GRIN3A (NM_133445) Human Tagged ORF Clone
CAT#: RC215549
GRIN3A (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, N-methyl-D-aspartate 3A (GRIN3A)
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
"NM_133445" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | GRIN3A |
Synonyms | GluN3A; NMDAR-L; NMDAR3A; NR3A |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC215549 representing NM_133445
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAGACTGAGTTTGTGGTGGCTGCTGAGCAGGGTCTGTCTGCTGTTGCCGCCGCCCTGCGCACTGG TGCTGGCCGGGGTGCCCAGCTCCTCCTCGCACCCGCAGCCCTGCCAGATCCTCAAGCGCATCGGGCACGC GGTGAGGGTGGGCGCGGTGCACTTGCAGCCCTGGACCACCGCCCCCCGCGCGGCCAGCCGCGCTCCGGAC GACAGCCGAGCAGGAGCCCAGAGGGATGAGCCGGAGCCAGGGACTAGGCGGTCCCCGGCGCCCTCGCCGG GCGCACGCTGGTTGGGGAGCACCCTGCATGGCCGGGGGCCGCCGGGCTCCCGTAAGCCCGGGGAGGGCGC CAGGGCGGAGGCCCTGTGGCCACGGGACGCCCTCCTATTTGCCGTGGACAACCTGAACCGCGTGGAAGGG CTGCTACCCTACAACCTGTCTTTGGAAGTAGTGATGGCCATCGAGGCAGGCCTGGGCGATCTGCCACTTT TGCCCTTCTCCTCCCCTAGTTCGCCATGGAGCAGTGACCCTTTCTCCTTCCTGCAAAGTGTGTGCCATAC CGTGGTGGTGCAAGGGGTGTCGGCGCTGCTCGCCTTCCCCCAGAGCCAGGGCGAAATGATGGAGCTCGAC TTGGTCAGCTTAGTCCTGCACATTCCAGTGATCAGCATCGTGCGCCACGAGTTTCCACGGGAGAGTCAGA ATCCCCTTCACCTACAACTGAGTTTAGAAAATTCATTAAGTTCTGATGCTGATGTCACTGTCTCAATCCT GACCATGAACAACTGGTACAATTTTAGCTTGTTGCTGTGCCAGGAAGACTGGAACATCACCGACTTCCTC CTCCTTACCCAGAATAATTCCAAGTTCCACCTTGGTTCTATCATCAACATCACCGCTAACCTCCCCTCCA CCCAGGACCTCTTGAGCTTCCTACAGATCCAGCTTGAGAGTATTAAGAACAGCACACCCACAGTGGTGAT GTTTGGCTGCGACATGGAAAGTATCCGGCGGATTTTCGAAATTACAACCCAGTTTGGGGTCATGCCCCCT GAACTTCGTTGGGTGCTGGGAGATTCCCAGAATGTGGAGGAACTGAGGACAGAGGGTCTGCCCTTAGGGC TCATTGCTCATGGAAAAACAACACAGTCTGTCTTTGAGCACTACGTACAAGATGCTATGGAGCTGGTCGC AAGAGCTGTAGCCACAGCCACCATGATCCAACCAGAACTTGCTCTCATTCCCAGCACGATGAACTGCATG GAGGTGGAAACTACAAATCTCACTTCAGGACAATATTTATCAAGGTTTCTAGCCAATACCACTTTCAGAG GCCTCAGTGGTTCCATCAGAGTAAAAGGTTCCACCATCGTCAGCTCAGAAAACAACTTTTTCATCTGGAA TCTTCAACATGACCCCATGGGAAAGCCAATGTGGACCCGCTTGGGCAGCTGGCAGGGGGGAAAGATTGTC ATGGACTATGGAATATGGCCAGAGCAGGCCCAGAGACACAAAACCCACTTCCAACATCCAAGTAAGCTAC ACTTGAGAGTGGTTACCCTGATTGAGCATCCTTTTGTCTTCACAAGGGAGGTAGATGATGAAGGCTTGTG CCCTGCTGGCCAACTCTGTCTAGACCCCATGACTAATGACTCTTCCACATTGGACAGCCTTTTTAGCAGC CTCCATAGCAGTAATGATACAGTGCCCATTAAATTCAAGAAGTGCTGCTATGGATATTGCATTGATCTGC TGGAAAAGATAGCAGAAGACATGAACTTTGACTTCGACCTCTATATTGTAGGGGATGGAAAGTATGGAGC