SAMD9L (NM_152703) Human Tagged ORF Clone

CAT#: RC214427

SAMD9L (Myc-DDK-tagged)-Human sterile alpha motif domain containing 9-like (SAMD9L)

Product Images

Circular map for RC214427

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SAMD9L
• Synonyms: ATXPC; C7orf6; DRIF2; M7MLS1; UEF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214427 representing NM_152703
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAAACAAGTATCTCTACCTGAAATGATTAAAGACTGGACCAAAGAGCATGTGAAAAAATGGGTAA
  ATGAAGACCTTAAGATTAATGAGCAATACGGGCAAATTCTGCTCAGTGAAGAAGTAACAGGATTAGTCCT
  GCAGGAATTAACTGAGAAGGACCTTGTAGAAATGGGGCTACCATGGGGTCCAGCACTTTTGATAAAACGT
  TCATACAACAAATTGAATAGTAAGTCCCCTGAAAGTGACAATCATGATCCGGGACAATTAGATAATTCAA
  AACCGTCCAAAACAGAACACCAGAAAAATCCAAAACACACCAAAAAGGAAGAAGAAAATTCAATGTCATC
  TAATATTGATTATGATCCCAGAGAGATCAGAGATATCAAACAAGAAGAATCAATTCTTATGAAAGAAAAT
  GTGTTAGATGAAGTAGCAAATGCTAAACACAAGAAAAAGGGTAAGCTAAAACCTGAACAATTGACTTGTA
  TGCCATATCCTTTTGATCAGTTCCATGACAGCCATCGCTACATAGAACATTATACTCTACAACCTGAAAC
  AGGAGCACTCAATCTCATTGATCCAATACATGAGTTCAAAGCTCTCACAAACACAGAAACAGCCACGGAA
  GTGGACATTAAGATGAAATTCAGCAATGAAGTCTTCCGATTTGCATCAGCTTGTATGAATTCACGCACCA
  ATGGCACCATCCATTTTGGAGTCAAGGACAAACCCCATGGAGAAATTGTTGGTGTGAAAATCACCAGTAA
  GGCTGCCTTCATTGACCACTTCAATGTAATGATCAAAAAGTATTTTGAAGAAAGTGAGATCAATGAAGCC
  AAGAAGTGTATTCGGGAGCCAAGGTTTGTGGAAGTCCTTCTGCAGAACAATACACCATCTGACAGATTTG
  TCATTGAAGTTGATACTATTCCAAAACACTCTATATGTAATGATAAGTATTTCTACATTCAGATGCAAAT
  TTGTAAAGATAAAATATGGAAACAAAACCAAAATCTTTCACTGTTTGTAAGAGAAGGGGCTAGCTCTAGG
  GATATCCTGGCCAATTCCAAGCAACGGGATGTAGATTTCAAGGCATTTTTACAAAATTTAAAGTCACTGG
  TAGCATCTAGAAAAGAGGCTGAAGAAGAGTATGGAATGAAGGCAATGAAGAAGGAGAGTGAAGGACTAAA
  GCTGGTTAAACTTCTCATAGGAAACCGAGACTCACTGGATAATTCATACTATGACTGGTACATTCTTGTA
  ACAAATAAATGCCATCCAAACCAAATAAAGCACTTAGATTTTTTAAAAGAAATTAAATGGTTTGCTGTGT
  TGGAGTTTGATCCTGAATCTATGATCAATGGAGTGGTCAAAGCTTACAAAGAAAGTCGGGTGGCAAACCT
  TCACTTTCCAAATCAATATGAAGACAAGACAACTAACATGTGGGAGAAGATTTCTACTCTTAATCTTTAC
  CAACAGCCCAGCTGGATTTTCTGCAACGGCAGATCAGACCTGAAAAGCGAGACATATAAACCTCTAGAAC
