SPATA7 (NM_018418) Human Tagged ORF Clone

CAT#: RC215346

SPATA7 (Myc-DDK-tagged)-Human spermatogenesis associated 7 (SPATA7), transcript variant 1

Product Images

Circular map for RC215346

• 

  Western blot validation of overexpression lysate (Cat# LY413064) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215346 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SPATA7
• Synonyms: HEL-S-296; HSD-3.1; HSD3; LCA3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215346 representing NM_018418
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGGCAGCCGGAGAGTCAGAGCAACCTCTGTCCTTCCCAGATATGGTCCACCGTGCCTATTTAAAG
  GACACTTGAGCACCAAAAGTAATGCTTTTTGCACTGACTCCTCTTCTCTCAGACTAAGCACTCTCCAGCT
  GGTCAAGAATCACATGGCTGTTCACTATAATAAAATCCTTTCAGCCAAAGCTGCAGTAGACTGCTCGGTT
  CCAGTAAGCATGAGTACCAGCATAAAGTATGCAGACCAACAACGAAGAGAGAAACTCAAAAAGGAATTAG
  CACAATGTGAAAAAGAGTTCAAATTAACTAAAACTGCAATGCGAGCCAATTATAAAAATAATTCCAAGTC
  ACTTTTTAATACCTTACAAAAGCCCTCAGGCGAACCGCAAATTGAGGATGACATGTTAAAAGAAGAAATG
  AATGGATTTTCATCCTTTGCAAGGTCACTAGTACCCTCTTCAGAGAGACTACACCTAAGTCTACATAAAT
  CCAGTAAAGTCATCACAAATGGTCCTGAGAAGAACTCCAGTTCCTCCCCGTCCAGTGTGGATTATGCAGC
  CTCCGGGCCCCGGAAACTGAGCTCTGGAGCCCTGTATGGCAGAAGGCCCAGAAGCACATTCCCAAATTCC
  CACCGGTTTCAGTTAGTCATTTCGAAAGCACCCAGTGGGGATCTTTTGGATAAACATTCTGAACTCTTTT
  CTAACAAACAATTGCCATTCACTCCTCGCACTTTAAAAACAGAAGCAAAATCTTTCCTGTCACAGTATCG
  CTATTATACACCTGCCAAAAGAAAAAAGGATTTTACAGATCAACGGATAGAAGCTGAAACCCAGACTGAA
  TTAAGCTTTAAATCTGAGTTGGGGACAGCTGAGACTAAAAACATGACAGATTCAGAAATGAACATAAAGC
  AGGCATCTAATTGTGTGACATATGATGCCAAAGAAAAAATAGCTCCTTTACCTTTAGAAGGGCATGACTC
  AACATGGGATGAGATTAAGGATGATGCTCTTCAGCATTCCTCACCAAGGGCAATGTGTCAGTATTCCCTG
  AAGCCCCCTTCAACTCGTAAAATCTACTCTGATGAAGAAGAACTGTTGTATCTGAGTTTCATTGAAGATG
  TAACAGATGAAATTTTGAAACTTGGTTTATTTTCAAACAGGTTTTTAGAACGACTGTTCGAGCGACATAT
  AAAACAAAATAAACATTTGGAGGAGGAAAAAATGCGCCACCTGCTGCATGTCCTGAAAGTAGACTTAGGC
  TGCACATCGGAGGAAAACTCGGTAAAGCAAAATGATGTTGATATGTTGAATGTATTTGATTTTGAAAAGG
  CTGGGAATTCAGAACCAAATGAATTAAAAAATGAAAGTGAAGTAACAATTCAGCAGGAACGTCAACAATA
  CCAAAAGGCTTTGGATATGTTATTGTCGGCACCAAAGGATGAGAACGAGATATTCCCTTCACCAACTGAA
  TTTTTCATGCCTATTTATAAATCAAAGCATTCAGAAGGGGTTATAATTCAACAGGTGAATGATGAAACAA
  ATCTTGAAACTTCAACTTTGGATGAAAATCATCCAAGTATTTCAGACAGTTTAACAGATCGGGAAACTTC
  TGTGAATGTCATTGAAGGTGATAGTGACCCTGAAAAGGTTGAGATTTCAAATGGATTATGTGGTCTTAAC
  ACATCACCCTCCCAATCTGTTCAGTTCTCCAGTGTCAAAGGCGACAATAATCATGACATGGAGTTATCAA
  CTCTTAAAATCATGGAAATGAGCATTGAGGACTGCCCTTTGGATGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215346 representing NM_018418
  Red=Cloning site Green=Tags(s)
  
  MDGSRRVRATSVLPRYGPPCLFKGHLSTKSNAFCTDSSSLRLSTLQLVKNHMAVHYNKILSAKAAVDCSV
  PVSMSTSIKYADQQRREKLKKELAQCEKEFKLTKTAMRANYKNNSKSLFNTLQKPSGEPQIEDDMLKEEM
  NGFSSFARSLVPSSERLHLSLHKSSKVITNGPEKNSSSSPSSVDYAASGPRKLSSGALYGRRPRSTFPNS
  HRFQLVISKAPSGDLLDKHSELFSNKQLPFTPRTLKTEAKSFLSQYRYYTPAKRKKDFTDQRIEAETQTE
  LSFKSELGTAETKNMTDSEMNIKQASNCVTYDAKEKIAPLPLEGHDSTWDEIKDDALQHSSPRAMCQYSL
  KPPSTRKIYSDEEELLYLSFIEDVTDEILKLGLFSNRFLERLFERHIKQNKHLEEEKMRHLLHVLKVDLG
  CTSEENSVKQNDVDMLNVFDFEKAGNSEPNELKNESEVTIQQERQQYQKALDMLLSAPKDENEIFPSPTE
  FFMPIYKSKHSEGVIIQQVNDETNLETSTLDENHPSISDSLTDRETSVNVIEGDSDPEKVEISNGLCGLN
  TSPSQSVQFSSVKGDNNHDMELSTLKIMEMSIEDCPLDV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018418
• ORF Size: 1797 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018418.5
• RefSeq Size: 2009 bp
• RefSeq ORF: 1800 bp
• Locus ID: 55812
• UniProt ID: Q9P0W8
• Cytogenetics: 14q31.3
• MW: 67.5 kDa
• Gene Summary: This gene, originally isolated from testis, is also expressed in retina. Mutations in this gene are associated with Leber congenital amaurosis and juvenile retinitis pigmentosa. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]