ATGGAAAAATGGGCACTGGACTGGGCTAGTGGGTGATCTCCTGAGAGGGACTGCCCACATGGCAGTCACT TCCTTTAGCATCAATACTGCACGGAGCCAGGTGATAGATTTCACCAGCCCTTTCTTCTCCACCAGCTTGG GCATCTTAGTGAGGACCCGAGATACAGCAGCTCCCATTGGAGCCTTCATGTGGCCACTCCACTGGACAAT GTGGCTGGGGATTTTTGTGGCTCTGCACATCACTGCCGTCTTCCTCACTCTGTATGAATGGAAGAGTCCA TTTGGTTTGACTCCCAAGGGGCGAAATAGAAGTAAAGTCTTCTCCTTTTCTTCAGCCTTGAACATCTGTT ATGCCCTCTTGTTTGGCAGAACAGTGGCCATCAAACCTCCAAAATGTTGGACTGGAAGGTTTCTAATGAA CCTTTGGGCCATTTTCTGTATGTTTTGCCTTTCCACATACACGGCAAACTTGGCTGCTGTCATGGTAGGT GAGAAGATCTATGAAGAGCTTTCTGGAATACATGACCCCAAGTTACATCATCCTTCCCAAGGATTCCGCT TTGGAACTGTCCGAGAAAGCAGTGCTGAAGATTATGTGAGACAAAGTTTCCCAGAGATGCATGAATATAT GAGAAGGTACAATGTTCCAGCCACCCCTGATGGAGTGGAGTATCTGAAGAACGATCCAGAGAAACTAGAC GCCTTCATCATGGACAAAGCCCTTCTGGATTATGAAGTGTCAATAGATGCTGACTGCAAACTTCTCACTG TGGGGAAGCCATTTGCCATAGAAGGATACGGCATTGGCCTCCCACCCAACTCTCCATTGACCGCCAACAT ATCCGAGCTAATCAGTCAATACAAGTCACATGGGTTTATGGATATGCTCCATGACAAGTGGTACAGGGTG GTTCCCTGTGGCAAGAGAAGTTTTGCTGTCACGGAGACTTTGCAAATGGGCATCAAACACTTCTCTGGGC TCTTTGTGCTGCTGTGCATTGGATTTGGTCTGTCCATTTTGACCACCATTGGTGAGCACATAGTATACAG GCTGCTGCTACCACGAATCAAAAACAAATCCAAGCTGCAATACTGGCTCCACACCAGCCAGAGATTACAC AGAGCAATAAATACATCATTTATAGAGGAAAAGCAGCAGCATTTCAAGACCAAACGTGTGGAAAAGAGGT CTAATGTGGGACCCCGTCAGCTTACCGTATGGAATACTTCCAATCTGAGTCATGACAACCGACGGAAATA CATCTTTAGTGATGAGGAAGGACAAAACCAGCTGGGCATCCAGATCCACCAGGACATCCCCCTCCCTCCA AGGAGAAGAGAGCTCCCTGCCTTGCGGACCACCAATGGGAAAGCAGACTCCCTAAATGTATCTCGGAACT CAGTGATGCAGGAACTCTCAGAGCTCGAGAAGCAGATTCAGGTGATCCGTCAGGAGCTGCAGCTGGCTGT GAGCAGGAAAACGGAGCTGGAGGAGTATCAAAGGACAAGTCGGACTTGTGAGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215549 representing NM_133445
Red=Cloning site Green=Tags(s) MRRLSLWWLLSRVCLLLPPPCALVLAGVPSSSSHPQPCQILKRIGHAVRVGAVHLQPWTTAPRAASRAPD DSRAGAQRDEPEPGTRRSPAPSPGARWLGSTLHGRGPPGSRKPGEGARAEALWPRDALLFAVDNLNRVEG LLPYNLSLEVVMAIEAGLGDLPLLPFSSPSSPWSSDPFSFLQSVCHTVVVQGVSALLAFPQSQGEMMELD LVSLVLHIPVISIVRHEFPRESQNPLHLQLSLENSLSSDADVTVSILTMNNWYNFSLLLCQEDWNITDFL LLTQNNSKFHLGSIINITANLPSTQDLLSFLQIQLESIKNSTPTVVMFGCDMESIRRIFEITTQFGVMPP ELRWVLGDSQNVEELRTEGLPLGLIAHGKTTQSVFEHYVQDAMELVARAVATATMIQPELALIPSTMNCM EVETTNLTSGQYLSRFLANTTFRGLSGSIRVKGSTIVSSENNFFIWNLQHDPMGKPMWTRLGSWQGGKIV MDYGIWPEQAQRHKTHFQHPSKLHLRVVTLIEHPFVFTREVDDEGLCPAGQLCLDPMTNDSSTLDSLFSS LHSSNDTVPIKFKKCCYGYCIDLLEKIAEDMNFDFDLYIVGDGKYGAWKNGHWTGLVGDLLRGTAHMAVT