FRG2 (NM_001005217) Human Tagged ORF Clone

CAT#: RC216000

FRG2 (Myc-DDK-tagged)-Human FSHD region gene 2 (FRG2)

Product Images

Circular map for RC216000

• 

  Western blot validation of overexpression lysate (Cat# LY423808) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216000 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified FRG2 protein (Cat# TP316000). The protein was produced from HEK293T cells transfected with FRG2 cDNA clone (Cat# RC216000) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FRG2
• Synonyms: FRG2A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216000 representing NM_001005217
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAAGGGAAATGAAGACTCCGATCTCCACTGCTCCTCCATCCAGTGCTCCACTGACCAGCCCCCTT
  TCCAACAGATCTCCTTTACAGAAAAGGGCTCAGATGAGAAGAAACCATTCAAAGAAAAAGGCAAGACCGC
  CTTCTCCCATTCCAGTGAGAAGCACATACAAAGGCAAGGATCGGAGCCCAATCCAAACAAGGAGAATTCT
  GAGGAAACCAAGCTCAAGGCCGGGAACAGCACTGCTGGATCAGAACCAGAGTCCAGCTCATATCGGGAAA
  ACTGCAGGAAAAGAAAAATGAGTTCCAAGGACAGCTGCCAAGACACAGCAGGGAACTGTCCAGAAAAGGA
  GTGCAGCTTGTCATTGAATAAAAAATCAAGATCCTCCACTGCTGTGCACAACAGTGAAATCCAGGAGACC
  TGTGATGCCCACCATAGGGGACATTCCAGGGCTTGCACTGGGCACAGCAAGCGGCATAGGTCTCGGGCCC
  TAGGAGTCCAAACACCGTCAATTCGAAAAAGCTTGGTGACTTCTGTGCGAGCTATGTCAGAGGCTGTTTA
  TCAAGACCTAGCCCAGGTGTGGGCACAGCAGATCCATTCTCCACTTACCTGTGAGCAGCTGACACTGCTC
  ACTCGGCTCCGGGGGCCTCTGTGTGCCCAGGTGCAGACCTTGTATTCCATGGCCACCCAGGCAGCTTATG
  TCTTCCCTGCTGAGAGCTGGCTTGTCCCAGCCACACTGCCAGGTCCTGGGGAATCAGCCCTGGATAGAGA
  AGCCCATCCCTTCCCTGGGCAGGAGATAACTGAGACTGTCAGTGGATCAGATGAGGCTAAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216000 representing NM_001005217
  Red=Cloning site Green=Tags(s)
  
  MGKGNEDSDLHCSSIQCSTDQPPFQQISFTEKGSDEKKPFKEKGKTAFSHSSEKHIQRQGSEPNPNKENS
  EETKLKAGNSTAGSEPESSSYRENCRKRKMSSKDSCQDTAGNCPEKECSLSLNKKSRSSTAVHNSEIQET
  CDAHHRGHSRACTGHSKRHRSRALGVQTPSIRKSLVTSVRAMSEAVYQDLAQVWAQQIHSPLTCEQLTLL
  TRLRGPLCAQVQTLYSMATQAAYVFPAESWLVPATLPGPGESALDREAHPFPGQEITETVSGSDEAKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005217
• ORF Size: 834 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005217.4
• RefSeq Size: 2084 bp
• RefSeq ORF: 837 bp
• Locus ID: 448831
• UniProt ID: Q64ET8
• Cytogenetics: 4q35.2
• MW: 30.3 kDa