  CACATTTATGGCAGAGAGAAAGAGCTTCAGAAGTCAGGAAACTAATTTTATTTCTCACAGATGAAAATAT
  AATGACAAGAGGAAAATTTTTGGTAGTGTTTCTATTACTCTCTTCAGTGGAAAGCCCAGGAGATCCACTC
  ATTGAAACTTTCTGGGCTTTCTATCAAGCTCTCAAAGGAATGGAAAATATGTTGTGTATCTCTGTAAACT
  CACATATTTATCAACGATGGAAAGATCTACTACAAACAAGAATGAAGATGGAAGATGAACTAACAAACCA
  CAGTATTTCCACTTTAAATATAGAACTGGTAAACAGCACTATCCTTAAACTAAAATCGGTGACTCGGTCA
  TCAAGAAGGTTTTTGCCCGCCCGTGGATCTTCTTCAGTTATCCTAGAGAAAAAGAAAGAGGATGTCTTGA
  CTGCACTGGAAATCCTCTGTGAAAATGAGTGTACAGAGACAGACATCGAGAAAGACAAATCTAAATTCCT
  GGAGTTTAAGAAATCAAAAGAAGAACACTTTTATCGAGGTGGCAAAGTATCCTGGTGGAACTTCTATTTT
  TCTTCTGAAAACTATTCTTCAGATTTTGTTAAAAGGGACAGTTATGAAAAGCTTAAAGATTTAATACACT
  GCTGGGCAGAGTCTCCTAAACCAATATTTGCAAAAATCATCAATCTTTATCATCATCCAGGCTGTGGAGG
  TACCACACTGGCTATGCATGTTCTCTGGGACTTAAAGAAAAACTTCAGATGTGCTGTGTTAAAAAACAAG
  ACAACTGATTTTGCAGAAATTGCAGAGCAAGTGATCAATCTGGTCACCTATAGGGCAAAGAGCCATCAGG
  ATTACATTCCTGTGCTTCTCCTTGTGGATGATTTTGAAGAACAAGAAAATGTCTACTTTCTACAAAATGC
  CATCCATTCCGTTTTAGCAGAAAAGGATTTGCGATATGAAAAAACATTGGTAATTATCTTAAACTGCATG
  AGATCCCGGAATCCAGATGAAAGTGCAAAATTGGCAGACAGTATTGCACTAAATTACCAACTTTCTTCCA
  AGGAACAAAGAGCTTTTGGTGCCAAACTGAAGGAAATTGAAAAGCAGCACAAGAACTGTGAAAACTTTTA
  TTCCTTCATGATCATGAAAAGCAATTTTGATGAAACATATATAGAAAATGTAGTCAGGAATATCCTAAAA
  GGACAGGATGTTGACAGCAAGGAAGCACAACTCATTTCCTTCCTGGCTTTACTCAGCTCTTATGTTACTG
  ACTCTACAATTTCAGTTTCACAGTGTGAAATATTTTTGGGAATCATATACACTAGTACACCCTGGGAACC
  TGAAAGCTTAGAAGACAAGATGGGAACTTATTCTACACTTCTAATAAAAACAGAAGTTGCAGAATATGGG
  AGATACACAGGTGTGCGTATCATTCACCCTCTGATTGCCCTGTACTGTCTAAAAGAACTGGAAAGAAGCT
  ATCACTTGGATAAATGTCAAATTGCATTGAATATATTAGAAGAGAATTTATTCTATGATTCTGGAATAGG
  AAGAGACAAATTTCAACATGATGTTCAAACTCTTCTGCTTACAAGACAGCGCAAGGTGTATGGAGATGAA
  ACAGACACTCTGTTTTCCCCATTAATGGAAGCTTTACAGAATAAAGACATTGAAAAGGTCTTGAGTGCAG
  GAAGTAGACGATTCCCACAAAATGCATTCATTTGTCAAGCCTTAGCAAGACATTTCTACATTAAAGAGAA
  GGACTTTAACACAGCTCTGGACTGGGCACGTCAGGCCAAAATGAAAGCACCTAAAAATTCCTATATTTCA
  GATACACTAGGTCAAGTCTACAAAAGTGAAATCAAATGGTGGTTGGATGGGAACAAAAACTGTAGGAGCA
