CCDC85A (NM_001080433) Human Tagged ORF Clone

CAT#: RC214649

CCDC85A (Myc-DDK-tagged)-Human coiled-coil domain containing 85A (CCDC85A)

Product Images

Circular map for RC214649

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  Western blot validation of overexpression lysate (Cat# LY421650) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214649 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CCDC85A protein (Cat# TP314649). The protein was produced from HEK293T cells transfected with CCDC85A cDNA clone (Cat# RC214649) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CCDC85A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214649 representing NM_001080433
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGAAGGCGGCCGGAGGCGCGGCGGCGGCTGCGGCGGCGGCGGAAAGTTGTTCCCCAGCCCCGGCCG
  GCTCGTCCGCGGCCCCGCCCGCGCCGGTGGAGGACCTGTCCAAAGTGTCGGACGAGGAGCTGCTGCAGTG
  GAGCAAGGAGGAGCTGATCCGCAGCCTGCGGCGCGCCGAGGCGGAGAAGGTGAGCGCGATGCTGGACCAC
  AGCAACCTCATCCGCGAGGTGAACCGCCGCCTGCAGCTGCACCTCGGCGAGATCCGCGGCCTCAAGGATA
  TCAACCAGAAACTCCAGGAAGACAACCAGGAACTGAGGGACCTCTGCTGTTTCCTGGATGATGACCGGCA
  GAAAGGCAAGAGGGTGTCTCGGGAGTGGCAGAGACTGGGTCGCTACACTGCCGGGGTGATGCACAAGGAA
  GTGGCCTTATACCTGCAGAAGCTGAAAGACCTGGAGGTGAAGCAGGAGGAAGTGGTGAAGGAGAACATGG
  AGCTCAAGGAGCTCTGTGTGCTACTAGATGAGGAGAAGGGTGCAGGCTGCGCAGGCAGCCGCTGCTCCAT
  CGACAGCCAGGCCAGCCTGTGCCAACTCACAGCCTCCACCGCACCCTACGTGCGGGATGTGGGTGACGGC
  AGCAGCACCTCCAGCACTGGCAGCACTGACAGCCCGGACCACCACAAGCACCACGCGAGCAGTGGCAGCC
  CGGAGCACCTGCAGAAGCCCCGGAGCGAGGGCAGCCCGGAGCACTCCAAGCACAGGAGCGCCAGCCCCGA
  GCATCCACAGAAACCCAGAGCCTGTGGAACCCCAGATCGCCCCAAAGCACTCAAAGGACCTAGCCCGGAG
  CACCACAAACCCTTGTGCAAGGGCAGCCCCGAACAGCAAAGGCACCCGCATCCAGGGAGCAGCCCCGAAA
  CGCTGCCCAAGCACGTGCTGAGTGGGAGCCCGGAACACTTCCAGAAGCACCGGTCAGGGAGCAGCCCTGA
  ACACGCCAGGCACAGTGGAGGGAGCCCGGAGCATCTTCAGAAACACGCTCTTGGGGGGAGCCTAGAGCAT
  CTCCCCAGAGCCAGGGGCACCAGCCCGGAGCACCTCAAACAACATTATGGAGGGAGCCCTGATCACAAAC
  ACGGAGGAGGCAGTGGAGGAAGTGGAGGCAGCGGCGGAGGCAGCAGGGAGGGCACCCTCAGACGGCAGGC
  ACAGGAGGACGGGTCACCCCATCACCGGAATGTCTACAGTGGCATGAACGAATCAACCTTGTCCTATGTT
  AGGCAGCTGGAGGCAAGAGTAAGACAGCTGGAGGAAGAAAATCGCATGCTGCCCCAGGCCAGCCAGAATA
  GAAGGCAACCTCCAACTAGAAACAGCTCAAATATGGAGAAAGGCTGGGGGTCCAGAGCCCGGCGGGTCTT
  GCAGTGGTGGCAAGGGTGCCGAGGAATAGGACGATGCCTGCCTACTCTCCCGGGTTCTTTTAGGTTGTCA
  TCAGGGGCTGATGGGAGTAACAGTTCACCCAACTCTGCAGCTAGCTTCAGTGGACATGCCACACCTTCCC
  AGCAGCCTGAACCTGTGGTACATTCTCTTAAGGTTGTGTGGAGGAAACTTGGAGATGCTGCAGGTTCGTG
  TCCTGGAATTAGGCAACATTTGTCAGGAAACCAGTACAAAGGACCAATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214649 representing NM_001080433
  Red=Cloning site Green=Tags(s)
  
  MSKAAGGAAAAAAAAESCSPAPAGSSAAPPAPVEDLSKVSDEELLQWSKEELIRSLRRAEAEKVSAMLDH
  SNLIREVNRRLQLHLGEIRGLKDINQKLQEDNQELRDLCCFLDDDRQKGKRVSREWQRLGRYTAGVMHKE
  VALYLQKLKDLEVKQEEVVKENMELKELCVLLDEEKGAGCAGSRCSIDSQASLCQLTASTAPYVRDVGDG
  SSTSSTGSTDSPDHHKHHASSGSPEHLQKPRSEGSPEHSKHRSASPEHPQKPRACGTPDRPKALKGPSPE
  HHKPLCKGSPEQQRHPHPGSSPETLPKHVLSGSPEHFQKHRSGSSPEHARHSGGSPEHLQKHALGGSLEH
  LPRARGTSPEHLKQHYGGSPDHKHGGGSGGSGGSGGGSREGTLRRQAQEDGSPHHRNVYSGMNESTLSYV
  RQLEARVRQLEEENRMLPQASQNRRQPPTRNSSNMEKGWGSRARRVLQWWQGCRGIGRCLPTLPGSFRLS
  SGADGSNSSPNSAASFSGHATPSQQPEPVVHSLKVVWRKLGDAAGSCPGIRQHLSGNQYKGPM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080433
• ORF Size: 1659 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080433.2
• RefSeq Size: 3983 bp
• RefSeq ORF: 1662 bp
• Locus ID: 114800
• UniProt ID: Q96PX6
• Cytogenetics: 2p16.1
• MW: 59.8 kDa
• Gene Summary: May play a role in cell-cell adhesion and epithelium development through its interaction with proteins of the beta-catenin family.[UniProtKB/Swiss-Prot Function]