EEF1AKMT4-ECE2 (NM_014693) Human Tagged ORF Clone
CAT#: RC214847
ECE2 (Myc-DDK-tagged)-Human endothelin converting enzyme 2 (ECE2), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_014693" in other vectors (5)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | EEF1AKMT4-ECE2 |
Synonyms | ECE-2; ECE2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC214847 representing NM_014693
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCTCCAGGGGCAGGTAGGGCGCCTCCGGAGTTACCGGAGCGGAACTGCGGGTACCGCGAAGTCG AGTACTGGGATCAGCGCTACCAAGGCGCAGCCGATTCTGCCCCCTACGATTGGTTCGGGGACTTCTCCTC CTTCCGTGCCCTCCTAGAGCCGGAGCTGCGGCCCGAGGACCGTATCCTTGTGCTAGGTTGCGGGAACAGT GCCCTGAGCTACGAGCTGTTCCTCGGAGGCTTCCCTAATGTGACCAGTGTGGACTACTCATCAGTCGTGG TGGCTGCCATGCAGGCTCGCCATGCCCATGTGCCGCAGCTGCGCTGGGAGACCATGGATGTGCGGAAGCT GGACTTCCCCAGTGCTTCTTTTGATGTGGTGCTCGAGAAGGGCACGCTGGATGCCCTGCTGGCTGGGGAA CGAGATCCCTGGACCGTGTCCTCTGAAGGTGTCCACACTGTGGACCAGGTGTTGAGTGAGGTGGGATTCC AGAAGGGGACAAGACAGCTGTTAGGCTCACGCACGCAGCTGGAGCTGGTCTTAGCAGGTGCCTCTCTACT GCTGGCTGCACTGCTTCTGGGCTGCCTTGTGGCCCTAGGGGTCCAGTACCACAGAGACCCATCCCACAGC ACCTGCCTTACAGAGGCCTGCATTCGAGTGGCTGGAAAAATCCTGGAGTCCCTGGACCGAGGGGTGAGCC CCTGTGAGGACTTTTACCAGTTCTCCTGTGGGGGCTGGATTCGGAGGAACCCCCTGCCCGATGGGCGTTC TCGCTGGAACACCTTCAACAGCCTCTGGGACCAAAACCAGGCCATACTGAAGCACCTGCTTGAAAACACC ACCTTCAACTCCAGCAGTGAAGCTGAGCAGAAGACACAGCGCTTCTACCTATCTTGCCTACAGGTGGAGC GCATTGAGGAGCTGGGAGCCCAGCCACTGAGAGACCTCATTGAGAAGATTGGTGGTTGGAACATTACGGG GCCCTGGGACCAGGACAACTTTATGGAGGTGTTGAAGGCAGTAGCAGGGACCTACAGGGCCACCCCATTC TTCACCGTCTACATCAGTGCCGACTCTAAGAGTTCCAACAGCAATGTTATCCAGGTGGACCAGTCTGGGC TCTTTCTGCCCTCTCGGGATTACTACTTAAACAGAACTGCCAATGAGAAAGTGCTCACTGCCTATCTGGA TTACATGGAGGAACTGGGGATGCTGCTGGGTGGGCGGCCCACCTCCACGAGGGAGCAGATGCAGCAGGTG CTGGAGTTGGAGATACAGCTGGCCAACATCACAGTGCCCCAGGACCAGCGGCGCGACGAGGAGAAGATCT ACCACAAGATGAGCATTTCGGAGCTGCAGGCTCTGGCGCCCTCCATGGACTGGCTTGAGTTCCTGTCTTT CTTGCTGTCACCATTGGAGTTGAGTGACTCTGAGCCTGTGGTGGTGTATGGGATGGATTATTTGCAGCAG GTGTCAGAGCTCATCAACCGCACGGAACCAAGCATCCTGAACAATTACCTGATCTGGAACCTGGTGCAAA AGACAACCTCAAGCCTGGACCGACGCTTTGAGTCTGCACAAGAGAAGCTGCTGGAGACCCTCTATGGCAC TAAGAAGTCCTGTGTGCCGAGGTGGCAGACCTGCATCTCCAACACGGATGACGCCCTTGGCTTTGCTTTG GGGTCCCTCTTCGTGAAGGCCACGTTTGACCGGCAAAGCAAAGAAATTGCAGAGGGGATGATCAGCGAAA TCCGGACCGCATTTGAGGAGGCCCTGGGACAGCTGGTTTGGATGGATGAGAAGACCCGCCAGGCAGCCAA GGAGAAAGCAGATGCCATCTATGATATGATTGGTTTCCCAGACTTTATCCTGGAGCCCAAAGAGCTGGAT GATGTTTATGACGGGTACGAAATTTCTGAAGATTCTTTCTTCCAAAACATGTTGAATTTGTACAACTTCT CTGCCAAGGTTATGGCTGACCAGCTCCGCAAGCCTCCCAGCCGAGACCAGTGGAGCATGACCCCCCAGAC AGTGAATGCCTACTACCTTCCAACTAAGAATGAGATCGTCTTCCCCGCTGGCATCCTGCAGGCCCCCTTC TATGCCCGCAACCACCCCAAGGCCCTGAACTTCGGTGGCATCGGTGTGGTCATGGGCCATGAGTTGACGC ATGCCTTTGATGACCAAGGGCGCGAGTATGACAAAGAAGGGAACCTGCGGCCCTGGTGGCAGAATGAGTC CCTGGCAGCCTTCCGGAACCACACGGCCTGCATGGAGGAACAGTACAATCAATACCAGGTCAATGGGGAG