  TTACTGTTAATGACCTAACACATCTCCTAGAAGCTGCGGAAAAAGCCTCAAGAGCTTTCAAAGAATCCCA
  AAGGCAAACTGATAGTAAAAACTATGAAACCGAGAACTGGTCACCACAGAAGTCCCAGAGACGATATGAC
  ATGTATAACACAGCTTGTTTCTTGGGTGAAATAGAAGTTGGTCTTTACACTATCCAGATTCTTCAGCTCA
  CTCCCTTTTTCCACAAAGAAAATGAATTATCCAAAAAACATATGGTGCAATTTTTATCAGGAAAGTGGAC
  CATTCCTCCTGATCCCAGAAATGAATGTTATTTGGCTCTTAGCAAGTTCACATCCCACCTAAAAAATTTA
  CAATCAGATCTGAAAAGGTGCTTTGACTTTTTTATTGATTATATGGTTCTTCTGAAAATGAGGTATACCC
  AAAAAGAAATTGCAGAAATCATGTTAAGCAAGAAAGTCAGTCGTTGTTTCAGGAAATACACAGAACTTTT
  CTGTCATTTGGATCCATGTCTATTACAAAGTAAAGAGAGTCAATTACTCCAGGAGGAGAATTGCAGGAAA
  AAGCTAGAAGCTCTGAGAGCAGATAGGTTTGCTGGACTCTTGGAATATCTTAATCCAAACTACAAAGATG
  CTACCACCATGGAAAGTATAGTGAATGAATATGCCTTCCTACTGCAGCAAAACTCAAAAAAGCCCATGAC
  AAATGAGAAACAAAATTCCATTTTGGCCAACATTATTCTGAGTTGTCTAAAGCCCAACTCCAAGTTAATT
  CAACCACTTACCACGCTAAAAAAACAACTCCGAGAGGTCTTGCAATTTGTAGGACTAAGTCATCAATATC
  CAGGTCCTTATTTCTTGGCCTGCCTCCTGTTCTGGCCAGAAAATCAAGAGCTAGATCAAGATTCCAAACT
  AATAGAAAAGTATGTTTCATCCTTAAATAGATCCTTCAGGGGACAGTACAAGCGCATGTGCAGGTCCAAG
  CAGGCAAGCACACTTTTCTATCTGGGCAAAAGGAAGGGTCTAAACAGTATTGTTCACAAGGCCAAAATAG
  AGCAGTACTTTGATAAAGCACAAAATACAAATTCCCTCTGGCACAGTGGGGATGTGTGGAAAAAAAATGA
  AGTCAAAGACCTCCTGCGTCGTCTAACTGGTCAGGCTGAAGGCAAGCTAATCTCTGTAGAATATGGAACA
  GAGGAAAAAATAAAAATACCAGTAATATCTGTTTATTCAGGTCCACTCAGAAGTGGTAGGAACATAGAAA
  GAGTGTCTTTCTACCTAGGATTTTCCATTGAAGGCCCTCTGGCATATGATATAGAAGTAATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214427 representing NM_152703
  Red=Cloning site Green=Tags(s)
  
  MSKQVSLPEMIKDWTKEHVKKWVNEDLKINEQYGQILLSEEVTGLVLQELTEKDLVEMGLPWGPALLIKR
  SYNKLNSKSPESDNHDPGQLDNSKPSKTEHQKNPKHTKKEEENSMSSNIDYDPREIRDIKQEESILMKEN
  VLDEVANAKHKKKGKLKPEQLTCMPYPFDQFHDSHRYIEHYTLQPETGALNLIDPIHEFKALTNTETATE
  VDIKMKFSNEVFRFASACMNSRTNGTIHFGVKDKPHGEIVGVKITSKAAFIDHFNVMIKKYFEESEINEA
  KKCIREPRFVEVLLQNNTPSDRFVIEVDTIPKHSICNDKYFYIQMQICKDKIWKQNQNLSLFVREGASSR
  DILANSKQRDVDFKAFLQNLKSLVASRKEAEEEYGMKAMKKESEGLKLVKLLIGNRDSLDNSYYDWYILV
  TNKCHPNQIKHLDFLKEIKWFAVLEFDPESMINGVVKAYKESRVANLHFPNQYEDKTTNMWEKISTLNLY
  QQPSWIFCNGRSDLKSETYKPLEPHLWQRERASEVRKLILFLTDENIMTRGKFLVVFLLLSSVESPGDPL
