CNKSR2 (NM_014927) Human Tagged ORF Clone

CAT#: RC215829

CNKSR2 (Myc-DDK-tagged)-Human connector enhancer of kinase suppressor of Ras 2 (CNKSR2), transcript variant 1

Product Images

Circular map for RC215829

• 

  Western blot validation of overexpression lysate (Cat# LY414934) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215829 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CNKSR2
• Synonyms: CNK2; KSR2; MAGUIN; MRXSHG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215829 representing NM_014927
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGATAATGGAACCGGTGAGCAAATGGTCTCCGAGTCAAGTAGTGGACTGGATGAAAGGTCTTG
  ATGACTGTTTGCAGCAGTATATTAAGAACTTTGAGAGGGAGAAGATCAGTGGGGACCAGCTGCTGCGCAT
  TACACATCAGGAGCTAGAAGATCTGGGGGTCAGCCGCATTGGCCATCAGGAACTGATCTTGGAAGCAGTT
  GACCTTCTGTGTGCATTGAATTATGGCTTGGAAACAGAAAATCTAAAAACCCTTTCTCACAAGTTGAATG
  CATCTGCCAAAAATCTGCAGAATTTTATAACAGGAAGGAGAAGGAGTGGCCATTATGATGGGAGGACCAG
  CCGAAAATTGCCAAACGACTTTCTGACCTCAGTTGTGGATCTGATTGGAGCAGCCAAGAGTCTGCTTGCC
  TGGTTGGACAGGTCACCATTTGCTGCTGTGACAGACTATTCAGTTACAAGAAATAATGTCATACAACTCT
  GCCTGGAGTTAACAACAATTGTGCAACAGGATTGTACTGTATATGAAACAGAGAATAAAATTCTTCACGT
  GTGTAAAACTCTTTCTGGAGTCTGTGACCACATCATATCCCTGTCGTCAGATCCTCTGGTTTCACAGTCT
  GCTCACCTGGAAGTGATTCAACTGGCAAACATTAAACCAAGCGAAGGGCTGGGTATGTATATTAAATCTA
  CATATGATGGCCTCCATGTAATTACTGGAACCACAGAAAATTCACCTGCAGATCGGTGCAAGAAAATCCA
  TGCTGGCGATGAAGTGATTCAAGTTAATCATCAGACTGTGGTGGGGTGGCAGTTGAAAAATTTGGTGAAT
  GCACTACGAGAGGACCCGAGTGGTGTTATCTTAACTTTGAAAAAGCGACCTCAGAGCATGCTTACCTCAG
  CACCAGCTTTACTGAAAAATATGAGATGGAAGCCCCTTGCTCTGCAGCCTCTTATACCTAGAAGTCCCAC
  AAGCAGCGTTGCCACGCCTTCCAGCACCATCAGTACACCCACCAAAAGAGACAGTTCTGCCCTCCAGGAT
  CTCTACATTCCCCCTCCTCCTGCAGAACCATATATTCCCAGGGATGAAAAAGGAAACCTTCCTTGTGAAG
  ACCTCAGAGGACATATGGTGGGCAAGCCAGTGCATAAGGGATCTGAATCACCAAATTCATTTCTGGATCA
  GGAATATCGAAAGAGATTTAATATTGTCGAAGAAGATACTGTCTTATATTGCTATGAATATGAAAAAGGA
  AGATCAAGTAGTCAAGGAAGACGAGAAAGCACCCCAACTTATGGCAAGCTACGACCTATATCTATGCCAG
  TGGAATATAATTGGGTGGGGGACTATGAAGATCCAAATAAGATGAAGAGAGATAGTAGAAGAGAAAACTC
  TCTACTTCGGTATATGAGCAATGAAAAGATTGCTCAAGAAGAATACATGTTTCAGAGAAACAGCAAAAAG
  GACACAGGGAAGAAGTCAAAAAAGAAGGGTGATAAGAGTAATAGCCCAACTCACTATTCATTGCTACCTA
  GTTTACAAATGGATGCACTGAGACAAGACATCATGGGCACTCCTGTGCCAGAGACCACACTATACCATAC
  ATTTCAGCAGTCCTCACTGCAGCACAAATCAAAGAAGAAAAACAAAGGTCCTATAGCAGGCAAGAGCAAA
  AGACGAATTTCTTGCAAAGATCTTGGCCGTGGTGACTGTGAGGGCTGGCTTTGGAAAAAGAAAGATGCGA
  AGAGTTACTTTTCACAGAAATGGAAAAAATATTGGTTTGTCCTAAAGGATGCATCCCTTTATTGGTATAT
  TAATGAGGAGGATGAAAAAGCAGAAGGATTCATTAGCCTGCCTGAATTTAAAATTGATAGAGCCAGTGAA
  TGCCGCAAAAAATATGCATTCAAAGCCTGTCATCCTAAAATCAAAAGCTTTTATTTTGCTGCTGAACATC
  TTGATGATATGAACAGGTGGCTTAACAGAATTAATATGCTGACTGCAGGATATGCAGAAAGAGAGAGGAT
  TAAGCAGGAACAAGATTACTGGAGTGAGAGTGACAAGGAAGAAGCAGATACTCCATCAACACCAAAACAA
  GATAGCCCTCCACCCCCATATGATACATACCCACGACCTCCCTCGATGAGTTGCGCCAGTCCTTATGTGG
  AAGCAAAACATAGCCGACTTTCCTCCACGGAGACTTCTCAGTCTCAGTCTTCTCATGAGGAGTTTCGCCA
  GGAAGTAACTGGGAGCAGTGCAGTGTCTCCCATTCGCAAGACAGCCAGTCAGCGCCGCTCCTGGCAGGAT
  TTAATTGAGACGCCACTGACAAGTTCAGGCTTACACTATCTTCAGACTCTGCCCCTGGAGGATTCTGTCT
  TCTCTGACTCCGCGGCCATCTCCCCAGAGCACAGGCGGCAGTCTACCCTGCCAACTCAGAAATGCCACCT
  GCAGGATCACTATGGGCCATACCCCTTAGCTGAGAGTGAGAGGATGCAAGTGCTAAATGGAAATGGGGGC
  AAGCCTCGAAGTTTTACTCTGCCTCGAGATAGCGGGTTCAACCATTGCTGTCTGAATGCTCCAGTTAGTG
  CCTGTGACCCACAGGATGACGTGCAACCCCCAGAGGTGGAGGAAGAGGAGGAGGAGGAGGAGGAGGAAGG
  GGAGGCAGCAGGGGAAAACATAGGAGAAAAAAGTGAAAGCAGAGAAGAAAAGTTAGGAGACTCATTGCAA
  GATTTATACAGGGCACTGGAGCAGGCCAGTCTGTCACCACTAGGAGAACATCGTATTTCAACCAAGATGG
  AATACAAGCTATCATTTATAAAAAGATGTAATGATCCTGTAATGAATGAAAAACTACACCGGCTGAGAAT
  TCTCAAAAGCACTTTAAAGGCCAGAGAAGGGGAAGTAGCCATTATCGATAAAGTCCTAGACAATCCAGAC
  TTGACATCTAAAGAATTCCAACAATGGAAGCAGATGTACCTCGACCTTTTCTTGGATATCTGTCAAAATA
  CCACCTCAAATGACCCACTGAGTATTTCTTCTGAAGTAGATGTAATCACTTCCTCTCTAGCACACACTCA
  TTCATACATTGAAACGCATGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215829 representing NM_014927
  Red=Cloning site Green=Tags(s)
  