AGGCTCAACGGCCGCCAGACGCTGGGGGAGAACATTGCTGACAACGGGGGGCTGAAGGCTGCCTACAATG CTTACAAAGCATGGCTGAGAAAGCATGGGGAGGAGCAGCAACTGCCAGCCGTGGGGCTCACCAACCACCA GCTCTTCTTCGTGGGATTTGCCCAGGTGTGGTGCTCGGTCCGCACACCAGAGAGCTCTCACGAGGGGCTG GTGACCGACCCCCACAGCCCTGCCCGCTTCCGCGTGCTGGGCACTCTCTCCAACTCCCGTGACTTCCTGC GGCACTTCGGCTGCCCTGTCGGCTCCCCCATGAACCCAGGGCAGCTGTGTGAGGTGTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214847 representing NM_014693
Red=Cloning site Green=Tags(s) MASPGAGRAPPELPERNCGYREVEYWDQRYQGAADSAPYDWFGDFSSFRALLEPELRPEDRILVLGCGNS ALSYELFLGGFPNVTSVDYSSVVVAAMQARHAHVPQLRWETMDVRKLDFPSASFDVVLEKGTLDALLAGE RDPWTVSSEGVHTVDQVLSEVGFQKGTRQLLGSRTQLELVLAGASLLLAALLLGCLVALGVQYHRDPSHS TCLTEACIRVAGKILESLDRGVSPCEDFYQFSCGGWIRRNPLPDGRSRWNTFNSLWDQNQAILKHLLENT TFNSSSEAEQKTQRFYLSCLQVERIEELGAQPLRDLIEKIGGWNITGPWDQDNFMEVLKAVAGTYRATPF FTVYISADSKSSNSNVIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMEELGMLLGGRPTSTREQMQQV LELEIQLANITVPQDQRRDEEKIYHKMSISELQALAPSMDWLEFLSFLLSPLELSDSEPVVVYGMDYLQQ VSELINRTEPSILNNYLIWNLVQKTTSSLDRRFESAQEKLLETLYGTKKSCVPRWQTCISNTDDALGFAL GSLFVKATFDRQSKEIAEGMISEIRTAFEEALGQLVWMDEKTRQAAKEKADAIYDMIGFPDFILEPKELD DVYDGYEISEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWSMTPQTVNAYYLPTKNEIVFPAGILQAPF YARNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWWQNESLAAFRNHTACMEEQYNQYQVNGE RLNGRQTLGENIADNGGLKAAYNAYKAWLRKHGEEQQLPAVGLTNHQLFFVGFAQVWCSVRTPESSHEGL VTDPHSPARFRVLGTLSNSRDFLRHFGCPVGSPMNPGQLCEVW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_014693 |
ORF Size | 2649 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014693.4 |
RefSeq Size | 3498 bp |
RefSeq ORF | 2652 bp |
Locus ID | 110599583 |
UniProt ID | P0DPD6 |
Cytogenetics | 3q27.1 |
Domains | Peptidase_M13 |
MW | 99.6 kDa |
Gene Summary | This gene represents naturally occurring readthrough transcription between adjacent genes eukaryotic translation elongation factor 1 alpha lysine methyltransferase 4 (GeneID: 110599564) and endothelin converting enzyme 2 (GeneID:9718). The readthrough transcript representing this gene encodes a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq, Jul 2017] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC214847L3 | Lenti ORF clone of Human endothelin converting enzyme 2 (ECE2), transcript variant 1, Myc-DDK-tagged |
USD 1,108.00 |
|
RC214847L4 | Lenti ORF clone of Human endothelin converting enzyme 2 (ECE2), transcript variant 1, mGFP tagged |
USD 1,108.00 |
|
RG214847 | ECE2 (tGFP-tagged) - Human endothelin converting enzyme 2 (ECE2), transcript variant 1 |
USD 1,008.00 |
|
SC310113 | ECE2 (untagged)-Human endothelin converting enzyme 2 (ECE2), transcript variant 1 |
USD 722.00 |
|
SC318357 | ECE2 (untagged)-Human endothelin converting enzyme 2 (ECE2), transcript variant 1 |
USD 890.00 |
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