGGCCCCCCGACTATGAAAGCAGGACCTCAGGAACAAGACGTGCCCCA
  AGCCCTGTGGTCTCGCCAACAGAGATGAACAAAGAGACCCTGCCCGCCCCCCTGTCTGCTGCCACCGCCT
  CTCCTTCTCCCGCTCTCTCAGATGTCTTTAGCCTTCCAAGCCAGCCCCCTTCTGGGGATCTATTTGGCTT
  GAACCCAGCGGGACGCAGTAGGTCGCCATCCCCCTCGATACTGCAACAGCCAATCTCAAATAAGCCTTTT
  ACAACTAAACCTGTCCACCTGTGGACTAAACCAGATGTGGCCGATTGGCTGGAAAGTCTAAACTTGGGTG
  AACATAAAGAGGCCTTCATGGACAATGAGATCGATGGCAGTCACTTACCAAACCTGCAGAAGGAGGACCT
  CATCGATCTTGGGGTAACTCGAGTCGGGCACAGAATGAACATAGAAAGGGCTTTGAAACAGCTGCTGGAC
  AGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216239 representing NM_133266
  Red=Cloning site Green=Tags(s)
  
  MMMNVPGGGAAAVMMTGYNNGRCPRNSLYSDCIIEEKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPA
  FPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVNMIRQGGNHLVLKVVTVTRNLDPDDT
  ARKKAPPPPKRAPTTALTLRSKSMTSELEELVDKASVRKKKDKPEEIVPASKPSRAAENMAVEPRVATIK
  QRPSSRCFPAGSDMNSVYERQGIAVMTPTVPGSPKAPFLGIPRGTMRRQKSIDSRIFLSGITEEERQFLA
  PPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTTYNCPKSPTPRVYGTIKPAFNQNSAAKVSPATRS
  DTVATMMREKGMYFRRELDRYSLDSEDLYSRNAGPQANFRNKRGQMPENPYSEVGKIASKAVYVPAKPAR
  RKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSMEIDPQAPEPPSQLRPDESLTVSSPF
  AAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRTRPSMFPEEGDFADEDSAEQLSSPMPSA
  TPREPENHFVGGAEASAPGEAGRPLNSTSKAQGPESSPAVPSASSGTAGPGNYVHPLTGRLLDPSSPLAL
  ALSARDRAMKESQQGPKGEAPKADLNKPLYIDTKMRPSLDAGFPTVTRQNTRGPLRRQETENKYETDLGR
  DRKGDDKKNMLIDIMDTSQQKSAGLLMVHTVDATKLDNALQEEDEKAEVEMKPDSSPSEVPEGVSETEGA
  LQISAAPEPTTVPGRTIVAVGSMEEAVILPFRIPPPPLASVDLDEDFIFTEPLPPPLEFANSFDIPDDRA
  ASVPALSDLVKQKKSDTPQSPSLNSSQPTNSADSKKPASLSNCLPASFLPPPESFDAVADSGIEEVDSRS
  SSDHHLETTSTISTVSSISTLSSEGGENVDTCTVYADGQAFMVDKPPVPPKPKMKPIIHKSNALYQDALV
  EEDVDSFVIPPPAPPPPPGSAQPGMAKVLQPRTSKLWGDVTEIKSPILSGPKANVISELNSILQQMNREK
  LAKPGEGLDSPMGAKSASLAPRSPEIMSTISGTRSTTVTFTVRPGTSQPITLQSRPPDYESRTSGTRRAP
  SPVVSPTEMNKETLPAPLSAATASPSPALSDVFSLPSQPPSGDLFGLNPAGRSRSPSPSILQQPISNKPF
  TTKPVHLWTKPDVADWLESLNLGEHKEAFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLD
  R
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133266
• ORF Size: 3783 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133266.5
• RefSeq Size: 8872 bp
• RefSeq ORF: 3786 bp
• Locus ID: 22941
• UniProt ID: Q9UPX8
• Cytogenetics: 11q13.3-q13.4
• MW: 135.7 kDa
• Gene Summary: This gene encodes a protein that is a member of the Shank family of synaptic proteins that may function as molecular scaffolds in the postsynaptic density of excitatory synapses. Shank proteins contain multiple domains for protein-protein interaction, including ankyrin repeats, and an SH3 domain. This particular family member contains a PDZ domain, a consensus sequence for cortactin SH3 domain-binding peptides and a sterile alpha motif. The alternative splicing demonstrated in Shank genes has been suggested as a mechanism for regulating the molecular structure of Shank and the spectrum of Shank-interacting proteins in the postsynaptic densities of the adult and developing brain. Alterations in the encoded protein may be associated with susceptibility to autism spectrum disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]