Hemogen (HEMGN) (NM_197978) Human Tagged ORF Clone

CAT#: RC214122

HEMGN (Myc-DDK-tagged)-Human hemogen (HEMGN), transcript variant 2

Product Images

Circular map for RC214122

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  Western blot validation of overexpression lysate (Cat# LY405099) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214122 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified HEMGN protein (Cat# TP314122). The protein was produced from HEK293T cells transfected with HEMGN cDNA clone (Cat# RC214122) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hemogen
• Synonyms: CT155; EDAG; EDAG-1; NDR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214122 representing NM_197978.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGATTTGGGAAAGGACCAATCTCATTTGAAGCACCATCAGACACCTGACCCTCATCAAGAAGAGAAC
  CATTCTCCAGAAGTCATTGGAACCTGGAGTTTGAGAAACAGAGAACTACTTAGAAAAAGAAAAGCTGAA
  GTGCATGAAAAGGAAACATCACAATGGCTATTTGGAGAACAGAAAAAACGCAAGCAGCAGAGAACAGGA
  AAAGGAAATCGAAGAGGCAGAAAGAGACAACAAAACACAGAATTGAAGGTGGAGCCTCAGCCACAGATA
  GAAAAGGAAATGGAGAAAGCACTGGCACCTATAGAGAAAAAAACTGAGCCACCTGGGAGCATAACCAAA
  GTATTTCCTTCAGTAGCCTCCCCGCAAAAAGTTGTGCCTGAGGAACACTTTTCTGAAATATGTCAAGAA
  AGTAACATATATCAGGAGAATTTTTCTGAGTACCAAGAAATAGCAGTACAAAACCATTCTTCTGAAACA
  TGCCAACATGTGTCTGAACCTGAAGACCTCTCTCCTAAAATGTACCAAGAAATATCTGTACTTCAAGAC
  AATTCTTCCAAAATATGCCAAGACATGAAGGAACCTGAAGACAACTCTCCTAACACATGCCAAGTAATA
  TCTGTAATTCAAGACCATCCTTTCAAAATGTACCAAGATATGGCTAAACGAGAAGATCTGGCTCCTAAA
  ATGTGCCAAGAAGCTGCTGTACCCAAAATCCTTCCTTGTCCAACATCTGAAGACACAGCTGATCTGGCA
  GGATGCTCTCTTCAAGCATATCCAAAACCAGATGTGCCTAAAGGCTATATTCTTGACACAGACCAAAAT
  CCAGCAGAACCAGAGGAATACAATGAAACAGATCAAGGAATAGCTGAGACAGAAGGCCTTTTTCCTAAA
  ATACAAGAAATAGCTGAGCCTAAAGACCTTTCTACAAAAACACACCAAGAATCAGCTGAACCTAAATAC
  CTTCCTCATAAAACATGTAACGAAATTATTGTGCCTAAAGCCCCCTCTCATAAAACAATCCAAGAAACA
  CCTCATTCTGAAGACTATTCAATTGAAATAAACCAAGAAACTCCTGGGTCTGAAAAATATTCACCTGAA
  ACGTATCAAGAAATACCTGGGCTTGAAGAATATTCACCTGAAATATACCAAGAAACATCCCAGCTTGAA
  GAATATTCACCTGAAATATACCAAGAAACACCGGGGCCTGAAGACCTCTCTACTGAGACATATAAAAAT
  AAGGATGTGCCTAAAGAATGCTTTCCAGAACCACACCAAGAAACAGGTGGGCCCCAAGGCCAGGATCCT
  AAAGCACACCAGGAAGATGCTAAAGATGCTTATACTTTTCCTCAAGAAATGAAAGAAAAACCCAAAGAA
  GAGCCAGGAATACCAGCAATTCTGAATGAGAGTCATCCAGAAAATGATGTCTATAGTTATGTTTTGTTT
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >Peptide sequence encoded by RC214122
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MDLGKDQSHLKHHQTPDPHQEENHSPEVIGTWSLRNRELLRKRKAEVHEKETSQWLFGEQKKRKQQRTG
  KGNRRGRKRQQNTELKVEPQPQIEKEMEKALAPIEKKTEPPGSITKVFPSVASPQKVVPEEHFSEICQE
  SNIYQENFSEYQEIAVQNHSSETCQHVSEPEDLSPKMYQEISVLQDNSSKICQDMKEPEDNSPNTCQVI
  SVIQDHPFKMYQDMAKREDLAPKMCQEAAVPKILPCPTSEDTADLAGCSLQAYPKPDVPKGYILDTDQN
  PAEPEEYNETDQGIAETEGLFPKIQEIAEPKDLSTKTHQESAEPKYLPHKTCNEIIVPKAPSHKTIQET
  PHSEDYSIEINQETPGSEKYSPETYQEIPGLEEYSPEIYQETSQLEEYSPEIYQETPGPEDLSTETYKN
  KDVPKECFPEPHQETGGPQGQDPKAHQEDAKDAYTFPQEMKEKPKEEPGIPAILNESHPENDVYSYVLF
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Recombinant protein using RC214122 also available, TP314122
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_197978
• ORF Size: 1449 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 2306 bp
• RefSeq ORF: 1455 bp
• Locus ID: 55363
• UniProt ID: Q9BXL5
• Cytogenetics: 9q22.33
• MW: 55.3 kDa
• Gene Summary: Regulates the proliferation and differentiation of hematopoietic cells. Overexpression block the TPA-induced megakaryocytic differentiation in the K562 cell model. May also prevent cell apoptosis through the activation of the nuclear factor-kappa B (NF-kB).[UniProtKB/Swiss-Prot Function]