SFSINTARSQVIDFTSPFFSTSLGILVRTRDTAAPIGAFMWPLHWTMWLGIFVALHITAVFLTLYEWKSP FGLTPKGRNRSKVFSFSSALNICYALLFGRTVAIKPPKCWTGRFLMNLWAIFCMFCLSTYTANLAAVMVG EKIYEELSGIHDPKLHHPSQGFRFGTVRESSAEDYVRQSFPEMHEYMRRYNVPATPDGVEYLKNDPEKLD AFIMDKALLDYEVSIDADCKLLTVGKPFAIEGYGIGLPPNSPLTANISELISQYKSHGFMDMLHDKWYRV VPCGKRSFAVTETLQMGIKHFSGLFVLLCIGFGLSILTTIGEHIVYRLLLPRIKNKSKLQYWLHTSQRLH RAINTSFIEEKQQHFKTKRVEKRSNVGPRQLTVWNTSNLSHDNRRKYIFSDEEGQNQLGIQIHQDIPLPP RRRELPALRTTNGKADSLNVSRNSVMQELSELEKQIQVIRQELQLAVSRKTELEEYQRTSRTCES TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_133445 |
ORF Size | 3345 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_133445.1, NP_597702.1 |
RefSeq Size | 7770 bp |
RefSeq ORF | 3348 bp |
Locus ID | 116443 |
UniProt ID | Q8TCU5 |
Cytogenetics | 9q31.1 |
Protein Families | Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane |
Protein Pathways | Neuroactive ligand-receptor interaction |
MW | 125.3 kDa |
Gene Summary | This gene encodes a subunit of the N-methyl-D-aspartate (NMDA) receptors, which belong to the superfamily of glutamate-regulated ion channels, and function in physiological and pathological processes in the central nervous system. This subunit shows greater than 90% identity to the corresponding subunit in rat. Studies in the knockout mouse deficient in this subunit suggest that this gene may be involved in the development of synaptic elements by modulating NMDA receptor activity. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC215549L1 | Lenti ORF clone of Human glutamate receptor, ionotropic, N-methyl-D-aspartate 3A (GRIN3A), Myc-DDK-tagged |
USD 1,756.00 |
|
RC215549L2 | Lenti ORF clone of Human glutamate receptor, ionotropic, N-methyl-D-aspartate 3A (GRIN3A), mGFP tagged |
USD 1,756.00 |
|
RC215549L3 | Lenti ORF clone of Human glutamate receptor, ionotropic, N-methyl-D-aspartate 3A (GRIN3A), Myc-DDK-tagged |
USD 1,756.00 |
|
RC215549L4 | Lenti ORF clone of Human glutamate receptor, ionotropic, N-methyl-D-aspartate 3A (GRIN3A), mGFP tagged |
USD 1,756.00 |
|
RG215549 | GRIN3A (tGFP-tagged) - Human glutamate receptor, ionotropic, N-methyl-D-aspartate 3A (GRIN3A) |
USD 1,656.00 |
|
SC315195 | GRIN3A (untagged)-Human glutamate receptor, ionotropic, N-methyl-D-aspartate 3A (GRIN3A) |
USD 1,457.00 |
{0} Product Review(s)
Be the first one to submit a review