  MALIMEPVSKWSPSQVVDWMKGLDDCLQQYIKNFEREKISGDQLLRITHQELEDLGVSRIGHQELILEAV
  DLLCALNYGLETENLKTLSHKLNASAKNLQNFITGRRRSGHYDGRTSRKLPNDFLTSVVDLIGAAKSLLA
  WLDRSPFAAVTDYSVTRNNVIQLCLELTTIVQQDCTVYETENKILHVCKTLSGVCDHIISLSSDPLVSQS
  AHLEVIQLANIKPSEGLGMYIKSTYDGLHVITGTTENSPADRCKKIHAGDEVIQVNHQTVVGWQLKNLVN
  ALREDPSGVILTLKKRPQSMLTSAPALLKNMRWKPLALQPLIPRSPTSSVATPSSTISTPTKRDSSALQD
  LYIPPPPAEPYIPRDEKGNLPCEDLRGHMVGKPVHKGSESPNSFLDQEYRKRFNIVEEDTVLYCYEYEKG
  RSSSQGRRESTPTYGKLRPISMPVEYNWVGDYEDPNKMKRDSRRENSLLRYMSNEKIAQEEYMFQRNSKK
  DTGKKSKKKGDKSNSPTHYSLLPSLQMDALRQDIMGTPVPETTLYHTFQQSSLQHKSKKKNKGPIAGKSK
  RRISCKDLGRGDCEGWLWKKKDAKSYFSQKWKKYWFVLKDASLYWYINEEDEKAEGFISLPEFKIDRASE
  CRKKYAFKACHPKIKSFYFAAEHLDDMNRWLNRINMLTAGYAERERIKQEQDYWSESDKEEADTPSTPKQ
  DSPPPPYDTYPRPPSMSCASPYVEAKHSRLSSTETSQSQSSHEEFRQEVTGSSAVSPIRKTASQRRSWQD
  LIETPLTSSGLHYLQTLPLEDSVFSDSAAISPEHRRQSTLPTQKCHLQDHYGPYPLAESERMQVLNGNGG
  KPRSFTLPRDSGFNHCCLNAPVSACDPQDDVQPPEVEEEEEEEEEEGEAAGENIGEKSESREEKLGDSLQ
  DLYRALEQASLSPLGEHRISTKMEYKLSFIKRCNDPVMNEKLHRLRILKSTLKAREGEVAIIDKVLDNPD
  LTSKEFQQWKQMYLDLFLDICQNTTSNDPLSISSEVDVITSSLAHTHSYIETHV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014927
• ORF Size: 3102 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014927.5
• RefSeq Size: 5769 bp
• RefSeq ORF: 3105 bp
• Locus ID: 22866
• UniProt ID: Q8WXI2
• Cytogenetics: Xp22.12
• Domains: PDZ, SAM, PH
• MW: 118 kDa
• Gene Summary: This gene encodes a multidomain protein that functions as a scaffold protein to mediate the mitogen-activated protein kinase pathways downstream from Ras. This gene product is induced by vitamin D and inhibits apoptosis in certain cancer cells. It may also play a role in ternary complex assembly of synaptic proteins at the postsynaptic membrane and coupling of signal transduction to membrane/cytoskeletal remodeling. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]