  IETFWAFYQALKGMENMLCISVNSHIYQRWKDLLQTRMKMEDELTNHSISTLNIELVNSTILKLKSVTRS
  SRRFLPARGSSSVILEKKKEDVLTALEILCENECTETDIEKDKSKFLEFKKSKEEHFYRGGKVSWWNFYF
  SSENYSSDFVKRDSYEKLKDLIHCWAESPKPIFAKIINLYHHPGCGGTTLAMHVLWDLKKNFRCAVLKNK
  TTDFAEIAEQVINLVTYRAKSHQDYIPVLLLVDDFEEQENVYFLQNAIHSVLAEKDLRYEKTLVIILNCM
  RSRNPDESAKLADSIALNYQLSSKEQRAFGAKLKEIEKQHKNCENFYSFMIMKSNFDETYIENVVRNILK
  GQDVDSKEAQLISFLALLSSYVTDSTISVSQCEIFLGIIYTSTPWEPESLEDKMGTYSTLLIKTEVAEYG
  RYTGVRIIHPLIALYCLKELERSYHLDKCQIALNILEENLFYDSGIGRDKFQHDVQTLLLTRQRKVYGDE
  TDTLFSPLMEALQNKDIEKVLSAGSRRFPQNAFICQALARHFYIKEKDFNTALDWARQAKMKAPKNSYIS
  DTLGQVYKSEIKWWLDGNKNCRSITVNDLTHLLEAAEKASRAFKESQRQTDSKNYETENWSPQKSQRRYD
  MYNTACFLGEIEVGLYTIQILQLTPFFHKENELSKKHMVQFLSGKWTIPPDPRNECYLALSKFTSHLKNL
  QSDLKRCFDFFIDYMVLLKMRYTQKEIAEIMLSKKVSRCFRKYTELFCHLDPCLLQSKESQLLQEENCRK
  KLEALRADRFAGLLEYLNPNYKDATTMESIVNEYAFLLQQNSKKPMTNEKQNSILANIILSCLKPNSKLI
  QPLTTLKKQLREVLQFVGLSHQYPGPYFLACLLFWPENQELDQDSKLIEKYVSSLNRSFRGQYKRMCRSK
  QASTLFYLGKRKGLNSIVHKAKIEQYFDKAQNTNSLWHSGDVWKKNEVKDLLRRLTGQAEGKLISVEYGT
  EEKIKIPVISVYSGPLRSGRNIERVSFYLGFSIEGPLAYDIEVI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152703
• ORF Size: 4752 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152703.5
• RefSeq Size: 7133 bp
• RefSeq ORF: 4755 bp
• Locus ID: 219285
• UniProt ID: Q8IVG5
• Cytogenetics: 7q21.2
• MW: 184.4 kDa
• Gene Summary: This gene encodes a cytoplasmic protein that acts as a tumor suppressor but also plays a key role in cell proliferation and the innate immune response to viral infection. The encoded protein contains an N-terminal sterile alpha motif domain. Naturally occurring mutations in this gene are associated with myeloid disorders such as juvenile myelomonocytic leukemia, acute myeloid leukemia, and myelodysplastic syndrome. Naturally occurring mutations are also associated with hepatitis-B related hepatocellular carcinoma, normophosphatemic familial tumoral calcinosis, and ataxia-pancytopenia syndrome. [provided by RefSeq, Apr 2017]